• Plant Resources
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• 제6권2호
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• pp.89-93
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• 2003

This study was conducted to develop the clonal propagation technique through in vitro culture using basal stem explants in Phalaenopsis hybrid grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when basal stem explants were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 ml/L coconut water, 1 mg/L NAA, 5 mg/L 2iP and 2.5g/L gel rite. PLBs transferred to Hyponex medium were regenerated to plantlets. Plantlets transferred to plastic pots containing spagnum moss were developed and successfully acclimatized under greenhouse. The flower was bloomingly opened in plants regenerated from basal stem explants. The flower was not different from both mother plant and plant induced through clonal propagation of Phalaenopsis hybrid.

• 식물조직배양학회지
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• 제22권2호
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• pp.105-110
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• 1995

Petunia hybrida와 Nicotiana sanderae의 원형질체를 융합하여 체세포잡종식물을 얻고자 융합산물의 배양에 의해 얻은 개체들에 대해 유전분석을 하였다. 재분화 개체중 융합된 것으로 인정되는 개체의 peroxidase pattern은 두 모본의 band를 모두 가진 개체(A-D patterns)에서 나타난 band중 p. hybrida에서 나타난 band 이외에 최상부에 1개의 band가 추가되었는데 이는 융합을 판별할 수 이는 중요한 marker 로서 가능성이 있으며, aspartate aminotransferase isoenzyme pattern은 두 모본의 양상이 모두 나타나거나 N. sanderae의 상부 band가 소실된 개체도 있었다. 염색체 검경에서 P.hybrida는 2n=14, M sanderae는 2n=18개로 나타났으며, 동위효소검정에서 체세포 잡종일 것으로 추정되는 개체들은 2n=32-36으로 나타났다. 표현형 관찰에서 체세포잡종으로 인정되는 개체의 형태적 특징은 두 모본의 중간형태를 나타내었다.

• Journal of Plant Biology
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• 제36권1호
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• pp.35-42
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• 1993

This study deals with the effect of dwarf growth for the wood and the structural changes of cambium in Pinus koraiensis subjected to water stress. The length and wall thickness of tracheary elements in dwarf trees was shorter and thicker than that in normal trees. The redial width and cell number of the annual rings are narrower and smaller in dwarf trees than those in normal trees. In serial tangential sections, the frequency of anticlinal division is low and loss of cambial initials is small in dwarf trees. The length of cambial initials in water deficit, trees are slightly shorter than that in normal trees, and the instructive growth of cambial initials during differentiation of xylem mother cells is inactive in dwarf trees. Thus, it is interpreted that the shortening of tracheary elements in dwarf trees is due to the fact that the length of cambial initials are shortened and the intrusive growth of those during differentiation of xylem mother cells is inactive. The structural variations in the wood of naturally occurring dwarf trees are similar to those of trees subjected to artificial water stress. Therefore, it is suggested that the variation of xylem element in dwarf trees are related to water conditions.

• Plant Biotechnology Reports
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• 제2권4호
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• pp.245-252
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• 2008

The genetic status of somatic embryo-derived plantlets of Cymbopogon flexuosus was examined by randomly amplified polymorphic DNA (RAPD) analysis. Auxins such as 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1-4 mg/l) were used in Murashige and Skoog (MS) medium for induction of calli from rhizomatous explants of Cymbopogon flexuosus. Optimum calli were induced on MS medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) (3.5 mg/l) alone or in combination with $N^6-benzyladenine$ (2 mg/l). Somatic embryogenesis was achieved from long term calli when cultured on MS medium containing 2, 4-dichlorophenoxyacetic acid (2, 4-D) (2 mg/l) along with $N^6-benzyladenine$ (BA) (1-2 mg/l). Regeneration was achieved when freshly induced embryogenic calli were sub-cultured on MS medium supplemented with $N^6-benzyladenine$ (3 mg/l) alone. Long-term cultured embryos showed profuse minute rooting on regeneration medium supplemented with N6 -benzyladenine (3 mg/l). Microshoots were rooted in the presence of indole-butyric acid (IBA) (2 mg/l). DNA samples from the mother plant and 18 randomly selected regenerated plants from a single callus were subjected to RAPD analysis with 6 arbitrary decamer primers for the selection of putative somaclones. A total of 64 band positions were scored, out of which 19 RAPD bands were polymorphic. From genetic similarity coefficient based on RAPD band data sharing, it was found that the majority of the clones were almost identical or more than 92% similar to the mother plant, except CL2 and CL9 (66%) which showed highest degree of genetic change with CL2 and CL9 showing presence of two non-parental bands each.

• 한국약용작물학회지
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• 제26권5호
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• pp.354-361
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• 2018

Background: This study was conducted to investigate the growth characteristics, root yield and curcuminoid content of turmeric (Curcuma longa L.), cultivated using mother seed rhizomes (MR) and finger seed rhizome (FR) of different sizes. MR are attached to the stem, and FR are connected to the MR, and are used as a general seed rhizome. Methods and Results: Seed rhizomes of different types and sizes were used: large, medium and small for FR, and large, half-sized, and small for MR. These were assigned to the experimental groups and cultivated under greenhouse conditions. The growth characteristics, root yield, and curcuminoid content did not show clear difference between MR and FR, but suggest that the larger seed rhizomes (above 30 g) could have higher root yields. On average, harvested mother rhizomes (HMR) contained more curcuminoid than harvested finger rhizomes (HFR), while the yield of HFR was higher than that of HMR. The higher weight of harvested roots correlated significantly with elevated curcuminoid content. Conclusions: The two seed rhizomes, MR and FR, did not differ in root yield and curcuminoid contents, but larger seed rhizomes may produce better root yields. This suggest that the optimum seed rhizome is larger FR, to produce higher yields and quality in turmeric root production.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.201-207
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• 2003

Tissue culture techniques arguably are an important approach for ex situ conservation of rare and endangered plant species. However, there is utmost importance on maintaining the genetic integrity of the introduced plants especially in tree species. To examine the genetic integrity of the micropropagated plants, we randomly screened few hardened plants of Syzygium travancoricum, a critically endangered tree taxon, using Randomly Amplified Polymorphic DNA (RAPD) markers. Twenty-three random. primers were tried and twenty-five polymorphic loci were identified. The dendrogram based on the Unweighted Pair-Group Method Arithmetic Average and Nei's similarity index depicted about 97% homology between the mother plants and micropropagated plants. Further, an attempt was made to reintroduce the micropropagated plants in the wild. Over three hundred small trees could be successfully established.

• 한국자원식물학회지
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• 제32권1호
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• pp.19-28
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• 2019

An efficient shoot regeneration condition for pea cv. 'Sparkle' was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

• 생물환경조절학회지
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• 제29권4호
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• pp.337-343
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• 2020

9월 15일경 본포에 정식하는 딸기 촉성재배에 필요한 묘령 75일 이상의 정식묘를 삽목을 이용해 육묘하기 위해, 어미묘의 정식 시기와 런너의 방임 시기에 따른 삽수의 생산성을 검토하였다. 시험 품종은 국내에서 육성된 '매향(Maehyang)', '죽향(Jukhyang)' 및 '금실(Kuemsil)' 등 3품종을 사용하였다. 시험 1은 어미묘 정식 시기에 따른 삽수 생산량을 조사하기 위해 20일 간격으로 2월 28일, 3월 20일 및 4월 9일에 어미묘를 정식하였으며, 6월 4일부터 7월 1일까지 2~3회에 걸쳐 삽수를 채취하였다. 시험 2는 어미묘의 런너 방임시기에 따른 삽수 생산량을 조사하기 위해 3월 5일에 어미묘를 정식하였으며, 정식 후 20일, 40일 및 60일 간격으로 런너를 방임하였다. 그리고, 5월 29일부터 6월 26일까지 1~3회에 걸쳐 삽수를 채취하였다. 어미묘 정식 시기별 자묘 발생량을 비교한 결과, 2월 28일 정식 구가 3월 20일 및 4월 9일 정식 구 대비 품종 별로 각각 29~45%, 114~165% 더 많았다(시험 1). 어미묘의 런너 방임시기별 자묘 발생량은 정식 20일 후부터 방임한 것이 40일 및 60일 후 방임한 처리구 대비 품종 별로 각 60~77%, 104~176% 증수된 것으로 나타났다(시험 2). 결과적으로, '매향', '죽향', 및 '금실' 3품종 모두 9월 15일경 본포 정식에 필요한 묘를 삽목으로 번식할 경우, 어미묘를 2월 하순경 일찍 심는 것이 삽수 발생량이 가장 많았으며, 어미묘에서 발생되는 런너는 정식 후 20일까지 제거한 후 방임하는 것이 삽수 생산에 유리한 것으로 조사되었다. 이 연구 결과는 국내 육성 신품종의 생력 육묘 및 품질 향상을 위한 효율적인 육묘시스템의 개선을 위해 활용하고자 한다.

• The Plant Pathology Journal
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• 제33권6호
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• pp.561-571
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• 2017

Prospecting of local grapevine (Vitis vinifera L.) germplasm revealed that Tunisia possesses a rich patrimony which presents diversified organoleptic characteristics. However, viral diseases seriously affect all local grapevine cultivars which risk a complete extinction. Sanitation programs need to be established to preserve and exploit, as a gene pool, the Tunisian vineyards areas. The presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA), were confirmed in a Tunisian grapevine cultivar using serological and molecular analyses. The association between GRSPaV and GVA viruses induces more rugose wood symptoms and damages. For this reason the cleansing of the infected cultivar is highly advisable. Direct and recurrent somatic embryos of cv. 'Hencha' were successfully induced from filament, when cultured on $Ch{\acute{e}}e$and Pool (1987). based-medium, enriched with $2mg1^{-1}$ of 2,4-dichlorophenoxyacetic acid and $2.5mg1^{-1}$ of Thidiazuron, after 36 weeks of culture. After six months of acclimatization, RT-PCR carried on 50 somaplants confirmed the absence of GVA, GRSPaV as well as GLRaV-3 viruses in all somaplants. Ampelographic analysis, based on eight OIV descriptors, was carried out on two years acclimated somaplants, compared to the mother plant. Results demonstrated that the shape and contours of 46 somaclones leaves are identical to mother plant leaves and four phenotypically off-type plants were observed. The healthy state of 100% 'Hencha' somaclones and the high percentage of phenotypically true-to-type plants demonstrate that somatic embryogenesis is a promising technique to adopt for grapevine viruses elimination.

• 한국식생활문화학회지
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• 제3권3호
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• pp.245-258
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• 1988

A food life style itself is substrative relations with culture, and is concerned with our daily life. Especially a food taboo comes from the intelligence which resulted from an ancestor's life experience. Accordingly, we can say a food taboo began with human appearance, settled in convention of folklore society, and forms it's own boundary todays. Since a practice of a food taboo is combinded firmly with various sociological factors such as religion, custom, and so on, it is very hard to change, even though the belife of a food taboo is not true. According to the result, first, significant factors effecting on a practice of a food taboo was a level of age, a level of education, religion, family pattern, behavior of subject and her mother for food life management, education of subject and her mother, and religion of subject and her mother. Second, in analysis of factors according to classification of food taboo a practice of a food taboo which classified to food of animal, food of plant and the other food showed significant differences, comparing religion of subject with her mother. Third, a result examined practice frequency and review of science, non science indicated that foods such as Egg of Globefish, Lettuce, Coffee, Persimmon, dried Persimmon, Soybean and Sugar are tabooed on the basis of science, and foods such as Vinegary food, Thieved food, Soup of Tangle and Dogmeat tabooed on the basis of non science. But in the case of Puja, it's basis of science is not identified. These food taboos are tabooed by more then 50% of subjects including who answerd 'there is some case to practice it' Therefore, we should continue analysis of science for the reason of a food taboo, at the same time, provide the origin and try to have a rational food life.