• 한국식품과학회지
• /
• 제47권1호
• /
• pp.87-94
• /
• 2015

비가열 살균 기술 중 본격적인 상업적 실용화를 눈앞에 두고 있는 고전압 펄스 전기장에 의한 미생물의 사멸 기작에 대해 살펴보았다. 세포 현탁액을 고전압 펄스 전기장 처리하였을 경우 처리 시간이나 전기장의 세기가 증가할수록 세포 외액으로 세포내 물질의 유출이 증가하였으며, 세포막 투과성의 변화로 인하여 $K^+$, $Na^+$등이 이온 성분의 유출도 나타났다. 염색시약에 의한 세포의 염색에서 처리시간이 증가함에 따라 염색되는 세포의 수가 증가하였으며, 전자현미경에 의한 세포의 관찰 결과 고전압 펄스 전기장 처리를 받은 세포의 경우 처리 받지 않은 것에 비해 표면이 거칠고 굴곡이 있었으며, 세포막이 터져 세포내 물질이 외부로 유출되고 형태가 일그러진 것이 관찰되었다. 항생물질 첨가에 따른 회복 실험에서 고전압 펄스 전기장 처리에 의해 세포의 단백질 합성 체계에 손상을 입었으며, chromosomal DNA의 분리를 통한 DNA의 손상여부 관찰 결과 약 27.3%의 DNA의 손상이 발생했음을 알 수 있었다. 따라서 고전압 펄스 전기장 처리가 세포벽이나 세포막의 손상뿐만 아니라 대사 체계와 DNA에도 손상을 주는 것을 확인할 수 있었다.

• 전자공학회논문지
• /
• 제54권1호
• /
• pp.70-79
• /
• 2017

본 논문에서는 도로안내표지판 내의 거리정보를 빠르고 정확하게 획득하는 방법을 제안한다. 제안된 방법은 표지판의 인식, 거리를 획득하기 용이한 전 처리 과정, 거리정보를 습득하는 것으로 구성된다. 표지판의 인식은 여러 가지 잡음을 해결하기 위해 감마 보정을 포함한 색상검출을 사용하였으며, 거리정보를 용이하게 획득하기 위해서 직선 인자를 이용한 기울기 보정과 고속 푸리에변환을 이용한 해상도 보정을 적용하였다. 거리정보를 습득하는 과정은 모폴로지 연산을 통해 영역을 부각하고 레이블링, 템플릿 매칭을 사용하였다. 이러한 과정을 통해 도로안내표지판의 거리정보를 습득하여 분기점까지 남은 거리를 출력하는 시스템을 제안하였다. 결과적으로 연산속도 개선에 중점을 두어 실시간으로 처리할 수 있는 시스템에 사용 가능하며, 그 결과 프레임 당 평균 0.46초의 속도를 가지며, 정확도에서도 유사도 0.65의 수치를 갖는다.

• 한국산업정보학회논문지
• /
• 제12권2호
• /
• pp.49-59
• /
• 2007

본 논문은 시맨틱 웹의 실현을 위해서는 필수적인 작업인 웹문서의 의미를 자동으로 태깅할 수 있는 시스템에 관한 것이다. 웹상의 방대한 자원을 일일이 사람이 수작업으로 의미를 태깅한다는 것은 사실상 불가능하기 때문에 한국어 웹문서를 대상으로 대량의 학습 데이터를 수집하고 자연어처리 기법과 시소러스를 이용하여 특징을 추출한 후 SVM 기계학습을 통하여 개념분류기를 구축하였다. 한국어의 특징을 파악하여 의미 태깅에 필요한 특징 정보를 추출하기 위해서 형태소 분석과 구문 분석을 하였다. 추출된 특징정보는 가도카와 시소러스의 의미코드를 이용하여 학습벡터로 구성되는데, 이는 유사한 단어나 구를 하나의 개념코드로 매핑하여 시스템의 재현율을 높이는 역할을 하게 된다. 실험결과 자동 의미 태깅 분야에서 본 접근방법의 가능성을 확인할 수 있었다.

• 천문학회지
• /
• 제46권5호
• /
• pp.191-200
• /
• 2013

Full-disk solar images are provided by many solar telescopes around the world. However, the observed images show Non-Radial Variation (NRV) over the disk. In this paper, we propose algorithms for detecting distortions and restoring these images. For detecting NRV, the cross-correlation coefficients matrix of radial profiles is calculated and the minimum value in the matrix is defined as the Index of Non-radial Variation (INV). This index has been utilized to evaluate the H images of GONG, and systemic variations of different instruments are obtained. For obtaining the NRV's image, a Multi-level Morphological Filter (MMF) is designed to eliminate structures produced by solar activities over the solar surface. Comparing with the median filter, the proposed filter is a better choice. The experimental results show that the effect of our automatic detection and restoration methods is significant for getting a flat and high contrast full-disk image. For investigating the effect of our method on solar features, structural similarity (SSIM) index is utilized. The high SSIM indices (close to 1) of solar features show that the details of the structures remain after NRV restoring.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권1호
• /
• pp.17-22
• /
• 2004

A bacterial strain WJ-98 found to produce active extracellular keratinase was isolated from the soil of a poultry factory. It was identified as Paracoccus sp. based on its 16S rRNA sequence analysis, morphological and physiological characteristics. The optimal culture conditions for the production of keratinase by Paracoccus sp. WJ-98 were investigated. The optimal medium composition for keratinase production was determined to be 1.0% keratin, 0.05% urea and NaCl, 0.03% K$_2$HPO$_4$, 0.04% KH$_2$PO$_4$, and 0.01% MgCl$_2$$.$6H$_2$O. Optimal initial pH and temperature for the production of keratinase were 7.5 and 37$^{\circ}C$, respectively. The maximum keratinase production of 90 U/mL was reached after 84 h of cultivation under the optimal culturing conditions. The keratinase from Paracoccus sp. WJ-98 was partially purified from a culture broth by using ammonium sulfate precipitation, ion-exchange chromatography on DEAE-cellulose, followed by gel filtration chromatography on Sephadex G-75. Optimum pH and temperature for the enzyme reaction were pH 6.8 and 50$^{\circ}C$, respectively and the enzymes were stable in the pH range from 6.0 to 8.0 and below 50$^{\circ}C$. The enzyme activity was significantly inhibited by EDTA, Zn$\^$2+/ and Hg$\^$2+/. Inquiry into the characteristics of keratinase production from these bacteria may yield useful agricultural feed processing applications.

• Applied Microscopy
• /
• 제21권2호
• /
• pp.96-116
• /
• 1991

The authors studied the morphological distinctions of each of the epidermal layers and the time of appearance of the keratohyalin granules and tonofilaments by the processing of development. The skins were obtained from fetal rats at the age of 14th, 16th, 17th, 18th, 19th and 20th day of gestation, of 1st and 3rd day of neonatal life and of 4th week after birth. The specimens were staind with uranyl acetate and lead citrate. The results obtained were as follows. 1. On the 16th-gestation day, the intermediate layer which contained numerous ${\alpha}-and\;{\beta}$-glycogen particles was appeared, and hemidesmosomes and desmosome were observed as well. 2. Tonofilaments were first observed on the 17th gestation day. 3. Above-mentioned intermediate layer was differentiated into the granular layer and the spinous layer on the 18th-gestation day. Keratohyaline granules were appeared in association with the ribosomes and the tonofilaments and the compound granules were lipoid granules which were surrounded by ribosomes at the periphery. 4. Ultimately, keratinization began to take place from the 20th-gestation day. At the age of 4th week, the thickeness of epidermis and the amount of keratohyaline granule and tonofibrils were decreased. It is consequently suggested that in the differentiation process of the rat epidermis, keratinization begins after formation of hemidesmosomes and desmosomes, from which the tonofilaments are formed and after keratohyaline granules are formed. Therefore appearance of the keratohyaline granules and formation of the tonofilament appears to have a close relations with the keratinization process of the rat epidermis.

• 열처리공학회지
• /
• 제7권4호
• /
• pp.244-252
• /
• 1994

The morphological changes of primary solid particles as a fuction of process time on Al-Si alloys during semi-solid state processing with a shear rate of 200s were studied. In hypereutectic Al-15.5wt%Si alloy, it was observed that primary Si crystals are fragmented in the early stage of stirring and morphologies of primary Si crystals change from faceted to spherical during isothermal shearing for 60 minutes. In quaternary Al-12.5wt%Si-2.9wt%Cu-0.7wt%Mg alloy system, it was observed both primary silicon and ${\alpha}$-alumunum particles. Microstructural evolution of primary Si crystals was similar to that of the hypereutectic Al-Si alloy but equiaxed ${\alpha}$-Al dendrites are broken into nearly spherical at the early stage of shearing and later stage of the isothermal shearing ${\alpha}$- Al particles are slightly coarsoned by Ostwald ripening. Mechanical properties of Al-Si-Cu-Mg alloy were compared to those from other processes (squeeze casting and gravity casting). After T6 heat treatment, comparable values of hardness were obtained while slightly lower compressive strength values were observed in rheocast alloy. The elongation, on the other hand, exhibited significant increasement of 15% over gravity cast alloy.

• 한국식품과학회지
• /
• 제29권1호
• /
• pp.51-56
• /
• 1997

겨자의 항균성 및 식품보존제로의 이용가능성을 조사하기 위하여 겨자의 첨가에 따른 냉면육수의 보존 중 그 효과를 조사하고, 대장균의 형태변화를 현미경으로 관찰한 결과는 다음과 같다. 겨자를 첨가시킨 냉면육수의 적정산도는 처음에 겨자 무첨가 육수에 비하여 높았으나 24시간 보존 후에는 겨자 무첨가 육수보다 낮게 나타났으며, 겨자 첨가 냉면육수의 생균수 및 대장균수는 처음에 겨자 무첨가시보다 감소하였으며, 겨자 첨가농도가 높을수록 감소하는 폭이 크게 나타났고, VBN 함량은 보존기간이 지남에 따라 증가하였고, 겨자가 첨가된 냉면육수가 겨자 무첨가보다 낮게 나타났으며, 겨자첨가 농도가 높을수록 더욱 낮게 나타났다. 겨자 물추출물로 처리한 대장균을 전자현미경으로 관찰한 결과 균체표면이 수축되고 표층구조가 허물어져 심한 형태학적 변화를 나타내었다.

• The Plant Pathology Journal
• /
• 제26권2호
• /
• pp.121-129
• /
• 2010

We evaluated microbial populations and aflatoxin production in unhulled and white rice from rice processing complexes of the National Agricultural Cooperative Federation in five regions in Korea and identified three predominant Aspergillus species. Fungal and bacterial populations in rice samples were significantly different between regions in 2007. Aflatoxins were also detected and varied at the levels of 2.45 - 3.43 ng per g unhulled rice grain and 1.29 - 2.09 ng per g white rice grain. Unhulled rice generally detected higher level of aflatoxins than white rice regardless of sampling regions; however, no significant differences were found in Anseong and Cheonan in 2005 and Cheonan and Gimpo in 2007. Aflatoxin production between sampling regions was not different regardless of rice type and sampling year. Although the fungal diversity was highly distinct from region to region, three Aspergillus isolates were predominant in the rice samples; thus, representative isolates AC317, AF57, and AF8 were selected and identified based on their morphological and molecular characteristics. Consequently, isolates AC317, AF57, and AF8 were identified as A. candidus, A. flavus, and A. fumigatus, respectively. These fungi can produce mycotoxins that are harmful for consumers and thus it is important to detect and reduce the population of storage fungi in rice.

• 한국안전학회지
• /
• 제32권6호
• /
• pp.61-67
• /
• 2017

Suspended sediment transport plays principal roles in morphological process of natural coastals. It is needed to understand the reason why interaction characteristics of solitary wave and suspended sediment. The present study shows that suspended sediment pickup derived on solitary wave celerity. The 2D prismatic open channel length is 12 m, width is 0.8 m, height is 0.75 m and slope is 1/6. Generation of solitary wave is used by rapidly opening the sluice gate. Bottom surface sediments are laid movable slope section by 0.03 m thickness and experimental sediments are used anathracite and jumoonjin sand. Techniques of suspended sediment pickup rate are designed equipment ASC(Absorptive Suspended sediment Collector). It could directly absorb 5 points suspended sediment by channel water depth. Solitary wave celerity is measued by ADV(Acoustic Doppler Velocimeter). Mounted two video cameras(Model No. : Sony, HDR-XR550) are used to image processing of suspended sediment concentration and turbidity. Suspended sediment pikcup rate(Einstein, 1950) is analyzed to nondimensionalization based on solitary wave celerity. The suspended sediment pickup rate is suggested that more effective plunging breaking type than spilling. The results indicates fundamental suspended sediment transport mechanism between solitary wave celerity and suspended sediment pickup based on laboratory experiments. Finally, the present study suggests that suspended sediment pickup rate by solitary wave is used only characteristics of sediment and solitary wave celerity.