• Journal of Embryo Transfer
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• v.30 no.3
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• pp.213-218
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• 2015

In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming at experimental applications. For developing the efficient method to identify the stage of sperm cells, the morphological characteristics of sperm cells were investigated by staining the cells with blue fluorescent dye Hoechst 33258, and a criterion for male germ cell classification was elicited from results of the previous investigation, then the efficiency of the criterion was verified by applying it to assort the germ cells recovered from male mice in age from 6 to 35 days. As morphological characteristics, spermatogonia significantly differed from spermatocytes in size, appearance and fluorescent patches of nucleus, and spermatids could also be distinguished from spermatozoa by making a difference in the volume and shape of nucleus and the shape and fluorescence of tail. Aforesaid criterion was applicable for classifying in vitro cultured sperm cells by verifying its efficiency and propriety for assorting the stages of testicular germ cells. However, the fluorescent staining showed that germ cells in mouse testis should be dramatically differentiated and developed at 21 days and 35 days of age, which were known as times of sexual puberty and maturity in male mice, respectively. In conclusion, the results indicated that this simple criterion for sperm cell classification using fluorescence staining with Hoechst 33258 may be highly efficient and reasonable for spermatogenesis study.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• Journal of radiological science and technology
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• v.21 no.1
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• pp.69-78
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• 1998

In this study, I examined the morphological influence of radiation on rats stomach tissue, the difference between NPO and CON(contrast) in the rats stomach was measured after a single irradiation to 15 Gy dose, the results follow; In histological observation; focal congestion and epithelial denudation were observed from NPO rats of a day after irradiation. Fibrosis was showed from both of NPO and CON rats of 28day after irradiation, but in it's area, NPO rats have been more wider than CON rats. In PAS reaction; secreted mucin was reacted very weakly from both of NPO and CON rats of 7 days and 14 days after irradiation, whereas CON rats of 28 days after irradiation were reacted like to control rats. On the basis of this results, we know obviously that NPO will be a factor of stomach abnomalities while hospital patients are treated with high-energy irradiation.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.44 no.4
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• pp.69-76
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• 2012

The bamboo fibers from three Korean bamboo species, Moso, Henon and Timber bamboo, were prepared by disintegration after thermal treatment. The samples were prepared according to the age of growth; bamboo shoots, 20 days and 50 days of growth. The fiber quality was also investigated by morphological and chemical analyses. There was no big difference in the dry mass of fibers among the three bamboo species. However, the dry mass of fibers from 50 days of growth was increased by 34%, whereas it was ranged in 9-20% in the case of fibers from bamboo shoots and 20 days of growth. In the results of morphological analysis, the fibers could be classified as the fibers from metaxylem, the fibers from parenchyma and the fines produced during disintegration. The fibers from 50 days of growth were separated from metaxylem, whereas the fibers from bamboo shoots and 20 days of growth were mainly consisted of fibers from metaxylem and parenchyma. The chemical analysis of fibers showed that the contents of carbohydrates, lignin and extractives were not much affected by thermal treatment.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.889-894
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• 2005

This experiment was carried out to investigate the effects of simulated acid rain (SAR) in the shoot growth and internal structure of Arabidopsis thaliana. In the shoot growth, the plants treated with SAR (pH 3.0) for 15 days showed no morphological change compared to the control plants. Some change was observed in the internal structure of the stems: the epidermis and cortex tissues of the stems treated with SAR were partly damaged. The plants treated with SAR showed no noticeable difference compared to the control plants, but morphological changes were observed in the leaf. The leaves of the plants treated with SAR showed many white necrotic spots on the part of upper epidermis. A light microscopic examination of the leaves with necrotic spots showed that the upper epidermis was severely compressed with the damaged cuticle layer and the mesophyll cells were also damaged and compressed. However, noticeable structural change of vascular bundle cells was not observed.

• Journal of Korean Society of Forest Science
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• v.47 no.1
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• pp.27-36
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• 1980

To establish an easy classification method of bamboos observation of the shapes of vascular bundle sheath of Sasa, one of the important natural resources available in Korea, was made on the basis of vascular bundle sheath of bamboo culms because Sasa has a unique vascular bundle sheath as discussed by Grosser and Liese et al. Morphological characteristics of Saw observed are summarized as follows: 1. The thickness of culm wall showed no distinctive difference between upper and low parts of culms of Sasa grown at the ground level. 2. In relation to the taxonomical classification of bamboo, Sasa showed a and a' type but did not e.f.h. and e' type was not identified. 3. Two typical types of bamboo vascular bundle sheath, i.e. b.c.d.e. and g type and d and e type, were found in Sasa but sometimes b.c.g. type was not observed. 4. The results mentioned above seem to be an important key for classification of Sasa.

• Journal of Korean Society of Forest Science
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• v.34 no.1
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• pp.47-56
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• 1977

I have investigated and compared the morphology of vascular bundle shown in the section of culm wall of bamboo trees growing on Mt. Jiri which were classified by Grosser and Liese with their methods of morphological classification. The results obtained were as follows: 1. It was shown that there are no b.g.i. types of bamboo classified by Grosser and Liese among the bamboo trees on Mt. Jiri (Phyllostachys and Sasa). 2. As for the thickness of the culm wall in the culm, it was shown that the culm wall of the Phyllostachys becomes thinner in proportion to its nearness to the upper part of the tree, but no distinctive difference appeared in the Sasa. 3. The c, d, and e types of Sasa were the same as those of the Phyllostachys, but there was a vascular bundle type of the a' type, which was quite different from that of the Phyllostachys. 4. It was shown that the a', d, and e types of Sasa were distributed in a zone less than 500m above sea level, but no a' type was distributed in the high mountain area except for the c, d and e types which ranged from 600m to 1000m above sea level. Such facts mean that the vascular bundle sheath has changed in quantity because of the height of mountain. 5. In general, as compared with the Phyllostachys, the Sasa (types a, c, d and e which included a new type a) have fewer vascular bundles. 6. Considering the above results, it is thought that not by the current Sasa classification method based on observation of the the study of Sasa form the outside, but by a new method of classification based on the aspect of the physiological construction as seen from the inside wall is advanced. I believe this new method of classification to be a first step towards an epoch-making methodological advance and encourage the further study of it.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.1
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• pp.8-19
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• 2023

Adzuki beans have gained popularity in recent years due to their health benefits. Breeding of Adzuki beans is less favorable than with other legumes due to low genetic diversity. This study aimed to evaluate the genetic diversity of 252 adzuki bean germplasms from China, Japan, and Korea using 18 agro-morphological parameters and comparing their performance to three prominent Korean cultivars: Geomguseul, Arari, and Chungjupat. Leaf shape, pod color, and seed coat color were among the qualitative traits that showed wide variations. The quantitative variables also showed wide variations among adzuki bean germplasms. Although there was no significant difference (p < 0.05), the average rate of germination declined in the order of Korean (91.44%) > Chinese (91.31%) > Japanese (87.47%) adzuki beans. Chinese adzuki beans needed fewer days to flower (DF, 58.22 days) and days to mature (DM, 107.13 days), which varied significantly compared to the Korean and Japanese adzuki beans (p < 0.05). The average number of pods per plant (PPP) and one-hundred seeds weight (HSW) were higher in Japanese adzuki beans compared to the Korean and Chinese adzuki beans although the variation of each was not significant. Almost 29.76% of the accessions had early-blooming flowers, 3.97% were premature, 21.43% produced more PPP, and 3.97% yielded more SPP compared to control cultivars. Results of hierarchical cluster and principal component analyses revealed three clusters with significant variation in all quantitative variables except for RG (p < 0.05). The key factors in multivariate analyses were DF, DM, and HSW. Our study investigated the genetic diversity of adzuki bean accessions and identified ten early maturing and ten high PPP-yielding accessions. Our findings would help farmers and breeders to select the top-performing accessions that can provide them with various options.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.378-383
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• 2023

A re-examination of Luciogobius guttatus was conducted using 84 samples, which lives on all coastal waters of Korea. As a result, 41 specimens (31.3~54.4 mm SL) collected from the west and south coasts were classified as L. martellii. The morphological features of L. martellii as follows: number of abdominal vertebrae 16; total number of vertebrae 35~37; dorsal fin base length longer than upper caudal peduncle length or equal; one free ray on its upper pectoral fin; and edge of tail fin white. Among these, the most prominent morphological difference in L. guttatus is comparing the dorsal fin base length and upper caudal peduncle length, which is also well observed in syntypes of L. martellii. We proposed a new Korean name, "Geom-jeong-mi-kken-mang-duk" referring to their color.

• The Journal of Advanced Prosthodontics
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• v.16 no.3
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• pp.189-199
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• 2024

PURPOSE. This study was conducted to evaluate the effects of plasma treatment of sandblasted and acid-etched (SLA) titanium implants on surface cleansing and osseointegration in a beagle model. MATERIALS AND METHODS. For morphological analysis and XPS analysis, scanning electron microscope and x-ray photoelectron spectroscopy were used to analyze the surface topography and chemical compositions of implant before and after plasma treatment. For this animal experiment, twelve SLA titanium implants were divided into two groups: a control group (untreated implants) and a plasma group (implants treated with plasma). Each group was randomly located in the mandibular bone of the beagle dog (n = 6). After 8 weeks, the beagle dogs were sacrificed, and volumetric analysis and histometric analysis were performed within the region of interest. RESULTS. In morphological analysis, plasma treatment did not alter the implant surface topography or cause any physical damage. In XPS analysis, the atomic percentage of carbon at the inspection point before the plasma treatment was 34.09%. After the plasma treatment, it was reduced to 18.74%, indicating a 45% reduction in carbon. In volumetric analysis and histometric analysis, the plasma group exhibited relatively higher mean values for new bone volume (NBV), bone to implant contact (BIC), and inter-thread bone density (ITBD) compared to the control group. However, there was no significant difference between the two groups (P > .05). CONCLUSION. Within the limits of this study, plasma treatment effectively eliminated hydrocarbons without changing the implant surface.