• Korean Journal of Ichthyology
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• v.18 no.4
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• pp.339-346
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• 2006

In this study we conducted artificial hybridization between two sibling species, Rhodeus uyekii and R. notatus, and observed the morphological characteristics in early developmental stage. The two species showed difference in egg shape having rhombus and club respectively. The morphology of yolk of larva just hatched also showed well specific characteristics, that is the bean chaff type for R. uyekii (U type) and anchor type for R. notatus (N type). The rate of fertilization between female R. uyekii and male R. notatus (UN type) and between female R. notatus and male R. uyekii (NU type) were complete and its hatching rate were very high, 71.6% for UN type and 97.5% for NU type. The differences occurred in the yolk shape of hatched larvae for each combination of hybrids. In the group of UN, U type of larvae were found very rarely, but almost all the larvae showed intermediate shape polarized to NN type. Similar phenomenon was observed in the NU having intermediate polarized to UU, but without any NN type. These hybrids will be analyzed for their external morphology, sex ratio, the function of sexual organ and karyology after they grown up to adult.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.106-111
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• 2005

For breeding of a new rootstock for eggplant production, somatic hybrids between two species, Solanum integrifolium and S. sanitwongsei were obtained through protoplast fusion. The former species has been commonly used for rootstock for eggplant production in Japan. Eggplants on these rootstocks are more productive than ungrafted plants, but are susceptible to bacterial wilt caused Ralstonia solanacearum. While the latter species is resistant, the growth of eggplants on this rootstock is rather slow and low yield. Protoplast of both species were isolated from cotyledons, and inactivated with iodoacetamide or UV-irradiation, then fused electrically. The fused products were then cultured. Regenerated plantlets were then transplanted on soil then maintained in a green house. The plants were classified into four groups. Those in the first group showed morphological characters intermediate of the parentalspecies. The plants bore fruit with viable seeds. The plants showed a chromosome number of 2n=48, the sum of those of the parental species, and are suggested to be symmetric fusion products. While plants in the other groupswas less vigorous and showed chromosome number 2n= 68 to 72 suggesting asymmetric fusion products by genomic in situ hybridization(GISH). Isozyme pattern of shikimate dehydrogenase (SKDH; EC 1.1.1.25), isocitrate dehydrogenase (IDH; EC 1.1.1.41) and phosphoglucomutase (PGM; EC 2.7.5.1) showed that 24 regenerated plants in three groups were somatic hybrids. Analysis of random amplified polymorphic DNA (RAPD) showed that 43 S. integrifolium-specific and 57 S. sanitwongsei-specific bands were all found in 24 plants. Both somatic hybrids and its S1 plants were found to be resistant to bacterial wilt, and eggplant grafted these plants using for rootstocks were more productive than grafted mother plants. Now, S1 progenies are used for commercial eggplant production in Osaka Prefecture.

• The Korean Journal of Zoology
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• v.22 no.4
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• pp.153-164
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• 1979

A total of 352 specimens of congeneric species of Agkistrodon was collected and morphometric analysis and starch-gel electrophoresis were carried out in order to investigate the taxonomic status of this genus. The results obtained in this study are as follows: Morphometric analysis 1. Three species are recognized based on Gloyd's criteria. There was no specimen that was doubtful to classify correctly. Therefore, it seems that Gloyd's morphological characters are good criteria to identify each species. 2. All three species are sympatric at two localities (Mt. Seolag, Gangwon-do, and Mt. Deogyu, Jeonra-bugdo) and A. caligino년 and A. b. brevicaudus are sympatric at Gwangneung and Mt. Yongmun, Gyonggi-do. No hybrids were found in these sympatric localities. 3. Notable sexual dimorphism was found in meristic characters. 4. A. saxatilis has signicantly more number of ventrals and shorter tail ratio than other two species. 5. There were no significant meristic character differences between A. caligino년 and A. b. brevicaudus. Genetic analysis 1. Among 26 loci investigated, 12 loci (46.1%) were identical in their mobility, 4 loci (15.4%) were nearly identical with minor frequency differences, and 10 loci (38.5%) showed interspecific mobility difference. 2. The average proportion of polymorphic loci was 9.03%. This is considerably less than that of other vertebrates. 3. The average S and D values between species are .695 and .342 respectively. These values indicate that three taxa are distinct species. 4. A. caliginosus is genetically more related to A. saxatilis than to Agkistrodon, namely A. b. brevicaudus, A. caligino년 and A. saxatilis, as proposed by Gloyd. Far from being mere morphological variants, as suggested by Kang and Yoon (1975), they are highly distinctive evolutionary units.

• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.378-391
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• 2016

Eighteen morphological characters from 40 populations and ten isozyme loci from 35 populations of Sedum latiovalifolium and related species were examined to investigate the degree of morphological and genetic variation. The high-frequency marker alleles $MDH-2^a$ and $PGI-1^a$ in S. aizoon, S. kamtschaticum, and S. zokuriense did not appear in the populations of S. latiovalifolium. In addition, the high-frequency allele ($MDH-2^c$) in S. latiovalifolium appeared at a very low frequency in other subg. aizoon species. Thus, the isozyme data did not support a hybrid origin of S. latiovalifolium from S. aizoon with S. kamtschaticum. The results of an unweighted pair-group method using the arithmetic average method and a principal components analysis using morphological data also did not support a hybrid origin of S. latiovalifolium. However, our data strongly suggest that some individuals from the populations found in the Gumdaebong area were most likely hybrids due to introgression between S. latiovalifolium and S. kamtschaticum or S. aizoon and S. kamtschaticum.

• Korean Journal of Plant Taxonomy
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• v.35 no.1
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• pp.57-80
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• 2005

Morphological variation of Quercus L. subgen. Quercus in Korea was examined by numerical methods including principal components analysis of major morphological characters. Principal components analysis revealed the presence of five major species groups of the subgen. Quercus in Korea; these include 1) a group consisiting of Q. acutissima and Q. variabilis individuals, 2) Q. dentata, 3) Q. aliena, 4) Q. mongolica, and 5) Q. serrata. The putative interspecific hybrid individuals occupied intermediate position between the putative parent species in the PCA plot. The analysis also strongly suggested that size and shape of leaf blade and teeth or lobes, twig diameter, size and shape of nuts, and length and width of scales are very useful in distinguishing the species and the putative hybrids of the subgen. Quercus.

• Korean journal of applied entomology
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• v.35 no.3
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• pp.216-220
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• 1996

Drosophila simulans and D. mauritiana are sibling species, the former cosmopolitan and the latter restricted to the oceanic island of Mauritius. Sex comb-tooth number of male flies of D. simulans were about 9.83, while those of D. mauritiana were 12.90. Genital arch of D. simulans is large semicircular shaped expasion, while that of D. mauritiana is a narrow fingerlike expansion. We used classical genetic analysis to measure effects of genes on the X chromosome responsible for numeral and morphological differences in sex comb-tooth and genital arch between these species, respectively. For these purposes, mutant strain of D. simulans and wild type strain of D. mauritiana were hybridized and males of the FI and the backcrossed progenies were compared with two characters above mentioned. The sex comb-tooth number of F, males were about 11.79, and the genitalia of F, male were intermediate in shape between those of D. simulans and D. mauritiana. Genetic analysis of sex comb-tooth number and genital arches differing between D. simulans and D. mauritiana showed that very little diffemce was due to effect of the X chromosome.

• Journal of Mushroom
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• v.11 no.1
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• pp.1-8
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• 2013

In this study, twelve of Lepista nuda were collected from various localities in Korea. Also thirteen exotic L. nuda species were collected from Japan, France, Switzerland and Portugal. Spores were isolated under optical microscope. These spores were placed on the surface of YM medium for inducing to germination. Eleven mating-groups were selected by morphological characters of fruit body such as size, color and stipe patterns. Intra-isolate crosses were made between two single-spore isolates derived from mating-groups. Also, dikaryotic crossing using the isolates from L. nuda were carried out to evaluated tetrakaryon formation. Cross-mating compatibility tests also verified its dikaryotic state by microscopic or molecular genetic observation of clamp connection and Random Amplified Polymorphic DNA (RAPD) band pattern. To analyze the growth rate of hybrids and parents mycelium in dikaryons obtained from compatible mating groups were placed on PDA medium. Intra-isolate crosses determined eleven mating-groups within L. nuda. The typical clamp connection were mostly observed in mating-groups of Korean L. nuda in $K1{\times}K2$, $K1{\times}K3$, $K1{\times}K4$, $K1{\times}K6$, $K1{\times}K5$, $K2{\times}K4$, $K2{\times}K3$, $K2{\times}K6$, $K3{\times}K4$, $K4{\times}K5$, and $K4{\times}K6$. Korean L. nuda type of dikaryon, shown to cross-incompatibility with L. sordida, it seemed that mating induce more rapidly than wild types in a view of growth rate. In conclusion, it would be useful to improve mass production with better morphological characteristics through a special mating of L. nuda.

• Journal of Sericultural and Entomological Science
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• v.22 no.1
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• pp.26-45
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• 1980

Silk protein is synthesized in the silkgland of silkworm, Bombyx mori L. It is evident that silk productivity is one of the high heritable characters from the genetical aspects. It is also changed with the environmental circumstances. With this regard, this paper dealt with the varietal patterns of silkgland development and the factors concerning to the silk productivity of silkgland of silkworm by the synthesis of nucleic acids, profiles of amino acids and histological basis, using the eight parent silkworm varieties and their F$_1$ hybrids. 1. The weight of silkgland per larva increased proportionally in the F$_1$ hybrids which were crossed between longer silk yielding varieties. The silk content to the weight of the silkgland was higher in the longer silk yielding varieties than that in the shorter silk yielding varieties. 2. It was observed that the morphological changes of nuclei took place in the posterior silkgland cells with the larval development of the 5th instar. In varietal aspect, Jam 107 and Jam 108, longer silk yielding varieties, showed more branches in nuclei than the $N_2$ and $C_{60}$ which were shorter yielding ones. 3. It was observed that there was a high correlation between RNA content per unit weight of silkgland on the 6th day stage of 5th instar and silk productivity both in the parents and their F$_1$ hybrids. 4. RNA and DNA synthesis brought about thirty percent increase in the posterior silkgland of the longer silk yielding varieties during the 2nd day to the 4th day stages of the 5th instar, when compared with those in the posterior silkgland of the shorter silk yielding varieties. 5. RNA/DNA ratio in the posterior silkgland on the 2nd day and 4th day stages of the 5th instar was more increased in the longer silk yielding varieties than the shorter silk yielding varieties. 6. It was shown that DNA content for the longer silk yielding varieties came to be 374$\mu\textrm{g}$ per larva in the posterior division of silkgland on the 4th day stage of 5th instar, whereas it was 199$\mu\textrm{g}$ per larva for the shorter silk yielding varieties. 7 There was 34.8％ Alanine, 22.8％ Glycine, 9.1％ Serine and 7.3％ Tyrosine in the posterior division of silkgland as major amino acids. It is noticed that there was a little differences between the amino acids composition of posterior silkgland and silk fibroin. 8. There was some differences in the amino acids composition of posterior silkgland between pure lines and their hybrids. Glycine, Serine and acidic amino acids, essential to silk formation, seemed to be increased in the F$_1$ hybrids, whereas other amino acids such as Valine, Iso-leucine, Leucine, Lysine. Phenylalanine, Histidine and Arginine were reduced. 9. The content of Glycine, Alanine and Serine in the posterior division of silkgland was elevated in the longer silk yielding varieties than the others. Consequently. these three amino acids in the posterior silkgland seemed to be related to the silk yielding ability in the longer silk yielding varieties.s.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.207-214
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• 1996

This study was conducted to obtain fundamental information on forage sorghum breeding in forage crop field of Livestock Experiment Station at Suwon from 1986 to 1991. The charcterization of sorghum germplasm was performed through 1986 to 1987, and after parental lines were selected from diverse sorghum germplasm on the basis of flowering date, plant height and several morphological characters for forage sorghum Fl hybrids. The range of variation of 50% flowering date and plant height were greater in order of forage sorghum sudangrass and male sterile line of grain sorghum. The average flowering date was earlier in sudangrass and male sterile line of grain sorghum than forage sorghum lines from the tested sorghum germplasms. And the average plant height was tall in order of forage sorghum, sudangrass and male sterile lines of grain sorghum. There were remarkable morphological variations between sudangrass lines and male sterile lines of grain sorghum such as plant color, leaf midrib color, glume color, seed coat color, head compactness and shape, awns, grain covering and 100 seed weight.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.328-333
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• 1984

Variation in ploidy level of regenerated plants from rice anthers and effective diploidization methods of haploid plants were studied to obtain basic information in rice breeding through anther culture. In a total of 574 plants derived from anther culture using 14F$_1$ hybrids as materials, there were 49.7% haploids, 48.6% diploids and 1.7% polyploids, respectively. The frequency of haploids in Japonica/Indica crosses was 60.6%, and that of Japonica/Japonica crosses was 43.0% in average. Inclusion of 2.4-D or NAA as phytohormone may increase the frequency of haploids, but kinetin may increase the frequency of diploids. The rate of auto-diploidization by tiller separation of haploid plants showed 8.2% in average. The rate of diploidization by leaf-sheath injection of colchicine showed 18.8% in average. Morphological characters of haploids plants showed that 64.6% in culm length, 63.4% in panicle length, 68% in flag leaf length, and 74.4% in flag leaf width compared to diploid plants. These apparent morphological differences will contribute to identify the ploidy of plants derived from rice anther culture.