• 제목/요약/키워드: morphological analysis

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Morphological and molecular identification of Alaria paradisea (Phaeophyceae, Laminariales) from the Kurile Islands

  • Klimova, Anna V.;Klochkova, Nina G.;Klochkova, Tatyana A.;Kim, Gwang Hoon
    • ALGAE
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    • 제33권1호
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    • pp.37-48
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    • 2018
  • Alaria is the second largest genus of the Laminariales, which is distributed far into the northern Pacific and Atlantic oceans. Due to its high morphological plasticity, over 100 specific and sub-specific names have been used in Alaria, this has been tailored down to the present 17 species through morphological revision and molecular phylogenetic analysis. Endemic species of Alaria from Russian Far East have not been thoroughly revised since their original description, and few of them were confirmed using molecular data until recently. We carried out morphological and molecular studies on A. paradisea which is an endemic species distributed on the Kurile Islands, first described by Miyabe and Nagai in 1932 as Pleuropterum paradiseum. The range of morphological variability and its distribution was re-evaluated using the type specimen as well as other specimens. Analyses of partial mitochondrial cytochrome c oxidase subunit 1 and nuclear-encoded internal transcribed spacer sequences showed that A. paradisea nested within the genus Alaria, but differs morphologically from any other Alaria species in having additional sporophylls with a central midrib (${\beta}-sporophylls$). Our results showed that A. paradisea clearly belongs to the genus Alaria based on DNA data, although the key morphological character that was used to include this species to the genus Pleuropterum, ${\beta}-sporophylls$, is stable and distinguishes it from other Alaria species.

Morphological remodeling in mouse vagina due to hormonal hypersecretion

  • Oh, Min-Gee;Kim, Sang-Hwan
    • 한국동물생명공학회지
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    • 제37권1호
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    • pp.42-47
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    • 2022
  • This study aimed to determine whether hormonal hypersecretion could cause morphological problems in the mouse vagina and affect the ovaries and nearby extra uterine organs. All mice were synchronized to estrus before the experiment. Then human chorionic gonadotropin (hCG), progesterone, and testosterone were continuously administered for about 6 days to maintain hormone hypersecretion, and then morphological changes were analyzed, and Matrix metalloproteinases (MMPs) activity and Casp-3 expression were evaluated. As a result of the analysis, in the case of hCG, the morphological change did not show a significant difference from the vagina of normal estrus. In the case of progesterone, changes were observed in the mucosa zone and basal membrane, and it was confirmed that the activity of MMPs was increased in squamous epithelium cells. On the other hand, in the case of testosterone, overall changes in vaginal tissues were observed, and MMPs activity was increased to a very high level in all sections. The expression of Casp-3 was also the highest compared to other groups. Therefore, as a result of this study, it is thought that hormone hypersecretion affects the morphological changes of the vagina other than the ovaries and uterus and induces the activity of MMPs to cause morphological degeneration of tissues.

국내 자생 꾸지뽕나무 잎과 열매의 형태적 특성에 관한 연구 (A Study on the Morphological Characteristics of Leaves and Fruit of Cudrania tricuspidata in Korea)

  • 권용석;박보람;이솔;유한춘;백소진;오찬진
    • 한국자원식물학회지
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    • 제27권4호
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    • pp.337-343
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    • 2014
  • 꾸지뽕나무의 수요 증가에 따라 자생지 파괴가 심각한 반면 꾸지뽕나무에 대한 연구는 미비하기 때문에 자생지 복원과 새로운 농가 소득원으로 이용하기 위하여 국내에 자생하는 꾸지뽕나무 16개 집단을 대상으로 엽과 열매의 형태적 특성을 분석한 결과는 다음과 같다. 조사된 꾸지뽕나무 집단에서 엽의 형태적 특성에 대한 유집분석 결과 4개의 그룹으로 구분되었다. 열매의 형태적 특성을 분석하기 위해 꾸지뽕나무 15개 집단의 열매길이, 폭, 무게 등 3가지 형질을 분산 분석한 결과, 유의확률이 p=0.05로 각각의 형질에 따라 집단 간에 차이가 있는 것으로 나타났으며 열매의 3가지 형질별 특성을 이용하여 군집분석을 실시한 결과 세 집단으로 구분되었다. 본 연구결과에 따르면 꾸지뽕나무 엽과 열매의 형태학적 특징은 각 집단 간 차이가 있었으며, 엽의 형태적 특징과 열매의 특징이 일치하지는 않았다.

한국 연안에 출현하는 무각와편모조류 5종에 대한 형태 및 분자계통학적 특성 (Morphological Characteristics and Molecular Phylogeny of Five Unarmored Dinoflagellates in Korean Coastal Waters)

  • 조수연;기장서;한명수
    • ALGAE
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    • 제23권1호
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    • pp.15-29
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    • 2008
  • Many species in Gymnodiniales, which are unarmored dinoflagellates, are responsible for marine algal blooms and some of them have potent toxin in the cell. Their taxonomy has so far been well-defined, and several genera (e.g. Akashiwo, Gymnodinium, Karenia) have recently been re-described. In Korea, few works have been carried out on their taxonomical and molecular studies. This study focused on comparison of both morphological and molecular characteristics of five unarmored dinoflagellates on Korean coastal water: Akashiwo sanguinea, Cochlodinium polykrikoides, Gymnodinium catenatum, Gymnodinium impudicum and Karenia aureolum (=K. mikimotoi). Morphological characteristics observed here was in good accordance with the original descriptions of individual species. In addition, none of difference was found in morphological comparisons between the Korean and foreign strains. Furthermore, molecular analysis showed that the SSU rDNA sequences were generally identical according to each species. In some distinct features, A. sanguinea, which has generally the same morphological features, were divided into two groups: one was Korean isolates including European isolates, the other was American isolates. In the two groups, the nucleus was positioned differently: middle of the cells in the Korean isolates (GnSg02, GnSg03), near the epicone in American isolates (CCMP1593, CCMP1837). In addition, this was strongly supported by phylogenetic analysis, inferred from the SSU rDNA sequences. K. aureolum (GrAr01) was corresponded to European G. aureolum (=K. mikimotoi) in shape and position of nucleus, chloroplast, however, which is similar to K. digitata in view of having a finger-like sulcus. This was in good agreement with phylogenetic study of these species. G. catenatum have identical morphology except the ridge location, and their genotype of SSU rDNA was also identical to GenBank data of the same species. From this study, we found that the five Korean unarmored dinoflagellates are identical morphological characteristics and genotype to each species of foreign isolates.

형태학적 워터쉐드 알고리즘을 이용한 효율적인 영상분할 (Efficient Image Segmentation Using Morphological Watershed Algorithm)

  • 김영우;임재영;이원열;김세윤;임동훈
    • 응용통계연구
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    • 제22권4호
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    • pp.709-721
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    • 2009
  • 본 논문은 형태학적 워터쉐드 알고리즘을 이용하여 잡음에 강한 효율적인 영상분할에 대해서 논의하고자 한다. 기존의 형태학적 워터쉐드 알고리즘에 의한 영상분할은 크게 형태학적 연산자에 의한 영상의 단순화, 경사 영상 생성, 워터쉐드 알고리즘 수행 그리고 영역 병합 등의 여러 단계에 걸쳐 이루어진다. 그러나 기존의 형태학적 워터쉐드 알고리즘에 의한 영상분할은 과분할이 많이 일어나는 단점을 갖고 있다. 본 논문에서는 과분할을 줄이기 위해 잡음에 강한 형태학적 연산자에 의한 경사영상을 생성하고 워터쉐드 알고리즘을 적용 후 통계적인 콜모고로프-스미르노프 검정을 사용하여 인접한 영역 간의 픽셀 값 분포를 비교함으로써 부적절한 영역 병합을 최소화하였다. 본 논문에서 제안한 영상분할의 성능을 평가하기 위해 기존의 방법과 정성적이고 정량적인 비교뿐 만아니라 영상분할에 소요되는 계산시간까지 비교하였다.

Morphological differences between Water deer and Sika deer ovaries during estrus and pregnancy

  • Ji-Hye Lee;Yong-Su Park;Min-Gee Oh;Sang-Hwan Kim
    • 한국동물생명공학회지
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    • 제38권2호
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    • pp.62-69
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    • 2023
  • Background: Research on the reproductive physiology of Water and Sika deer, an endemic in Korea, still needs to be completed. This study analyzed the ovarian development and morphological characteristics of wild Water deer and Sika deer. Methods: Water deer and Sika deer ovaries were collected from the Korean Peninsula and Russia-Korean Peninsula border during the estrus and pregnancy seasons, respectively. And, morphological and physiological analysis and immunohistochemistry were conducted to confirm the detection of Ca2+ and assess the morphological changes in the ovaries. Results: The results of morphological analysis of ovaries during pregnancy and estrus, the development of the corpus luteum and follicles of Water deer showed similar patterns to other mammals. In contrast, the corpus luteum of Sika deer differed in tissue morphology and composition from Water deer. Ca2+ related to tissue metabolism was detected in the theca cells zone of Water deer on the estrus and was highly detected in the luteum cells zone during pregnancy. The hormone receptor protein expression patterns were generally higher in the ovaries of Water deer on the estrus and the pregnancy than in Sika deer. The expression of LH receptor was relatively low in the lutein cell zone, unlikely that of Water deer. The expression of VEGF was also different from Water deer, and the response in Sika deer was relatively very low compared to Water deer in expressing all proteins-related development. Conclusions: Therefore, the results of the study were shown that the composition of the corpus luteum of Sika deer is not clear compared to Water deer, and there are many differences in the functional and morphological formation of the corpus luteum.

NB 모델을 이용한 형태소 복원 (Morpheme Recovery Based on Naïve Bayes Model)

  • 김재훈;전길호
    • 정보처리학회논문지B
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    • 제19B권3호
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    • pp.195-200
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    • 2012
  • 한국어는 교착어이어서 형태소 분석 없이 품사 부착이 어려울 뿐 아니라 형태소를 분석할 때 다양한 어형 변화가 복원되어야 한다. 이것은 한국어 형태소 분석의 고질적인 문제 중 하나이며, 주로 규칙을 이용해서 해결한다. 규칙을 이용할 경우 주어진 문맥에 가장 적합한 복원을 어려워 여러 형태의 모호성을 생성하며, 이는 품사 부착에 의해서 해결된다. 본 논문에서는 이 문제를 기계학습 방법(Na$\ddot{i}$ve Bayes 모델)을 이용하여 해결한다. 기계학습 모델의 입력 자질은 어형 변화가 발생하는 주변 음절이며 출력 범주는 복원된 음절이다. ETRI 구문 말뭉치를 이용한 실험에서 제안된 형태소 복원 모델을 사용한 형태소 단위의 품사 부착 성능은 97.5%의 $F_1$점수를 보였으며 이 모델이 형태소 복원에 매우 유용함을 알 수 있었다.

한국산 녹조식물 해캄속(SPIROGYRA)의 수리표현학 (Numerical Phenetics of the Genus Spirogyra (Zygnematales, Chlorophyta) in Korea)

  • 김지환;김영환;이인규
    • ALGAE
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    • 제18권4호
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    • pp.239-253
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    • 2003
  • The morphotaxonomic characters of the genus Spirogyra were investigated to clarify their taxonomic category and the variation range among species on the basis of comparative morphological and numerical analyses by unialgal cultures and field samples collected from various freshwater habitats in Korea. 25 characters selected on morphological feature of the species were examined on 568 individuals for morphological comparisons and numerical analyses. Width, length and their length/width ratio of vegetative cell, shape of septum, chloroplast number, maximum width, width, length and their length/width ratio of female gametangium, length of male gametangium, size and shape of zygospore, and cell wall ornamentation of the spore showed a comparatively high vector in principal component analysis. In cluster analysis, 15 taxa analysed were divided into 8 major groups by the average taxonomic distance 1.0 level. Considering the morphology and numerical analysis, Spirogyra crassoidea could not be recognized as an independent species, therefore it was treated as a variety of S. ellipsospora. S. koreana (nom. invalidum), recognized as a new taxon, is under the investigation for its clear taxonomic category.

First Report of Endophytic Fungi, Aequabiliella effusa, Isolated from Twigs of Larix gmelinii var. olgensis in Korea

  • Jae-Eui Cha;Yun-Jeong Kim;Ahn-Heum Eom
    • 한국균학회지
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    • 제52권1호
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    • pp.13-18
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    • 2024
  • We isolated endophytic fungal strains from twigs of Larix gmelinii var. olgensis. These strains were identified based on their morphological characteristics and phylogenetic analyses including internal transcribed spacers and large subunit rDNA. We confirmed the presence of a previously unrecorded fungal species in Korea, Aequabiliella effusa. This study presents the morphological characteristics and phylogenetic analysis of A. effusa.