• Title/Summary/Keyword: morphological analysis

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Morphological and molecular identification of Alaria paradisea (Phaeophyceae, Laminariales) from the Kurile Islands

  • Klimova, Anna V.;Klochkova, Nina G.;Klochkova, Tatyana A.;Kim, Gwang Hoon
    • ALGAE
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    • v.33 no.1
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    • pp.37-48
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    • 2018
  • Alaria is the second largest genus of the Laminariales, which is distributed far into the northern Pacific and Atlantic oceans. Due to its high morphological plasticity, over 100 specific and sub-specific names have been used in Alaria, this has been tailored down to the present 17 species through morphological revision and molecular phylogenetic analysis. Endemic species of Alaria from Russian Far East have not been thoroughly revised since their original description, and few of them were confirmed using molecular data until recently. We carried out morphological and molecular studies on A. paradisea which is an endemic species distributed on the Kurile Islands, first described by Miyabe and Nagai in 1932 as Pleuropterum paradiseum. The range of morphological variability and its distribution was re-evaluated using the type specimen as well as other specimens. Analyses of partial mitochondrial cytochrome c oxidase subunit 1 and nuclear-encoded internal transcribed spacer sequences showed that A. paradisea nested within the genus Alaria, but differs morphologically from any other Alaria species in having additional sporophylls with a central midrib (${\beta}-sporophylls$). Our results showed that A. paradisea clearly belongs to the genus Alaria based on DNA data, although the key morphological character that was used to include this species to the genus Pleuropterum, ${\beta}-sporophylls$, is stable and distinguishes it from other Alaria species.

Morphological remodeling in mouse vagina due to hormonal hypersecretion

  • Oh, Min-Gee;Kim, Sang-Hwan
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.1
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    • pp.42-47
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    • 2022
  • This study aimed to determine whether hormonal hypersecretion could cause morphological problems in the mouse vagina and affect the ovaries and nearby extra uterine organs. All mice were synchronized to estrus before the experiment. Then human chorionic gonadotropin (hCG), progesterone, and testosterone were continuously administered for about 6 days to maintain hormone hypersecretion, and then morphological changes were analyzed, and Matrix metalloproteinases (MMPs) activity and Casp-3 expression were evaluated. As a result of the analysis, in the case of hCG, the morphological change did not show a significant difference from the vagina of normal estrus. In the case of progesterone, changes were observed in the mucosa zone and basal membrane, and it was confirmed that the activity of MMPs was increased in squamous epithelium cells. On the other hand, in the case of testosterone, overall changes in vaginal tissues were observed, and MMPs activity was increased to a very high level in all sections. The expression of Casp-3 was also the highest compared to other groups. Therefore, as a result of this study, it is thought that hormone hypersecretion affects the morphological changes of the vagina other than the ovaries and uterus and induces the activity of MMPs to cause morphological degeneration of tissues.

A Study on the Morphological Characteristics of Leaves and Fruit of Cudrania tricuspidata in Korea (국내 자생 꾸지뽕나무 잎과 열매의 형태적 특성에 관한 연구)

  • Kwon, Yong-Seok;Park, Bo-Ram;Lee, Sol;Yu2, Han-Chun;Baek, So-Jin;Oh, Chan-Jin
    • Korean Journal of Plant Resources
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    • v.27 no.4
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    • pp.337-343
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    • 2014
  • This study was conducted to classify the variation in leaf and fruit morphology among natural grown 16 Cudrania tricuspidata stands. As results of the analysis in C. tricuspidata stands total 134 Species were found and as results of the analysis in leaf morphology, it was grouped as Group IV. By the analysis of variance in length, width, and weight of fruits were differed in 15 C. tricuspidata stands. The length, width, and weight of fruits were higher in Jinan and Sunchang, than Gochang, Gimje, and Goheung. By the cluster analysis of 3 fruit morphology were classified as 3 Groups. According to the results of this study C. tricuspidata leaves and fruit morphological characteristics for each group were compared by analysis there were differences between the morphological characteristics and the characteristics of the fruit did not match.

Morphological Characteristics and Molecular Phylogeny of Five Unarmored Dinoflagellates in Korean Coastal Waters (한국 연안에 출현하는 무각와편모조류 5종에 대한 형태 및 분자계통학적 특성)

  • Cho, Soo-Yeon;Ki, Jang-Seu;Han, Myung-Soo
    • ALGAE
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    • v.23 no.1
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    • pp.15-29
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    • 2008
  • Many species in Gymnodiniales, which are unarmored dinoflagellates, are responsible for marine algal blooms and some of them have potent toxin in the cell. Their taxonomy has so far been well-defined, and several genera (e.g. Akashiwo, Gymnodinium, Karenia) have recently been re-described. In Korea, few works have been carried out on their taxonomical and molecular studies. This study focused on comparison of both morphological and molecular characteristics of five unarmored dinoflagellates on Korean coastal water: Akashiwo sanguinea, Cochlodinium polykrikoides, Gymnodinium catenatum, Gymnodinium impudicum and Karenia aureolum (=K. mikimotoi). Morphological characteristics observed here was in good accordance with the original descriptions of individual species. In addition, none of difference was found in morphological comparisons between the Korean and foreign strains. Furthermore, molecular analysis showed that the SSU rDNA sequences were generally identical according to each species. In some distinct features, A. sanguinea, which has generally the same morphological features, were divided into two groups: one was Korean isolates including European isolates, the other was American isolates. In the two groups, the nucleus was positioned differently: middle of the cells in the Korean isolates (GnSg02, GnSg03), near the epicone in American isolates (CCMP1593, CCMP1837). In addition, this was strongly supported by phylogenetic analysis, inferred from the SSU rDNA sequences. K. aureolum (GrAr01) was corresponded to European G. aureolum (=K. mikimotoi) in shape and position of nucleus, chloroplast, however, which is similar to K. digitata in view of having a finger-like sulcus. This was in good agreement with phylogenetic study of these species. G. catenatum have identical morphology except the ridge location, and their genotype of SSU rDNA was also identical to GenBank data of the same species. From this study, we found that the five Korean unarmored dinoflagellates are identical morphological characteristics and genotype to each species of foreign isolates.

Efficient Image Segmentation Using Morphological Watershed Algorithm (형태학적 워터쉐드 알고리즘을 이용한 효율적인 영상분할)

  • Kim, Young-Woo;Lim, Jae-Young;Lee, Won-Yeol;Kim, Se-Yun;Lim, Dong-Hoon
    • The Korean Journal of Applied Statistics
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    • v.22 no.4
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    • pp.709-721
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    • 2009
  • This paper discusses an efficient image segmentation using morphological watershed algorithm that is robust to noise. Morphological image segmentation consists of four steps: image simplification, computation of gradient image and watershed algorithm and region merging. Conventional watershed segmentation exhibits a serious weakness for over-segmentation of images. In this paper we present a morphological edge detection methods for detecting edges under noisy condition and apply our watershed algorithm to the resulting gradient images and merge regions using Kolmogorov-Smirnov test for eliminating irrelevant regions in the resulting segmented images. Experimental results are analyzed in both qualitative analysis through visual inspection and quantitative analysis with percentage error as well as computational time needed to segment images. The proposed algorithm can efficiently improve segmentation accuracy and significantly reduce the speed of computational time.

Morphological differences between Water deer and Sika deer ovaries during estrus and pregnancy

  • Ji-Hye Lee;Yong-Su Park;Min-Gee Oh;Sang-Hwan Kim
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.2
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    • pp.62-69
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    • 2023
  • Background: Research on the reproductive physiology of Water and Sika deer, an endemic in Korea, still needs to be completed. This study analyzed the ovarian development and morphological characteristics of wild Water deer and Sika deer. Methods: Water deer and Sika deer ovaries were collected from the Korean Peninsula and Russia-Korean Peninsula border during the estrus and pregnancy seasons, respectively. And, morphological and physiological analysis and immunohistochemistry were conducted to confirm the detection of Ca2+ and assess the morphological changes in the ovaries. Results: The results of morphological analysis of ovaries during pregnancy and estrus, the development of the corpus luteum and follicles of Water deer showed similar patterns to other mammals. In contrast, the corpus luteum of Sika deer differed in tissue morphology and composition from Water deer. Ca2+ related to tissue metabolism was detected in the theca cells zone of Water deer on the estrus and was highly detected in the luteum cells zone during pregnancy. The hormone receptor protein expression patterns were generally higher in the ovaries of Water deer on the estrus and the pregnancy than in Sika deer. The expression of LH receptor was relatively low in the lutein cell zone, unlikely that of Water deer. The expression of VEGF was also different from Water deer, and the response in Sika deer was relatively very low compared to Water deer in expressing all proteins-related development. Conclusions: Therefore, the results of the study were shown that the composition of the corpus luteum of Sika deer is not clear compared to Water deer, and there are many differences in the functional and morphological formation of the corpus luteum.

Morpheme Recovery Based on Naïve Bayes Model (NB 모델을 이용한 형태소 복원)

  • Kim, Jae-Hoon;Jeon, Kil-Ho
    • The KIPS Transactions:PartB
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    • v.19B no.3
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    • pp.195-200
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    • 2012
  • In Korean, spelling change in various forms must be recovered into base forms in morphological analysis as well as part-of-speech (POS) tagging is difficult without morphological analysis because Korean is agglutinative. This is one of notorious problems in Korean morphological analysis and has been solved by morpheme recovery rules, which generate morphological ambiguity resolved by POS tagging. In this paper, we propose a morpheme recovery scheme based on machine learning methods like Na$\ddot{i}$ve Bayes models. Input features of the models are the surrounding context of the syllable which the spelling change is occurred and categories of the models are the recovered syllables. The POS tagging system with the proposed model has demonstrated the $F_1$-score of 97.5% for the ETRI tree-tagged corpus. Thus it can be decided that the proposed model is very useful to handle morpheme recovery in Korean.

Numerical Phenetics of the Genus Spirogyra (Zygnematales, Chlorophyta) in Korea (한국산 녹조식물 해캄속(SPIROGYRA)의 수리표현학)

  • Kim, Ji-Hwan;Kim, Yeong-Hwan;Lee, In-Gyu
    • ALGAE
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    • v.18 no.4
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    • pp.239-253
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    • 2003
  • The morphotaxonomic characters of the genus Spirogyra were investigated to clarify their taxonomic category and the variation range among species on the basis of comparative morphological and numerical analyses by unialgal cultures and field samples collected from various freshwater habitats in Korea. 25 characters selected on morphological feature of the species were examined on 568 individuals for morphological comparisons and numerical analyses. Width, length and their length/width ratio of vegetative cell, shape of septum, chloroplast number, maximum width, width, length and their length/width ratio of female gametangium, length of male gametangium, size and shape of zygospore, and cell wall ornamentation of the spore showed a comparatively high vector in principal component analysis. In cluster analysis, 15 taxa analysed were divided into 8 major groups by the average taxonomic distance 1.0 level. Considering the morphology and numerical analysis, Spirogyra crassoidea could not be recognized as an independent species, therefore it was treated as a variety of S. ellipsospora. S. koreana (nom. invalidum), recognized as a new taxon, is under the investigation for its clear taxonomic category.

First Report of Endophytic Fungi, Aequabiliella effusa, Isolated from Twigs of Larix gmelinii var. olgensis in Korea

  • Jae-Eui Cha;Yun-Jeong Kim;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.52 no.1
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    • pp.13-18
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    • 2024
  • We isolated endophytic fungal strains from twigs of Larix gmelinii var. olgensis. These strains were identified based on their morphological characteristics and phylogenetic analyses including internal transcribed spacers and large subunit rDNA. We confirmed the presence of a previously unrecorded fungal species in Korea, Aequabiliella effusa. This study presents the morphological characteristics and phylogenetic analysis of A. effusa.