• Title/Summary/Keyword: molecular variation

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In Vivo Reporter Gene Imaging: Recent Progress of PET and Optical Imaging Approaches

  • Min, Jung-Joon
    • Bioinformatics and Biosystems
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    • v.1 no.1
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    • pp.17-27
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    • 2006
  • Recent progress in the development of non-invasive imaging technologies continues to strengthen the role of molecular imaging biological research. These tools have been validated recently in variety of research models, and have been shown to provide continuous quantitative monitoring of the location(s), magnitude, and time-variation of gene delivery and/or expression. This article reviews the use of radionuclide, magnetic resonance, and optical imaging technologies as they have been used in imaging gene delivery and gene expression for molecular imaging applications. The studies published to date demonstrate that noninvasive imaging tools will help to accelerate pre-clinical model validation as well as allow for clinical monitoring of human diseases.

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First Hyperpolarizabilities of Heteroaromatic Stilbene Derivatives

  • Cho, Bong-Rae;Lee, Sang-Hae;Yosep Min;Kang, Tae-Im;Jeon, Seung-Joon
    • Journal of Photoscience
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    • v.8 no.2
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    • pp.79-82
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    • 2001
  • 2-(p-Diethylaminostyryl)pyrrole (I) and 2-[5-diethylaminothienyl]vinyl]pyrrole (II) derivatives with systematic variation of the acceptors have been synthesized and their first hyperpolarizabilities were measured. The $\beta$ values increased systematically as the aromatic resonance energy decreased. Moreover, the value of $\beta$ for the former increased gradually as the acceptor strength increased. The opposite trend observed in the latter series of compounds has been attributed to the distorted structure caused by the steric hindrance between the N-methyl group and the acceptor moiety.

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Cyanobacterial Taxonomy: Current Problems and Prospects for the Integration of Traditional and Molecular Approaches

  • Komarek, Jiri
    • ALGAE
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    • v.21 no.4
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    • pp.349-375
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    • 2006
  • The application of modern ecological, ultrastructural and molecular methods, aided by the cultivation of numerous cyanobacterial morphotypes, has substantially changed our knowledge of these organisms. It has led to major advances in cyanobacterial taxonomy and criteria for their phylogenetic classification. Molecular data provide basic criteria for cyanobacterial taxonomy; however, a correct phylogenetic system cannot be constructed without combining genetic data with knowledge from the previous 150 years research of cyanobacterial diversity. Thus, studies of morphological variation in nature, and modern morphological, ultrastructural, ecophysiological and biochemical characters need to be combined in a “polyphasic” approach. Taxonomic concepts for generic and infrageneric ranks are re-evaluated in light of combined phenotypic and molecular criteria. Despite their usefulness in experimental studies, the limitations of using strains from culture collections for systematic and nomenclatural purposes is highlighted. The need for a continual revision of strain identification and proper nomenclatural practice associated with either the bacteriological or botanical codes is emphasized. Recent advances in taxonomy are highlighted in the context of prospects for understanding cyanobacterial diversity from natural habitats, and the evolutionary and adaptational processes that cyanobacteria undergo.

Estimation of Genetic Variation of Korean Isolates of Phytophthora capsici by Using Molecular Markers

  • Chee, Hee-Youn;Jee, Hyeong-Jin
    • Mycobiology
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    • v.29 no.1
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    • pp.43-47
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    • 2001
  • Genetic diversity of 21 Korean Phytophthora capsici isolates was analyzed by using several molecular markers such as random amplified polymorphic DNA(RAPD), M-13, microsatellite and random amplified microsatellite sequences(RAMS). The overall average similarity coefficient among the isolates was 86% based on the combined data obtained by the molecular markers. No molecular markers were found to be associated with hosts or geographic regions. In addition to RAPD, analysis based on repeated sequences such as $(GTG)_5$, M-13 and RAMS could be used to assess population structure of P. capsici.

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Monitoring Gene Therapy by Radionuclide Approaches (핵의학적 기법을 이용한 유전자 치료 영상법)

  • Min, Jung-Joon
    • Nuclear Medicine and Molecular Imaging
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    • v.40 no.2
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    • pp.96-105
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    • 2006
  • Molecular imaging has its root in nuclear medicine and gene therapy monitoring. Therefore, recent progress in the development of non-invasive imaging technologies, particularly nuclear medicine, should allow molecular imaging to play a major role in the field of gene therapy. These tools have recently been validated in gene therapy models for continuous quantitative monitoring of the location, magnitude, and time-variation of gene delivery and/or expression. This article reviews the use of radionuclide imaging technologies as they have been used in imaging gene delivery and gene expression for gene therapy applications. The studios published to date lend support that noninvasive imaging tools will help to accelerate pre-clinical model validation as well as allow for clinical monitoring of human gene therapy.

The Variation of Offset Ink Properties According to the Vegetable Fatty Acid Esters (Vegetable Fatty Acid Esters에 따른 평판 잉크의 물성 변화에 관한 연구)

  • Park, Jung-Min;Kim, Sung-Bin
    • Journal of the Korean Graphic Arts Communication Society
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    • v.29 no.3
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    • pp.1-16
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    • 2011
  • In these days, according to increased environmental regulations, to reduce the VOC content in paints and inks efforts are now in progress. A lot of research have progressed hydrocarbon solvents substitute with vegetable oil or ester in the printing ink but it is restricted to use vegetable oil in the inks because of high molecular weight and viscosity. Oil ester currently developed for printing inks still have some drawbacks, but overall the printability and print quality when using ester than hydrocarbon solvents are showing good results. Thus, in this paper, I studied about the properties variation of the varnish and inks according to vegetable ester, after I reacted vegetable ester using the vegetable fatty acid and ethyl alcohol, butyl alcohol. The compared in order of average molecular weight by the GPC method, rheological properties were found by rotational rheometer, and emulsion behavior were compared by high speed emulsification tester.

Genetic Characterization based on a rDNA Spacer, ITS2 and mtDNA, mtCOI Gene Sequences of Korean Venus Clam, Ruditapes philippinarum

  • Park, Gab-Man;Chung, Ee-Yung;Hur, Sung-Bum
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.497-498
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    • 2000
  • The venus clam, Ruditapes philippinarum, is an aquaculture shellfish mainly distributed in an intertidal zone of East Asia including Korea, China and Japan. The morphological variation of this species is great. In fact, two of the most popular markers used in molecular evolution, mitochondrial DNA (mtDNA) and nuclear ribosomal DNA (rDNA), have quite different properties, which could translate into different consequences of mutation, drift, migration and selection on patterns of geographical variation and molecular divergence. (omitted)

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Real-time X-ray Scattering as a Nanostructure Probe for Organic Photovoltaic Thin Films

  • Lee, Hyeon-Hwi;Kim, Hyo-Jeong;Kim, Jang-Ju
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.181-181
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    • 2013
  • Recently, nanostructure and the molecular orientation of organic thin films have been largely paid attention due to its importance in organic electronics such as organic thin film transistors (OTFTs), organic light emitting diodes (OLEDs), and organic photovoltaics (OPVs). Among various methods, the diffraction and scattering techniques based on synchrotron x-rays have shown powerful results in organic thin film systems. In this work, we introduce the in-situ annealing system installed at PLS-II (Pohang Light Source II) for organic thin films by simultaneously conducting various x-ray scattering measurements of x-ray reflectivity, conventional x-ray scattering, grazing incidence wide angle x-ray scattering (GI-WAXS) and so on. Using the in-situ measurement, we could obtain real time variation of nanostructure as well as molecular orientation during thermal annealing in metal-phthalocyanine thin films. The variation of surface and interface also could be simultaneously investigated by the x-ray reflectivity measurement.

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Precise Measurement of Optical Anisotropy of Rubbed Polyimide on Patterned Glass and its Nanoscale Variation (패턴이 있는 유리기층 위 러빙된 Polyimide의 광학 이방성 미세변화 정밀 측정)

  • Kim, Ha-Rang;Kim, Sang-Youl
    • Korean Journal of Optics and Photonics
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    • v.20 no.5
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    • pp.281-287
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    • 2009
  • The optical anisotropy of rubbed PI(polyimide) film on patterned LCD glass substrate is analyzed using polarimetry. The direction of the optic axis and the magnitude of the very small retardation ($\sim$ 0.4 nm or less) is precisely measured by using a transmission ellipsometer. The variation of the optical anisotropy is presented as the curve of the optic axis versus the magnitude of the phase retardation and it is explained by using a simple optical model.

Langmuir-Blodgett Methods and Photelectronic Devices (Langmuir Blodgett법에 의한 광전자소자)

  • 신동명
    • Korean Journal of Optics and Photonics
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    • v.2 no.2
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    • pp.108-113
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    • 1991
  • This paper describes the necessity and utility of Langmuir-Blodgett (L-B) methods in developing molecular electronic devices. It also covers the application area and limitations of the methods. With L-B methods, the membrane thickness can be controlled in a range of 50 nm and 1000 nm depending on nature of the materials and layering methods. The molecular arrangement within the membrane can be altered by altering the surface pressure and nature of the layering materials. Such a variation can be altered by altering the surface pressure and nature of the layering materials. Such a variation can offer a new application of the methods to the future electronic devices. More over 2nd and 3rd nonlinearity generated in the nonsymmetric thin membrane will be used in the development of the optoelectronic devices.

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