• 대한유전성대사질환학회지
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• 제1권1호
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• pp.33-37
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• 2001

• 천문학회보
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• 제43권1호
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• pp.66.2-66.2
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• 2018

The probability distribution function of column density (N-PDF) has been used for studying the characteristics of molecular clouds. In particular, the properties of N-PDF can reveal the nature of turbulence and gravity inside the molecular cloud. We use the dust continuum emission at $450{\mu}m$ and $850{\mu}m$ observed as part of the JCMT Gould Belt Survey (GBS) (Mairs et al. 2016), the 12CO J=1-0 line observed with the 45 m telescope at Nobeyama Radio Observatory (NRO) (Shimajiri et al. 2011), 13CO, C18O and HCO+ J=1-0 observed with the 13.7 m telescope at Taeduk Radio Astronomy Observatory (TRAO), as part of the TRAO key science project, "mapping Turbulent properties In star-forming MolEcular clouds down to the Sonic scale" (TIMES; PI: Jeong-Eun Lee). We here present the N-PDFs derived from the continuum and the molecular line emission toward the Orion A molecular cloud and compare their behaviors in order to investigate the chemical and optical depth effects on the N-PDF.

• Journal of Veterinary Science
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• 제24권2호
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• pp.29.1-29.12
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• 2023

Background: Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear. Objectives: This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022. Methods: A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed to identify the genetic relationships between the strains. Results: An FPV strain named YBYJ-1 was successfully isolated. The virus diameter was approximately 20-24 nm, 50% tissue culture infectious dose = 1 × 10^-4.94/mL, which caused cytopathic effect in F81 cells. The epidemiological survey from 2021 to 2022 showed that 27 of the 80 samples were FPV-positive. Additionally, three strains positive for CPV-2c were unexpectedly found. Phylogenetic analysis showed that most of the 27 FPV strains belonged to the same group, and no mutations were found in the critical amino acids. Conclusions: A local FPV strain named YBYJ-1 was successfully isolated. There was no critical mutation in FPV in Yanji, but some cases with CPV-2c infected cats were identified.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.490-496
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• 1998

Random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 34 Botrytis cinerea isolates from nine different host plants in Korea. For RAPD analysis, 115 arbitrary decamer primers were initially screened for polymorphic major DNA bands with 11 representative B. cinerea isolates. Eleven primers that initially detected polymorphisms were tested a second time with additional 23 isolates of B. cinerea as well as one isolate of Botrytis squamosa as an outgroup. The RAPD analyses revealed that all isolates except one showed different molecular phenotypes. Dendrograms obtained from dissimilarity matrices using the unweighted paired group method of arithmetic means (UPGMA) showed the 36.4% to 90.0% similarity among all B. cinerea isolates. The B. squamosa isolate showed the least similarity to all B. cinerea isolates. The cluster analyses indicated no correlation among all the characteristics examined including molecular phenotypes, host and geographic origins, year of isolation, or pathogenicity. The RAPD data suggest that a high level of genetic variation exists among Korean populations of B. cinerea and it seems to be caused by heterokaryosis among preexisting molecular phenotypes.

• 천문학회지
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• 제38권2호
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• pp.33-41
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• 2005

We have conducted observations toward the molecular cloud associated with the H II region Sh 156 in $^{13}CO$(J = 1-0), $C^{18}O$(J = 1-0), and CS(J = 2 -1) using the TRAO 14 m telescope. Combining with existing $^{12}CO$(J = 1- 0) data of the Outer Galaxy Survey, we delineated the physical properties of the cloud. We found that there is a significant sign of interaction between the H II region and the molecular gas. We estimated the masses of the molecular cloud, using three different techniques; the most plausible mass is estimated to be $1.37 {\times} 10^5 M_{\bigodot}$, using a conversion factor of $X = 1.9 {\times} 10^{20}\;cm^{-2} (K\;km\;s^{-1})^{-1}$, and this is similar to virial mass estimate. This implies that the cloud is gravitationally bound and in virial equilibrium even though it is closely associated with the H II region. In addition to existing outflow, we found several MSX and IRAS point sources associated with dense core regions. Thus, more star forming activities other than the existing H II region are also going on in this region.

• Parasites, Hosts and Diseases
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• 제51권1호
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• pp.1-8
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• 2013

Taenia solium, T. saginata, and T. asiatica are taeniid tapeworms that cause taeniasis in humans and cysticercosis in intermediate host animals. Taeniases remain an important public health concerns in the world. Molecular diagnostic methods using PCR assays have been developed for rapid and accurate detection of human infecting taeniid tapeworms, including the use of sequence-specific DNA probes, PCR-RFLP, and multiplex PCR. More recently, DNA diagnosis using PCR based on histopathological specimens such as 10% formalin-fixed paraffin-embedded and stained sections mounted on slides has been applied to cestode infections. The mitochondrial gene sequence is believed to be a very useful molecular marker for not only studying evolutionary relationships among distantly related taxa, but also for investigating the phylo-biogeography of closely related species. The complete sequence of the human Taenia tapeworms mitochondrial genomes were determined, and its organization and structure were compared to other human-tropic Taenia tapeworms for which complete mitochondrial sequence data were available. The multiplex PCR assay with the Ta4978F, Ts5058F, Tso7421F, and Rev7915 primers will be useful for differential diagnosis, molecular characterization, and epidemiological surveys of human Taenia tapeworms.

• 천문학회지
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• 제39권2호
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• pp.31-40
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• 2006

Near-IR $H_2$ emission features in the northern region of the Orion A giant molecular cloud were observed in the $CO\;J\;=\;1\;{\rightarrow}\;0$ line in search of CO outflows. Out of the 30 sources surveyed, CO line wings were detected toward 28 positions, suggesting a strong correlation between $H_2$ jets and CO outflows. Blueshifted wings were detected toward 26 positions while redshifted wings were detected toward 15 positions, which suggests that there is a bias in the source selection. The bias is more severe in OMC 3 than in OMC 2. Since the protostars in OMC 3 are younger and more deeply embedded, the bias may be caused by the difference of extinction between blueshifted and redshifted outflows. Some physical parameters of the outflows were derived from the line profiles.

• 환경생물
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• 제39권2호
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• pp.236-244
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• 2021

Unarmored dinoflagellates, in the family Kareniaceae, include harmful or toxic bloom-forming species, which are associated with massive fish kills and mortalities of marine organisms worldwide. The occurrence and distribution of the toxigenic species in the family Kareniaceae were investigated in the brackish and coastal waters of Korea between July 2018 and October 2020. During the survey, we collected seven newly recorded species; Karenia papilionacea, Karlodinium digitatum, Karl. veneficum, Karl. zhouanum, Takayama acrotrocha, T. helix, and T. tasmanica. A total of fifteen strains of the seven taxa were successfully established as clonal cultures and examined using LM, SEM, and molecular phylogeny inferred from LSU rDNA sequences. Herein, we present the taxonomic information, morphological features, and molecular phylogenetic positions of the unrecorded dinoflagellate species collected from Korean coastal waters.

• 천문학논총
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• 제30권2호
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• pp.103-105
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• 2015

The 22 m diameter Mopra telescope in Australia is being used to undertake an improved survey of the CO J = 1-0 line at 3mm along the 4th quadrant of the Galaxy, achieving an order of magnitude better spatial and spectral resolution (i.e. 0.6 and 0.1 km/s) than the Dame et al. (2001) survey that is publically available for the Southern Galactic plane. Furthermore, the Mopra CO survey includes the four principal isotopologues of the CO molecule (i.e. $^{12}CO$, $^{13}CO$, $C^{18}O$ and $C^{17}O$). The survey makes use of an 8 GHz-wide spectrometer and a fast mode of on-the-fly mapping developed for the Mopra telescope, where the cycle time has been reduced to just 1/4 of a second. 38 square degrees of the Galaxy, from $l=306-344^{\circ}$, $b=0{\pm}5^{\circ}$ have currently been surveyed, together with additional 9 sq. deg. regions around the Carina complex and the Central Molecular Zone. We present new results from the survey (see also Burton et al., 2013, 2014). The Mopra CO data are being made publically available as they are published; for the latest release see the project website at www.phys.unsw.edu.au/mopraco.

• 천문학회보
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• 제34권1호
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• pp.130.1-130.1
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• 2009