• 제목/요약/키워드: molecular species

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Alsidium oliveiranum sp. nov. (Rhodomelaceae, Rhodophyta), an overlooked species from the southwestern Atlantic based on morphology and DNA sequence data

  • Guimaraes, Silvia M.P.B.;Soares, Luanda Pereira;Fujii, Mutue Toyota;Diaz-Tapia, Pilar
    • ALGAE
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    • v.34 no.3
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    • pp.187-198
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    • 2019
  • The exploration of seaweed diversity in poorly studied habitats has often led to the discovery of new species. Sand-covered rocks are an example, as they received less attention than sand-free rocky intertidal habitats during seaweed diversity surveys in Brazil. In sand-covered rocks from Espirito Santo and Rio de Janeiro we found an alga whose morphology was unique among rhodomelacean species previously reported in Brazil. With the aim to clarify the taxonomic identity of this species we studied its morphology, as well as its phylogenetic relationships. Molecular analyses resolved this species in the genus Alsidium (tribe Alsidieae) and differed from sequenced congeners with divergences ${\geq}2.5$ and 4.2% in the rbcL and cox1 genes, respectively. Morphological characters were in agreement with the genus Alsidium, and differed from other species currently recognized in the genus. The species consisted of a basal crust and scarcely branched erect axes with seven pericentral cells covered by a continuous layer of cortical cells. Reproductive structures were formed on clusters of short determinate branches. Therefore, the new species A. oliveiranum is proposed based on morphological and molecular evidence. Our findings contribute to better understand the diversity of the tribe Alsidieae, which is particularly diverse in the Americas.

New record of two marine ulvalean species (Chlorophyta) in Korea

  • Lee, Seung Hee;Kang, Pil Joon;Nam, Ki Wan
    • Journal of Ecology and Environment
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    • v.37 no.4
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    • pp.379-385
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    • 2014
  • Two marine ulvalean species (Chlorophyta) were collected from the southern and eastern coasts of Korea. One is morphologically characterized by usually unbranched or little branched ribbon-shaped thalli, fronds with spirally twisted basal portions and usually undulate margins and 1 (-2) pyrenoids per cell. The other has irregularly shaped thalli, undulate and dentate margins in the fronds, small macroscopic denticulations along the margin and (1-) 2-3 pyrenoids per cell. In phylogenetic tree based on molecular data, the two species nest in the same clade with Ulva flexuosa and U. rigida, respectively. These two Korean entities are identified as U. flexuosa and U. rigida, respectively, based on morphological and molecular analyses. This is the first record of Ulva flexuosa and U. rigida in Korea.

Analysis of Fatty Acyl Groups of Diacyl Galactolipid Molecular Species by HPLC/ESI-MS with In-source Fragmentation

  • Gil, Ji-Hye;Hong, Jong-Ki;Choe, Joong-Chul;Kim, Young-Hwan
    • Bulletin of the Korean Chemical Society
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    • v.24 no.8
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    • pp.1163-1168
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    • 2003
  • The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

Pteriomorphia (Mollusca : Bivalvia) from Ullng Island , Korea (울릉도 해안 익형아강(연체동물문: 이매패강))

  • Byung Lae Choe;Won Kim;Jong Rak Lee;Sook Hee Yoon
    • Animal Systematics, Evolution and Diversity
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    • v.10 no.1
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    • pp.61-83
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    • 1994
  • Thirty-three species or subspecies of marine peteriomorphs from Ullng Island, Korea are described . All the described species are new to the fauna of this island. Among them, five speices, Modiolus (Modiolus) comptus Sowerby, 1915, Gregareiella coralliophaga(Gmelin, 1791) , Musculus (Musculus) laevigatus (Gray, 1824), Chlamys(Coralichlamys) jousseaumei Bavay, 19.4, Spndylus (Spondylus) varius Sowerby, 1829 are newly recorded in Korean waters. These five species are redescribed.

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Monostroma alittorale, a marine green algal species newly recorded in Korea

  • An, Jae Woo;Kang, Pil Joon;Nam, Ki Wan
    • Korean Journal of Environmental Biology
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    • v.37 no.3
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    • pp.362-366
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    • 2019
  • A marine green algal species (Chlorophyta) was collected from the eastern coast of Korea. It is morphologically characterized by monostromatic thallus, usually undulate and entire margins, cap-like chloroplast and several pyrenoids per cell. In a phylogenetic tree based on molecular data, the Korean alga nests in the same clade as Monostroma alittorale originally described from Japan, as a sister clade of M. grevillei from France. The genetic distance for ITS(Internal Transcribed Spacer) sequences among Monostroma species ranges from 2.3% to 38.2%. The value between the Korean entity and M. alittorale was calculated as 0.01%, considered to be intraspecific divergence. This Korean entity is identified as Monostroma alittorale based on morphological and molecular analyses. This is the first record of M. alittorale in Korea.

Molecular Species Composition of Phosphatidylcholine Isolated from Chum Salmon Meat Oil

  • Lee, Seung-Joo;Ha, Wang-Hyun;Choi, Hye-Jin;Cho, Soon-Yeong
    • Fisheries and Aquatic Sciences
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    • v.13 no.3
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    • pp.206-209
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    • 2010
  • Chum salmon (Oncorhynchus keta) meat oil contained high amounts of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) compared to oils extracted from other tissues of the fish. EPA and DHA occupied more than 25% of the total fatty acids in chum salmon meat oil. The main lipid classes in the meat oil were triacylglycerides and phospholipids. The major fatty acids of the molecular species composition of phosphatidylcholine isolated from the meat oil were DHA and EPA. DHA and EPA were the major molecular species in the phosphatidylcholine of chum salmon meat oil, representing 44% and 17%, respectively.

Three Species of the Genus Melita from Korea (Crustacea, Amphipoda, Melitidae) (한국산 Melita속 옆새우류 (갑각상강, 단각목) 3종)

  • Kim, Chang-Bae;Kim, Won;Kim, Hoon-Soo
    • Animal Systematics, Evolution and Diversity
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    • no.nspc3
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    • pp.113-120
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    • 1992
  • A examination of the Korean specimens of Melita koreana Stephensen and Melita rylovae Bulycheva confirmed the validity of M. rylovae. These two species and Melita setiflagella Yamato, a newly recorded species from Korea, are described and illustrated.

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The Systematic Study on the Freshwater Rotifera of Korea (한국산 담수 윤형동물의 계통분류학적 연구)

  • Kim, Won;Moon, Seung-Yeo;Song, Min-Ok
    • The Korean Journal of Zoology
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    • v.34 no.4
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    • pp.548-556
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    • 1991
  • The rosters collected loom various keshwater habitats, such as rivers, steams, lakes, ponds, and rice paddies at twenty-four different sites in Korea were investigated. Eleven species and/or subspecies in three families of monogonont freshwater rotifers were identified, of which two species, Notholco marina and Lecane (Monostyra) stenroosi stenroosi were new to Korea. A key is presented for the twenty-two Korean species and/or subspecies which have been described by the previous taxonomic reports and the present study.

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Phylogenetic Analysis of the Corticiaceae Based on Gene Sequences of Nuclear 18S Ribosomal DNAs

  • Lee, Seung-Shin;Jung, Hack-Sung
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.253-258
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    • 1997
  • The nuclear 18S ribosomal RNA genes of seven corticioid species were sequenced. These sequences were analyzed and compared with those of 24 other species of the order Aphyllophorales and phylogenetic trees were constructed using parsimonious methods. Phylogenetic analyses showed that two species among examined members of the Corticiaceae, Resinicium bicolor and Thanatephorus praticola, are located distantly from the remaining six species. The separation of R. bicolor seems to be kphylogenetically significant because it has very unique cystidia. The independent lineage of T. practicola suggests that it is also phylogenetically distinct because it has unusual features like the homobasidium producing secondary spores and the spetal ultrastructure of pore cap. Furthermore, Auriscalpium vulgare, Bondarzewia berkeleyi, and Heterobasidion annosum from different families of the Aphyllophorales proved to be closely related to the species of the Corticiaceae. They all have amyloid spores and grouped with Aleyrodiscus amorphus, which is a member of the Corticiaceae. The amyloidity of spores seems to be an improtant character throughout the order of the Aphyllophorales.

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First report of Halopeltis (Rhodophyta, Rhodymeniaceae) from the non-tropical Northern Hemisphere: H. adnata (Okamura) comb. nov. from Korea, and H. pellucida sp. nov. and H. willisii sp. nov. from the North Atlantic

  • Schneider, Craig W.;Freshwater, D. Wilson;Saunders, Gary W.
    • ALGAE
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    • v.27 no.2
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    • pp.95-108
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    • 2012
  • Using genetic sequencing (COI-5P, LSU, $rbc$L) to elucidate their phylogenetic positions and then morphological characters to distinguish each from existing species, three procumbent species, including two novel species, from warm temperate Northern Hemisphere waters are added to the recently resurrected genus $Halopeltis$ J. Agardh: $H.$ $adnata$ (Okamura) comb. nov. from Korea, $H.$ $pellucida$ sp. nov. from Bermuda and $H.$ $willisii$ sp. nov. from North Carolina, USA. Prior to these reports, the genus was confined to the Southern Hemisphere and tropical equatorial waters of the Northern Hemisphere although the latter records lack molecular confirmation. These three additional species join the six known species presently residing in $Halopeltis$.