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Alsidium oliveiranum sp. nov. (Rhodomelaceae, Rhodophyta), an overlooked species from the southwestern Atlantic based on morphology and DNA sequence data  

Guimaraes, Silvia M.P.B. (Institute of Botany, Nucleus for Research in Phycology)
Soares, Luanda Pereira (Institute of Botany, Nucleus for Research in Phycology)
Fujii, Mutue Toyota (Institute of Botany, Nucleus for Research in Phycology)
Diaz-Tapia, Pilar (Coastal Biology Research Group, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of A Coruna)
Publication Information
ALGAE / v.34, no.3, 2019 , pp. 187-198 More about this Journal
The exploration of seaweed diversity in poorly studied habitats has often led to the discovery of new species. Sand-covered rocks are an example, as they received less attention than sand-free rocky intertidal habitats during seaweed diversity surveys in Brazil. In sand-covered rocks from Espirito Santo and Rio de Janeiro we found an alga whose morphology was unique among rhodomelacean species previously reported in Brazil. With the aim to clarify the taxonomic identity of this species we studied its morphology, as well as its phylogenetic relationships. Molecular analyses resolved this species in the genus Alsidium (tribe Alsidieae) and differed from sequenced congeners with divergences ${\geq}2.5$ and 4.2% in the rbcL and cox1 genes, respectively. Morphological characters were in agreement with the genus Alsidium, and differed from other species currently recognized in the genus. The species consisted of a basal crust and scarcely branched erect axes with seven pericentral cells covered by a continuous layer of cortical cells. Reproductive structures were formed on clusters of short determinate branches. Therefore, the new species A. oliveiranum is proposed based on morphological and molecular evidence. Our findings contribute to better understand the diversity of the tribe Alsidieae, which is particularly diverse in the Americas.
Alsidieae; Brazil; Ceramiales; cox1; new species; rbcL; red algae; sand-covered rocks;
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1 Diaz-Tapia, P., Maggs, C. A., Macaya, E. C. & Verbruggen, H. 2018. Widely distributed red algae often represent hidden introductions, complexes of cryptic species or species with strong phylogeographic structure. J. Phycol. 54:829-839.   DOI
2 Diaz-Tapia, P., Maggs, C. A., West, J. A. & Verbruggen, H. 2017. Analysis of chloroplast genomes and a supermatrix inform reclassification of the Rhodomelaceae (Rhodophyta). J. Phycol. 53:920-937.   DOI
3 Einav, R. 2007. Seaweeds of the eastern Mediterranean coast. A.R.G. Gantner Verlag K.G., Ruggell Press, Liechtenstein, 266 pp.
4 Falkenberg, P. 1901. Die Rhodomelaceen des Golfes von Neapel und der angrenzenden Meeres-Abschnitte. Fauna und Flora des Golfes von Neapel, Monographie 26. Koeltz Scientific Books, Berlin, 754 pp.
5 Freshwater, D. W. & Rueness, J. 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species based upon rbcL nucleotide sequence analysis. Phycologia 33:187-194.   DOI
6 Nauer, F., Cassano, V. & Oliveira, M. C. 2015. Description of Hypnea pseudomusciformis sp. nov., a new species based on molecular and morphological analyses, in the context of the H. musciformis complex (Gigartinales, Rhodophyta). J. Appl. Phycol. 27:2405-2417.   DOI
7 Norris, R. E. 1994. Some cumophytic Rhodomelaceae (Rhodophyta) occurring in Hawaiian surf. Phycologia 33:434-443.   DOI
8 Rodriguez-Prieto, C., Ballesteros, E., Boisset, F. & Afonso-Carrillo, J. 2013. Guia de las macroalgas y fanerogamas marinas del Mediterraneo occidental. Ediciones Omega, S.A., Barcelona, 656 pp.
9 Saunders, G. W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philos. Trans. R. Soc. Lond. B Biol. Sci. 360:1879-1888.   DOI
10 Garcia-Soto, G. & Lopez-Bautista, J. 2018. Taxonomic notes on the genus Alsidium C. Agardh, including the merging of Bryothamnion Kutzing (Rhodomelaceae). Algae 33:215-229.   DOI
11 Guimaraes, S. M. P. B. 2003. Uma analise da diversidade da flora marinha bentonica do estado do Espirito Santo, Brasil. Hoehnea 30:11-19.
12 Guimaraes, S. M. P. B. 2006. A revised checklist of benthic marine Rhodophyta from the State of Espirito Santo, Brazil. Bol. Inst. Bot. 17:143-194.
13 Guimaraes, S. M. P. B. & Fujii, M. T. 1998. Two species of foliose Halymenia (Halymeniaceae, Rhodophyta) from Brazil. Bot. Mar. 41:495-504.   DOI
14 Guiry, M. D. & Guiry, G. M. 2019. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Available from: Accessed Jan 5, 2019.
15 Savoie, A. M. & Saunders, G. W. 2016. A molecular phylogenetic and DNA barcode assessment of the tribe Pterosiphonieae (Ceramiales, Rhodophyta) emphasizing the Northeast Pacific. Botany 94:917-939.   DOI
16 Saunders, G. W. 2008. A DNA barcode examination of the red algal family Dumontiaceae in Canadian waters reveals substantial cryptic species diversity. 1. The foliose Dilsea-Neodilsea complex and Weeksia. Botany 86:773-789.   DOI
17 Saunders, G. W. & McDevit, D. C. 2012. Methods for DNA barcoding of photosynthetic protists emphasizing the macroalgae and diatoms. Methods Mol. Biol. 858:207-222.   DOI
18 Saunders, G. W. & Moore, T. E. 2013. Refinements for the amplification and sequencing of red algal DNA barcode and RedToL phylogenetic markers: a summary of current primers, profiles and strategies. Algae 28:31-43.   DOI
19 Schneider, C. W., Hamzeh, B. F., Lane, C. E. & Saunders, G. W. 2018. A new species of Digenea (Rhodomelaceae, Ceramiales) based upon a molecular assessment and morphological observations of plants historically known as D. simplex in Bermuda. Phytotaxa 338:90-98.   DOI
20 Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313.   DOI
21 Womersley, H. B. S. 2003. The marine benthic flora of southern Australia - Part IIID Ceramiales - Delesseriaceae, Sarcomeniaceae, Rhodomelaceae. Australian Biological Resources Study and State Herbarium of South Australia, Canberra and Adelaide, 533 pp.
22 Ximenes, C. F., Cassano, V., de Oliveira-Carvalho, M. F., Bandeira-Pedrosa, M. E., Gurgel, C. F. D., Verbruggen, H. & Pereira, S. M. B. 2017. Systematics of the genus Halimeda (Bryopsidales, Chlorophyta) in Brazil including the description of Halimeda jolyana sp. nov. Phycologia 56:369-381.   DOI
23 Boo, G. H., Robledo, D., Andrade-Sorcia, G. & Boo, S. M. 2018. Genetic discontinuity of Digenea (Rhodomelaceae, Rhodophyta) from Mexico supports recognition of two new species, D. mexicana and D. rafaelii. Algae 33:231-241.   DOI
24 Zanardini, G. 1851. Algae novae vel minus cognitae in mari Rubro a Portiero collectae. Flora 34:33-38.
25 Zuccarello, G. C., Muangmai, N., Preuss, M., Sanchez, L. B., Loiseaux de Goer, S. & West, J. A. 2015. The Bostrychia tenella species complex: morphospecies and genetic cryptic species with resurrection of B. binderi. Phycologia 54:261-270.   DOI
26 Airoldi, L. 2003. The effects of sedimentation on rocky coast assemblages. Oceanogr. Mar. Biol. Ann. Rev. 41:161-236.
27 Barbara, I., Choi, H. -G., Secilla, A., Diaz-Tapia, P., Gorostiaga, J. M., Seo, T. -K., Jung, M. -Y. & Berecibar, E. 2013. Lampisiphonia iberica gen. et sp. nov. (Ceramiales, Rhodophyta) based on morphology and molecular evidence. Phycologia 52:137-155.   DOI
28 Bompard, H. 1867. Algae novae ditionis genuensis. Hedwigia 9:129-130.
29 Iha, C., O'Shaughnessy, K. A., Guimaraes, S. M. P. B., Oliveira, M. C. & Freshwater, D. W. 2016. Taxonomic reappraisal of Gelidium coarctatum (Gelidiales, Rhodophyta) and Gelidium lineare sp. nov. from the tropical western Atlantic. Phycologia 55:555-563.   DOI
30 Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowits, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647-1649.   DOI
31 Kim, B. & Kim, M. S. 2014. Three new species of Polysiphonia sensu lato (Rhodophyta) based on the morphology and molecular evidence. Algae 29:183-195.   DOI
32 Maggs, C. A. & Hommersand, M. H. 1993. Seaweeds of the British Isles. Vol. 1. Rhodophyta. Part 3A. Ceramiales. Natural History Museum, Pelagic Publishing, London, 444 pp.
33 Kutzing, F. T. 1865. Tabulae phycologicae: oder, Abbildungen der Tange. Vol. XV. Gedruckt auf Kosten des Verfassers, Nordhausen, 36 pp.
34 Le Gall, L. & Saunders, G. W. 2010. DNA barcoding is a powerful tool to uncover red algal diversity: a case study of the Phyllophoraceae (Gigartinales, Rhodophyta) in the Canadian flora. J. Phycol. 46:374-389.   DOI
35 Littler, D. S. & Littler, M. M. 2000. Caribbean reef plants: an identification guide to the reef plants of the Caribbean, Bahamas, Florida and Gulf of Mexico. Offshore Graphics, Washington, DC, 542 pp.
36 Mamoozadeh, N. R. & Freshwater, D. W. 2011. Taxonomic notes on Caribbean Neosiphonia and Polysiphonia (Ceramiales, Florideophyceae): five species from Florida, USA and Mexico. Bot. Mar. 54:269-292.   DOI
37 Muangmai, N., von Ammon, U. & Zuccarello, G. C. 2016. Cryptic species in sympatry: nonrandom small-scale distribution patterns in Bostrychia intricata (Ceramiales, Rhodophyta). Phycologia 55:424-430.   DOI
38 Chen, D. H., Soares, L. P. & Fujii, M. T. 2019. Molecular and morphological reappraisal of Spyridiocolax capixabus (Spyridiaceae, Rhodophyta), a rare endemic parasite from Brazil. Bot. Mar. 62:345-353.   DOI
39 Brunelli, B., Jamas, M., Milstein, D., Boo, S. M. & Fujii, M. T. 2019a. Gelidium brasiliense sp. nov. (Gelidiales, Rhodophyta): a diminutive agarophyte from Brazil. J. Appl. Phycol. 31:951-958.   DOI
40 Brunelli, B., Milstein, D., Boo, S. M. & Fujii, M. T. 2019b. Gelidium guimaraesiae sp. nov. (Gelidiaceae, Rhodophyta) from the western Atlantic segregated from G. floridanum by morphological and molecular evidence. Phytotaxa 388:275-286.   DOI
41 D'Archino, R., Nelson, W., Yang, M. Y. & Kim, M. S. 2015. New record of Hypnea flexicaulis in New Zealand and description of Calliblepharis psammophilus sp. nov. Bot. Mar. 58:485-497.
42 Dawson, E. Y. 1959. Changes in Palmyra Atoll and its vegetation through the activities of man, 1913-1958. Pac. Nat. 1:3-51.
43 Dawson, E. Y. 1963. New records of marine algae from the Galapagos Islands. Pac. Nat. 4:3-23.
44 De Oliveira Filho, E. C. 1969. A new species of Calliblepharis from Brazil. Nova Hedwigia 18:769-774.
45 Diaz-Tapia, P. & Barbara, I. 2013. Seaweeds from sand-covered rocks of the Atlantic Iberian Peninsula. Part. 1. The Rhodomelaceae (Ceramiales, Rhodophyta). Cryptogam. Algol. 34:325-422.   DOI
46 Diaz-Tapia, P., Barbara, I. & Diez, I. 2013a. Multi-scale spatial variability in intertidal benthic assemblages: differences between sand-free and sand-covered rocky habitats. Estuar. Coast. Shelf Sci. 133:97-108.   DOI
47 Diaz-Tapia, P., Boo, S. M., Geraldino, P. J. L., Maneiro, I., Barbara, I. & Hommersand, M. H. 2013b. Morphology and systematics of Calliblepharis hypneoides, sp. nov. (Cystocloniaceae, Rhodophyta) from the Atlantic Iberian Peninsula. Eur. J. Phycol. 48:380-397.   DOI