• Title/Summary/Keyword: molecular monitoring

Search Result 396, Processing Time 0.028 seconds

Parylene membrane based chemomechanical explosive sensor (패럴린 박막을 이용한 기계화학적 폭발물 센서)

  • Shin, Jae-Ha;Lee, Sung-Jun;Cha, Mi-Sun;Kim, Mun-Sang;Lee, Jung-Hoon
    • Journal of Sensor Science and Technology
    • /
    • v.19 no.6
    • /
    • pp.497-503
    • /
    • 2010
  • This paper reports a chemomechanical explosive sensor based on a thin polymer membrane. The sensor consists of thin parylene membrane and electrodes. Parylene membrane is functionalized with 4-mercaptophenol which interacts strongly with nitrotoluene based explosives. The membrane deflection caused by molecular interaction between the surface and explosives is monitored by capacitance between the membrane and the substrate. To measure the capacitance, electrodes are formed on the membrane and the substrate. While the previous cantilever system requires a bulky optical measuring system, this purely electric monitoring method offers a compact and effective system. Thus, this explosive sensor can be readily miniaturized and used in the field. The developed sensor can reliably detect dinitrotoluene and its limit of detection is evaluated as approximately 110 ppb.

KINETICS OF AUTOTROPHIC DENITRIFICATION FOR THE BIOFILM FORMED ON SULFUR PARTICLES : Evaluation of Molecular Technique on Monitoring Biomass Growth

  • Kim, Sung-Youn;Jang, Am;Kim, I-Tae;Kim, Kwang-Soo;Kim, In-S.
    • Environmental Engineering Research
    • /
    • v.10 no.6
    • /
    • pp.283-293
    • /
    • 2005
  • Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, ${\mu}_{max}$, and the half velocity constant, $K_M$, were estimated as $0.61\;d^{-1}$ and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode.

Effect of Surface Roughness of Counterface on Tribological Characteristics of PTFE and UHMWPE (상대재료의 표면거칠기에 따른 PTFE와 UHMWPE의 마찰 및 마멸 특성)

  • Dong, Sun;Chung, Koo-Hyun;Lee, Kyung-Sick
    • Tribology and Lubricants
    • /
    • v.27 no.6
    • /
    • pp.293-301
    • /
    • 2011
  • Understanding of the tribological characteristics of polytetrafluoroethylen (PTFE) and ultra-high-molecular-weight polyethylene (UHMWPE) is crucial for their applications such as bearing and total joint replacement. In this work, the effect of the surface roughness of carbon steel on the tribological behaviors of PTFE and UHMWPE was experimentally investigated by using block-on-ring tribotester with friction force monitoring capability. It was found that that the amount of material transfer layers of PTFE formed on the carbon steel was significantly larger than those of UHMWPE, which was responsible the lower friction coefficient of PTFE. It was also concluded that the effect of surface roughness of carbon steel on the friction coefficient of UHMWPE was more significant than that of PTFE. For UHMWPE, it was found that the effect of surface roughness of counterface was varied with respect to applied normal force and sliding as well. Based on Archard's wear law, the wear coefficient of PTFE and UHMWPE was calculated to be $3{\times}10^{-5}$ ~ $8{\times}10^{-5}$ and $7{\times}10^{-6}$ ~ $2{\times}10^{-5}$, respectively.

Water table: The dominant control on CH4 and CO2 emission from a closed landfill site

  • Nwachukwu, Arthur N.;Nwachukwu, Nkechinyere V.
    • Advances in environmental research
    • /
    • v.9 no.2
    • /
    • pp.123-133
    • /
    • 2020
  • A time series dataset was conducted to ascertain the effect of water table on the variability in and emission of CH4 and CO2 concentrations at a closed landfill site. An in-situ data of methane/carbon dioxide concentrations and environmental parameters were collected by means of an in-borehole gas monitor, the Gasclam (Ion Science, UK). Linear regression analysis was used to determine the strength of the correlation between ground-gas concentration and water table. The result shows CH4 and CO2 concentrations to be variable with strong negative correlations of approximately 0.5 each with water table over the entire monitoring period. The R2 was slightly improved by considering their concentration over single periods of increasing and decreasing water table, single periods of increasing water table, and single periods of decreasing water table; their correlations increased significantly at 95% confidence level. The result revealed that fluctuations in groundwater level is the key driving force on the emission of and variability in groundgas concentration and neither barometric pressure nor temperature. This finding further validates the earlier finding that atmospheric pressure - the acclaimed major control on the variability/migration of CH4 and CO2 concentrations on contaminated sites, is not always so.

Phytomonitoring of the Genotoxicity of Environmental Pollutants: An Application to Armenian Nuclear Power Plant

  • Kim, Jin Kyu;Aroutiounian, Rouben M.;Nebish, Anna A.;Kim, Jin-Hong
    • Journal of Radiation Industry
    • /
    • v.9 no.4
    • /
    • pp.181-185
    • /
    • 2015
  • Today the biosafety evaluation, a common problem of vital importance, is based on internationally proved test-systems, standards and techniques. The paradigm of biosafety includes multidisciplinary approach, a combination of physical, chemical and biological tests to monitor the environmental level of pollutants and needs to be improved by modern approaches. The genetic risk of environmental pollutions has long been studied by many researchers. In this study, used was the known sensitive plant test-system, clones of plant Tradescantia (spiderwort) able to detect gene mutations (frequency of mutational events and formation of micronuclei) in combination with chemical and, in some instances, with radiological measurements. In addition, male gametophyte generation of fruit trees was applied as bioindicators of genotoxicity. The obtained results did not show any significant increase along with wind direction. As for the male gametophyte assay, the fertility of the investigated fruit-trees near to NPP did not significantly differ from that of the control point. The influence of the NPP on the male generative system of the investigated taxa of fruit trees for the investigated year was not revealed. The system described needs to be expanded by species of interest (human) as there is a difficulty to transfer the revealed dose correlations to humans. The development of this idea includes various levels: population (epidemiological studies), individual, cellular, molecular (DNA), etc.

Analysis of Korean japonica rice cultivars using molecular markers associated with blast resistance genes

  • Suh, Jung-Pil;Roh, Jae-Hwan;Cho, Young-Chan;Han, Seong-Sook;Jeon, Yong-Hee;Kang, Kyung-Ho;Kim, Yeon-Gyu
    • Korean Journal of Breeding Science
    • /
    • v.40 no.3
    • /
    • pp.215-222
    • /
    • 2008
  • Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

Unrecorded Fungal Species Isolated from Indoor Air in the Log Bed- and Sawdust Media-based Mushroom Cultivation Houses (원목 및 톱밥배지 버섯 재배사 내 실내 공기서 분리한 미기록 진균 보고)

  • Ahn, Geum Ran;Kim, Ji Eun;Kim, Jun Young;Kim, Seong Hwan
    • The Korean Journal of Mycology
    • /
    • v.46 no.4
    • /
    • pp.495-503
    • /
    • 2018
  • Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and ${\beta}-tubulin$ gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

Current Methods of Circulating Tumor Cell Detection (순환종양세포 검출 기술)

  • Lim, Minji;Cho, Yoon-Kyoung
    • The Korean journal of helicobacter and upper gastrointestinal research
    • /
    • v.18 no.3
    • /
    • pp.157-161
    • /
    • 2018
  • Liquid biopsy, the analysis of circulating biomarkers from peripheral blood, such as circulating tumor cells (CTCs) and circulating tumor DNA, and exosomes, offers a less invasive, new source of cancer-derived materials that may reflect the status of the disease better and thereby contribute to personalized treatment. Recent advances in microfluidics and molecular analysis technologies have resulted in greatly improved CTC enumeration and detection. In this article, we review commercially available technologies used to isolate CTCs from peripheral blood, including immunoaffinity and label-free, physical property-based isolation methods. Although enormous technological progress has been made, especially within the last decade, only a few CTC detection methods have been approved for routine clinical use. Here, we provide an overview of the current CTC isolation methods and examples of their potential application for early diagnosis, prognosis, treatment monitoring, and prediction of resistance to cancer therapy. Furthermore, the challenges that remain to be addressed before such tools are implemented for routine use in clinical settings are discussed.

Seasonal Changes in Cyanobacterial Diversity of a Temperate Freshwater Paldang Reservoir (Korea) Explored by using Pyrosequencing

  • Boopathi, Thangavelu;Wang, Hui;Lee, Man-Duck;Ki, Jang-Seu
    • Korean Journal of Environmental Biology
    • /
    • v.36 no.3
    • /
    • pp.424-437
    • /
    • 2018
  • The incidence of freshwater algal bloom has been increasing globally in recent years and poses a major threat to environmental health. Cyanobacteria are the major component of the bloom forming community that must be monitored frequently. Their morphological identities, however, have remained elusive, due to their small size in cells and morphological resemblances among species. We have analyzed molecular diversity and seasonal changes of cyanobacteria in Paldang Reservoir, Korea, using morphological and 16S rRNA pyrosequencing methods. Samples were collected at monthly intervals from the reservoir March-December 2012. In total, 40 phylotypes of cyanobacteria were identified after comparing 49,131 pyrosequence reads. Cyanobacterial genera such as Anabaena, Aphanizomenon, Microcystis and Synechocystis were predominantly present in samples. However, the majority of cyanobacterial sequences (65.9%) identified in this study were of uncultured origins, not detected morphologically. Relative abundance of cyanobacterial sequences was observed as high in August, with no occurrence in March and December. These results suggested that pyrosequencing approach may reveal cyanobacterial diversity undetected morphologically, and may be used as reference for studying and monitoring cyanobacterial communities in aquatic environments.

Chronic non-bacterial osteomyelitis in the jaw

  • Kim, Soung Min;Lee, Suk Keun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • v.45 no.2
    • /
    • pp.68-75
    • /
    • 2019
  • Chronic recurrent multifocal osteomyelitis (CRMO) is one of the most severe form of chronic non-bacterial osteomyelitis (CNO), which could result in bone and related tissue damage. This autoinflammatory bone disorder (ABD) is very difficult for its clinical diagnosis because of no diagnostic criteria or biomarkers. CRMO in the jaw must be suspected in the differential diagnosis of chronic and recurrent bone pain in the jaw, and a bone biopsy should be considered in chronic and relapsing bone pain with swelling that is unresponsive to treatment. The early diagnosis of CRMO in the jaw will prevent unnecessary and prolonged antibiotic usage or unnecessary surgical intervention. The updated researches for the identification of genetic and molecular alterations in CNO/CRMO should be studied more for its correct pathophysiological causes and proper treatment guidelines. Although our trial consisted of reporting items from Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), there are very few articles of randomized controlled trials. This article was summarized based on the author's diverse clinical experiences. This paper reviews the clinical presentation of CNO/CRMO with its own pathogenesis, epidemiology, recent research studies, and general medications. Treatment and monitoring of the jaw are essential for the clear diagnosis and management of CNO/CRMO patients in the field of dentistry and maxillofacial surgery.