• Title/Summary/Keyword: molecular distribution

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Effect of Polymerization Conditions on the Characteristics of Polyvinyl Acetate Emulsions

  • Youn, Hye-Jung;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.30 no.4
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    • pp.28-34
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    • 1998
  • Polyvinyl acetate emulsion has been widely used as adhesives for wood and paper, paint additives and binders for fiber, leather, and other materials because it is an excellent adhesive with many advantages including low in toxicity risks and manufacturing cost. It is expected the consumption of polyvinyl acetate emulsion as adhesives will increase in cigarette industry as well as in paperboard coating industry. Recently the operation speed of the cigarette tip wrapper increased so substantially that improvement of the emulsion properties is required including good wet tack development, narrow and controlled particle size distribution, low viscosity, etc. In this study the effects of such polymerization conditions as the type and amount of emulsifier, internal or external plasticizing, and emulsification methods on the viscosity and particle size of polyvinyl acetate emulsions were examined. Results showed that polyvinyl alcohol with a high degree of hydrolysis and low molecular weight and nonionic surfactants are superior to anionic surfactant in improving adhesion and emulsion stability. They also tend to produce emulsions with smaller particle size. External plasticization with dipropylene glycol dibenzoate was more effective in improving flexibility than internal plasticization with butyl acrylate. Monomer emulsification under high shear was more effective in decreasing the particle size.

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Studies on the Adsorbents for Cigarette Filter I. Effect of Pore Voume Distribution and Specific Area of Adsorbents on the Removal Efficiency of Smoke Components by Triple Filter (담배필터용 흡착제에 관한 연구 제1보. 흡착제의 종류와 동공특성이 담배연기성분 제거능에 미치는 영향)

  • 박태무;이영택;김성한;오영일
    • Journal of the Korean Society of Tobacco Science
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    • v.10 no.1
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    • pp.75-82
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    • 1988
  • Cigarettes were made using a triple filter with several porous materials in its cavity. The removal effect of the adsorbents on carbon monoxide and hydrogen cyanide in cigarette smoke was investigated with the variation of their surface area and pore volume distributions. Several attempts were made to activated coconut shell based char under the fixed steam purging rate. 1. The specific surface area increased in number of micropore. It was found for transitional pore to have a little effect on the total surface area. 2. A Small amount of the particulate matter adsorbed on the adsorbents with transitional pores, Zeolite showed a little effect on the carbon monoxide adsorption though its small pore volume, but there was no significant difference in the adsorption capacity zeolite and the others. 3. In the adsorption for hydrogen cyanide as a vapor phase in cigarette smoke, the adsorption effect of the adsorbents increased remarkably with increasing their surface area and number of micropore. It was considered that the adsorbents with small pore volume like molecular seive 4A, in which the capillary diffusion of adsorbates could not be able, would not be effective for the adsorption of hydrogen cyanide.

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Anti-inflammatory effects of a methanol extract from Pulsatilla koreana in lipopolysaccharide-exposed rats

  • Lee, Sang-Hyun;Lee, Eun;Ko, Young-Tag
    • BMB Reports
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    • v.45 no.6
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    • pp.371-376
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    • 2012
  • To investigate the therapeutic effect of a Korean herbal medicine Pulsatilla koreana as an anti-septic agent, anti-inflammatory effects of the herbal medicine were determined in lipopolysaccharide (LPS)-exposed rats. Treatment with a methanol extract from Pulsatilla koreana significantly inhibited LPS-induced inflammatory responses. Results from ELISA analysis showed that Pulsatilla koreana decreased the plasma and hepatic levels of pro-inflammatory cytokines such as IL-$1{\beta}$, IL-6, TNF-${\alpha}$ while increased the level of anti-inflammatory cytokine IL-10 in LPS-exposed rats. Pulsatilla koreana also decreased the plasma levels of other inflammatory mediators such as $NO_3{^-}/NO_2{^-}$, ICAM-1, $PGE_2$, and CINC-1 in LPS-exposed rats. Although no significant effects were observed in the phagocytic activities, the distribution of lymphocyte population was significantly shifted by the treatment with Pulsatilla koreana. All together, Pulsatilla koreana exerts anti-inflammatory activities in the immune-challenged animals implicating that this Korean herbal medicine is therapeutically useful for the treatment of inflammatory diseases like sepsis.

Codon Usage Patterns of Tyrosinase Genes in Clonorchis sinensis

  • Bae, Young-An
    • Parasites, Hosts and Diseases
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    • v.55 no.2
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    • pp.175-183
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    • 2017
  • Codon usage bias (CUB) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. In this study, genetic indices associated with CUB, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the Clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. The relative synonymous codon usage patterns substantially differed among tyrosinase genes examined. In a neutrality analysis, the correlation between $GC_{12}$ and $GC_3$ was statistically significant, and the regression line had a relatively gradual slope (0.218). NC-plot, i.e., $GC_3$ vs effective number of codons (ENC), showed that most of the tyrosinase genes were below the expected curve. The codon adaptation index (CAI) values of the platyhelminth tyrosinases had a narrow distribution between 0.685/0.714 and 0.797/0.837, and were negatively correlated with their ENC. Taken together, these results suggested that CUB in the tyrosinase genes seemed to be basically governed by selection pressures rather than mutational bias, although the latter factor provided an additional force in shaping CUB of the C. sinensis and Opisthorchis viverrini genes. It was also apparent that the equilibrium point between selection pressure and mutational bias is much more inclined to selection pressure in highly expressed C. sinensis genes, than in poorly expressed genes.

Development of Doubled-haploid Population and Construction of Genetic Map Using SSR Markers in Rice (벼의 Doubled-haploid 집단육성과 SSR 마커를 이용한 유전자 지도작성)

  • Kim, Kyung-Min;Nam, Wu-Il;Kwon, Yong-Sham;Sohn, Jae-Keun
    • Journal of Plant Biotechnology
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    • v.31 no.3
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    • pp.179-184
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    • 2004
  • A doubled-haploid (DH) population was developed through anther culture of F$_1$ plants obtained from a cross between a japonica cultivar, 'Nagdongbyeo', as male parent and a indica cultivar, 'Samgangbyeo', as female parent. Segregation modes for plant length, culm length, panicle length, third internode length, and days to heading in the DH lines showed nearly normal distribution with wide range of variation. A molecular map with 136 simple sequence repeat (SSR) markers was constructed using the DH population. The total map distance was 1,909 cM and the average interval of marker distance was 14 cM.

Expert Design Evaluation System for injection Molding

  • Kim, Sang-Gook;Huh, Yong-Jeong
    • International Journal of Precision Engineering and Manufacturing
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    • v.2 no.1
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    • pp.62-75
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    • 2001
  • The design and manufacture of injection molded polymeric parts with desired properties is a costly process dominated by empiricism including repeated modification of actual tooling. This paper presents and expert design evaluation system which can predict the mechanical performance of a molded product and diagnose the design before the actual mold is machined. The knowledge-based system synergistically combines a rule-based expert system with CAE programs. An iterative boundary pressure reflection method(IBPR) is developed to automate the cavity filling simulation program and to predict thermo-mechanical properties of a molded part precisely. Mathematical models of weldline and frozen-in molecular orientation are established to determine the spatial variation of microstructural anisotropies of a molded part from the result of cavity filling simulation. The strength ellipse is devised as and index which represents th spatial distribution of the microstructural anisotropies of a molded part, Heuristic knowledge of injection molding, flow simulation, and mechanical performance prediction is formalized as rules of an expert consultation system. The expert system interprets the analytical results of the process simulation, predicts the performance, evaluates the design and generates recommendations for optimal design alternative.

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Recovery of Silk Sericin from Soap-Alkaline Degumming Solution

  • Yang, Yesol;Lee, Sang Mi;Lee, Han Sol;Lee, Ki Hoon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.27 no.1
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    • pp.203-208
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    • 2013
  • Sericin is usually abandoned after the degumming process. However, it could be a valuable bioresource if an economically efficient recovery process could be set up. In this study, sericin was recovered directly from the degummed waste solution by adding calcium chloride, which induced the precipitation of the surfactant, sodium oleate, by charge interaction. The recovery yield was maximum when 10% of calcium chloride was added. Further increase in the calcium chloride concentration induced the precipitation of sericin. The recovered sericin had a molecular weight distribution similar to that of the hot-water-extracted sericin; but some highmolecular- weight sericin could not be recovered. The secondary structure and amino acid composition of the recovered sericin were similar to those of conventional hot-water-extracted sericin. We expect that sericin recovered from the degummed waste solution could be an alternative to the hot-water-extracted sericin, which is widely used in various applications.

Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular Discrimination of Satsuma Dwarf Virus Collected from Citrus Orchards

  • Kato, Mitsuhiro;Tomimura, Kenta;Ishii, Kanako
    • The Plant Pathology Journal
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    • v.36 no.5
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    • pp.509-514
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    • 2020
  • Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA. The samples scored as positive by either DAS-ELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

Molecular Detection of Human Enteric Viruses in Urban Rivers in Korea

  • Lee, Cheong-Hoon;Kim, Sang-Jong
    • Journal of Microbiology and Biotechnology
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    • v.18 no.6
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    • pp.1156-1163
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    • 2008
  • We performed RT-nested PCR to study the distribution of human enteric viruses in urban rivers in Korea. During 2002-2003, water samples were collected from four rivers in Gyeonggi Province, South Korea. Among 58 samples, 45 (77.6%), 32 (55.2%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) showed positive results with adenoviruses (AdVs), enteroviruses (EVs), reoviruses (ReVs), hepatitis A viruses (HAVs), rotaviruses (RoVs), and sapoviruses (SVs), respectively. According to the binary logistic regression model, the occurrence of each enteric virus, except ReVs and HAVs, was not statistically correlated with the water temperature and levels of fecal coliforms (P<0.05). AdVs were most often detected; only 4 samples (6.9%) were negative for AdVs while positive for other enteric viruses in the studied sites. Our results indicated that monitoring human enteric viruses is necessary to improve microbial quality, and that AdVs detection by PCR can be a useful index for the presence of other enteric viruses in aquatic environments.

New Fluid Flow System for Simulation of Mechanical Loading to Bone Cells During Human Gait Cycle

  • Ahn, Jae-Mok
    • Journal of Biomedical Engineering Research
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    • v.28 no.3
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    • pp.377-386
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    • 2007
  • Mechanical loading to bone cells using simple sine wave or constant wave fluid flow has been widely used for in vitro experiments. Human gait is characterized by a complex loading to bones of lower extremities which results from a series of events consisting of heel strike, foot flat and push-off during the stance phase of the gait cycle. Telemetric force analyses have shown that human femora are subject to multiphasic loading. Therefore, it would be ideal if the physiologic loading conditions during human walking can be used for in vitro mechanotransduction studies. Here, for a mechanotransduction study, we develop it fluid flow system (FFS) in order to simulate human physiologic mechanicalloading on bone cells. The development methods of the FFS including the COR (Center for Orthopedic Research), monitor program are presented. The FFS could generate various multiphasic loading conditions of human gaits with output flow. Wall shear distribution was very uniform, with 81 % of the effective loading area of the culture on a glass slide. Our results demonstrated that the FFS, provide a new translational approach for unveiling molecular mechanotransduction pathways in bone cells.