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Molecular Detection of Human Enteric Viruses in Urban Rivers in Korea  

Lee, Cheong-Hoon (School of Biological Sciences, College of Natural Sciences, Seoul National University)
Kim, Sang-Jong (School of Biological Sciences, College of Natural Sciences, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.6, 2008 , pp. 1156-1163 More about this Journal
Abstract
We performed RT-nested PCR to study the distribution of human enteric viruses in urban rivers in Korea. During 2002-2003, water samples were collected from four rivers in Gyeonggi Province, South Korea. Among 58 samples, 45 (77.6%), 32 (55.2%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) showed positive results with adenoviruses (AdVs), enteroviruses (EVs), reoviruses (ReVs), hepatitis A viruses (HAVs), rotaviruses (RoVs), and sapoviruses (SVs), respectively. According to the binary logistic regression model, the occurrence of each enteric virus, except ReVs and HAVs, was not statistically correlated with the water temperature and levels of fecal coliforms (P<0.05). AdVs were most often detected; only 4 samples (6.9%) were negative for AdVs while positive for other enteric viruses in the studied sites. Our results indicated that monitoring human enteric viruses is necessary to improve microbial quality, and that AdVs detection by PCR can be a useful index for the presence of other enteric viruses in aquatic environments.
Keywords
Enteric virus; RT-nested PCR; adenovirus; fecal coliforms; urban river;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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1 American Public Health Association. 1995. Standard Methods for the Examination of Water and Wastewater, 19th Ed. American Public Health Association, Washington, D.C
2 Choi, J.-W., Y.-K. Kim, H.-J. Kim, W. Lee, and G. H. Seong. 2006. Lab-on-a-chip for monitoring the quality of raw milk. J. Microbiol. Biotechnol. 16: 1229-1235   과학기술학회마을
3 Lee, D.-G., S. J. Park, and S.-J. Kim. 2007. Influence of pipe materials and VBNC cells on culturable bacteria in a chlorinated drinking water model system. J. Microbiol. Biotechnol. 17: 1558-1562   과학기술학회마을
4 Leparc, I., M. Aymard, and F. Fuchs. 1994. Acute, chronic and persistent enterovirus and poliovirus infection: Detection of viral genome by semi-nested PCR amplification in culture-negative samples. Mol. Cell. Probes 8: 487-495   DOI   ScienceOn
5 Morace, G., F. A. Aulicino, C. Angelozzi, L. Costanzo, F. Donadio, and M. Rapicetta. 2002. Microbial quality of wastewater: Detection of hepatitis A virus by reverse transcriptase-polymerase chain reaction. J. Appl. Microbiol. 92: 828-836   DOI   ScienceOn
6 Pina, S., M. Puig, F. Lucena, J. Jofre, and R. Girones. 1998. Viral pollution in the environment and in shellfish: Human adenovirus detection by PCR as an index of human viruses. Appl. Environ. Microbiol. 40: 805-809
7 Sellwood, J., J. Dadswell, and J. Slade. 1981. Viruses in sewage as an indicator of their presence in the community. J. Hyg. 86: 217-225   DOI   ScienceOn
8 Tani, N., Y. Dohi, N. Kurumatani, and K. Yonemasu. 1995. Seasonal distribution of adenoviruses, enteroviruses and reoviruses in urban river water. Microbiol. Immunol. 39: 577-580   DOI
9 Taylor, M. B., N. Cox, M. A. Vrey, and W. O. Grabow. 2001. The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa. Water Res. 35: 2653-2660   DOI   ScienceOn
10 Ward, R. L. and E. W. Akin. 1984. Minimum infective dose of animal viruses. CRC Crit. Rev. Environ. Contam. 14: 297-310   DOI
11 Gerba, C. P. and J. B. Rose. 1990. Viruses in source and drinking water. In G. A. McFeters (ed.), Drinking Water Microbiology. Springer-Verlag, New York
12 Fong, T. T. and E. K. Lipp. 2005. Enteric viruses of humans and animals in aquatic environments: Health risks, detection, and potential water quality assessment tools. Microbiol. Mol. Biol. Rev. 69: 357-371   DOI   ScienceOn
13 Leary, T. P., J. C. Erker, M. L. Chalmers, A. T. Cruz, J. D. Wetzel, S. M. Desai, I. K. Mushahwar, and T. S. Dermody. 2002. Detection of mammalian reovirus RNA by using reverse transcription-PCR: Sequence diversity within the $\lambda$3-encoding L1 gene. J. Clin. Microbiol. 40: 1368-1375   DOI   ScienceOn
14 Lee, D.-G., S.-J. Kim, and S. J. Park. 2006. Effect of reservoirs on microbiological water qualities in a drinking water distribution system. J. Microbiol. Biotechnol. 16: 1060-1067   과학기술학회마을
15 Muniain-Mujika, I., M. Calvo, F. Lucena, and R. Girones. 2003. Comparative analysis of viral pathogens and potential indicators in shellfish. Int. J. Food Microbiol. 38: 75-85
16 Meng. X.-J., R. H. Purcell, P. G. Halbur, J. R. Lehman, D. H. Webb, T. S. Tsareva, J. S. Haynes, B. J. Thacker, and S. U. Emerson. 1997. A novel virus in swine is closely related to the human hepatitis E virus. Proc. Natl. Acad. Sci. USA 94: 9860- 9865
17 Wyn-Jones, A. P. and J. Sellwood. 2001. A review: Enteric viruses in the aquatic environment. J. Appl. Microbiol. 91: 945-962   DOI   ScienceOn
18 Lee, S.-H., C. Lee, K. W. Lee, H. B. Cho, and S.-J. Kim. 2005. The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure. J. Appl. Microbiol. 98: 1020-1029   DOI   ScienceOn
19 Lee, S.-H. and S.-J. Kim. Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea. Water Res. 36: 248-256   DOI   ScienceOn
20 Lee, C., S.-H. Lee, E. Han, and S.-J. Kim. 2004. Use of cell culture-PCR assay based on combination of A549 and BGMK cell lines and molecular identification as a tool to monitor infectious adenoviruses and enteroviruses in river water. Appl. Environ. Microbiol. 70: 6695-6705   DOI   ScienceOn
21 Formiga-Cruz, M., A. Hundesa, P. Clemente-Casares, N. Albinana- Gimenez, A. Allard, and R. Girones. 2005. Nested multiplex PCR assay for detection of human enteric viruses in shellfish and sewage. J. Virol. Methods 125: 111-118   DOI   ScienceOn
22 Chapron, C. D., N. A. Ballester, J. H. Fontaine, C. N. Frades, and A. B. Margolin. 2000. Detection of astroviruses, enteroviruses, and adenovirus type 40 and 41 in surface waters collected and evaluated by the Information Collection Rule and an integrated cell culture-nested PCR procedure. Appl. Environ. Microbiol. 66: 2520-2525   DOI   ScienceOn
23 Cho, H.-B., S.-H. Lee, J.-C. Cho, and S.-J. Kim. 2000. Detection of adenoviruses and enteroviruses in tap water and river water by reverse transcription multiplex PCR. Can. J. Microbiol. 46: 417-424   DOI   ScienceOn
24 Duizer, E., K. J. Schwab, F. H. Neill, R. L. Atmar, M. P. G. Koopmans, and M. K. Estes. 2004. Laboratory efforts to cultivate noroviruses. J. Gen. Virol. 85: 79-87   DOI   ScienceOn
25 Allard, A., B. Albinsson, and G. Wadell. 1992. Detection of adenoviruses in stools from healthy persons and patients with diarrhea by two-step polymerase chain reaction. J. Med. Virol. 37: 149-157   DOI   ScienceOn
26 Formiga-Cruz, M., A. K. Allard, A.-C. Conden-Hanson, K. Henshilwood, B. E. Hernroth, J. Cofre, et al. 2003. Evaluation of potential indicators of viral contamination in shellfish and their applicability to diverse geographical areas. Appl. Environ. Microbiol. 69: 1556-1563   DOI   ScienceOn
27 Choi, S. and S. C. Jiang. 2005. Real-time PCR quantification of human adenoviruses in urban rivers indicates genome prevalence but low infectivity. Appl. Environ. Microbiol. 71: 7426-7433   DOI   ScienceOn
28 Okada, M., K. Shinozaki, T. Ogawa, and I. Kaiho. 2002. Molecular epidemiology and phylogenetic analysis of Sapporo-like viruses. Arch. Virol. 147: 1445-1451   DOI   ScienceOn
29 Lewis, G. D. and T. G. Metcalf. 1988. Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples. Appl. Environ. Microbiol. 54: 1983-1988
30 Baggi, F. and R. Peduzzi. 2000. Genotyping of rotaviruses in environmental water and stool samples in southern Switzerland by nucleotide sequence analysis of 189 base pairs at the 5' end of the VP7 gene. J. Clin. Microbiol. 38: 3681- 3685
31 Formiga-Cruz, M., G. Tofiño-Quesada, S. Bofill-Mas, D. N. Lees, K. Henshilwood, A. K. Allard, et al. 2002. Distribution of human virus contamination in shellfish from different growing areas in Greece, Spain, Sweden, and the United Kingdom. Appl. Environ. Microbiol. 68: 5990-5998   DOI   ScienceOn