• 대한기계학회논문집B
• /
• 제33권2호
• /
• pp.101-106
• /
• 2009

The disjoining pressure is an important physical property in modeling the small-scale transport phenomena on thin film. It is a very useful definition in characterizing the non-continuum effects that are not negligible in heat and mass transport of the film thinner than submicro-scales. We present the calculated values of disjoining pressure of He, Kr and Xe thin films absorbed on graphite substrate using Molecular Dynamics Simulation (MD). The disjoining pressure is accurately calculated in the resolution of a molecular scale of the film thickness. The characteristics of the pressure are discussed regarding the molecular nature of the fluid system such as molecular diameter and intermolecular interaction parameters. The MD results are also compared with those based on the continuum approximation of the slab-like density profile and the results on other novel gases in the previous study. The discrepancies of the continuum model with MD results are shown in all three configurations and discussed in the view point of molecular features.

• Journal of Microbiology and Biotechnology
• /
• 제26권1호
• /
• pp.171-179
• /
• 2016

In this study, we compared the virulence-associated factors of Acinetobacter baumannii complex species. Sixty-three isolates of five A. baumannii complex species, including 19 A. baumannii, 15 A. nosocomialis, 13 A. seifertii, 13 A. pittii, and 3 A. calcoaceticus isolates, were included in this study. For all isolates, biofilm formation, A549 cell adherence, resistance to normal human serum, and motility were evaluated. A. baumannii complex isolates showed diversity in biofilm formation, A549 cell adherence, and serum resistance, and no strong positive relationships among these virulence characteristics. However, A. seifertii showed relatively consistent virulence-associated phenotypes. In addition, A. baumannii clone ST110 exhibited consistently high virulence-associated phenotypes. Motility was observed in seven isolates, and all four A. baumannii ST110 isolates showed twitching motility. Although some inconsistencies in virulence-associated phenotypes were seen, high virulence characteristics were observed in A. seifertii, which has been mainly reported in Korea and shows high rates of colistin resistance.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제20권7호
• /
• pp.70-79
• /
• 2015

A sample of soil humic acid (HA) was divided by ultrafiltration (UF) into five fractions of different molecular size (UF₁: > 300, UF₂: 100~300, UF₃: 30~100, UF₄: 10~30, UF₅: 1~10 kilodaltons). Apparent average molecular weight (M_w) of the HA fractions were measured using high performance size exclusion chromatography (HPSEC), and the chemical and structural properties of the five HA fractions were characterized by elemental compositions (H/C, O/C and w ((2O + 3NH)/ C)) and ultraviolet-visible absorption ratios (SUVA, A_4/6). The organic carbon normalized-sorption coefficients (Koc) for the binding of phenanthrene to the HA fractions were determined by fluorescence quenching and relationship between the sorption coefficients and structural characteristics of the HA fractions were investigated. The elemental analysis and UV-vis spectral data indicated that the HA fractions with higher molecular weights have grater aliphatic character and lower contents of oxygen, while the HA fractions with lower molecular size have greater aromatic character and molecular polarity that correspond to greater SUVA and internal oxidation values (w). The log Koc values (L/kg C) were gradual increased from 4.45 for UF₅ to 4.87 for UF1. The correlation study between the structural descriptors of the HA fractions and log Koc values of phenanthrene show that the magnitude of Koc values positively correlated with $M_w$ and H/C, while negatively correlated with the independent descriptors of the O/C, w, SUVA and A_4/6.

• 한국잠사곤충학회지
• /
• 제45권1호
• /
• pp.46-57
• /
• 2003

정련 액에서 수용성 세리신을 얻고, 남은 난용성 세리신을 여러가지 효소로 가수분해하여, 효소 가수분해 특성을 알아보고. 아미노산 조성과 분자량을 조사하여 다음과 같은 결론을 얻었다. 1. 효소 가수분해 시 kojizyme과 flavourzyme의 최적조건은 pH 7, 온도 5$0^{\circ}C$이었고, protamex와 alcalase는 처리 pH의 증가에 따라 활성이 높았고 최적온도는 6$0^{\circ}C$이었다. 2 효소 가수분해 시 용해도 측정에서는 효소 처리농도 증가에 따라 용해도도 높았으며, 4시간 이상의 처리에서는 아주 소폭으로 용해도가 증가하였다. 3. 효소 가수분해 시 흡광도를 이용한 용해도 측정에서는 효소 처리농도 증가에 따라 흡광도도 높았으며. 4시간 이상의 처리에서도 계속하여 흡광도가 증가하였다. 4. 효소 가수분해 시 말단기 정량에 의한 평균중합도 측정에서는 처리시간과 농도의 증가에 따라 중합도가 감소하였다. 5. 아미노산 분석 결과 정련 후 채취한 low와 high의 아미노산 조성이 비슷하고, PK와 PP및 PA의 아미노산 조성이 비슷하였으며, low와 high는 PK, PP, PA보다 세린과 타이로신의 함량이 많았고, 프롤린은 검출되지 않았다. 6. GPC에 의한 분자량 측정결과 중량평균분자량(Mw) 크기는 PK＞high＞PP＞low＞PA 순서이었으며, PK와 PA의 Mw는 각각 9,800, 905이었다

• BMB Reports
• /
• 제40권5호
• /
• pp.783-790
• /
• 2007

Receptor binding plays an important role in determining host specificity of the Bacillus thuringiensis Cry $\delta$-endotoxins. Mutations in domains II and III have suggested the participation of certain residues in receptor recognition and insect specificity. In the present study, we expressed the cloned domain II-III fragment of Cry4Ba and examined its binding characteristics to mosquito-larval midgut proteins. The 43-kDa Cry4Ba-domain II-III protein over-expressed in Escherichia coli as inclusion bodies was only soluble when carbonate buffer, pH 10.0 was supplemented with 4M urea. After renaturation via stepwise dialysis and subsequent purification, the refolded domain II-III protein, which specifically reacts with anti Cry4Ba-domain III monoclonal antibody, predominantly exists as a $\beta$-sheet structure determined by circular dichroism spectroscopy. In vitro binding analysis to both histological midgut tissue sections and brush border membrane proteins prepared from susceptible Aedes aegypti mosquito-larvae revealed that the isolated Cry4Ba-domain II-III protein showed binding functionality comparable to the 65-kDa full-length active toxin. Altogether, the data present the 43-kDa Cry4Ba fragment comprising domains II and III that was produced in isolation was able to retain its receptor-binding characteristics to the target larval midgut proteins.

• 한국물환경학회지
• /
• 제23권6호
• /
• pp.843-849
• /
• 2007

Monitoring of NOM characteristics is important for improving removal efficiency of natural organic matter (NOM) in water treatment processes. In this study, several NOM characteristics, which include specific UV absorbance (SUVA), total carbonate content, molecular weight distribution, and fluorescence properties, were measured using samples collected from a pilot-scale water treatment plant consisting of coagulation/flocculation (C/F), filtration, ozonation and granular activated carbon (GAC) processes. The highest removal of NOM was observed in C/F and filtration processes as demonstrated by the reduction of dissolved organic carbon (DOC) by 25% and 21%, respectively. Despite nearly no change in DOC, however, the lowest SUVA value and the highest total carbohydrate content were observed in the sample from ozonation process. This indicates that non-degradable aromatic compounds become depleted and biodegradable organic compounds are enriched during the process. Comparison of synchronous fluorescence spectra of the samples showed that ozoation process increased protein-like fluorescence while it decreased fulvic-like and terrestrial humic-like fluorescence. Consistently, a slight peak of protein-like fluorescence was observed in the sample from ozonation process. The greatest change in molecular weight distributions of the samples was observed in C/F process. Comparison of size exclusion chromatogram of the samples revealed that NOM fractions with the molecular weight greater than 2000 Da were reduced by over 90% after C/F process. SUVA values and total carbohydrate content of the samples were well correlated with a ratio of protein-like fluorescence and terrestrial humic-like fluorescence intensities with the correlation coefficients of 0.99 and 0.91, respectively. This suggests that synchronous fluorescence properties of NOM could be used as useful tolls for monitoring changes of some NOM characteristics during water treatment processes.

• ALGAE
• /
• 제29권2호
• /
• pp.57-73
• /
• 2014

Amphora Ehrenberg ex Kutzing sensu lato is a common and widespread benthic diatom genus with a taxonomy that has been under continual revision, particularly based on molecular analyses. Although Amphora species have been studied using modern microscopy in recent years, there has not been much progress on molecular characterization of the species, especially in Asia. In this study of Amphora, sampling was carried out from September 2009 to August 2010 in Korean coastal waters. The morphological and molecular characteristics of eight Amphora sensu lato were examined: Amphora marina, A. proteus, Halamphora costata, H. coffeaeformis, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. Based on previous accounts, morphology suggested that A. marina and A. proteus belong to the subgenus Amphora Cleve, which have smooth girdle bands and rather coarse and very distinct areolae on the valve. The other species, H. coffeaeformis, H. costata, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. belong to the subgenus Halamphora Cleve, which was recently elevated to generic status by Levkov 2009, have plicate girdle bands, puncta which do not form straight longitudinal lines, valves which have a narrow ventral portion and apices that are generally rostrate-capitate and recurved. In agreement with analysis based on morphological characteristics, phylogenetic analysis based on small subunit rDNA suggested that the eight Amphora sensu lato species were not a monophyletic group as the morphological classification. Also, the results of molecular work and statistical analysis on all these Amphora sensu lato combined with phylogenic analysis on our geographically representative samples give strong evidence that Halamphora Levkov is independent of Amphora Cleve. Furthermore, in this study, Amphora terroris was transferred Halamphora as Halamphora terroris (Ehrenberg) Wang comb. nov. and Amphora marina was recorded for the first time in Korea.

• 멤브레인
• /
• 제30권6호
• /
• pp.433-442
• /
• 2020

본 연구에서는 수전해용 ionomer의 분자동역학 전산모사 모델 제작을 위하여, 과량의 물 분자가 존재하는 수전해 시스템의 특성을 반영한 ionomer 모델을 제작한 후, 기존 연료전지용 전해질막 전산모사 조건에 맞춰 제작한 ionomer 모델과 비교하였다. 최종적으로 얻어진 모델은 과불소계 ionomer의 중요 특징 중 하나인 명확한 상분리 및 수화채널이 관찰되었으며, 과량의 물 및 높은 운전 온도 조건에서도 물에 녹지 않고 안정된 구조를 나타내었다. 제조된 ionomer 모델에서는 과량의 물분자로 인한 이온 희석 효과로 이온 전달 성능 감소가 나타났으며, 반대로 수소 기체의 투과는 더 증가할 것으로 분석되었다. 따라서 이러한 수전해 시스템의 특성을 반영한 수전해용 ionomer 분자 구조 설계 전략이 필요하고, 분자동역학 전산모사 연구 시에도 이를 감안한 수전해용 ionomer 모델 제작이 필요하다.

• 한국정보디스플레이학회:학술대회논문집
• /
• 한국정보디스플레이학회 2004년도 Asia Display / IMID 04
• /
• pp.1200-1203
• /
• 2004

We investigated the molecular aligning capability of a polymer layer containing ceramic nanoparticles which can be used as a gate insulator of organic thin-film transistors (OTFTs). Because of the enhanced dielectric properties arising from the nanoparticles and molecular aligning properties of the polymer, the composite layer provides excellent mobility characteristics of the OTFTs.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제7권1호
• /
• pp.5-10
• /
• 2003

The nuclear polyhedrosis virus (NPV) resistance of silkworm is controlled by a pair of dominant genes on autosome and micro-effect modificator genes on sex chromosome Z and has the phenomenon of patroclinal inheritance. Based on its hereditary characteristics, methods of preparing near isogenic lines and their $F_2$ populations for screening molecular markers were designed.