• Title/Summary/Keyword: molecular analyses

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Molecular Recognition : ${\alpha}$-Cyclodextrin and Aspirin Inclusion Complexation

  • Hee Sook Choi
    • Bulletin of the Korean Chemical Society
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    • v.13 no.5
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    • pp.474-479
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    • 1992
  • Molecular interaction between ${\alpha}$-cyclodextrin and aspirin was studied by UV, $^2H$-NMR and $^2H$-NMR spectroscopy analyses for solution complex and by FT-IR analyses for solid complex. The inclusion structure provides a basic understanding of the aspirin and ${\alpha}$-cyclodextrin interaction.

Molecular analyses and reproductive structure to verify the generic relationships of Hypnea and Calliblepharis (Cystocloniaceae, Gigartinales), with proposal of C. saidana comb. nov.

  • Yang, Mi Yeon;Kim, Myung Sook
    • ALGAE
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    • v.32 no.2
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    • pp.87-100
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    • 2017
  • The genera Hypnea and Calliblepharis of the family Cystocloniaceae are discriminated by their female reproductive structure, especially in the formation of carposporangia and gonimoblasts. Hypnea saidana, once classified based on obsolete evidence, has not been studied phylogenetically using molecular analysis and detailed reproductive structure though it shares many morphologic features with the genus Calliblepharis. To provide better understanding of generic relationship of H. saidana with Hypnea and Calliblepharis, we carried out molecular analyses using the nuclear-encoded small subunit ribosomal DNA (SSU) and chloroplast-encoded large subunit of the RuBisCO (rbcL), and exact morphological observations focusing on the reproductive structures of wild specimens. Our molecular phylogeny showed that H. saidana is closely related to Calliblepharis, but distinct from the clade of Hypnea. Female reproductive structure of H. saidana characterized by upwardly developing chains of carposporangia, central reticulum of cell, and gonimoblast filaments not connected to the pericarp provides definite evidence to assign the taxonomic position of this species to Calliblepharis. Based on our combined molecular and morphological analyses, we have proposed Calliblepharis saidana comb. nov., expanding the distribution of Calliblepharis habitat from the eastern Atlantic South Africa, the northern Indian Ocean, Australasia, and Brazil to the western Pacific Ocean.

A new record of brown algae, Papenfussiella densa from Dok-do, Korea

  • Won, Boo Yeon
    • Korean Journal of Environmental Biology
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    • v.38 no.1
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    • pp.160-164
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    • 2020
  • Papenfussiella densa was described as Papenfussiella kuromo f. densa from Japan by Inagaki in 1958. P. densa has been recognized as an endemic and independent species based on the molecular analyses of type material without detailed morphological observations. In this study, Papenfussiella densa is reported as a new record from Dok-do, South Korea, based on morphological and molecular analyses. Papenfussiella densa is mainly characterized as having narrow, branched, slimy, and tomentose thalli with branchlets, partially hollow in the medulla of the middle part. The molecular analyses of the chloroplast rbcL-rbcS DNA sequence of the Papenfussiella densa sample from Korea revealed that it matched that of P. densa from Japan and was nested in the clade of Papenfussiella. There was only a 0.02% gene sequence divergence between the Korean and Japanese samples. We report P. densa as a new record from Korea and add this species to the list of Korean macroalgal flora.

A Newly Recorded Sea Star of the Genus Luidia (Asteroidea: Paxillosida: Luidiidae) from the Korea Strait, Korea

  • Kim, Donghwan;Kim, Minkyung;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.2
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    • pp.131-135
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    • 2017
  • Asteroid specimens of the genus Luidia were collected at a depth of 95-100 m in the Korea Strait by bottom trawling in April 2016. The specimens were identified as Luidia avicularia Fisher, 1913 (Luidiidae: Paxillosida) based on morphological characteristics and molecular phylogenetic analyses, and the species is new to the Korean fauna. A 648-bp partial nucleotide sequence of mitochondrial cytochrome c oxidase I (mt-COI) gene was obtained from Korea, and then was compared to sequences of related species stored in GenBank using molecular phylogenetic analyses. No sequence differences were detected between the L. avicularia mt-COI gene sequences from Korea and China, and the species described in this report was clearly distinct from L. maculata, which was previously reported in Korean fauna. Three Luidia species have been reported in Korea.

Genetic Diversity of Orobanche cumana Populations in Serbia

  • Ivanovic, Zarko;Marisavljevic, Dragana;Marinkovic, Radovan;Mitrovic, Petar;Blagojevic, Jovana;Nikolic, Ivan;Pavlovic, Danijela
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.512-520
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    • 2021
  • In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

Cryptonemia asiatica sp. nov. (Halymeniaceae, Rhodophyta), a new marine macroalgal species from Korea and Japan

  • Yang, Mi Yeon;Kim, Myung Sook
    • Journal of Ecology and Environment
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    • v.37 no.4
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    • pp.387-393
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    • 2014
  • We propose Cryptonemia asiatica sp. nov. from Korea and Japan. We used molecular analyses of plastid-encoded rbcL and morphological observations to resolve the taxonomic identities of C. lactuca from Korea, C. luxurians from Japan, and C. seminervis from Spain. Specimens of C. lactuca and C. luxurians fell within the same molecular phylogenetic clade (with 100% bootstrap support) and were clearly separated from specimens of C. luxurians collected from the type locality in Brazil. Our analyses demonstrated identical molecular sequences between C. seminervis specimens from Spain and C. lomation specimens from France. Morphological characteristics of the new species, C. asiatica include prominent midribs through the mid thallus, a cortex 4-6 cells thick, and a blade with undulate margins. Molecular evidence indicates that specimens from Korea and Japan previously assigned to C. lactuca and C. luxurians, respectively, should be reassigned to Cryptonemia asiatica. Binomial C. luxurians from Brazil should be resurrected as the independent species of Cryptonemia.

New Record of a Sea Urchin Echinometra mathaei (Echinoidea: Camarodonta: Echinometridae) from Jeju Island, Korea and Its Molecular Analysis

  • Lee, Taek-Jun;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.178-184
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    • 2012
  • Echinoids were collected at depths of 5-10 m in Munseom, Jeju Island by SCUBA diving on November 23, 2008 and September 15, 2009. Two specimens were identified as Echinometra mathaei (Blainville, 1825) based on morphological characteristics and molecular analyses of mitochondrial cytochrome c oxidase subunit I partial sequences. Echinometra mathaei collected from Korea was redescribed with photographs and was compared with other species from GenBank based on molecular data. Phylogenetic analyses showed that no significant differences were between base sequences of E. mathaei from Korea and that from GenBank. To date, 13 echinoids including this species have been reported from Jeju Island, and 32 echinoids have been recorded in Korea.

Based on morphology and molecular data, Palisada rigida comb. nov. and Laurencia decussata comb. et stat. nov. (Rhodophyta, Rhodomelaceae) are proposed

  • Metti, Yola
    • ALGAE
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    • v.37 no.1
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    • pp.15-32
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    • 2022
  • Inspecting herbaria collections of Laurencia rigida highlighted frequent misidentifications between L. rigida and L. heteroclada f. decussata, two poorly studied taxa from Australia. Recent collections of DNA material, including from topotype material, allowed for re-examination of these two taxa using molecular techniques. Detailed morphological and molecular analyses based on two markers (rbcL and COI-5P) strongly supported these two taxa as being distinct from each other and requiring nomenclatural changes. Comprehensive morphological analyses highlighted features useful for accurate identifications. Interestingly, L. rigida was found to belong to the genus Palisada with evidence from both the morphology and molecular data. Therefore, this study proposed recognizing L. rigida as Palisada rigida comb. nov. Molecular data for L. heteroclada f. decussata on the other hand supported its separation from L. heteroclada, with too great a molecular distance to be considered a variety. Morphological characters that best separated P. rigida from L. decussata included seven characters; number of pericentral cells per vegetative axial segment, the presence of secondary pit connections, the presence of lenticular thickenings, tetrasporangia alignment, the presence of corps en cerise, holdfast morphology, and overall plant shape. Morphologically, L. heteroclada f. decussata was also separated from L. heteroclada, particularly by the following characteristics; ultimate branchlets morphologies, lower order branch lengths, primary axis and holdfast morphologies. Therefore, it was proposed that L. heteroclada f. decussata is recognized at a species level as L. decussata comb. et stat. nov.

Synthesis of Chiral Poly(norbornene carboxylic acid ester)s and Their Characteristic Properties in The Thin Film

  • Byun, Gwang-Su;Lee, Taek-Joon;Jin, Kyeong-Sik;Ree, Moon-Hor;Kim, Sang-Youl;Cho, I-Whan
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.333-333
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    • 2006
  • We synthesized two novel polynorbornene derivatives, chiral poly(norbornene acid methyl ester) (C-PNME) and racemic poly(norbornene acid n-butyl ester) (R-PNME), which are potential low dielectric constant materials for applications in advanced microelectronic and display devices. Thin films of these polymers deposited on substrates were investigated by structural analyses using synchrotron grazing incidence X-ray scattering, specular reflectivity and ellipsometry. These analyses provided important information on the structure, electron density gradient across film thickness, chain orientation, refractive index and thermal expansion of the polymers in substrate-supported thin films. The structural characteristics and properties of the thin films were first dependent on the polymer chain' tacticity and further influenced by film thickness and thermal annealing.

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