• Title/Summary/Keyword: moisture supply

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Characterization of Protein Function and Differential Protein Expression in Soybean under Soaking Condition (Proteomics를 이용한 콩의 발아 전 침종처리에 따른 단백질 발현 양상 비교 분석)

  • Cho, Seong-Woo;Kim, Tae-Sun;Kwon, Soo-Jeong;Roy, Swapan Kumar;Lee, Chul-Won;Kim, Hong-Sig;Woo, Sun-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.1
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    • pp.114-122
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    • 2015
  • Soybean is very useful crop to supply vegetable protein for human. However, cultivation arear of this economically important crop is gradually diminished in upland field. Hence, cultivation area of soybean is increased in paddy field. During the growth duration of soybean, excessive moisture injury is serious problem for sustainable production and supply. We investigated protein expression according to different period of seed soaking and germination after seed soaking. For comparison on expression of protein according to different condition, we performed two-dimensional electrophoresis. After electrophoresis analysis, we selected differentially expressed protein spots according to different condition such as soaking period and germination after soaking to identify protein function by using MALDI-TOF. Results revealed that pattern of expression of protein according to soaking period and germination after soaking were generally not different in major spots. However, degree of expression of protein in some protein spots was increased in accordance with decrease of soaking period. Especially, in Hwangkeum-Kong, Danyeop-Kon, and Pecking, the degree of expression of protein was remarkably increased for 4 days after soaking. But, according to germination after soaking, degree of expression of protein in germinated seeds of all cultivars was higher than un-germinated seeds. In results of MALDI-TOF analysis, specific proteins were identified by different soaking period such as Allergen Gly m Bd 28K, P24 oleosin isoform B. Also, in accordance with germination, degree of protein expression of the related protein, Gibberellin was increased in un-germinated seeds of Iksan-Kong. In ungerminated seeds of Sinpaldal-kong, proteins were identified as down-regulated by soaking such as ATP binding and Inhibitor II', proteinase.

A Study on the Characteristics Using Pig Manure Under Aerobic Air Flow Rate During Composting (돈분이용 퇴비화과정에서의 공기공급량별 퇴비화 특성변화에 관한 연구)

  • Kwag, J.H.;Kim, J.H.;Jeong, K.H.;Cho, S.H.;Ahn, H.K.;Choi, D.Y.;Jeong, M.S.;Lee, S.C.;Kang, H.S.;Ra, C.S.
    • Journal of Animal Environmental Science
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    • v.17 no.2
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    • pp.131-138
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    • 2011
  • This study was carried out to investigate on the composting characteristics variation accoding to air supply capacity in Pig manure. The composting of pig manure is economical and efficiently process. The fermented compost was added in pig manure mixed with sawdust was composting reators. Air supply capacity levels of fermented compost on the pig manure mixed with sawdust were regulated at 50, 100, 150 and 200$\ell/m^3$/min. respectively. The obtained results can be followed as bellow; The temperature variations of experimental composting piles during composting for the different of T-1 reach $40^{\circ}C$ in 2 days, T-2, T-3 and T-4 reach $60^{\circ}C$ in 2 days and T-3, T-4 maintained until 8 days. The decreases in water contents per each square meter for the different of T-1 (50 l/$m^3$/min), T-2(100 l/$m^3$/min), T-3(150 l/$m^3$/min) and T-4(200 l/$m^3$/min.), The decreases ratio in water contents was T-1, T-2, T-3 and T-4 were 15.4%, 28.8%, 33.4% and 35.2%. The decreases ratio in weight was T-1, T-2, T-3 and T-4 were 7.6%, 15.6%, 16.8% and 16.9% respectively. The variations of oxygen concentration from composting period in case of oxygen discharge concentration T-1, T-2, T-3 and T-4 were 9 ppm. respectively. Fertilizer components after composting were examined. Nitrogen contents of the T-1, T-2, T-3 and T-4 were 0.45%, 0.44%, 0.42% and 0.44%, and P2O5 contents were T-1, T-2, T-3 and T-4 were 0.37%, 0.41%, 0.42% and 0.44% respectively. Therefore, the compost curing air supply of air volumes at least 150$\ell$/min/min. or more to supply the aerobic composting pig manure normally are judged to be possible.

Influences of Mixing Frequency on the Composting Performance of the Broiler and Dairy Manure Mixtures (퇴비재료의 교반빈도가 육계분과 유우분 혼합물 퇴비화 성능에 미치는 영향)

  • Park K.J.;Bae Y.H.;Hong J.H.;Wi T.W.
    • Journal of Animal Environmental Science
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    • v.12 no.1
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    • pp.41-44
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    • 2006
  • Broiler manure has much nutrient that can be used as the organic fertilizer to enhance the fertility of soil. However, if it is used directly without biodegradation of organic materials and destruction of weed seed and harmful bacteria, it can produce the generation of weed and disease of plant. Composting of manure is a biodegradation of organic materials into inorganic materials and humus. To proceed biodegradation of manure effectively and enhance the composting performance, optimum environmental condition for microbial growing should be maintained. Environmental variables which can influence the growing activity of microbes are moisture content, pH, porosity, C/N of the composting materials and oxygen supply quantity. Oxygen and porosity are usually supplied by aeration or mixing of materials. This study was intended to investigate the effect of mixing frequency on the composting performance. Mixing of composting materials was performed by turning the bioreactor up and down by hand without any mechanical energy. The broiler manure was captured from the greenhouse type broiler ham as the compounds of broiler manure and rice-hulls, which were used as the base materials. To compost the compounds of broiler manure and rice-hulls, dairy manure was mixed to get appropriate characteristics of composting material. Composting temperature over $55^{\circ}C$ for killing pathogen and weed seed was maintained for longer period with increase of mixing frequency.

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Environment-Friendly Metal Cutting Technology using Cooled Air (냉각공기에 의한 환경 친화적 절삭가공기술)

  • Lee, Jong-Hang;Cho, Woong-Shik;Chung, Joon-Ki;Park, Ceol-Woo;Kim, Young-Jung
    • Journal of the Korean Society for Precision Engineering
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    • v.18 no.6
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    • pp.114-120
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    • 2001
  • It is necessary to develop a new metal cutting technology which does not use cutting fluid, since cutting fluid can have undesirable effect on workers's health and working environment. For this to be possible, it is necessary to replace the conventional method of using cutting fluid, whose basic functions are removing chip and heat, and providing lubrication between tool and chip. In this work, cooled air is utilized in order to replace cutting fluid. Experiments were carried out while cutting workpiece with HSS flat endmill under a variety of supply conditions for cooled air. Also the performance characteristics of the air cooling system. which was built for the experiments, were carefully analyzed. For the reliable operation of air cooling system. moisture contained in the cooled air had to be removed before being supplied to the workpiece and tools. It was found that depending on the amount of its flow rate the temperature of cooled air changes at the time of injection from the nozzle. The flow rate of cooled air also plays an important role in removing the accumulated chip on the workpiece. After comparing the flank wear for the three cases of using cooled air, cutting fluid, and pure dry technique, it was demonstrated that the level of flank wear was similar for the cases of cooled air and cutting fluid. The pure dry technique, however, showed higher level of flank wear than cooled air.

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Characteristics of Icing Phenomenon with Droplet of an Injector for Liquid Phase LPG Injection System (LPG 액상분사식 인젝터에서 후적에 의한 아이싱 특성 연구)

  • Park, Cheol-Woong;Kim, Chang-Up;Choi, Kyo-Nam;Kang, Kern-Yong
    • Transactions of the Korean Society of Automotive Engineers
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    • v.15 no.5
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    • pp.9-16
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    • 2007
  • Since the liquid phase LPG injection(LPLI) system has an advantage of higher power and lower emission characteristics than the mixer type fuel supply system, many studies and applications have been conducted. However, the heat extraction, due to the evaporation of liquid fuel, causes not only a dropping of LPG fuel but also icing phenomenon that is a frost of moisture in the air around the nozzle tip. Because both lead to a difficulty in the control of accurate air fuel ratio, it can result in poor engine performance and a large amount of HC emissions. The experimental investigation was carried out on the bench test rig in this study. It was found that n-butane, that has a relatively high boiling point($-0.5^{\circ}C$), was a main species of droplet composition and also found that the droplet problem was improved by the use of a large inner to outer bore ratio nozzle whose surface roughness is smooth. The icing phenomena were decreased when the an engine head temperature was increased, although a large amount of icing deposit was still observed in the case of $87^{\circ}C$. Also, it was observed that the icing phenomenon is improved by using anti-icing bushing.

Evaluation of Nondestructive Diagnosis and Material Characteristics of Stone Lantern at Damyang Gaeseonsaji Temple Site in Korea

  • Lee, Chan Hee;Araki, Naruto
    • Journal of Conservation Science
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    • v.35 no.4
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    • pp.279-293
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    • 2019
  • The stone lantern of the Damyang Gaeseonsaji temple site is a cultural heritage built during the Unified Silla period (AD 868). The reason for its value as a cultural property is due to wittern the background and the period created on inscription of the lamp stone engraved by letters. The stone lantern consists of two types of lithic tuffs for the 23 original properties, the replaced stones in 1991, and the biotite granite for its ground stones replaced in 2005. The lithic tuffs selected as the replacement parts in 1991 and 2017 have been examined and got to properties of hardly exposure moisture as well as very similar geochemical characteristics. There were various types of physical deterioration of the stone properties and structural cracks; in particular, on the northern side of the stylobates. Chemical and biological deterioration can be identified as black, white, and brown discolorations as well as by the presence of lichens, bryophytes, and herbaceous plants. In the evaluation of the physical properties of the stone lantern, the mean and maximum ultrasonic velocities were found to be similar in each direction. However, the lowest velocity on the east and south sides were found to be lower than those of other stone properties. It was found that physical damage to the stylobates resulted from water expansion in a freeze-to-thaw phenomena related to water content. Therefore, dismantling repair was carried out in the protection facility to restrict further water supply to the stone as much as possible.

Basic Study on Oversea Biomass Energy Resources 1 - Palm Biomass (해외 바이오매스 에너지자원 확보를 위한 기초조사 1 - 팜 바이오매스)

  • Lee, Hyoung Woo
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.4
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    • pp.439-449
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    • 2014
  • RPS (Renewable Portfolio Standard) has increased wood pellet demand dramatically in recent years in Korea where self-supply rate of wood pellet is not more than 10%. However global production capacity of wood pellet is prospected to be unable to meet the global demand after 2020. Therefore it is urgently needed to develop new sustainable biomass energy resources which can replace wood pellet at lower cost. As a result of this study EFB (empty fruit bunch) and MF (mesocarp fiber), the representative solid palm biomass, are estimated to be generated at the rate of 20 and 28 million tons per year (based on 10% moisture content) in Malaysia and Indonesia, respectively in 2012. Total annual generation rate of EFB and MF is estimated as 48 million tons per year only in Malaysia and Indonesia in 2012. With calorific value of over 90% of wood pellet EFB is expected to be a excellent biomass energy resource which can replace wood pellet. EFB can be utilized as fuel for power generation or industrial purpose. However EFB may not be a proper fuel for domestic and greenhouse heating because of its high ash content.

Development of a Portable Quality Evaluation System for Bee-honeys by Using Near Infrared Spectroscopy (근적외 분광법을 응용한 휴대용 벌꿀 품질 평가 장치 개발)

  • Choi, Chang-Hyun;Kim, Jong-Hun;Kwon, Ki-Hyun;Kim, Yong-Joo
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.156-164
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    • 2011
  • This study was conducted to develop a portable quality evaluation system of bee-honey by near infrared spectroscopic technique. Two kinds of bee-honeys from acacia and polyflower sources were tested in this study. The system consists of power supply, tungsten-halogen lamp, detector, and optical fiber probe. Performance of the system was analyzed by comparing the prediction accuracy of the laboratory spectrophotometer. Total of 346 spectra was divided into a calibration set and a prediction set. The PLS (Partial Least Squares) models were developed to predict the quality parameters of bee-honeys. Reflectance spectra, moisture contents, ash, invert sugar, sucrose, F/G ratio, HMF(hydroxy methyl furfural), and $C^{12}/C^{13}$ ratio of honeys were measured. The PLS models of the laboratory spectrophotometer showed good relationships between predicted and measured quality parameters of honeys in the wavelength range of 1.100~2.200 nm. The PLS analysis of the portable quality evaluation system showed good relationships between predicted and measured quality parameters of honeys in the wavelength range of 1.100~1.300 nm and 1.400~1.700 nm. The results showed the feasibility of the portable quality evaluation system to determine the quality parameters of bee-honey in the field during harvesting.

A Comparative Study on Theories of Optical Disease Based on Si-sheng-xin-yuan and Eui-gam-jung-ma (<사성심원>과 <의감중마>의 안병이론에 관한 비교 고찰)

  • Lee Sang man;Eun Hyun Sup;Chi Gyoo Yong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1141-1146
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    • 2003
  • In order to investigate different and original theories on pathology of eyes from the past main etiology of fire and heat, two texts of Si-sheng-xin-yuan written by Huang Yuan Yu and Eui-gam-jung-ma written by Lee Gyu Jun are selected and analysed in terms of pathology and prescription. Huang explained that diseases of the eyes are usually born of functional disorders of spleen and stomach(脾胃升降失調) accompanied with turbidity change of energy and blood(氣血淸濁變化). In the meantime, Lee described that the diseases are made from disorders of activities of essence, spirit, energy and blood stored in 5 viscera. So following them, the main point of treatment for the diseases of eyes is to restore and clarify the stagnated gastrointestinal(GI) function, or to supply the clear essence and blood to eyes respectively. Also they have same opinions that the fire and heat are the secondary symptoms of the optical diseases. Therefore Huang focused on cleaning the phlegm and leaking the moisture of GI tract to treat those symptoms, and Lee emphasized on nourishing essential energy of kidney and liver on the other hand. Although they preferred to use radical therapy than symptomatic one. But it can be deduced that Huang's theory is more positive and direct therapy and Lee's one is more basic but indirect treatment.

An Evaluation on Suitability of Drought Indices with ROC Space (ROC Space를 통한 가뭄지수의 적합성 평가)

  • Kim, Gwang-Seob;Lee, Jun-Won
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.123-123
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    • 2011
  • 가뭄은 다른 기상재해들과 달리 특정한 기후현상에 의해 발생하는 사건이 아닌 장기간의 강우 부족으로 인한 물 부족으로부터 기인하며, 가뭄의 특성상 가뭄의 시작과 끝을 명확히 구분하기 힘들며, 심도를 결정짓는 것 또한 어려움이 있다. 이러한 가뭄의 특성을 파악하기 위한 연구는 계속되고 있으며, SPI(Standardized Precipitation Index), PDSI(Palmer Drought Severity Index), SWSI(Surface Water Supply Index), EDI(Effective Drought Index), CMI(Crop Moisture Index)등과 같은 가뭄의 특성을 잘 반영한 가뭄지수의 개발 또한 끊임없이 이어지고 있다. 하지만 이러한 가뭄지수들은 기상학적, 기후학적, 농업적, 수문학적등과 같은 분류에 의해 가뭄의 표현이 상이한 결과를 보여주며, 동일한 방법으로 산정된 가뭄평가지수라 하더라도 지역적인 적합성 정도에서 또한 차이를 보인다. 본 연구에서는 우리나라의 실제 가뭄의 발생사례를 바탕으로 각종 가뭄지수들의 적합도와 가뭄의 변동특성을 파악하고자 한다. 우리나라의 가뭄특성을 확인하기 위하여 보고서 등 각종 문헌과 신문기사를 통해 1973년부터 2009년까지 실제 가뭄발생 기록을 정량화하고 행정구역단위의 우리나라 전역에 공간분포로 표현하였다. 69개 기상관측소의 강수 및 기온 자료를 통해 기상청과(SPI, PDSI, PN, 강수량십분위) 동일한 방법으로 가뭄지수를 산정 후 마찬가지로 행정구역단위의 우리나라 전역에 확장하였으며, 이렇게 생성된 각종 가뭄지수 및 기후변수의 공간분포와 실제 가뭄발생사례의 공간분포를 비교 분석함으로서 각 가뭄지수 및 기후변수의 적합성을 평가하였다. 각 가뭄지수 및 기후변수의 적합성을 평가하기 위하여 ROC space 상의 검정통계량을 이용하였다. 분석결과 PN(Percent of Normal)이 실제 가뭄의 현상을 가장 잘 표현했으며, 강수량, SPI 3, 강수량 십분위 등이 높은 상관성을 보였다. 또한 SPI12, PDSI, PN, 강수량십분위 등이 행정구역에 따른 산포정도가 비교적 낮게 나타났다. 본 연구를 통해 우리나라 전 지역 가뭄의 시 공간적인 가뭄변동특성을 파악하고, 기존에 사용되고 있는 가뭄지수의 적합도 평가를 통해 우리나라 가뭄특성을 가장 잘 반영한 가뭄지수의 선정과 각종 기후특성을 잘 반영하는 좀 더 향상된 가뭄지수 개발에 도움을 줄 수 있으며, 가뭄의 시 공간적인 예측에 대해 적합한 가뭄지수 선택에 도움을 줄 것으로 판단된다.

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