• Animal Bioscience
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• v.36 no.8
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• pp.1285-1292
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• 2023

Objective: The objective of this study was to develop a novel endolysin (PanLys.1) for the specific killing of the ruminal hyper-ammonia-producing bacterium Peptostreptococcus anaerobius (P. anaerobius). Methods: Whole genome sequences of P. anaerobius strains and related bacteriophages were collected from the National Center for Biotechnology Information database, and the candidate gene for PanLys.1 was isolated based on amino acid sequences and conserved domain database (CDD) analysis. The gene was overexpressed using a pET system in Escherichia coli BL21 (DE3). The lytic activity of PanLys.1 was evaluated under various conditions (dosage, pH, temperature, NaCl, and metal ions) to determine the optimal lytic activity conditions. Finally, the killing activity of PanLys.1 against P. anaerobius was confirmed using an in vitro rumen fermentation system. Results: CDD analysis showed that PanLys.1 has a modular design with a catalytic domain, amidase-2, at the N-terminal, and a cell wall binding domain, from the CW-7 superfamily, at the C-terminal. The lytic activity of PanLys.1 against P. anaerobius was the highest at pH 8.0 (p<0.05) and was maintained at 37℃ to 45℃, and 0 to 250 mM NaCl. The activity of PanLys.1 significantly decreased (p<0.05) after Mn²⁺ or Zn²⁺ treatment. The relative abundance of P. anaerobius did not decrease after administration PanLys.1 under in vitro rumen conditions. Conclusion: The application of PanLys.1 to modulate P. anaerobius in the rumen might not be feasible because its lytic activity was not observed in in vitro rumen system.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.6
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• pp.26-37
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• 2008

The purpose of the study was to evaluate the effect of pervious pavements on reducing the surface runoff caused by rainfall. The surface runoff from twelve steel experimental beds with different pavement had been recorded every minute from May to September 2008, by the measuring system of tipping buckets(0.1mm/count) and data aquisition systems(National Instrument's Labview and DAQ boards & Autonics PR12-4). The dimension of the experimental bed was $1.5m(W){\times}2.0m(L){\times}0.6m(D)$ and eleven different kinds of vegetational(grass, grass+cubic stone, grass+hole brick), modular(brick, cubic stone, small cubic stone, wood block, interlocking block, clay brick, granular clay brick) and granular(naked soil, gravel) paving materials and concrete were applied for the comparison. Six rain events with depth over 30mm were selected and compared. The maximum depth of the rainfall selected was 137.5mm for 28 hours, and the minimum 30mm for 5 hours. The maximum rainfall per hour was 23mm/hr and the minimum 11.4mm/hr. The major findings were as follows; 1. All pervious pavement applied reduced over 75% of the surface runoff compared with concrete pavement. The grassy and porous pavements were relatively efficient in reducing surface runoff. 2. The grass was the more efficient as intercepting average 69.5mm of initial surface runoff, and maximum 77.8mm at the condition of 13.5mm/hr rainfall. The next was gravel intercepting maximum 65.5mm at the condition of 13.5mm/hr and the 40.9mm at 19.1mm/hr, average 55.7mm. 3. The modular pavements common in urban area were not good in intercepting the runoff except the 'clay granular brick' compared with others. The 'clay granular brick' showed relatively efficient intercepting average 14.1mm, which was the bigger amount than the 'grass+hole brick'. 4. The 'naked soil' were more effective than the 'concrete', 'brick', and 'interlocking block' in reducing the surface runoff, but less efficient than other materials. The capacity of the 'naked soil' to intercept the initial rainfall was similar to the 'brick'. As summary, the more grassy and porous pavement shows more effective in reducing surface runoffs.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.503-511
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• 2013

The purpose of this study was to evaluate temperature reduction and heat budget of extensive modular green roof planted with Sedum sarmentosum and Zoysia japonica. Plant height and green coverage were measured as plant growth. Temperature, net radiation and evapotranspiration of concrete surface, green roof surface, in-soil and bottom were measured from August 2 to August 3, 2012 (48 hours). On 3 P.M., August 3, 2012, when air temperature was the highest ($34.6^{\circ}C$), concrete surface temperature was highest ($57.5^{\circ}C$), followed by surface temperature of Sedum sarmentosum ($40.1^{\circ}C$) and Zoysia japonica ($38.3^{\circ}C$), which proved temperature reduction effect of green roof. Temperature reduction effect of green roof was also shown inside green roof soil, and bottom of green roof. It was found that Zoysia japonica was more effective in temperature reduction than Sedum sarmentosum. Compared with the case of concrete surface, the highest temperature of green roof surface was observed approximately 2 hours delayed. Plant species, temperature and soil moisture were found to have impact on surface temperature reduction. Plant species, air temperature, soil moisture and green roof surface temperature were found to have impact on temperature reduction in green roof bottom. As results of heat budget analysis, sensible heat was highest on concrete surface and was found to be reduced by green roof. Latent heat flux of Zoysia japonica was higher than that of Sedum sarmentosum, which implied that Zoysia japonica was more effective to improve thermal environment for green roof than Sedum sarmentosum.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.32 no.5
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• pp.333-340
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• 2019

Tests using a middle velocity propulsion impact machine (MVPIM) were performed to verify the impact resistance capability of sandwich concrete panels (SCP) in a modular liquefied natural gas (LNG) outer tank, and numerical models were constructed and analyzed. $2{\times}2m$ specimens with plain sectional characteristics and specimens including a joint section were used. A 51 kg missile was accelerated above 45 m/s and impacted to have the design code kinetic energy. Impact tests were performed twice according to the design code and once for the doubled impact speed. The numerical models for simulating impact behaviors were created by LS-DYNA. The external steel plate and filled concrete of the panel were modeled as solid elements, the studs as beam elements, and the steel plates as elasto-plastic material with fractures; the CSCM material model was used for concrete. The front plate deformations demonstrated good agreement with those of other tests. However the rear plate deformations were less. In the doubled speed test for the plain section specimen, the missile punctured both plates; however, the front plate was only fractured in the numerical analysis. The impact energy of the missile was transferred to the filled concrete in the numerical analysis.

• KIPS Transactions on Software and Data Engineering
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• v.11 no.7
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• pp.299-306
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• 2022

In recent, sensors embedded in robots, equipment, and circuits have become common, and research for diagnosing device failures by learning measured sensor data is being actively conducted. This failure diagnosis study is divided into a classification model for predicting failure situations or types and a regression model for numerically predicting failure conditions. In the case of a classification model, it simply checks the presence or absence of a failure or defect (Class), whereas a regression model has a higher learning difficulty because it has to predict one value among countless numbers. So, the reason that regression modeling is more difficult is that there are many irregular situations in which it is difficult to determine one output from a similar input when predicting by matching input and output. Therefore, in this paper, we focus on input and output data with periodicity, analyze the input/output relationship, and secure regularity between input and output data by performing sliding window-based input data patterning. In order to apply the proposed method, in this study, current and temperature data with periodicity were collected from MMC(Modular Multilevel Converter) circuit system and learning was carried out using ANN. As a result of the experiment, it was confirmed that when a window of 2% or more of one cycle was applied, performance of 97% or more of fit could be secured.

• Nuclear Engineering and Technology
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• v.54 no.12
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• pp.4571-4584
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• 2022

The analysis of rapid flow transients in Reactor Coolant Pumps (RCP) is essential for a reactor safety study. An accurate and precise analysis of the RCP coastdown is necessary for the reactor design. The coastdown of RCP affects the coolant temperature and the colloidal crud in the primary coolant. A realistic and kinetic model has been used to investigate the behavior of activated colloidal crud in the primary coolant and steam generator that solves the pump speed analytically. The analytic solution of the non-dimensional flow rate has been determined by the energy ratio β. The kinetic energy of the coolant fluid and the kinetic energy stored in the rotating parts of a pump are two essential parameters in the form of β. Under normal operation, the pump's speed and moment of inertia are constant. However, in a coastdown situation, kinetic damping in the interval has been implemented. A dynamic model ACCP-SMART has been developed for System Integrated Modular and Advanced Reactor (SMART) to investigate the corrosion due to activated colloidal crud. The Fickian diffusion model has been implemented as the reference corrosion model for the constituent component of the primary loop of the SMART reactor. The activated colloidal crud activity in the primary coolant and steam generator of the SMART reactor has been studied for different equilibrium corrosion rates, linear increase in corrosion rate, and dynamic RCP coastdown situation energy ratio b. The coolant specific activity of SMART reactor equilibrium corrosion (4.0 mg s^-1) has been found 9.63×10^-3 µCi cm^-3, 3.53×10^-3 µC cm^-3, 2.39×10^-2 µC cm^-3, 8.10×10^-3 µC cm^-3, 6.77× 10^-3 µC cm^-3, 4.95×10^-4 µC cm^-3, 1.19×10^-3 µC cm^-3, and 7.87×10^-4 µC cm^-3 for ²⁴Na, ⁵⁴Mn, ⁵⁶Mn, ⁵⁹Fe, ⁵⁸Co, ⁶⁰Co, ⁹⁹Mo, and ⁵¹Cr which are 14.95%, 5.48%, 37.08%, 12.57%, 10.51%, 0.77%, 18.50%, and 0.12% respectively. For linear and exponential coastdown with a constant corrosion rate, the total coolant and steam generator activity approaches a higher saturation value than the normal values. The coolant and steam generator activity changes considerably with kinetic corrosion rate, equilibrium corrosion, growth of corrosion rate (ΔC/Δt), and RCP coastdown situations. The effect of the RCP coastdown on the specific activity of the steam generators is smeared by linearly rising corrosion rates, equilibrium corrosion, and rapid coasting down of the RCP. However, the time taken to reach the saturation activity is also influenced by the slope of corrosion rate, coastdown situation, equilibrium corrosion rate, and energy ratio β.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.4
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• pp.512-516
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• 2004

Context-aware computing has been attracting the attention as an approach to alleviating the inconvenience in human-computer interaction. This paper proposes a context-aware system architecture to be implemented on an SoC(System-on-a-Chip). The proposed architecture supports sensor abstraction, notification mechanism for context changes, modular development, easy service composition using if-then rules, and flexible context-aware service implementation. It consists of the communication unit, the processing unit, the blackboard, and the rule-based system unit, where the first three components reside in the microprocessor part of the SoC and the rule-based system unit is implemented in hardware. For the proposed architecture, an SoC system has been designed and tested in an SoC development platform called SystemC and the feasibility of the behavoir modules for the microprocessor part has been evaluated by implementing software modules on the conventional computer platform. This SoC-based context-aware system architecture has been developed to apply to mobile intelligent robots which would assist old people at home in a context-aware manner.

• Journal of Wetlands Research
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• v.13 no.3
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• pp.593-602
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• 2011

The estuarine area of the ecological environment had been seriously damaged by reckless developments during the past half century. The demand for management in estuarine area increased but the efficient management system did not exist. Therefore the aim of this study is to develop a GIS-based integrated DB management system by integrating the ecological environmental data of each division in estuarine area. To achieve this, the system used integrated DB. It is classified into a surveyed monitoring DB in 2010 at estuarine area of Yeongsan and GIS DB to express the spatial data. The integrated DB management system was developed using VisualBasic.NET languages and Arcobjects component for Map-based spatial analysis. Also, to improve the utilization of data, the composition of GUI(Graphical User Interface) through user needs analysis, the expression of monitoring DB, the priority of layer and the function for modular unit were defined. Ultimately the system was developed based on the defined items. GIS-based integrated DB management system can support to understand the ecological environment in estuarine area of Yeongsan river. Also it will provide the users with effective sharing and environmental management of the data. In the future study, comprehensive diagnosis for ecological environment in estuarine area and the reliable prediction need to be made by connecting modeling system.

• Journal of Korean Society of Forest Science
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• v.102 no.1
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• pp.30-37
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• 2013

Analysis of new approaches to achieve naturally good ecological potential of forest road cut-slope by making the best use of advantages of gabion systems with vegetation base materials to prevent slope failure and erosion, in the area with highly erodible soil. Existing gabion systems can be divided into monolithic and modular system and can be divided into ten subtypes according to the purpose of establishment and combination of other measures. As a result on the monitoring of erosion amount from forest road cut-slopes in the test applications, the order of erosion amount from largest to smallest is as follows: the curved road cut-slope site where normal gabion system was established 5,840 $cm^3$; the control site 5,833 $cm^3$; the straight road cut-slope site where normal gabion system was established 5,621 $cm^3$; the curved road cut-slope site where the new gabion system was established 4,298 $cm^3$; and the straight road cut-slope site where the new gabion system 4,117 $cm^3$. Therefore, the result shows that the new gabion system is more effective than the normal gabion system to reduce erosion amount from forest road cut-slopes. During the study period, vegetation coverages of the straight and curved road cut-slope site where the new gabion system was established were about 56(30~85)% and about 45(28~65)%, so average vegetation coverage of the sites where the new gabion systems was established was higher than the sites where the normal gabion systems was established. Therefore, it was concluded that the new gabion system can be more effective for cut-slope revegetation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.10
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• pp.1861-1871
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• 1994

This paper presents efficient real time software implementation methods for the grayscale morphological composite function processing (FP) system. The proposed method is based on a matrix representation of the composite FP system using a basis matrix composed of structuring elements. We propose a procedure to derive the basis matrix for composite FP systems with any grayscale structuring element (GSE). It is shown that composite FP operations including morphological opening and closing are more efficiently accomplished by a local matrix operation with the basis matrix rather than cascade operations, eliminating delays and requiring less memory storage. In the second part of this paper, a VLSI implementation architecture for grayscale morphological operators is presented. The proposed implementation architecture employs a bit-serial approach which allows grayscale morphological operations to be decomposed into bit-level binary operation unit for the p-bit grayscale singnal. It is shown that this realization is simple and modular structure and thus is suitable for VLSI implementation.