• Journal of Embryo Transfer
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• v.33 no.4
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• pp.271-280
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• 2018

Here, we evaluated the mode of programmed cell death during porcine oocyte maturation by comparing the two major pathways associated with programmed cell death, apoptosis (type I), and autophagy (type II). We investigated the expression and localization of major genes involved in autophagy and apoptosis at mRNA and protein levels. Furthermore, the effect of hormonal stimulation on autophagy and apoptosis was analyzed. We found that the activity of autophagy-associated genes was increased in the cumulus-oocyte complexes (COCs) following follicle-stimulating hormone (FSH) treatment, while the addition of luteinizing hormone (LH) reversed this effect. The expression of proteins associated with autophagy was the highest in FSH-treated COCs. On the other hand, caspase-3 protein level was maximum in COCs cultured with LH. The treatment with rapamycin resulted in the effect similar to that observed with FSH treatment and increased autophagy activity. Thus, hormonal stimulation of pig oocytes resulted in distinct patterns of maturation. The high-quality oocytes majorly relied on the type II pathway (autophagy), while the type I pathway (apoptosis) was more prominent among poor-quality oocytes. Further investigation of this distinction may allow the development of techniques to produce high-quality oocytes in porcine in vitro fertilization.

• Computers and Concrete
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• v.27 no.1
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• pp.29-39
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• 2021

This study aimed to develop a method for the determination of the damaged story in reinforced concrete (RC) structure with ambient vibrations, based on modified jerk energy methodology. The damage was taken as a localized reduction in the stiffness of the structural member. For loading, random white noise excitation was used, and dynamic responses from the finite element model (FEM) of 4 story RC shear frame were extracted at nodal points. The data thus obtained from the structure was used in the damage detection and localization algorithm. In the structure, two damage configurations have been introduced. In the first configuration, damage to the structure was artificially caused by a local reduction in the modulus of elasticity. In the second configuration, the damage was caused, using the Elcentro1940 and Kashmir2005 earthquakes in real-time history. The damage was successfully detected if the frequency drop was greater than 5% and the mode shape correlation remained less than 0.8. The results of the damage were also compared to the performance criteria developed in the Seismostruct software. It is demonstrated that the proposed algorithm has effectively detected the existence of the damage and can locate the damaged story for multiple damage scenarios in the RC structure.

• Journal of Korea Multimedia Society
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• v.25 no.5
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• pp.730-738
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• 2022

Based on visual Thinking theory, VR(virtual reality) game changes the traditional form of memory and maps the content into game elements to realize the immersive spatial memory mode. This paper analyzes the influencing factors of game design and system function construction. This paper proposes a hypothesis: with the help of visual thinking theory, VR game is helpful to improve learners' visual memory, and carries out research. The experiment sets different levels of game through empirical research and case analysis of memory flip game. For example, when judging two random cards. If the pictures are the same, it will be judged as the correct combination; if they are different, the two cards will be restored to the original state. The results are analyzed by descriptive statistical analysis and AMOS data analysis. The results show that game content using the concept of "Memory Palace", which can improve the accuracy of memory. We conclude that the use of spatial localization characteristics in flip games combining visual thinking can improve users' memory by helping users memorize and organize information in a Virtual environment, which means VR games have strong feasibility and effectiveness in improving memory.

• Computers and Concrete
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• v.31 no.2
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• pp.151-161
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• 2023

This paper presents an investigation of variables that cause spurious cracking in numerical modeling of concrete fracture. Spurious cracks appear due to the approximate nature of numerical modeling. They overestimate the dissipated energy, leading to divergent results with mesh refinement. This paper is limited to quasi-static loading regime, homogeneous models, cracking as the only nonlinear mode of deformation and cracking only due to tensile loading. Under these conditions, some variables that can be related to spurious cracking are: mesh alignment, ductility, crack band width, structure size, mesh refinement and load increment size. Case studies illustrate the effect of each variable and convergence analyses demonstrate that, after all, load-increment size is the most important variable. Theoretically, a sufficiently small load increment is able to eliminate or at least alleviate the detrimental influence of the other variables. Such load-increment size might be prohibitively small, rendering the simulation unfeasible. Hence, this paper proposes two alternatives. First, it is proposed an algorithm that automatically find such small load increment size automatically, which not necessarily avoid large computations. Then, it is proposed a double simulation technique, in which the crack is forced to propagate through the localization zone.

• Earthquakes and Structures
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• v.19 no.1
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• pp.29-44
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• 2020

The effectiveness and the sensitivity of a Wireless impedance/Admittance Monitoring System (WiAMS) for the prompt damage diagnosis of two single-storey single-span Reinforced Concrete (RC) frames under cyclic loading is experimentally investigated. The geometrical and the reinforcement characteristics of the RC structural members of the frames represent typical old RC frame structure without consideration of seismic design criteria. The columns of the frames are vulnerable to shear failure under lateral load due to their low height-to-depth ratio and insufficient transverse reinforcement. The proposed Structural Health Monitoring (SHM) system comprises of specially manufactured autonomous portable devices that acquire the in-situ voltage frequency responses of a network of twenty piezoelectric transducers mounted to the RC frames. Measurements of external and internal small-sized piezoelectric patches are utilized for damage localization and assessment at various and increased damage levels as the magnitude of the imposed lateral cycle deformations increases. A bare RC frame and a strengthened one using a pair of steel crossed tension-ties (X-bracing) have been tested in order to check the sensitivity of the developed WiAMS in different structural conditions since crack propagation, damage locations and failure mode of the examined frames vary. Indeed, the imposed loading caused brittle shear failure to the column of the bare frame and the formation of plastic hinges at the beam ends of the X-braced frame. Test results highlighted the ability of the proposed SHM to identify incipient damages due to concrete cracking and steel yielding since promising early indication of the forthcoming critical failures before any visible sign has been obtained.

• The Korean Journal of Nuclear Medicine
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• v.25 no.2
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• pp.259-265
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• 1991

The purpose of this study is to investigate the ability of I-131 labeled polyclonal human immunoglobulin to localize an infection. In our country, indium-111 labeled leukocyte or Tc-99m labeled IgG are not readily available because of compex, time-consuming procedure and cost. So we tried to localize infection with I-131 labeled IgG which could be easily prepared. Six rats, infected with staphylococcus aureus in a thigh muscle, received I-131 labeled IgG intravenously and I-131 labeled bovine serum albumin (BSA) were injected to other 5 infected rats. Scintigrams were made at 1, 4, 24, 48, 72 hour later. The radiopharmaceutical demonstrated significant accumulation at the site of infection. The accumulation of I-131 labeled IgG at the site of infection was significantly (P<0.05) higher than that of I-131 labeled BSA at 48, 72 hour. Similar finding could be found at 24 hour imaging, but it was not significant statistically. Therefore it was found that vascular permeability alone could not account for the mode of action of I-131 labeled IgG and it was considered that specific binding played a role. In conclusion, focal sites of inflammation can be detected with I-131 labeled nonspecific human polyclonal IgG and it seems that this method can also be applied to localization of human infection.

• Journal of Chest Surgery
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• v.11 no.2
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• pp.129-134
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• 1978

Two hundred and seventeen patients underwent diagnostic rigid bronchoscopy or bionchofiberscopy to evaluate the cytologic diagnosis in the lung cancer patient at the department of chest surgery of Yon-Sei university, college of the medicine from 1971 to 1977 year. One hundred and twenty cases of these patients were taken rigid bronchoscopy and ninety four cases of these patient were taken bronchofiberscopy. Cytologic examination of the sputum was done in 214 cases and sputum cytology was positive in 50 cases [23.4%]. Rigid bronchoscopy was made in 120 cases and this bronchoscopic cytology including bronchial washing and bronchial biopsy was positive in 34 cases [28.5%]. Bronchofiberscopy was performed in 94 cases and was positive in 45 cases [47.5%]. Histopathologically, 41 cases [43.6%] were epidermoid cell carcinoma, 8 cases [8.5%]of undifferentiated cell type, 12 cases [12.8%]of adenocarcinoma, 8 cases [8.5%]of alveolar cell type, and the 25 cases were undetermined. Cytologic examination of the sputum lacks the accuracy of the bronchoscopies in terms of both localization and accurate histologic indentification of the type of neoplasm. Rigid bronchoscope has the advantage of permitting identification of a tumor in a central location and of providing a sufficient amount of biopsy material for accurate diagnosis of carcinoma. However, it has the disadvantage of limiting examination to the larger, more central portions of the tracheobronchial tree. Bronchofiberscope had the advantage of examine upper lobe as well as other portions of the tracheobronchial tree which could not be visualized with the rigid bronchoscopy. A positive diagnosis in bronchofiberscopy was obtained in the highest rate, 47. 8% [45 cases]. A1 last, if a bronchogenic carcinoma is suspected on the basis of either symptoms of an abnormality on the chest film the diagnostic work-up-sputum cytology, bronchial washing, bronchoscopic biopsy, scalene node biopsy, thoracentesis and mediastinoscopy explothoracotomy etc-should precede in an attempt not only to obtain the higher positive diagnosis but also to obtain a tissue diagnosis and to evaluate the stage of the disease and to ascertain the appropriate mode of therapy.

• Journal of the Korean Society for Nondestructive Testing
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• v.32 no.1
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• pp.47-55
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• 2012

A structural health monitoring technique for locating impact position in a plate structure is presented in this paper. The method employs a single sensor and spatial focusing of time reversal (TR) acoustics. We first examine the TR focusing effect at the impact position and its surroundings through simulation and experiment. The imaging results of impact points show that the impact source location can be accurately estimated in any position of the plate. Compared to existing techniques for locating impact or acoustic emission source, the proposed method has the benefits of using a single sensor and not requiring material properties and geometry of structures. Furthermore, it does not depend on a particular mode of dispersive Lamb waves that is frequently used in other ultrasonic testings of plate-like structures.

• Journal of Ocean Engineering and Technology
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• v.19 no.6 s.67
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• pp.78-85
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• 2005

Initial alignment and localization are important topics in inertial navigation systems, since misalignment and initial position error wholly propagate into the navigation systems and deteriorate the performance of the systems. This paper presents the error convergence characteristics of the hybrid navigation system for underwater vehicles initial position, which is based on an inertial measurement unit (IMU) accompanying a range sensor. This paper demonstrates the improvement on the navigational performance oj the hybrid system with the range information, especially focused on the convergence of the estimation of underwater vehicles initial position error. Simulations are performed with experimental data obtained from a rotating ann test with a fish model. The convergence speed and condition of the initial error removal for random initial position errors are examined with Monte Carlo simulation. In addition, numerical simulation is conducted with an AUV model in lawn-mowing survey mode to illustrate the error convergence of the hybrid navigation System for initial position error.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.10
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• pp.1327-1336
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• 2001

The structural modes driven by the low wave-number components of smooth elastic wall pressure provide a relatively weak coupling between the flow and the wall motion. If the elastic thin plate has any resonant mode whose wave-number of resonance coincides with $\omega$/U$\sub$c/, the power will be transmitted to those modes of vibration by the flows. We examine the problem in which the elastic thin plate is subject to pressure fluctuations under turbulent boundary layer. Measurements are presented of the frequency spectra of the near- and far-field pressures and radiated sound contributed by the various wave modes of the thin elastic plate. Dispersion equation for wave motions of elastic plate is used to investigate the effect of bending waves of relatively low wave number on radiated sound. The low wave-number motion of elastic plate is observed to have much less influence on the low-frequency energy of wall pressure fluctuations than that of the rediated sound. High amplitude events of the wall pressure are observed to weakly couple with high-frequency energy of radiated sound for case of low tension applied to the plate. The sound source localization is applied to the measurement of radiated sound by using acoustic mirror system.