• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.221-225
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• 2005

An in vitro study was conducted to examine the effect of monensin or fish oil addition on bio-hydrogenation of $C_{18^-} unsaturated fatty acids and CLA production by mixed ruminal bacteria when incubated with safflower oil. Commercially manufactured concentrate (1%, w/v) with safflower oil (0.2%, w/v) were added to mixed solution (600 ml) of strained rumen fluid and McDougalls artificial saliva (control). Monensin $Rumensin^{(R)}$, 10 ppm, w/v, MO), mixed fish oil (0.02%, w/v, absorbed to 0.2 g alfalfa hay, FO) or similar amounts of monensin and fish oil (MO+FO) to MO and FO was also added into the control solution. All the culture solutions prepared were incubated in the culture jar anaerobically at $39^{\circ}C$ up to 12 h. Higher pH (p<0.047) and ammonia concentration (p<0.042) were observed from the culture solution containing MO at 12 h incubation than those from the culture solutions of control or FO. The MO supplementation increased (p<0.0001-0.007) propionate proportion of culture solution but reduced butyrate proportion at 6 h (p<0.018) and 12 h (p<0.001) of incubations. Supplementation of MO or MO+FO increased (p<0.001) the proportions of $C_{18:2}$. The MO alone reduced (p<0.022-0.025) the proportion of c9,t11-CLA compared to FO in all incubation times. The FO supplementation increased the proportion of c9,t11-CLA. An additive effect of MO to FO in the production of c9,t11-CLA was observed at 6 h incubation. In vitro supplementation of monensin reduced hydrogenation of $C_{18^-}$UFAs while fish oil supplementation increased the production of CLA.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1743-1748
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• 2003

The effect of pH on the fermentation characteristics and the formation of cis-9, trans-11 conjugated linoleic acid (CLA) and trans-11 octadecenoic acid by mixed ruminal bacteria was examined in vitro when incubated with linseed or rapeseed. Concentrate (1%, w/v) with ground linseed (0.6%, w/v) or rapeseed (0.5%, w/v) was added to 600 ml mixed solution of strained rumen fluid with artificial saliva (1:1, v/v), and was incubated anaerobically for 12 h at $39^{\circ}C$. The pH of culture solution was maintained at level close to 4.5, 5.3, 6.1 and 6.9 with 30% $H_2SO_4$ or 30% NaOH solution. pH increment resulted in increases of ammonia and total volatile fatty acid (VFA) concentration in culture solutions containing both oilseeds. Fermentation did not proceeded at pH 4.5. Molar proportion of acetate decreased but that of propionate increased as pH increased when incubated with oilseeds. While the hydrogenating process was very slow at the pH range of 4.5 to 5.3, rapid hydrogenation was found from the culture solutions of pH 6.1 and 6.9 when incubated with linseed or rapeseed. As pH in culture solution of linseed or rapeseed increases proportions of oleic acid (cis-9 $C_{18:1}$) and trans-11 octadecenoic acid increased but those of linoleic acid and linolenic acid decreased. The CLA proportion increased with pH in culture solution containing rapeseed but CLA was mostly not detected from the incubation of linseed.

• 패션비즈니스
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• 제19권4호
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• pp.57-74
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• 2015

The history of foot-binding was once regarded as a unique practice accentuating the beauty of a woman and the culture of the costume, to be found nowhere except in China. This thesis makes it an aim to understand the culture of costume in China by analyzing the beauty of foot-binding, and its meanings associated with the historical background of the costume. The method of investigation was by both previous literature on the topic and case analysis. The shoes were analyzed by colors, patterns and decorations; selecting 70 pieces easily accessible in the Tianjin Huaxia Shoes Culture Museum. In terms of form, the general type was of the most frequent, followed by one with an elevated toe, mixed type, and one of the downward toe, sequentially. The color of foot-binding shoes were mostly vivid chromatic colors, while the achromatic ones like black and grey constituting significant portions. In view of the patterns, plants patterns were the most frequent, followed by the mixed patterns of mostly flowers with butterflies, bats, and birds desiring the prosperity of descendants. In view of the ornaments, embroidery was the most frequent, while the mixed shoes included tassels, sequins, applique, and shoes without ornaments were of distributions. The forms, colors, patterns and ornaments clearly associated with the wishes of artistic beauty, sexuality, and rise in women's social status may be in the hope of leading others to understand the costume culture embedded in the Chinese by providing important clues about them.

• 한국식품과학회지
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• 제11권3호
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• pp.200-205
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• 1979

인산 염(monosodium phosphate, disodium phosphate, trisodium phosphate, ${\alpha}-polygel$ 및 sodium tripolyphosphate)이 Lactobacillus bulgaricus 단독 배양 및 Lactobacillus bulgari쳐s와 Streptococcus thermophilus의 혼합 배양(혼합비 1:1)에 미치는 영향을 검토하였다. 인산 염은 유산균의 생장을 촉진하였으며 특히 단독 배양의 경우 MSP와 STPP가, 혼합 배양의 경우 MSP와 DSP가 가장 효과적이었다. 산도도 유산균의 증식과 동일한 결과를 보였다. 배양액을 실온에서 저장하는 동안 생균 수의 감소는 인산 염 처리구가 대조구에 비하여 완만하였으며 단독 배양시 특히 DSP와 TSP가 효과적이었고 혼합 배양의 경우 TSP와 ${\alpha}-PG$가 가장 효과적이었다. 혼합 균주를 사용하여 제조된 발효유의 저장 중 안정성은 인산염 처리구가 대조구보다 좋았으며 특히 MSP가 가장 효과적이었다.

• International Journal of Advanced Culture Technology
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• 제8권1호
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• pp.182-187
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• 2020

Silver nanoparticles (AgNPs) have been manufactured in recent years and widely used in various fields. Reactive oxygen species (ROS), which occur in AgNPs, destroy cell membranes. It is widely accepted that ROS generated in this manner inhibit microorganisms growth and causes toxic effects, However, it does not affect cell membranes directly but positively affects growth in plants with cell walls. The nanoball used in this experiment is a new material that generates ROS stably and is used in aqueous solution. Results of this study indicate a 30% increase in yield of Ginseng mixed with culture soil. The analysis of soil condition after cultivation showed that the possibility of repetitive cultivation in soil mixed with Nanoball was high. This suggests that Nanoball is an antimicrobial active material due to the microbial / extermination effect of pathogenic microorganisms. Therefore, there may be potential applications in agricultural cultivation sites as a repetitive cultivation technology that reuses soil.

• 한국식품영양학회지
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• 제1권2호
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• pp.56-63
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• 1988

Fermented barley food was produced by the mixed culture fermentation with a tai-cults re system of a mold, a yeast and a lactobacillus. When Rhizopus delemar IFO 4746, Hansenular anomala IFO 0568 and Lactobacillus sp. L-5 were selected and cultivated on steamed barley at 3$0^{\circ}C$ for 2days and 37$^{\circ}C$ for 3days, the fermented product of good quality was obtained. During fermentation. changes in acidity, pH, water content and color of fermented barley were examined.

• 미생물학회지
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• 제42권4호
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• pp.277-285
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• 2006

본 연구에서는 신경인성 방광으로 요도 카테터를 유치하고 있는 환자의 카테터로부터 카테터 내 요로감염(Catheter-Associate Urinary Tract Infection; CA-UTI)에 관여하는 박테리아인 Escherichia coli, Pseudomonas aeruginosa 그리고 Staphylococcus aureus를 순수 분리, 동정하였다. 이 균주들을 대상으로 하여 quorum sensing mechanism을 규명하는 기초 연구로 각 균주의 quorum sensing 신호물질인 autoinducer (AIs)를 합성하는 유전자의 mRNA 발현을 확인하고, 정략분석 하였다. 각 세 균주를 단일과 세 균주의 혼합으로 24시간, 30일 동안 배양하며 일정 시간 간격으로 sample을 얻었다. 이 중 24시간 배양한 sample을 가지고 reverse transcription polymerase chain reaction (RT-PCR)을 수행하여 각 AIs 합성 유전자가 발현되는 최초 박테리아 밀도를 확인하였다. 단일배양에서 E. coli와 S. aureus의 AIs 합성 유전자(ygaG와 luxS)의 mRNA가 발현되는 최초 박테리아 밀도는 $2.4{\times}20^5$ CFU/ml, $5.4{\times}10^6$ CFU/ml 이었으며 P. aeruginosa의 rhlI와 lasI의 경우 $6.9{\times}10^4$ CFU/ml로 나타났다. 세 균주의 혼합배양에서 ygaG와 luxS의 mRNA가 발현되는 최초 박테리아 밀도는$7.3{\times}10^5$ CFU/ml, $1.6{\times}10^7$ CFU/ml이었으며 rhlI와 lasI의 경우 $2.1{\times}10^5$ CFU/ml로 나타났다. 또한 30일 배양한 sample의 RT-PCR 결과, 배양초기부터 각 AIs 합성 유전자들의 mRNA가 30일 동안 일정한 양만큼 지속적으로 발현됨을 확인하였다. Real-time RT-PCR을 이용한 AIs 합성 유전자의 mRNA 발현을 정량 분석한 결과 각 균주에서 단일배양보다 혼합배양시 AIs 합성 유전자의 발현이 더 많았다. 가장 많은 발현량의 차이를 보인 경우 E. coli ygaG의 mRNA 발현량은 단일배양보다 세 균주의 혼합배양시 최고 약 30배 이상이 증가하였고, P. aeruginosa rhlI의 경우 단일배양보다 혼합배양시 최고 약 40배, P. aeruginosa lasI의 경우 최고 약 250배 그리고 S. aureus luxS의 경우는 단일배양보다 혼합배양시 최고 약 5배 이상 mRNA 발현량이 증가하였다. 또한 세 균주의 4가지 유전자 중 P. aeruginosa의 rhlI와 lasI의 mRNA가 가장 많은 양으로 발현됨을 확인하였다.

• 패션비즈니스
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• 제9권6호
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• pp.71-85
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• 2005

The purpose of this study is to prove that interchange is the a key factor in costume style changes through case analysis in costume culture interchange, and further, to assist in gaining understanding of costume style changes of the present and the future. Study findings show that the pattern of acceptance of costume interchange was mostly integration, though it was influenced by the method of transmission. The integration is a process in which a foreign costume culture is constructively mixed into the existing one, and a factor that changes the existing costume culture.

• 한국식생활문화학회지
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• 제21권6호
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• pp.661-669
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• 2006

The purpose of this study was to investigate the consumption and perception on the effect of cooked rice mixed with multi-grain (CR-MG). Reason for like and dislike, intake frequency and variables related with the satisfaction of CR-MG were also investigated. The data for the analysis was collected from 315 adults who were residing at Seoul${\cdot}$Kyeonggi and Kangwon area and analyzed by the SPSS 11.0 program. The result showed that 84.8% of the respondents consumed CR-MG, showing significant difference by gender and occupation (p<0.05). It also revealed that they consumed it knowing their effect, showing significant difference by all general characteristics except for resident area and BMI, respectively. Kind of multi-grain (MG) mixed with cooked rice was high in the order of SoRiTae (80.1%) > brown rice (73.4%) > black rice (72.7%) > waxy rice (61.0%), respectively. As for the mixing type of MG, 61.4% of the respondents preferred non-mixed product rather than pre-mixed product. 2 or 3 kinds were preferred numbers of MG mixed with rice, showing significant difference by age (p<0.001) and marital status(p<0.05), respectively. 59.9% of the respondents answered 'high nutritive value more than polished rice' as the major reason for like, whereas 31.3% answered 'greatly rough more than polished rice' as that for dislike, respectively. By the use of multiple regression analysis, it revealed that health was the most significant variable which affect the satisfaction of CR-MG. From these results, it was suggested that in view of the high consumption and intention to continue the intake of CR-MG, following studies should be conducted to improve the preference of CR-MG.

• 대한환경공학회지
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• 제22권7호
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• pp.1243-1252
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• 2000

휘발성 훈증제인 cis-와 trans-1,3-D의 각각에 대한 분해력 향상은 몇몇 연구자들에 의해 이루어져왔다. 본 연구는 cis-와 trans-1,3-D에 대해 서로 다른 속도에서 각각의 분해력 증진과 미생물과의 관련성을 조사한 것으로, 미생물이 휘발성을 갖는 독성 유기화합물의 분해를 향상시키는 데 관여하고 있음이 관찰되었다. 1,3-D로 야외(field)처리되어 적응되어 왔던 토양으로부터, 1,3-D의 분해가 확인된 토양시료를 채취하여 1,3-D를 분해할 수 있는 혼합 배양세균을 분리하였다. 이렇게 분리된 혼합 배양세균은 cis-1,3-D보다는 trans-1,3-D를 더 빨리 분해 시켰으나, 미생물 성장을 위한 탄소원으로 cis-와 trans-1,3-D만이 제공되었을 때는 분해가 일어나지 않는 반면, 적절한 2차 탄소원들이 존재할 때에는 cis-와 trans-1,3-D를 분해시켰다. 따라서, cis-와 trans-1,3-D의 분해는 공동대사과정(cometabolism)인 것으로 판단된다. 두 이성질체는, 토양여과액(soil leachate), tryptone, tryptophan, alanine이 포함된 시료가 2차 탄소원으로 제공되었을 때에는 분해가 이루어졌으나, 고온고압하에서 멸균시킨 토양추출액(soil extract), glucose, yeast extract 및 indole이 포함된 시료가 2차 탄소원으로 제공되었을 때는 두 이성질체 모두를 분해시키지 못했다. 상업용 훈증제로 이용되는 cis-와 trans-1,3-D를 다른 속도로 개별적으로 분해하는 혼합 미생물군은 형태학적인 구별방법에 의해 4개의 독립된 순수 colony로 구성되어 있는 것으로 관찰되었다.