• 자연과학논문집
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• 제1권
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• pp.115-198
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• 1987

The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

• 유기물자원화
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• 제27권2호
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• pp.19-30
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• 2019

본 연구는 국내산 우각이 혼합된 유기질비료를 개발하여 가장 많은 유기재배 면적을 차지하고 있는 벼를 포함하여 가지에 대한 시용효과를 조사하고 수입 유박을 대체할 유기자원으로 우각의 활용가능성을 구명하고자 실시하였다. 질소함량이 높은 국내산 유기자원 선발을 위하여 계분, 어분, 콩깻묵, 참깻묵, 들깻묵, 혈분, 우각, 맥주오니 등 8종을 분석하여 질소함량이 높은 유기자원을 선발하였고 보조제로 왕겨 바이오차, 미강 등을 원료별, 혼합비율별로 혼합하고 성분을 분석하여 유기농업에 사용 가능한 유기질비료 제조조건을 확립하였다. 우각은 전질소(T-N) 함량이 12.0 %로 높아 혈분 13.5 % 다음으로 높았으며 어분 및 깻묵은 전질소 함량이 5.9~7.9 % 수준이었다. 계분은 유기농업에 사용가능한 무항생제 산란계 계분을 사용하였으며, 맥주오니는 질소함량이 3.4 %로 나타났다. 무항생제 계분, 우각, 맥주오니 등을 주재료로 바이오차, 미강 등을 보조제로 사용하여 유기질비료를 제조한 결과. 수입유박의 질소함량(4.0~4.2 %) 대비 개발한 유기질비료의 질소함량은 7.5 %로 높고 중금속함량은 Zn 400 mg/kg, Cu 120 mg/kg 이하 등으로 나타나 질소 함량이 높고 유기농업자재 품질기준에 적합한 유기질비료를 개발하였다. 우각이 포함된 유기질비료를 사용하여 벼와 가지를 재배하면서 시용효과를 조사한 결과 토양검정질소시비량 기준 100 % 시용시 혼합유박 대비 시용량을 40 % 감소하였음에도 벼 생육 및 수량이 대등하였으며, 가지 재배시에도 동일한 결과를 나타내었다. 우각 등 국산 유기자원을 이용한 새로운 고농도 질소원 선발 및 이를 이용한 유기재배 적합 유기질비료 개발은 친환경농업 확대 보급의 중요성이 높아지고 있는 현 시점에서 지역자원을 이용한 기존 수입 혼합유박 대체 연구의 출발점이자 폐기되고 있는 국내 유기자원의 활용 방안 모색에서 큰 의미를 가지며 향후 확대 보급된다면 친환경농산물의 안정적 생산에 기여할 수 있을 것으로 기대된다.

• 헤리티지:역사와 과학
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• 제46권4호
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• pp.108-125
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• 2013

경주 동천동 출토 토제범(土製范)에 대한 실측복원도, 조성광물 분석, 입도 및 물성시험, 미세조직관찰, 색도차 및 주성분 분석결과를 알아보았다. 연구대상 내범과 외범의 단면은 내측(제1층위)과 외측(제2층위)으로 구분되고 외측에는 유기물이 혼합되어 있다. 단면에는 열적 경험과 탈형제로 인한 변화 양상이 확인되며, X-선 촬영에서는 내측인 제1층위가 투과율이 높은 광물로 구성되었고 편광현미경에서 석영만이 관찰되었다. SEM 관찰에서 단면의 내측은 공극이 확대된 양상을 나타내고 중앙은 균열이 발달하였으며 외측에서는 변화를 관찰할 수 없었다. 구성입자의 입도는 내범과 외범이 거의 유사하며 미사질식양토로 구분되고, 미사질과 점토의 구성비는 약 2.7 : 1과 약 2.9 : 1이었다. 물성시험에서 내범과 외범의 밀도와 흡수율은 유사하고 공극률만이 내범이 27.36%와 외범은 31.09%로 다소 차이가 있었다. 단면의 색도차는 제1층위의 표면에 탈형을 위해 그을음을 입히거나 제1층위를 먹물로 수비하였을 가능성이 있다. 조성광물 분석에서는 내범과 외범의 조성광물이 동일하나 내범에서만 자철석이 동정되었다. 주성분 분석에서는 $SiO_2$의 평균함량이 71.64%, $Al_2O_3$는 14.59%이고, 부성분 중 $Fe_2O_3$는 4.51%, $K_2O$는 3.06%이며, $Na_2O$, MgO, CaO, $TiO_2$, $P_2O_5$, MnO는 2% 이하를 나타내었다.

• 기록학연구
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• 제74호
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• pp.45-78
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• 2022

남아프리카공화국은 인종분리정책인 아파르트헤이트를 1948년부터 1994년까지 시행했다. 이 정책의 주요 내용은 백인과 인도인, 혼혈인, 흑인 등 인종을 계급화하고, 계층에 따라 거주지와 개인 재산 소유, 경제적 활동 등 모든 사회적 행위를 제한하는 것이었다. 백인을 제외한 모든 인종이 피부색이 다르다는 이유로 차별받았고, 탄압받았다. 남아프리카공화국 시민들은 무차별한 폭력을 일삼는 정부에 저항하였고, 이들을 지탄하는 여론은 지역 사회를 넘어 세계 곳곳으로 확대되었다. 이를 가능하게 한 것 중 하나는 폭력 현장을 자세히 기록한 사진 이미지들이다. 민중 탄압을 포착했던 외신 기자들은 물론 남아프리카공화국 출신의 사진작가들은 개인의 차원에서 소외되고 고통받는 사람들의 삶을 기록하는데 몰두했다. 이들이 현실을 알리려는 의지가 없었고 실제로 이를 사진으로 기록하지 않았다면, 많은 사람이 인종 차별로 기인한 사태의 참혹함을 알지 못했을 것이다. 이에 본 논문은 아파르트헤이트의 여러 단면을 포착해 그와 관련한 여러 기록을 전시한 『Rise and Fall of Apartheid: Photography and the Bureaucracy of Everyday Life』를 주목하고, 사진에서 묘사된 남아프리카공화국에서 자행된 인종 차별의 면면을 살펴보고자 한다. 이 전시는 아파르트헤이트가 시작된 1948년부터 넬슨 만델라가 대통령으로 선출된 후, 잘못된 역사관을 바로잡으려는 진실화해위원회가 출범한 1995년까지의 시기를 다룬다. 전시에 출품된 수많은 사진은 대중에게도 잘 알려진 피터 마구베네(Peter Magubane), 이안 베리(Ian Berry), 데이비드 골드블라트(David Goldblatt), 산투 모포켕(Santu Mofokeng) 등이 촬영한 것으로, 다양한 아카이브를 비롯한 박물관, 미술관, 언론 매체의 소장품이다. 전시에 출품된 사진들은 일차적으로는 사진작가들의 작업 결과물이다. 사진 작품인 동시에 1960년대 이후 남아프리카공화국의 과거를 증명하는 매체이지만 역사학이나 기록학적인 관점보다는 사진사와 미술사의 영역에서 주로 다루어졌다. 하지만 전시된 사진들은 기록물로서의 특성이 있고, 그 안에 담긴 맥락 정보는 역사를 다양한 시각으로 되짚어볼 수 있는 특징이 있다. 따라서 기존에 연구된 영역에서 확대하여 다양한 시선으로 당대를 살펴보고, 이를 새롭게 해석하는 것이 매우 중요하다. 전시에서 선보인 사진 작품들은 남아프리카공화국의 공식 기록에 포함되지 않은 사건과 사람을 증명하고 서술한다. 이런 점은 보통 사람들의 기억, 개인의 기록을 통해 사회적으로 소외된 인물과 사건을 역사적 공백으로 편입시키고, 여러 매체로 재생산되어 기록 생산의 맥락을 강화하고 확산시킨다는 점에서 그 의의를 찾을 수 있다.

• 산업경영시스템학회지
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• 제46권1호
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• pp.91-104
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• 2023

The records system is believed to have started in Italy in the 14th century in line with trade developments in Europe. In 1491, Luca Pacioli, a mathematician, and an Italian Franciscan monk wrote the first book that described double-entry accounting processes. In many countries, including Korea, the government accounting standards used single-entry bookkeeping rather than double-entry bookkeeping that can be aggregated by account subject. The cash-based and single-entry bookkeeping used by the government in the past had limitations in providing clear information on financial status and establishing a performance-oriented financial management system. Accordingly, the National Accounting Act (promulgated in October 2007) stipulated the introduction of double-entry bookkeeping and accrual accounting systems in the government sector from January 1, 2009. Furthermore, the Korean government has also introduced International Financial Reporting Standards (IFRS), and the System of National Accounts (SNA). Since 2014, Korea owned five national accounts. In Korea, valuation began with the 1968 National Wealth Statistics Survey. The academic origins of the valuation of national wealth statistics which had been investigated by due diligence every 10 years since 1968 are based on the 'Engineering Valuation' of professor Marston in the Department of Industrial Engineering at Iowa State University in the 1930s. This field has spread to economics, etc. In economics, it became the basis of capital stock estimation for positive economics such as econometrics. The valuation by the National Wealth Statistics Survey contributed greatly to converting the book value of accounting data into vintage data. And in 2000 National Statistical Office collected actual disposal data for the 1-digit asset class and obtained the ASL(average service life) by Iowa curve. Then, with the data on fixed capital formation centered on the National B/S Team of the Bank of Korea, the national wealth statistics were prepared by the Permanent Inventory Method(PIM). The asset classification was also classified into 59 types, including 2 types of residential buildings, 4 types of non-residential buildings, 14 types of structures, 9 types of transportation equipment, 28 types of machinery, and 2 types of intangible fixed assets. Tables of useful lives of tangible fixed assets published by the Korea Appraisal Board in 1999 and 2013 were made by the Iowa curve method. In Korea, the Iowa curve method has been adopted as a method of ASL estimation. There are three types of the Iowa curve method. The retirement rate method of the three types is the best because it is based on the collection and compilation of the data of all properties in service during a period of recent years, both properties retired and that are still in service. We hope the retirement rate method instead of the individual unit method is used in the estimation of ASL. Recently Korean government's accounting system has been developed. When revenue expenditure and capital expenditure were mixed in the past single-entry bookkeeping we would like to suggest that BOK and National Statistical Office have accumulated knowledge of a rational difference between revenue expenditure and capital expenditure. In particular, it is important when it is estimated capital stock by PIM. Korea also needs an empirical study on economic depreciation like Hulten & Wykoff Catalog A of the US BEA.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.45-46
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• 2015

Clubroot disease of crucifers has occurred since 1957. It has spread to the whole China, especially in the southwest and nourtheast where it causes 30-80% loss in some fields. The disease has being expanded in the recent years as seeds are imported and the floating seedling system practices. For its effective control, the Ministry of Agriculture of China set up a program in 2010 and a research team led by Dr. Yueqiu HE, Yunnan Agricultural University. The team includes 20 main reseachers of 11 universities and 5 institutions. After 5 years, the team has made a lot of progresses in disease occurrence regulation, resources collection, resistance identification and breeding, biological agent exploration, formulation, chemicals evaluation, and control strategy. About 1200 collections of local and commercial crucifers were identified in the field and by artificiall inoculation in the laboratories, 10 resistant cultivars were breeded including 7 Chinese cabbages and 3 cabbages. More than 800 antagostic strains were isolated including bacteria, stretomyces and fungi. Around 100 chemicals were evaluated in the field and greenhouse based on its control effect, among them, 6 showed high control effect, especially fluazinam and cyazofamid could control about 80% the disease. However, fluzinam has negative effect on soil microbes. Clubroot disease could not be controlled by bioagents and chemicals once when the pathogen Plasmodiophora brassicae infected its hosts and set up the parasitic relationship. We found the earlier the pathogent infected its host, the severer the disease was. Therefore, early control was the most effective. For Chinese cabbage, all controlling measures should be taken in the early 30 days because the new infection could not cause severe symptom after 30 days of seeding. For example, a biocontrol agent, Bacillus subtilis Strain XF-1 could control the disease 70%-85% averagely when it mixed with seedling substrate and was drenching 3 times after transplanting, i.e. immediately, 7 days, 14 days. XF-1 has been deeply researched in control mechanisms, its genome, and development and application of biocontrol formulate. It could produce antagonistic protein, enzyme, antibiotics and IAA, which promoted rhizogenesis and growth. Its The genome was sequenced by Illumina/Solexa Genome Analyzer to assembled into 20 scaffolds then the gaps between scaffolds were filled by long fragment PCR amplification to obtain complet genmone with 4,061,186 bp in size. The whole genome was found to have 43.8% GC, 108 tandem repeats with an average of 2.65 copies and 84 transposons. The CDSs were predicted as 3,853 in which 112 CDSs were predicted to secondary metabolite biosynthesis, transport and catabolism. Among those, five NRPS/PKS giant gene clusters being responsible for the biosynthesis of polyketide (pksABCDEFHJLMNRS in size 72.9 kb), surfactin(srfABCD, 26.148 kb, bacilysin(bacABCDE 5.903 kb), bacillibactin(dhbABCEF, 11.774 kb) and fengycin(ppsABCDE, 37.799 kb) have high homolgous to fuction confirmed biosynthesis gene in other strain. Moreover, there are many of key regulatory genes for secondary metabolites from XF-1, such as comABPQKX Z, degQ, sfp, yczE, degU, ycxABCD and ywfG. were also predicted. Therefore, XF-1 has potential of biosynthesis for secondary metabolites surfactin, fengycin, bacillibactin, bacilysin and Bacillaene. Thirty two compounds were detected from cell extracts of XF-1 by MALDI-TOF-MS, including one Macrolactin (m/z 441.06), two fusaricidin (m/z 850.493 and 968.515), one circulocin (m/z 852.509), nine surfactin (m/z 1044.656~1102.652), five iturin (m/z 1096.631~1150.57) and forty fengycin (m/z 1449.79~1543.805). The top three compositions types (contening 56.67% of total extract) are surfactin, iturin and fengycin, in which the most abundant is the surfactin type composition 30.37% of total extract and in second place is the fengycin with 23.28% content with rich diversity of chemical structure, and the smallest one is the iturin with 3.02% content. Moreover, the same main compositions were detected in Bacillus sp.355 which is also a good effects biocontol bacterial for controlling the clubroot of crucifer. Wherefore those compounds surfactin, iturin and fengycin maybe the main active compositions of XF-1 against P. brassicae. Twenty one fengycin type compounds were evaluate by LC-ESI-MS/MS with antifungal activities, including fengycin A $C_{16{\sim}C19}$, fengycin B $C_{14{\sim}C17}$, fengycin C $C_{15{\sim}C18}$, fengycin D $C_{15{\sim}C18}$ and fengycin S $C_{15{\sim}C18}$. Furthermore, one novel compound was identified as Dehydroxyfengycin $C_{17}$ according its MS, 1D and 2D NMR spectral data, which molecular weight is 1488.8480 Da and formula $C_{75}H_{116}N_{12}O_{19}$. The fengycin type compounds (FTCPs $250{\mu}g/mL$) were used to treat the resting spores of P. brassicae ($10^7/mL$) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A260) and at 280 nm (A280) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be lyzed by the FTCPs of XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol. In the five selected medium MOLP, PSA, LB, Landy and LD, the most suitable for growth of strain medium is MOLP, and the least for strains longevity is the Landy sucrose medium. However, the lipopeptide highest yield is in Landy sucrose medium. The lipopeptides in five medium were analyzed with HPLC, and the results showed that lipopeptides component were same, while their contents from B. subtilis XF-1 fermented in five medium were different. We found that it is the lipopeptides content but ingredients of XF-1 could be impacted by medium and lacking of nutrition seems promoting lipopeptides secretion from XF-1. The volatile components with inhibition fungal Cylindrocarpon spp. activity which were collect in sealed vesel were detected with metheds of HS-SPME-GC-MS in eight biocontrol Bacillus species and four positive mutant strains of XF-1 mutagenized with chemical mutagens, respectively. They have same main volatile components including pyrazine, aldehydes, oxazolidinone and sulfide which are composed of 91.62% in XF-1, in which, the most abundant is the pyrazine type composition with 47.03%, and in second place is the aldehydes with 23.84%, and the third place is oxazolidinone with 15.68%, and the smallest ones is the sulfide with 5.07%.