• Child Health Nursing Research
• /
• 제6권2호
• /
• pp.158-166
• /
• 2000

The purpose of this study was to confirm the efficient umbilical cord care of healthy newborns in nursery. In order to determine the efficient care, the time of umbilical cord separation and the skin condition of periumbilical area were evaluated. The data were collected in sample of 529 normal, healthy newborns of C hospital in Seoul, from September 1st, 1999 to January 16th, 2000. The babies were randomly selected and allocated into four groups-alcohol swab/tub bath, alcohol swab/partial bath, natural dry/tub bath, natural dry/partial bath- by the methods of care. The mothers of babies were also surveyed by questionnaire about general characteristics. The data were analyzed by SAS program. The results of this study were as follows : 1. The average gestation period of newborns was 39+3 weeks. The average birth weight was 3.27Kg. In gender of babies, boys were 51.7% and girls were 48.3%. The 65% of newborns were born normal vaginal delivery, 35% were C-section. The feeding methods were 55.3% of the babies fed by mixed type, 22.9% by breast, and 21.8% by bottle. There was no significance among four groups by general characteristics. 2. The average time of umbilical cord separation was 8.27 days(SD=2.3). The time was no difference significantly among four groups(F=1.68, P=0.17). 3. The prevalence of the umbilical care complication did not show differences among four groups( 2=3.93, P=0.27). In conclusion, Nurses have preferred the traditional alcohol swab/partial bath method in care of newborns. But there was no difference among the ways to take care of umbilicus according to this study. The way to naturally is more efficient due to reduce the time and expense in umbilical care for normal babies of nursery. This also matched the change in approach to healthy population from intervention to nonintervention way. This could be suggested in education for normal and healthy babies care at home and nursery as a useful way. Endly, the further study about bacterial colony nda infection rate at umbilicus by ways of care is needed.

• 한국가금학회지
• /
• 제42권2호
• /
• pp.133-137
• /
• 2015

본 연구는 저 에너지 사료 내 lysolecithin과 sodium stearoyl-2-lactylate의 첨가가 육계의 생산성 및 영양소 소화율에 미치는 영향을 규명하였다. 본 시험은 1일령 ROSS 308(♂, ♀) 768수를 공시하였고, 시험 개시 체중은 44.3 g으로 35일간 진행하였으며, 시험설계는 1) PC(basal diet), 2) NC(PC-100 kcal), 3) T1(NC+ 0.08% lysolecithin) and 4) T2(NC + 0.04% sodium stearoyl-2-lactylate)로 4개 처리를 하여 처리당 12반복, 반복당 16수씩 완전 임의 배치하였다. 증체량(body weight gain: BWG), 사료섭취량(feed intake: FI) 및 사료요구율(feed conversion ratio: FCR)은 매주 측정하였다. 산화크롬(Cr2O3)을 표시물로서 0.2% 첨가하여 실험 실험종료 7일 전에 급여하였다. 1~21일차 생산성에 있어 처리구간 유의적인 차이가 나타나지 않았고(P>0.05), 21~35일차 증체량에 있어서 T1 처리구 및 T2 처리구가 대조구와 비교하였을 때 유의적으로 높게 나타났다(P<0.05). 영양소 소화율에 있어 T1 처리구 및 T2 처리구가 대조구와 비교하였을 때 유의적으로 효과가 있었다(P<0.05). 그러나 건물 소화율에 있어서는 처리구간 유의적인 차이를 나타내지 않았다(P>0.05). 종합적으로, 육계 사료 내 유화제의 첨가가 후반 성장 단계에서 생산성, 에너지 소화율 및 질소 소화율을 향상시켰다.

• KSBB Journal
• /
• 제15권2호
• /
• pp.174-180
• /
• 2000

본 연구에서는 다른 host cell 에 비하여 여러 가지 장점을 가지고 있는 Methylotrophic yeast 중 Pichia pastoris와 Hans-enula polymorpha의 fed batch 실험을 통하여 유전자 재조합 단백질 발현최적조건을 구하여 각 균주의 유전자 재조합 albumin 발현 최적화 연구를 수행하였다. 글리세롤이 두 균주의 promoter의 AOX 1과 MOX promoter repression에 미치는 영향을 확인한 바 H. polymorpha가 P. pastoris보다 promoter repression이 심함을 알 수 있었다. 두 균주의 promoter를 induction시키는 최적 메탄올 농도는 P. pastoris의 경우 메탄올 8g/L, H. polymorpha의 경우는 13 g/L임을 알 수 있었다. 또한 메탄올에 의한 induction시기는 두 균주 모두 O.D. 4 정도되는 exponential growth stage에서 메탄올을 첨가하는 경우가 초기 세포 성장단계에 메탄올을 첨가한 경우에 비해 약 20% 정도 높아짐을 확인하였다. 두 균주의 재조합 albumin 발현에 미치는 pH의 영향을 조사하였는데, p. pastoris의 경우 pH 5에서 가장 높은 albumin 생산성을 보여 약 300mg/L albumin을 발현하였고, H. polymorpha 의 경우 pH 5와 6에서 최대 약 180 mg/L의 albumin을 발현하였지만 pH 8에서는 이의 절반 수준에 그쳤다. 두 균주의 최적 fed-batch 방법을 확인하는 실험을 수행하였는데 P. pastoris의 경우의 최적 fed-batch 방법은 mixed feeding은 바람직하지 않고 글리세롤 배지를 공급하여 세포를 성장시킨 후 글리세롤 공급을 멈추고 바로 메탄올로 전환하는 방법을 효과적이며, H. polymorpha의 경우 비성장속도 제어를 통한 글리세롤 공급으로부터 메탄올 공급으로의 단계적 전환방법이 균주의 albumin 발현에 큰 영향을 준다는 것을 알 수 있었다. 이 방법을 통하여 두 균주의 고농도 배양 실험을 수행한 결과 P. pastoris의 경우는 O.D. 300에서 약 4.7g albumin/L를 발현하였다. 이와같은 결과를 바탕으로 산업체에서 methlotrophic yeast를 이용한 상업화를 계획함에 있어서 host로서의 균주를 선택할 수 있는 기본 자료를 제공함과 아울러 균주가 선택된 후에 그 균주를 이용한 재조합 단백질 최적화 방법을 제공하여 줄 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권11호
• /
• pp.1755-1762
• /
• 2020

Objective: Thyroid hormone responsive spot 14 alpha (THRSP) has been used to investigate the regulation of de novo lipogenesis because the variation of THRSP mRNA content in the tissue affects directly the ability of that tissue to synthetize lipids. Also, this gene responds to thyroid hormone stimulation and high level of carbohydrate feeding or insulin-injection. This study was carried out to investigate variations within THRSP and their effects on body and carcass weights in Korean native chicken (KNC). Methods: A total of 585 chickens which represent the five lines of KNC (Black, Gray-Brown, Red-Brown, White, and Yellow-Brown) were reared and body weight data were recorded every two weeks from hatch until 20 weeks of age. Polymerase chain reaction- restriction fragment length polymorphism, DNA chips for Agilent 2100 Bioanalyzer, and Fluidigm Genotyping Technology, were applied to genotype selected markers. A linear mixed-effect model was used to access association between these single nucleotide polymorphism (SNP) markers and growth-related traits. Results: A total of 30 polymorphisms were investigated in THRSP. Of these, nine SNPs for loci were selected to perform association analyses. Significant associations were detected between g.-49G>T SNP with body weight at 20 weeks of age (BW20), g.451T>C SNP with growth at 10 to 12 weeks of age (GR10-12), and g.1432A>C SNP with growth at 14 to 16 weeks trait (GR14-16) and body weight at 18 weeks of age (BW18). Moreover, diplotype of the THRSP gene significantly affected body weight at 12 weeks of age (BW12) and GR10-12 traits. Diplotype of ht1/ht2 was favorable for BW12 and GR10-12 traits. Conclusion: These results suggest that THRSP can be regarded as a candidate gene for growth traits in KNC.

• 유기물자원화
• /
• 제14권4호
• /
• pp.104-112
• /
• 2006

지렁이 사육상을 조성하는데 있어서 적정한 초기 지렁이 입식 밀도를 추정하기 위해서 단위 면적당 서로 다른 밀도의 지렁이($1m^2$의 사육상 면적당 지렁이 1, 2, 3, 5kg)를 입식하여 12kg의 먹이를 반복 급이하면서 그의 개체군 밀도 변화를 조사하였고, 효율적인 먹이급이량을 추정하기 위하여 최고 한계에 근접한 지렁이 밀도($5kg/m^2$)에서 먹이급이량을 달리하였을 때($1m^2$의 사육상 면적당 먹이 16, 24, 32, 40kg) 먹이섭식속도를 조사하였다. 지렁이 구입비용, 노동비용 등을 고려하였을 때, 지렁이 사육상을 조성하기 위한 초기 입식밀도는 사육상 면적 $1m^2$당 2kg 이하로 하는 것이 합리적인 것으로 판단된다. 지렁이 사육상에 지렁이 밀도가 최고밀도에 근접한 이후에는 단위 사육 면적당 1회에 투입되는 먹이량은 $40kg/m^2$정도인 것이 그 보다 적은 경우보다 지렁이 증식과 먹이급이를 위한 작업효율 측면에서 유리하였다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권4호
• /
• pp.475-478
• /
• 2005

This study was conducted to evaluate feeding value of berseem and lucerne silage as a replacement for conventional fodder (berseem fodder) in lactating Nili buffaloes. Fifteen early lactating multi-parous Nili buffaloes, five buffaloes in each group were allotted three experimental diets. Berseem and lucerne fodders were ensiled at 30% DM (wheat straw was used to adjust the DM of fodders) with molasses (at the rate of 2% of fodder DM) in two bunker silos for 30 days. The diets contained 75% DM from berseem fodder (BF), 75% DM from berseem silage (BS) and 75% DM from lucerne silage (LS). Each diet contained 25% concentrate DM. Diets were mixed daily and fed twice a day at ad libitum intakes. Dry matter intake (DMI) was significantly higher (13.8 kg/d) in buffaloes fed BF diet than those fed LS (12.5 kg/d) and BS (11.9 kg/day) diets. The differences in digestible DMI and DMI as percent body weight were significant between fodder and silage based diets but non-significant when BS and LS were compared. Lower DMI with silage-based diets was probably because of low silage pH. Intake of NDF (NDFI) was higher (5.68 kg/d) in buffaloes fed BF diet followed by those fed LS (5.50 kg/d) and BS (5.00 kg/d) diets. The difference was significant (p<0.05) across fodder and silage based diets but NDFI was non-significant across both silage-based diets. The apparent DM digestibility was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Four percent fat corrected milk yield was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Higher milk yield with fodder based diet was because of more digestible nutrient intake (Table 3) compared with silage based diets. Milk CP, TP and NPN and SNF did not show any treatment effects. The present results indicated that the berseem and lucerne fodder ensiled at 30% DM level with 2% molasses could safely replace (75% DM) the conventional leguminous fodder in the diets of lactating Nili buffaloes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권4호
• /
• pp.493-499
• /
• 2011

The objective of this study was to find the way to prolong the storage time of sawdust-based oyster mushroom (Pleurotus osteratus) spent substrate (OMSS) by fermenting with potential probiotic microorganisms to recycle the otherwise waste of mushroom farms. To this purpose, lactic acid bacteria (LAB) were screened to select the best lactic acid-producing strains. Three strains of LAB (Lactobacillus plantarum Lp1', Pediococcus acidilacticii Pa193, L. plantarum Lp2M) were selected and in mixture they lowered the pH of the fermented OMSS to 3.81. fOMSS (fermented sawdust-based oyster mushroom spent substrate) could be stored at room temperature for at least 17 days without any deterioration of feed quality based on the pH, smell, and color. In dry matter disappearance rate in situ, commercial TMR (total mixed ration), OMSS and OMMM (oyster mushroom mycelium mass) showed no significant differences between the samples after 6, 12 and 24 h incubation except for 48 h. Two separate field studies were performed to test the effects of fOMSS supplement on the growth performance of postweaning Holstein calves. Field trials included groups of animals feeding calf starter supplemented with: Control (no supplement), AB (colistin 0.08% and oxyneo 110/110 0.1%), fOMSS (10% fOMSS) and fConc (10% fermented concentrate) and DFM (direct-fed microbials, average $10^9$ cfu for each of three LAB/d/head). Growth performance (average daily gain and feed efficiency) of the fOMSS supplement group was higher than that of AB followed by fConc and DFM even though there was no statistically significant difference. The Control group was lower than any other group. Various hematological values including IgG, IgA, RBC (red blood cell), hemoglobin, and hematocrit were measured every 10 days to check any unusual abnormality for all groups in trial I and II, and they were within a normal and safe range. Our results suggest that sawdust-based OMSS could be recycled after fermentation with three probiotic LAB strains as a feed supplement for post-weaning calves, and fOMSS has the beneficial effects of an alternative to antibiotics for a growth enhancer in dairy calves.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.40-46
• /
• 2018

Objective: To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture and an in vivo dairy cow experiment were conducted. Methods: The characteristics of in vitro ruminal fermentation were investigated using rumen fluids from six cannulated Holstein dairy cows at 2 h post-afternoon feeding. A control treatment was included in the experiments based on a typified total mixed ration (TMR) for lactating dairy cows, which was identical to the one used in the in vivo study, plus R. sphaeroides at 0.1%, 0.3%, and 0.5% TMR dry matter. The in vivo study employed six ruminally cannulated lactating Holstein cows randomly allotted to either the control TMR (C-TMR) treatment or to a diet supplemented with a 0.5% R. sphaeroides culture (S-TMR, dry matter basis) ad libitum. The presence of R. sphaeroides was verified using denaturing gradient gel electrophoresis (DGGE) applied to the bacterial samples obtained from the in vivo study. The concentration of CoQ10 in milk and in the supernatant from the in vitro study was determined using high performance liquid chromatography. Results: The results of the in vitro batch culture and DGGE showed that the concentration of CoQ10 significantly increased after 2 h of R. sphaeroides supplementation above 0.1%. When supplemented to the diet of lactating cows at the level of 0.5%, R. sphaeroides did not present any adverse effect on dry matter intake and milk yield. However, the concentration of CoQ10 in milk dramatically increased, with treated cows producing 70.9% more CoQ10 than control cows. Conclusion: The CoQ10 concentration in milk increased via the use of a novel DFM, and R. sphaeroides might be used for producing value-added milk and dairy products in the future.

• 한국식품영양과학회지
• /
• 제36권6호
• /
• pp.745-752
• /
• 2007

본 연구는 닭고기의 감마지방산 축적에 관한 서로 다른 수준의 달맞이꽃 종자유 급여효과를 조사하였다. 로스(Ross)종 1일령 broiler 수컷 600수를 6처리구${\times}$4반복으로 완전임의 배치하였다. 브로일러는 포화지방산 급원으로서 우지를 함유하는 대조구, 감마지방산 급원으로서 달맞이꽃 종자유(EPO, evening primrose oil) 0.5%, EPO 혼합유(EPO 70:대두유 30) 0.7%, EPO 1.5%, EPO 3.0% 및 EPO 4.0% 첨가사료를 브로일러 후기 2주 동안 섭취하였다. 체중은 0.5% EPO 첨가구를 제외하고는 대조구와 처리구간 통계적유의차가 있었다(p<0.05). 도체중에 대한 다리살과 가슴살의 무게비율은 다리살에서 0.5% EPO 첨가구 및 가슴살에서 0.5% EPO 첨가구와 4.0% EPO 첨가구를 제외하고는 대조구와 처리구간 유의성이 인정되었다(p<0.05). EPO 첨가사료를 섭취한 닭고기 피부와 가슴살 지질의 포화지방산 조성은 대조구에 비해서 유의적으로 낮아졌으나 불포화지방산 조성은 대조구와 비교할 때 EPO 첨가구가 높았다(p<0.05). 특히, 닭고기 부위별 지질의 감마지방산(GLA, gamma-linolenicacid, 18:3n-6) 조성은 대조구와 비교할 때 EPO 첨가구에서 높았다(p<0.05). 이 결과는 육계사료 내 달맞이꽃 종자유를 첨가.급여함으로써 감마지방산이 강화된 새로운 기능성 닭고기를 생산할 수 있음을 시사해 준다.

• 한국수산과학회지
• /
• 제36권2호
• /
• pp.100-106
• /
• 2003

This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was used as the control group where the ratio of raw feed and commercial compound meal is 5:5 $(CP\;30\%,\;CL\;17\%),$ vitamin C added group (VC), vitamin I added group (VE) and vitamin C and I added group (VCE). The supplementary amount of vitamin C and E corresponded to 1000 mg/kg (dry wt.) and 220mg/kg (dry wt.), respectively. Vitamin C was destroyed $50\%$ and vitamin E was destroyed $20\%$ for manufacturing process. After 8 weeks feeding trial, weight gain was $121.9\%$ in MP group, while it ranged from 180.5 to $184.9\%$ in the VC, VE and VCE group. Feed efficiency was $71.\6%$ in MP group, whereas it ranged from 78.7 to $80.6\%$ in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplemented diets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude lipid, crude ash, condition factor (CF), hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was $3.79\%$ in MP group, whereas $4.99\%$ in VC group, $4.55\%$ in VE group and $5.07\%$ in VCE group; Hb was significantly higher in the vitamin added groups than the control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP, VE, VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively. These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did not lead to the synergy effect.