• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.56-61
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• 1995

To examine the effects of gibberelin treatment on seed germination of six wild herbaceous species -Dicentra spectabilis, Aster pekinensis, Agastache rugosa, Elsoholtzia ciliata, Boehmeria tricuspis and Pulsatillia cerrma var. chaerophyiloides - in Kangwon-do, pretreated seeds of five species were sowed on plastic pots with mixed soil(sand：peat moss：perlite： vermiculite, 1：1：1：1, v/v) in 23,April 1995. Seeds were soaked in 25,50 ppm gibberelin solution and ground water during 24 hours. Pretreated seeds of Pulsatiilia cerrma var. chaerophylloides were sowed on nursery soil in 1, June 1995. Seed pretreatments in 31, May 1995 were same method. Seed germination rate was measured and compared among species and treatments. Seed germination rate of Dicentra spectabilis, Agastache rugosa and Elsoholtzia ciliata was differed higly significant among pretreatments ,but those of that of Aster pekinensis, Boehmeria tricuspis and Pulsatillia cerrma var chaerophylloides did not significant at 5% level. Gibberelin pretreatment raised seed germination rate of Dicentra spectabilis and Agastaohe rugosa and proper concentration of GA$_3$ was 25ppm and seed germination rate of Elsoholtzia ciliata was highest at GA$_3$ 50ppm plot. The utilization of wild herbaceous species were discussed.

• Korean Journal of Ophthalmology
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• v.32 no.6
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• pp.433-437
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• 2018

Purpose: Various absorbable anti-adhesion agents have been used to prevent postoperative synechia formation after endonasal surgery. The purpose of this study was to evaluate the anti-adhesion effects of HyFence and Mediclore after endonasal dacryocystorhinostomy (DCR) compared to a mixed solution of hyaluronic acid and sodium carboxymethylcellulose (Guardix-Sol). Methods: In this retrospective study, endonasal DCR and silicone tube intubation were performed on 198 eyes of 151 patients. Three different anti-adhesion adjuvants were applied to the osteotomy site in the nasal cavity after standard endonasal DCR procedures. The subjects were classified into three respective groups: group A (71 eyes, Guardix-Sol 1.5 g), group B (89 eyes, HyFence 1.5 mL), and group C (38 eyes, Mediclore 1 cc). The three groups were evaluated by asking patients about subjective symptoms and by performing lacrimal irrigation tests and endoscopic examinations. Results: There were no statistically significant differences in age, sex, timing of tube removal, or follow-up period among the three groups. There were no statistically significant differences in success rates among the three groups (p = 0.990, 91.5% [65 / 71], 92.1% [82 / 89], and 92.1% [35 / 38], respectively). Conclusions: HyFence and Mediclore are safe and effective adjunctive modalities following endonasal DCR compared to Guardix-Sol. Therefore, these agents can be considered good alternatives to Guardix-Sol to increase the success rate of endonasal DCR in treating patients with poor prognosis.

• Analytical Science and Technology
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• v.21 no.2
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• pp.143-147
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• 2008

U(IV) ion, the valance state of uranium presumed at in a deep-depth disposal of a spent fuel, was prepared and separated from U(VI) ion. In order to prepare U(IV) ion, tests were performed by adding several reducing agents into a uranyl solution or by dissolution of uranium oxide in a mixed acid added with a reducing agent. The valance states of the uranium in the prepared solutions were identified by separating two ions with a Dowex AG 50W-X8 cation exchange resins and measuring the solutions using a laser-induced fluorescence spectroscopy. However, U(IV) and U(VI) were not separated by a Lichroprep Si60 exchange resin in the same separation condition of Pu(IV) and Pu(VI).

• Korean Journal of Food Science and Technology
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• v.27 no.3
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• pp.271-280
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• 1995

These studies examined the production of oligosaccharides by ${\beta}-galactosidase$originated from Aspergillus oryzae and Kluyveromyces fragilis mixed. In addition, heat resistance and acid stability of transgalactosylated oligosaccharides were measured. When ${\beta}-galactosidase$ from A. oryzae, K. fragilis and mixed ${\beta}-galactosidase$ were added to 30%(w/v) lactose solution, maximum production of transgalactosylated oligosaccharides were 26.9%, 37.05 and 27.2%, respectively. The ratios of disacchride, trisacchride and tetrasaccharide in transgalactosylated oligosaccharides were 20.5 : 5.4 : 0.6, 20.4 : 10.5 : 4.2 and 21.0 : 4.1 : 1.9, respectively. Nine different oligosaccharides were recovered with 30% and 40% ethanol fractions. When the 30% ethanol fraction was treated at $150^{\circ}C$ for 10 min more than 90% of oligosaccharides remained stable. More than 90% of the oligosaccharides were stable at $130^{\circ}C$ for 3 min with pH 3.0, whereas there of Kluyveromyces was more than 90% with pH 3.5.

• Korean Journal of Food Science and Technology
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• v.25 no.4
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• pp.327-333
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• 1993

The processing conditions and quality of sardine surimi were examined: Raw sardine meat was separated, washed in 0.2% $NaHCO_3$ and 0.15% NaCl solution, and then dewatered by centrifuge. The dewatered sardine meat was chopped, mixed with 20% emulsion curd (soybean protein : water : refined sardine oil=1:5:2.6), 4% sorbitol, 4% sucrose, 0.2% polyphosphate and 0.1% sodium erythorbate by stone mortar. The mixed sardine meat was frozen with contact freezer, packed in carton box and then stored at $-25{\pm}2^{\circ}C$. The moisture, crude protein and lipid contents of the sardine surimi product was 73.3%, 15.0% and 6.9%, respectively. Fatty acid composition of product consisted of 28.8% of saturates, 24.3% of monoenes and 47.7% of polyenes and the major fatty acids were 16:0, 20:5, 18:1, 22:6 and 16:1. The results of changes in POV, TBA value, fatty acids, texture and sensory score of products during frozen storage showed that lipid oxidation and freeze denaturation of product could be retarded, and flavor enhanced by addition 20% emulsion curd and 0.1% sodium erythorbate. In an attempt to apply sardine surimi in producing surimi-based product, it was concluded that pollack surimi could be substituted with sardine surimi up to 40% without showing any significant changes in texture and taste of surimi-based product.

• Restorative Dentistry and Endodontics
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• v.35 no.4
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• pp.295-301
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• 2010

Recent studies demonstrated that the combination of chlorhexidine (CHX) and sodium hypochlorite (NaOCl) resulted in the formation of a precipitate, para-chloroaniline (PCA). Alexdidine (ALX) is a kind of biguanides like CHX, and has stronger detoxification effect against the bacterial virulence factors such as lipoteichoic acid and lipopolysacchardide compared with CHX. The purpose of this study was to determine whether PCA was formed after chemical interaction between ALX and NaOCl using mass spectrometry. Mass spectrometry was performed for the mixture of five different concentrations of ALX (1, 0.5, 0.25, 0.125, 0.0625%) and 4% NaOCl. Results showed that the peak of PCA was not detected in mixed solutions of ALX and NaOCl in mass spectrometry analysis. The color of mixed solution of ALX and NaOCl after chemical interaction was light yellow to white, but there wasn't any precipitate found.

• Journal of Korean Society of Forest Science
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• v.76 no.2
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• pp.99-108
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• 1987

Half-sib seeds and one-year-old seedlings of Ginkgo biloba were treated with various simulated acid rains (pH 2.0, pH 3.0, pH 4.0 and pH 5.0) to examine the effects of acid rain on seed germination and seedling growth. The seeds were sown in a pot ($4500cm^3$) containing one of three different soils (nursery soil, mixed soil and sandy soil) and the seedlings were grown in the same pots as the seeds. Simulated acid rain was made by diluting sulfuric and nitric acid solution ($H_2SO_4$: $HNO_3$ = 3:1, V/V) with tap water and tap water (pH6.4), and treated by 5mm each time for three minutes during the growing seasons (April to October 1985 and April to August 1986). Acid rain treatments were done three times per week to potted seeds and seedlings by spraying the solutions. The seed germination, seedling growth and physiological characteristics of potted seedlings were compared among three soil types as well as among the various pH levels. The results obtained in this study were as follows: 1. Seed germination of Ginkgo biloba decreased significantly at pH 2.0 level in the field test, and also at the levels of both pH 2.0 and pH 3.0 in the laboratory test, compared to that at control. 2. For two-year-old seedlings, total, top and root dry weights per seedling were significantly different among the three soil types and among the levels of pH, and shoot growth was different only among the levels of pH. 3. For one-year-old seedlings, height and total and stem-branch dry weights per seedling were significantly different among the levels of pH.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.60-71
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• 2014

This study was conducted to identify the potential of rape stalk as a raw material for biorefinery process of rape flower. At first, rape stalk (RS) was immersed in distilled water (DW), acetic acid (AA), oxalic acid (OA), sulfuric acid (SA) and sodium hydroxide (SH) solutions, and the content of reducing sugars liberated from immersed RS was analyzed. Glucose, xylose, arabinose and sucrose were detected varying with the immersion type. In particular, 1% AA-immersion of RS for 72 hr was the most effective conditions to liberate glucose from RS. Secondly, the RS residues were used for elementary analysis and fabrication of fuel pellets. In addition to the solution type, concentration of immersion solutions (0%, 1%, 2%) and immersion time (24, 72, 120 hr) were used as experimental factors. The contents of nitrogen, sulfur and chlorine reduced effectively through the immersion of RS in DW, AA and OA solutions. For properties of RS-based pellets, bulk density and higher heating value of RS-based pellets greatly increased with the immersion of RS, and the qualities were much higher than those of the A-grade pellet of the EN standards. Ash content decreased remarkably through the immersion of RS, and was satisfied with the A-grade pellet standard. Durability was negatively affected by the immersion of RS, and did not reached to B-grade of the EN standard. In conclusion, acid immersion of RS can be a pretreatment method for the production of fuel pellet and bioethanol, but use of the immersed RS for the production of high-quality pellets might be restricted due to low durability of immersed-RS pellets. Therefore, further studies, such as investigation of detailed immersion conditions, fabrication of mixed pellets with wooden materials and addition of binders, are needed to resolve the problems.

• Microbiology and Biotechnology Letters
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• v.9 no.3
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• pp.159-164
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• 1981

Reinforced Calcium-alginate gel entrappment method for enzyme immobilization is described with an example of penicillin amidase from Bacillus megaterium KFCC 10029, a partially constitutive mutant of B. megaterium ATCC 14945. Penicillin amidase recovered from the fermentation broth by adsorption on celite is mixed with alginate and gelatin solution, and cast into a pellet or noodle form by coagulation in calcium salt solution followed by crosslinking with glutaraldehyde. Optimum pH and temperature of the immobilized enzyme preparation were 8.0 and 6$0^{\circ}C$, respectively. Kinetic constants such as Km value and the inhibition constant of 6-APA and phenylacetic acid were 2.6 mM, 7.4 mM and 21.2 mM, respectively. The enzyme leakage from the adsorbent during operation was successfully prevented owing to the increase of physical strength of gel coat. The half lives in a column reactor were 6 and 30 days at the respective temperature of 4$0^{\circ}C$ and 3$0^{\circ}C$, which were the 6-8 fold increased values as compared with those of without entrappment. The results highly recommended the use of reinforced Calcium-alginate gel entrappment method for the enhancement of physical strength and the operational stability of alginate gel entrapped enzyme.

• Journal of Energy Engineering
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• v.17 no.4
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• pp.241-246
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• 2008

Increase of $CO_2$ by using fossil fuels makes mainly global warming and the international efforts to reduce the $CO_2$ emission is being promoted. Absorption process using aqueous alkanolamine solution to remove acid components in the mixed gases has been used commercially. This method was used to remove $CO_2$ in the flue gas in recent years. $CO_2$ Absorption characteristics of several aqueous alkanolamine solutions such as MEA, DEA and AMP was studied by measuring vapor-liquid-equilibrium(VLE) and absorption velocity in this study. VLE measuring equipment, shell and reactor type, was used to acquire VLE data, equilibrium $CO_2$ pressure(${P_{CO_2}}^*$) and time at each pulse gas input. We also acquired the $CO_2$ absorption velocity by measuring the time to arrive the VLE at $40{\sim}80^{\circ}C$ and first gas input. The $CO_2$ absorption capacity of MEA 10wt% solution was higher than two alkanolamine solutions at $40^{\circ}C$ and the equilibrium $CO_2$ loading was 0.5. Absorption capacity was excellent as follows; AMP>DEA>MEA. But absorption velocity was fast as follows; MEA>AMP>DEA. Though good absorbent was considered by many variables, absorption velocity and capacity was more important factor.