• 한국어류학회지
• /
• 제13권1호
• /
• pp.85-88
• /
• 2001

메기 Silurus asotus에서 염색체조작을 효과적으로 하기 위하여, 여러 온도 조건에서의 난할율과 제2, 제3 난할 시간의 평균화에 의한 체세포분열 간격지수 (${\tau}_o$)를 조사하였다. 빠른 난 발생과 비동시적 발생은 고온에서 더욱 뚜렷하게 나타났다. 메기의 체세포분열 간격지수는 $22^{\circ}C$에서 $21.5{\pm}1.4$분, $24^{\circ}C$에서 $18.5{\pm}1.2$분 그리고 $26^{\circ}C$에서 $14.0{\pm}2.1$분이었다. 3가지 수온조건과 ${\tau}_o$ 간에는 Y=-1.85X+21.9 ($R^2=0.9868$, Y는 ${\tau}_o$ 그리고 X는 수온)의 직선회귀가 성립되었다.

• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2003년도 추계학술발표대회 논문요약집
• /
• pp.29-29
• /
• 2003

In order to establish effective procedures for chromosome manipulation in Haliotis gigantea and H. discus, which are of enormous aquacultural potential, temperature-dependent measures of mitotic intervals ($\tau$$_{0}$) were determined. Mitotic intervals ($\tau$$_{0}$) in these abalone were determined by averaging the duration of the first and third embryonic divisions over a range of temperatures from 8 to 26$^{\circ}C$. The relationships of each mitotic interval at two cell ($\tau$$_{I}$), four cell ($\tau$$_{II}$ ), eight cell ($\tau$$_{III}$), sixteen cell ($\tau$$_{IV}$ ) and $\tau$$_{0}$, to temperature (T in $^{\circ}C$) in H. gigantea were log $\tau$$_{I}$ : 176.1-28.3T, log $\tau$$_{II}$ : 199.5-12.4T, log $\tau$$_{III}$ = 236.2-12.2T, log $\tau$$_{IV}$ = 269.3-14.lT and log $\tau$$_{0}$ : 83.1-32.8, respectively. The relationships of each mitotic interval at $\tau$$_{I}$, $\tau$$_{II}$ , $\tau$$_{III}$, $\tau$$_{IV}$ and $\tau$$_{0}$, to temperature in H. discus were log $\tau$$_{I}$ = 104.9-13.8T, log $\tau$$_{II}$ : 138.3-10.5T, $\tau$$_{III}$ : 172.4-10.2T, log $\tau$$_{IV}$ : 211.3-12.2T and log $\tau$$_{0}$=85.6-33.3T, respectively. There were strong, negative correlations between mitotic interval and water temperatures for all ten temperatures in these two species (H. gigantea: Y = -138.75 logX + 341.25, $R^2$ = 0.97; H. discus: Y = -112.33 logX + 255.22, $R^2$ = 0.98, where Y is mitotic interval and X is temperature).d X is temperature).rature).

• Fisheries and Aquatic Sciences
• /
• 제14권4호
• /
• pp.429-433
• /
• 2011

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (${\tau}_0$). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between ${\tau}_0$ and water temperatures at all temperatures studied (Y = -1.225X + 70.05, $r^2=0.988$, where Y is ${\tau}_0$ and X is temperature).

• Fisheries and Aquatic Sciences
• /
• 제13권4호
• /
• pp.278-283
• /
• 2010

Black plaice, Pleuronectes obscurus (Herzenstein), were collected and fertilized to observe egg development, temperature-related cleavage rates, and mitotic intervals (${\tau}_0$). Fertilized egg was demersal, adhesive, and did not contain oil globules. After 1.75 h, the blastodisc formed, and hatching took place 121 h after fertilization at $14^{\circ}C$. The hatched larvae were $3.5{\pm}0.16\;mm$ in total length. At higher temperatures, eggs developed faster and underwent further identical developmental processes. Additionally, ${\tau}_0$ and water temperature were strongly negatively correlated at all temperatures (Y=-2.6981X+98.767, $R^2$=0.9831, where Y is ${\tau}_0$, and X is temperature) for black plaice.

• 한국발생생물학회지:발생과생식
• /
• 제16권4호
• /
• pp.321-327
• /
• 2012

Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup, Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (${\tau}_0$). The fertilized eggs were separative, demersal and light yellowish with $1.5{\pm}0.06mm$ in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min, 60 min and 50 min at $21^{\circ}C$, $24^{\circ}C$, $27^{\circ}C$ and $30^{\circ}C$, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, ${\tau}_0$ were $33.4{\pm}2.08$ min at $21^{\circ}C$, $31.5{\pm}3.06$ min at $24^{\circ}C$, $28.1{\pm}2.11$ min at $27^{\circ}C$ and $26.4{\pm}3.35$ min at $30^{\circ}C$. There were strong negative correlations between the $\tau_0$ and water temperatures at all points studied (Y=-1.13X+58.15, $R^2$=0.98, n=30, where Y is ${\tau}_0$ and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and ${\tau}_0$ data in Korean bullhead.

• 한국발생생물학회지:발생과생식
• /
• 제22권1호
• /
• pp.111-117
• /
• 2018

The objective of this study was to determine the mitotic intervals (${\tau}_0$) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and $24^{\circ}C$). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was $0.09{\pm}0.002mg$. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at $20^{\circ}C$ (p<0.05). The size of the fertilized egg of $18^{\circ}C$ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differences were observed in the other water temperatures group except $18^{\circ}C$ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and $24^{\circ}C$ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between ${\tau}_0$ and water temperature for grass puffer (Y=-1.225X+70.05, $R^2=0.988$, n=10, where Y was ${\tau}_0$ and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be helpful for this species using cleavage frequency and ${\tau}_0$.

• Radiation Oncology Journal
• /
• 제5권1호
• /
• pp.1-11
• /
• 1987

복부 및 골반부의 방사선 조사에서 정상소장의 손상은 대장에 비해 발생빈도는 낮다고 하나 때로는 치명적일수도 있음으로 치료선량을 제한시키는 가장 큰 원인 중의 하나이다. 많은 연구 및 임상보고가 있어 왔으나 동물실험의 경우 대부분이 단일조사 혹은 소수 분할조사에 의한 실험이었으며 인체의 경우 심한 합병증으로 인한 적출물의 생경 혹은 부검소견이 대부분이어서 임상에서와 같은 방법의 체계적인 실험에 의한 보고는 드물다. 이에 저자는 총 396마리의 마우스를 사용하여 전복부에 $2{\times}3cm$의 조사야로 $200{\times}51/wk$로 조사한 후 전 조사량 및 추적기간별로 구분하여 병리조직학적인 소장의 손상정도와 그 회복 및 잔여변화를 관찰하였다. 소낭세포의 분열감소, 융모의 단축, 점막궤양 등의 급성변화는 3,000cGy 이하의 선량에서는 1-2주 이내에 회복되었으나 4,000-5,000cGy군에서 잔여변화가 상당기간 동안 관찰되었다. 소 혈관벽의 비후, 소장점막의 섬유화 및 위축, 만성궤양 등의 후기 면화는 4,000-5,000cGy 조사군의 $2{\sim}3$주 후의 표본에서 현저히 나타나기 시작하여 시간이 경과함에 따라 약간의 회복을 보여주었으나 잔여변화는 15주까지 계속 관찰되었다. 동물실험에서 얻어진 결과를 임상에 직접 적용시키기는 어렵다고 하겠으나 임상에서 흔히 사용되는 선량인 5,000cGy이하에서도 지속적인 만성합병증이 가능함을 보여, 보다나은 임상결과를 위해서는 총선량, 선량율, 분할선량, 조사야 등의 예후에 영향을 줄 수 있는 모든 여건들의 적절한 가감이 필요함을 시사하였다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6923-6928
• /
• 2014

In recent years, mounting evidence has indicated that the CCND1 G870A gene polymorphism, which impacts the mitotic cell cycle, may influence leukemia or non-Hodgkin lymphoma risk. Unfortunately, the previous results were inconsistent. Therefore, a meta-analysis was performed to obtain a more precise estimation of any association. We conducted a search in PubMed, Embase and CNKI covering all published papers up to March, 2014. A total of 9 publications including 10 case-control studies met the inclusion criteria. Odds ratios (ORs) and their 95% confidence intervals (95%CIs) were applied to assess association. The pooled ORs showed significant association in non-Hodgkin lymphoma (comparison A vs G: OR= 1.114, 95%CI=1.053-1.179, p=0.000; homozygote comparison AA vs GG: OR=1.245, 95%CI=1.110-1.396, p=0.000; heterozygote comparison AG vs GG: OR=1.095, 95%CI=1.000-1.199, p=0.05; dominant model AA/GA vs GG: OR=1.137, 95%CI=1.043-1.239, p=0.003; and recessive model AA vs GA/GG: OR=1.177, 95%CI=1.066-1.301, p=0.001). However, there was no association between the CCND1 G870A polymorphism and leukemia risk. In conclusion, the CCND1 G870A polymorphism may increase risk of non-Hodgkin lymphoma, but not leukemia. However, more primary large scale and well-designed studies are still required to evaluate the interaction of CCND1 G870A polymorphism with leukemia and non-Hodgkin lymphoma risk.