• Title/Summary/Keyword: mitochondiral activity

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Effect of lead on mitochondrial activity in rat tussues (랫드의 조직 mitochondria 활성에 대한 납 투여의 영향)

  • Cho, Jong-hoo;Kwon, Oh-deog;Lee, Joo-mook
    • Korean Journal of Veterinary Research
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    • v.36 no.3
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    • pp.565-570
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    • 1996
  • Cardiac, hepatic and renal mitochondria in rats fed lead containing diets were isolated and their activities were studied in terms of NADH oxidation. In normal rats, cardiac and renal mitochondria had similar activities and showed activity values of higher than those in hepatic mitochondria. Cardiac mitochondiral activities in rats fed lead containing diets were increased after 4 weeks of feeding but decreased to activity values close to normal. Renal mitochondrial activities showed a trend of inhibition in all groups fed lead containing diets but were no differenes by feeding periods of 4 and 8 weeks. Feeding of lead containing diets could not be attributed to any changes in the hepatic mitochondrial antivities at experimental doses during 4~8 weeks.

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Subcellular Distribution of Arginase in Leaves of Canavalia lineata (해녀콩(Canavalia lineata) 잎에서 Arginase 활성의 세포내 분포)

  • 유경희
    • Journal of Plant Biology
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    • v.33 no.1
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    • pp.73-80
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    • 1990
  • Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.

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Expression of Human Mitochondiral Aldehyde Dehydrogenase 2 in Mammalian Cells using Vaccinia Virus-T7 RNA Polymerase

  • Kang, Su-Min;Yoo, Seung-Ku;Lee, Ki-Hwan
    • Journal of Microbiology
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    • v.37 no.1
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    • pp.41-44
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    • 1999
  • Human mitochondrial aldehyde dehydrogenase 2 (ALDH2) is mainly responsible for oxidation of acetaldehyde generated during alcohol oxidation in vivo. A full-length cDNA of human liver ALDH2 was successfully expressed using a vaccinia virus-T7 RNA polymerase system. The expressed ALDH2 had an enzymatic activity as high as the native human liver ALDH2 enzyme.

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A Mitochondiral Cytochrome Oxidase I gene based identification of Corbicula ssp. commercially available in South Korea (CO-I 유전자 기반 국내 유통 Corbicula 속 패류의 종 동정)

  • Park, So Young;Kang, Se Won;Hwang, Hee Ju;Chung, Jong Min;Song, Dae Kwon;Park, Hong Seog;Han, Yeon Soo;Lee, Jun-Sang;Kang, Jung-Ha;Lee, Yong Seok
    • The Korean Journal of Malacology
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    • v.32 no.2
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    • pp.127-131
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    • 2016
  • The natives of the genus Corbicula have shown worldwide dispersion in recent times, which has caused great ecological and economic impacts on the introduced ecosystems. The species reported from the genus have been consumed as food and explored for medicine with pharmacological activity. Consequently, the demand of Corbicula sp. in the South Korean domestic market has increased and so also it's associated import to the country. However, due to the absence of identification keys of imported Corbicula, the market is facing confronting situations. We hypothesized that the mitochondrial Cytochrome Oxidase I gene (CO-I) based molecular profiling could be a necessary technique for identification of Corbicula sp. in the South Korea domestic market. The genetic analysis identified both Corbicula japonica and Corbicula fluminea from the market foods. C. japonica and C. fluminea are inhabitants in Korea, but C. fluminea production has decreased in Seomjingang river basin. Therefore, C. fluminea identified from this study, is expected to be imported from China and would have a mixed sales in Seomjingang river side basin.