Recently, a great interest of bio-technology(BT) is concentrated and the image analysis technique for electrophoresis gels is highly requested to analyze genetic information or to look for some new bio-activation materials. For this purpose, the location and quantity of each band in a lane should be measured. In most of existing techniques, the approach of peak searching in a profile of a lane is used. But this peak is improper as the representative of a band, because its location does not correspond to that of the brightest pixel or the center of gravity. Also, it is improper to measure band quantity in most of these approaches because various enhancement processes are commonly applied to original images to extract peaks easily. In this paper, we adopt an approach to measure accumulated brightness as a band quantity in each band region, which Is extracted by not using any process of changing relative brightness, and the gravity center of the region is calculated as a band location. Actually, we first extract lanes with an entropy-based threshold calculated on a gel-image histogram. And then, three other methods are proposed and applied to extract bands. In the MER method, peaks and valleys are searched on a vertical search line by which each lane is bisected. And the minimum enclosing rectangle of each band is set between successive two valleys. On the other hand, in the RG-1 method, each band is extracted by using region growing with a peak as a seed, separating overlapped neighbor bands. In the RG-2 method, peaks and valleys are searched on two vertical lines by which each lane is trisected, and the left and right peaks nay be paired up if they seem to belong to the same band, and then each band region is grown up with a peak or both peaks if exist. To compare above three methods, we have measured the location and amount of bands. As a result, the average errors in band location of MER, RG-1, and RG-2 were 6%, 3%, and 1%, respectively, when the lane length is normalized to a unit value. And the average errors in band amount were 8%, 5%, and 2%, respectively, when the sum of band amount is normalized to a unit value. In conclusion, RG-2 was shown to be more reliable in the accuracy of measuring the location and amount of bands.
Journal of The Korean Society of Grassland and Forage Science
/
v.22
no.4
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pp.265-272
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2002
Forage breeding laboratory of National Livestock Research Institute, R.D.A. has made interspecific hybrids of Lolium multiflorum $\times$ L. pratensis and intergeneric hybrids of Lolium $\times$ Festuca since 1984, and has collected ecotypes of Italian ryegrass since 1991. Growth characteristics of these hybrids and ecotypes were researched, and then these clone lines were named. Among these clone lines, the several clones that have polen fertility, high cold-tolerance, and similar heading time were used for making synthetics, Naehan 6, 7, 8, 9, with polycrossing method in 1997. Field experiments were carried out to compare the mophological and agronomical characteristics and forage productivity and quality of the synthetics with those of Italian ryegrass varieties, Barmultra and Hwasan 101. in Suwon and Yonchun from 1999 to 2000. Heading time of the synthetics were 22th to 24th May that belong to late-mature types to be similar to that of Barmultra and Hwasan 101 in Suwon. The synthetics were 101 to 106 c3n in plant length, medium or thick in thickness of stem, dark peen in leaf color, broad and long in flag leaf, strong in lodging resistance, and excellent in regrowth. Winter survivals of the synthetics were no different from that of Barmultra or Hwasan 101 in Suwon, but better than that of Barmultra or Hwasan 101 in Yonchun where was -10 to -12$^{\circ}C$ of minimum average air temperature in January or February. Dry matter(DM) yields of the synthetics were similar to DM 8,238kg per ha of Barmultra in Suwon, but in Yonchun, were more 7 to 13% than DM 7,291kg per ha of Barmultra. Forage qualities, IVDMD, ADF, NDF and TDN of the synthetics were lower than those of Hwasan 101, but higher than those of Barmultra.
Journal of The Korean Society of Grassland and Forage Science
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v.35
no.2
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pp.81-86
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2015
"Youhan" (Hordeum vulgare L.), a new whole-crop barley cultivar, was developed by the breeding team at National Institute of Crop Science, RDA in 2012. Youhan has the growth habit of III, a light green and mid-sized leaf, hooded and lax-type spikes. The cultivar showed 107 cm of culm length, 641 spikes per $m^2$. The heading date of Youhan was May 1, which is one day later than that of the check cultivar "Yuyeon" in upland, and 2 days earlier than that of Yuyeon in paddy field. The maturation time was similar to check cultivar Yuyeon on June 4 in upland and May 31 in paddy field. In terms of winter hardiness and resistance to lodging and disease, Youhan also performed better than the check cultivar. The average forage dry matter (DM) yield in the regional yield trial was approximately 12.6 ton $ha^{-1}$ and 12.0 ton $ha^{-1}$ in upland and paddy field, respectively, which were 6% and 5% higher than that of the check. The yield also showed 7.3% of crude protein, 26.8% of ADF (acid detergent fiber), 47.8% of NDF (neutral detergent fiber), 67.7% of TDN (total digestible nutrients), and a higher grade of silage quality for the whole-crop barley. Fall sowing cropping of Youhan is recommended only in those areas where the average daily minimum-mean temperatures in January are higher than $-8^{\circ}C$, and it should not be cultivated in mountainous areas of Korea.
The purpose of this retrospective study was to evaluate the level oi alveolar bone support of the erupted Permanent canine through the reconstructed cleft region compared to the contralateral canine on the non-cleft side. This study was limited to children with complete unilateral cleft lip and palate who underwent secondary alveolar iliac bone gvaft and the apices of the erupted canine roots were closed at the time of evaluation. With these criteria the study included 21 children whose average age at the time of bone graft reconstruction was 9.8 years, with a minimum of 12.4 years of age at the time of the evaluation. The study was limited to the use of iliac cancellous bone as the autograft material for reconstruction of the alveolar cleft. Cranial bone graft and other autogenous bone sources were excluded. The periapical radiographs were used to evaluate alveolar bone level of each canine. The percentages of root supported by the bone were established by dividing the amount of root covered with the bone by the anatomic root length. The canine oi the non-cleft side was used as an internal control and the canine on the cleft side was used as an experimental. There was a statistically significant difference in the alveolar bone support ratio between the control ($92.9\%$) and experimental canines ($8.7\%$). An average of $95\%$ level of alveolar bone support was achieved for the experimental canine in comparison to the control canine. Neither the presence of lateral incisor, nor the stage of root development of the canine at the time of the bone graft appeared to have affected the alveolar bone support ratio of the canine after the secondary bone graft.
Journal of the Korean Society of Fisheries and Ocean Technology
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v.41
no.1
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pp.1-8
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2005
In order to find out the environmental factors influencing movement of common grey mullet, Mugil cephalus in funnel net fishing ground of the Dolsan-do, Yeosu southern sea area, the oceanographic factor such as the water temperature, isobath and tidal current were observed respectively, the water temperature was compared with the amount of common grey mullet caught by funnel net. Also, to investigate the movement direction of common grey mullet in same sea area, 160 common grey mullets of body length 22 to 51cm caught at funnel nets of the Dolsan-do southern sea area were marked and then released at 5 positions in 5 times. The results obtained are summarized as follows : 1. The water temperature at the funnel net fishing ground of Dolsan-do in 2002 was ranged from 6.9 to 27.4$^{\circ}C$. The water temperature was displayed a maximum value in August to increase from March and a minimum value in February of the ensuing year to decrease from September. The catches of gray mullet caught by funnel net were generally abundant from March to September, but decreased sharply from October. The optimum range of water temperature for the funnel nets fishing was situated between 15.0 to 25.0$^{\circ}C$. 2. The isobath from 6m to 13m in coast sea set up funnel nets were densely distributed and the depth more than 14m of isobath were widely spreaded to the open sea at Dolsan-do southern sea area. 3. The tidal current of the coast sea set up funnel nets flowed southward and northward along the coast ato ebb and flood tide respectively. The direction of tidal current to the open sea was southeast at ebb tide with the mean speed 43cm/sec, but northwest at flood with the mean speed 25cm/sec. 4. The recapture rate through the experiment duration showed 9.4%. The recapture rate in Gyedong area was very high value with 33.3% as compared with others. The movement of common grey mullet in Dolsan-do southern sea area trended toward a inner bay and north bound mainly.
Jin, Young Seok;Han, Jae Il;Park, Chang Beom;Lee, Chi Hoon;Kim, Byung Ho;Baek, Hea Ja;Kim, Hyung Bae;Lee, Young-Don
Korean Journal of Ichthyology
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v.19
no.1
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pp.8-15
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2007
The morphology of gonad and reproductive cycle of ribbed gunnel (Dictyosoma burgeri) were investigated on the basis of histological observation. The specimens were monthly sampled in the coastal waters of Jeju from November 2001 to February 2003. The ovaries and testis of this species are categorized as cystovarian and lobule type, respectively. The gonadosomatic index (GSI) of female increased in November and maintained high values from December to February. The GSI of male was similar to that of female although it was decreased in February. The reproductive cycle can be grouped into the following successive stage in the ovary: growth (October to November), mature (November to February), spawning (January to February), and degenerating and recovery (March to September). And in the testis, the stage observed were: multiplication (August to November), growth (November to January), mature and spawning (November to February), and degenerating and recovery (January to September). The minimum maturation size of D. burgeri was over 15.0 cm and fecundity ranged from 2,194 to 6,581 eggs. The relationship between the fecundity and fish body was calculated in the fecundity (F) equation as: $F=0.4057TL^{3.1425}$ ($R^2=0.7621$) for total length (TL); $F=149.88BW^{0.9579}$ ($R^2=0.7982$) for body weight (BW), respectively. The fecundity was correlated positively with TL and BW. The histological observations of the gonads suggested that major spawning of this species probably occurs between January to February, when low water temperature ($13{\pm}0.3^{\circ}C$) period.
This study was conducted to develop a stand growth model and a stand yield table for Eucalyptus pellita and Acacia mangium plantations in Kalimantan, Indonesia. To develop a stand growth model, Weibull robability density function, a diameter class model, was applied in this study. In the development of stand growth model by site index and stand age, a hierarchy is generally required - estimation, recovery and prediction of the diameter class model. A number of grow equations were also involved in each process to estimate diameter, height, basal area, minimum or maximum diameter. To examine whether the grow equations are adequate for Eucalyptus pellita or Acacia mangium plantations, a fitness index was analyzed for each equation. The results showed that fitness indices were ranged from 65 to 89% for Eucalyptus pellita plantations and from 72 to 95% for Acacia mangium plantations. As being highly adequate for the plantations, a stand yield table was developed based on the resulted growth model, and applied to estimate the stand growth with midium site index for 10-year period. The highest annual stand growth of Eucalyptus pellita plantations was estimated to be 21.25 $m^3$/ha, while that of Acacia mangium plantations was 27.5 $m^3$/ha. In terms of annual stand growth, Acacia mangium plantations appeared to be more beneficial than Eucalyptus pellita plantations. Also, to estimate commercial timber volume available from the plantations, an assumption that a log would be cut by 2.7 m in length and the rest of the log would be cut by 1.5m was involved. The commercial timber volume available from Eucalyptus pellita plantations was 68.0 $m^3$/ha, 33% from the total stand volume, 203.2 $m^3$/ha. Also 96.7 $m^3$/ha of commercial timbers were available from Acacia mangium plantations, which was 42% from the 232.9 $m^3$/ha in total. Presenting a good information about the stand growth in Eucalyptus pellita and Acacia mangium plantations, this study might be useful for whom proceeds or considers an abroad plantation for merchantable timber production or carbon credit in tropical regions.
Journal of the Korean Society of Fisheries and Ocean Technology
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v.40
no.1
/
pp.86-94
/
2004
This study was conducted to mesh selectivity of Beam trawl for shrimps fishing experiment in the coastal waters around Geomundo, South sea of Korea, during from Oct. to Nov. 2002. The selectivity parameters of big head shrimp (Solenocera melantho) have been studied on the covered con-end method. with mesh of 8, 38, 51 and 61 mm. Selection curves and selection parameters were calculated by using a logistic function S=1/(1+exp-(aCL+b)). The mesh selection master curves were estimated by S=1/(1+exp$^{({\alpha}(CL/M)+{\beta}}$), and the optimum mesh size were calculated with (L/M)50 of master curve. Optimum mesh size and selectivity master curves for the southern rough shrimp (Yrachysalambria curvirostris) and smoothshell shrimp (Parapenaeopsis tenella) optimum mesh size and selectivity master curves were estimated by big head shrimp master curves. The results obtained are summarized as follows : Selection parameters '${\alpha}$' and '${\beta}$' of the master curve for big head shrimp were 8.84 and -5.89, and The selection factor of the master curve (L/M)$_{50}$ was 0.67. The optimum mesh size of minimum length for sexual maturity for big head shrimp was 30.7 mm. Estimated (L/M)$_{50}$ for southern rough shrimp and smoothshell shrimp by using the master curve of big head shrimp was 0.73 and the optimum mesh sizes were 25.5 mm for southern rough shrimp and 16.9 mm for smoothshell shrimp, respectively.
The stability of rice cultivation in Korea is largely depended on climatic conditions, especially, low temperature at the period of early growth stage and after heading. The improvement of cold tolerant varieties and appropriate cultural practices in rice are very effective to minimize the cold damage. This paper is summarized the mechanism and counterplans of cold injury of rice plants. The paddy area having commonly cold injury in Korea is approximately 15, 522ha in 1,709 sites on the national scale. The cold damage at seedling stage in nursery bed appeared to poor germination, leaf discoloration, dead seedlings and seedling rot ect.. At the vegetative stage, the decreased tiller number due to poor rooting and the delayed heading caused by slow growth and panicle differentiation are commonly showed. The cold injury at early reproductive stage appeared to the degeneration of spikelets and rachis - branches, while that at meiosis stage showed to increased sterility due to poor development of pollen and shortened panicle length with delaying heading, therefore the grain yield is largely decreased. The cold damage at heading and ripening stages showed to poor pollination and fertilization, low panicle exsertion, poor grain filling and finally grain quality became low. To minimize the cold injury to rice plants by low temperature, following counterplans would be recommonded ; Improvement of the cold toelrant rice varieties for the regions of midmountains and alpines. Raising healthy seedlings at upland nursery beds and by using of growth regulators such as ABA, Fuchiwang and Tachiace. Soil improvement and organic matter application to reduce cold damage by increasing water and fertilizer holding capacities in the paddy field having commonly cold water and in the place where cold damage is regularly occurred. Appropriate fertilization for raising healthy rice plants to tolerate under low temperature condition. Water management to increase water temperature in the paddy such as depth watering, round channels and polyethylene tubes around the field. Establishment of the optimum cultivation time of rice based on minimum, mean and maximum temperatures at different regions with appropriate rice varieties.
Kim, Taehyoung;Lee, Jinkyun;Chung, Chul-woo;Kim, Jihyun;Lee, Minhee;Kim, Seon-ok
Economic and Environmental Geology
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v.51
no.4
/
pp.359-370
/
2018
The batch and column experiments were performed to overcome the limitation of the neutralization process using the $scCO_2$-water-recycled aggregate, reducing its treatment time to 3 hour. The waste cement mortar and two kinds of recycled aggregate were used for the experiment. In the extraction batch experiment, three different types of waste mortar were reacted with water and $scCO_2$ for 1 ~ 24 hour and the pH of extracted solution from the treated waste mortar was measured to determine the minimum reaction time maintaining below 9.8 of pH. The continuous column experiment was also performed to identify the pH reduction effect of the neutralization process for the massive recycled aggregate, considering the non-equilibrium reaction in the field. Thirty five gram of waste mortar was mixed with 70 mL of distilled water in a high pressurized stainless steel cell at 100 bar and $50^{\circ}C$ for 1 ~ 24 hour as the neutralization process. The dried waste mortar was mixed with water at 150 rpm for 10 min. and the pH of water was measured for 15 days. The XRD and TG/DTA analyses for the waste mortar before and after the reaction were performed to identify the mineralogical change during the neutralization process. The acryl column (16 cm in diameter, 1 m in length) was packed with 3 hour treated (or untreated) recycled aggregate and 220 liter of distilled water was flushed down into the column. The pH and $Ca^{2+}$ concentration of the effluent from the column were measured at the certain time interval. The pH of extracted water from 3 hour treated waste mortar (10 ~ 13 mm in diameter) maintained below 9.8 (the legal limit). From XRD and TG/DTA analyses, the amount of portlandite in the waste mortar decreased after the neutralization process but the calcite was created as the secondary mineral. From the column experiment, the pH of the effluent from the column packed with 3 hour treated recycled aggregate kept below 9.8 regardless of their sizes, identifying that the recycled aggregate with 3 hour $scCO_2$ treatment can be reused in real construction sites.
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