• 한국HCI학회:학술대회논문집
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• 2006.02b
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• pp.608-612
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• 2006

최근에 휴대 전화, 엠피쓰리 플레이어(mp3 player)등 소형기기의 사용이 점점 보편화 되고있다. 소형기기의 경우 화면이 작을수록 휴대하기 용이하다는 이점이 있는 반면에, 작은 화면의 크기로 인해 글자의 크기와 같은 정보제시법에서 제한점이 발생하는 문제점이 있다. 본 연구의 목적은 작은 화면을 오래 사용할 때 초소형 화면에서 가장 좋은 가독성을 보이는 글씨의 크기와 자간을 알아보고자 하는 것이다. $2.5{\times}3.21$cm크기의 화면에서 굴림체 8, 9, 12 point조건과 자간 -100, 0, 100%조건일 때 (포토샵 6.0기준) 에 따른 가독성을 측정하였다. 가독성은 읽기 시간, 이해도, 주관적 가독성(정확도, 읽기의 편함, 주관적 선호도)으로 평가되었다. 9point의 조건이 8point, 12point 조건에 비해서 선호되었고, 글자 크기와 가독성은 비례관계라는 이전의 연구에서 밝혀진 사실들과는 다르다고 할 수 있다. 따라서 본 실험 결과는 향후 작은 화면에서 정보를 제시할 때 디자인 지침으로 활용되어질 수 있다고 사려된다.

• International Journal of Oral Biology
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• v.37 no.2
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• pp.43-50
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• 2012

The use of high throughput screening (HTS) in drug development is principally for the selection new drug candidates or screening of chemical toxicants. This system minimizes the experimental environment and allows for the screening of candidates at the same time. Umbilical cord-derived stem cells have some of the characteristics of fetal stem cell and have several advantages such as the ease with which they can be obtained and lack of ethical issues. To establish a HTS system, optimized conditions that mimic typical cell culture conditions in a minimal space such as 96 well plates are needed for stem cell growth. We have thus established a novel HTS system using human umbilical cord derived-mesenchymal stem cells (hUC-MSCs). To determine the optimal cell number, hUC-MSCs were serially diluted and seeded at 750, 500, 200 and 100 cells per well on 96 well plates. The maintenance efficiencies of these dilutions were compared for 3, 7, 9, and 14 days. The fetal bovine serum (FBS) concentration (20, 10, 5 and 1%) and the cell numbers (750, 500 and 200 cells/well) were compared for 3, 5 and 7 days. In addition, we evaluated the optimal conditions for cell cycle block. These four independent optimization experiments were conducted using an MTT assay. In the results, the optimal conditions for a HTS system using hUC-MSCs were determined to be 300 cell/well cultured for 8 days with 1 or 5% FBS. In addition, we demonstrated that the optimal conditions for a cell cycle block in this culture system are 48 hours in the absence of FBS. In addition, we selected four types of novel small molecule candidates using our HTS system which demonstrates the feasibility if using hUC-MSCs for this type of screen. Moreover, the four candidate compounds can be tested for stem cell research application.