• 제목/요약/키워드: minimal reduction

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Effect of plasmid curing on the 2, 3-dihydroxybenzoic acid production and antibiotic resistance of Acinetobacter sp. B-W (Acinetobacter sp. B-W의 2, 3-dihydroxybenzoic acid 생산과 항생제 저항성에 미치는 플라스미드 제거 효과)

  • Kim, Kyoung-Ja;Kim, Jin-Woo;Yang, Yong-Joon
    • Korean Journal of Microbiology
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    • 제52권3호
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    • pp.254-259
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    • 2016
  • Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at $43^{\circ}C$ was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and $MnSO_4$ containing medium at $28^{\circ}C$ for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli $DH5{\alpha}$ was transformed with plasmid isolated from strain B-W. The transformant E. coli $DH5{\alpha}$ harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli $DH5{\alpha}$ produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid.

Assessment of the Resistance Performance of Hull Appendages Attached to Fishing Vessels Using CFD (CFD에 의한 어선 선체 부가물의 저항성능 평가)

  • Park, Dong-Woo
    • Journal of the Korean Society of Marine Environment & Safety
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    • 제24권2호
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    • pp.267-274
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    • 2018
  • A variety of appendages are attached to coastal and offshore fishing boats to improve stability during navigation or fishing operations (stability performance and roll reduction). These appendages are generally classified into three types, which are shown in Fig. 1. The numerical calculations were conducted for the following eight cases: one case of a bare hull, three cases where a single appendage attached, three cases of a pair of appendages attached, and one case where all three appendages were attached. Table 1 presents the main dimensions of the bare hull and Table 2 describes the main dimensions of the three appendages. The fluid dynamic performance with regard to the three hull appendages was evaluated via CFD for the following cases: each of the appendages attached independently, a pair of appendages attached, and all three appendages attached simultaneously. The No. 1 appendage showed that pressure resistance was proportionally greater than friction resistances. Both Nos. 2 and 3 appendages showed comparable levels of pressure and friction resistances. The correlation between appendages was minimal in the case of pairs and Comparing the cases of the No. 2 appendage attached and all appendages attached, at 11 kn, the EHP showed an difference of 9 %.

An Empirical Study on Clothing Distribution Center to Improve Storage Efficiency : Especially on Hanger Rack Storage According to Distance between Columns (의류 물류센터 보관효율 향상을 위한 실증적 연구 : 행거 랙 보관과 건물기둥 간격을 중심으로)

  • Nam, Hee Dae
    • Journal of the Korea Safety Management & Science
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    • 제21권4호
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    • pp.75-80
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    • 2019
  • Sales of the Korean clothing industry grew to 16.9 percent on-year in 2010, but the growth rate dropped to 3 percent from 2011 to 2016 and the overall market began to slump to 1.1.6 percent in 2017. The competitiveness of clothing companies is also a major sector in logistics capability, and the average logistics cost of domestic clothing companies is 8.3 percent of sales in 2011, higher than the average 8 percent of domestic industries, and 36.4 percent of the total storage cost in 2011, higher than the 28.8 percent share of the total storage cost of domestic companies. As domestic production conditions such as wage hikes and labor disputes worsened in the 1990s, production facilities were rapidly moved overseas, which led many clothing companies to have no production facilities or a minimal production base in Korea and focus on marketing and design capabilities. The total storage capacity and storage efficiency of the logistics center became very important as the products were changed to the form of mass warehousing and small-volume forwarding. Research shows that building column spacing, a model of this research, can affect the amount of hanger rack storage empirically, so for sustainable growth of clothing companies, it is necessary to improve competitiveness in the logistics market by reducing costs and improving efficiency to overcome difficulties in corporate management. Because logistics costs are 8.3 percent and operating profit to sales ratio is 2 percent, it is expected that operating profit will increase by 41.5 percent if logistics costs are reduced by 10 percent. If 10% of storage cost is saved based on storage cost, operating profit is expected to increase by 15% To strengthen the competitiveness of the clothing industry, a reduction in logistics costs is essential. Therefore, the purpose of this study is to provide hints that logistics experts can have empirically small amounts in reducing storage costs through column spacing adjustment of logistics centers that have not been dealt with statistically until now, and to contribute to the continued growth of clothing companies and the development of the domestic logistics industry.

Quality Characteristics of Ginseng Treated by Hot Air Drying after Being Dried using Super-heated Steam (초급속 과열증기를 이용한 인삼의 품질 특성)

  • Kim, Kyung-Tack;Hong, Hee-Do;Kim, Sung-Soo
    • Journal of Ginseng Research
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    • 제33권4호
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    • pp.361-366
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    • 2009
  • This research aims to develop a new drying technology using super-heated steam to reduce the time required for drying ginseng and to enhance its quality. The drying rate of ginseng powder treated with hot-air drying after drying with super-heated steam was about 40% faster than the drying rate of ginseng powder treated with hot-air drying. Minimal difference was found between hot-air drying and hot-air drying after drying with super-heated steam in the general composition of the ginseng powder, such as its crude lipid, crude protein, crude fiber, and ash. Moreover, no difference was found between hot-air drying and hot-air drying after drying with super-heated steam in terms of the ginseng powder's mineral and free sugar contents. The crude saponin contents was 5.43% in the ginseng treated with hot-air drying and 5.4% in the ginseng treated with hot-air drying after drying with super-heated steam. The ginseng powder that was treated with hot-air drying after drying with super-heated steam had a slightly higher redness compared to the ginseng powder that was treated with hot-air drying alone. The number of microorganisms of the ginseng powder treated with hotair drying after drying with super-heated steam showed a reduction of one log cycle in the total bacteria and two log cycles in the yeast molds compared to the ginseng powder treated with hot-air drying alone.

Study on the effect of tail void grouting on the short- and long-term surface settlement in the shield TBM Tunneling using numerical analysis (쉴드TBM터널에서 뒤채움 주입이 지반의 단기·장기 침하에 미치는 영향에 대한 수치해석적 연구)

  • Oh, Ju-Young;Park, Hyunku;Kim, Dohyoung;Chang, Seokbue;Lee, Seungbok;Choi, Hangseok
    • Journal of Korean Tunnelling and Underground Space Association
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    • 제19권2호
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    • pp.265-281
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    • 2017
  • For shallow tunnel constructions, settlement of the ground surface is a main issue. Recent technical developments in shield TBM tunneling technique have enabled a decrease in such settlements based on tunneling with ground deformation controls. For this objective, the tail void grouting is a common practice. Generally surface settlements in a soil of low permeability occur during a tunnel construction but also during a long period after completion of the tunnel. The long-term settlements occur mainly due to consolidation around the tunnel. The consolidation process is caused and determined by the tail void grouting which leads to an excess pore water pressure in the vicinity of the tunnel. Because of this, the grouting pressure has a strong effect on the long-term settlements in the shield tunneling. In order to investigate this effect, a series of coupled hydro-mechanical 3D finite element simulations have been performed. The results show that an increase in grouting pressure reduces the short-term settlements, but in many cases, it doesn't lead to a reduction of the final settlements after the completion of consolidation. Thereby, the existence of a critical grouting pressure is identified, at which the minimal settlements are expected.

Operative Treatment of Tibial Plateau Fractures (경골과 골절의 수술적 치료 결과)

  • Shin, Duck-Seop;Seong, Byeong-Yeon;Kim, Dong-Won
    • Journal of Yeungnam Medical Science
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    • 제18권2호
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    • pp.187-198
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    • 2001
  • Background: The purpose of this study was to evaluate the relationship between variable factors and clinical results following the operative treatment of the tibial plateau fractures. Materials and Methods: The clinical and radiological analysis was performed on 29 cases of the tibial plateau fractures who had been treated with operative treatment and followed up for more than 1 year from January 1991 to December 1997. The analysis of clinical results was performed dividing into age, cause of injury, fracture type of Schatzker classification, associated soft tissue injury and method of operative treatment. Results: According to Schatzker classification, 2 cases(6.9%) were type I, 11 cases(37.9%) were type II, 1 case(3.5%) was type III, 5 cases(17.2%) were type IV, 4 cases(13.8%) were type V, and 6 cases(20.7%) were type VI. In all cases, bony unions were obtained. According to Blokker evaluation, 23 cases(79.3%) of 29 cases were acceptable. Conclusion: We could expect good clinical results if early knee joint mobilization following minimal invasive open reduction and internal fixation could be obtained. Bad clinical results were related with young age group under 30, more than Schatzker classification type IV of high energy trauma and associated injury of anterior cruciated ligment or meniscus.

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Use of Flattening Filter Free Photon Beams for Off-axis Targets in Conformal Arc Stereotactic Body Radiation Therapy

  • Smith, Ashley;Kim, Siyong;Serago, Christopher;Hintenlang, Kathleen;Ko, Stephen;Vallow, Laura;Peterson, Jennifer;Hintenlang, David;Heckman, Michael;Buskirk, Steven
    • Progress in Medical Physics
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    • 제25권4호
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    • pp.288-297
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    • 2014
  • Dynamic conformal arc therapy (DCAT) and flattening-filter-free (FFF) beams are commonly adopted for efficient conformal dose delivery in stereotactic body radiation therapy (SBRT). Off-axis geometry (OAG) may be necessary to obtain full gantry rotation without collision, which has been shown to be beneficial for peripheral targets using flattened beams. In this study dose distributions in OAG using FFF were evaluated and the effect of mechanical rotation induced uncertainty was investigated. For the lateral target, OAG evaluation, sphere targets (2, 4, and 6 cm diameter) were placed at three locations (central axis, 3 cm off-axis, and 6 cm off-axis) in a representative patient CT set. For each target, DCAT plans under the same objective were obtained for 6X, 6FFF, 10X, and 10FFF. The parameters used to evaluate the quality of the plans were homogeneity index (HI), conformality indices (CI), and beam on time (BOT). Next, the mechanical rotation induced uncertainty was evaluated using five SBRT patient plans that were randomly selected from a group of patients with laterally located tumors. For each of the five cases, a plan was generated using OAG and CAG with the same prescription and coverage. Each was replanned to account for one degree collimator/couch rotation errors during delivery. Prescription isodose coverage, CI, and lung dose were evaluated. HI and CI values for the lateral target, OAG evaluation were similar for flattened and unflattened beams; however, 6FFF provided slightly better values than 10FFF in OAG. For all plans the HI and CI were acceptable with the maximum difference between flattened and unflattend beams being 0.1. FFF beams showed better conformality than flattened beams for low doses and small targets. Variation due to rotational error for isodose coverage, CI, and lung dose was generally smaller for CAG compared to OAG, with some of these comparisons reaching statistical significance. However, the variations in dose distributions for either treatment technique were small and may not be clinically significant. FFF beams showed acceptable dose distributions in OAG. Although 10FFF provides more dramatic BOT reduction, it generally provides less favorable dosimetric indices compared to 6FFF in OAG. Mechanical uncertainty in collimator and couch rotation had an increased effect for OAG compared to CAG; however, the variations in dose distributions for either treatment technique were minimal.

Provisioning Anonymous Communication in Ad Hoc Networks (Ad Hoc 네트워크상에서 익명성을 보장하는 방법에 관한 연구)

  • Kang, Seung-Seok
    • Journal of the Korea Society for Simulation
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    • 제15권1호
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    • pp.77-85
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    • 2006
  • The cost of downloading content from the Internet may be costly for mobile device users using its 3G connection, because the 3G connection cost to download data from the Internet is a function of the amount of data downloaded. This paper introduces an approach in which mobile devices, called peers, form an ad hoc network and share their downloaded content with others. As an example, spectators may want to collect/share information about players and game records in a stadium. In an art gallery, visitors may want to retrieve some background information about the displayed work from the nearby ad hoc network. In an outdoor class, a teacher may download today's topic files from the Internet, and all students may share the content with minimal or no cost paid. This is possible if mobile device has both a 3G interface and a wireless LAN interface. If the peers want to improve privacy md discourage traffic analysis when sharing content, this paper describes a low-delay anonymous connection between the sending peer and the receiving peer using two additional peers. Simulation results show that the transmission time overhead of the anonymous connection may increase 50% or less as the number of peers increase or the peers are scattered over the larger area.

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Characterization of Biogenic Amine-reducing Pediococcus pentosaceus Isolated from Traditionally Fermented Soybean Products (전통 장류에서 분리한 Biogenic Amines 저감 유산균 Pediococcus pentosaceus의 분리 및 특성)

  • Oh, HyeonHwa;Ryu, MyeongSeon;Heo, Jun;Jeon, SaeBom;Kim, Young Sang;Jeong, DoYoun;Uhm, Tai-Boong
    • Korean Journal of Microbiology
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    • 제50권4호
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    • pp.319-326
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    • 2014
  • Two bacterial strains, named as LE17 and LE22, were isolated from traditionally fermented soybean products in order to select lactic acid bacteria for the reduction of biogenic amines and harmful bacteria. Both strains were identified as Pediococcus pentosaceus by 16S rRNA sequence analysis and additional biochemical tests. The strain LE17 reduced the amines by 13.7% for histamine and by 25.9% for tyramine, when it grew in minimal synthetic media containing 0.1% (w/v) histamine and 0.1% tyramine at $30^{\circ}C$ for 48 h, while the strain LE22 reduced the amines by 23.7% for histamine and by 15.7% for tyramine. Both strains also had broad inhibition spectra against pathogens. Considering their properties, they could be used as starters for industrial soybean fermentation.

Short Term Outcomes and Prognostic Factors Based on Radiofrequency Thermocoagulation on Lumbar Medial Branches (요추 후지내측지에 대한 고주파열응고술의 단기 성적과 예후 인자)

  • Choi, Byung In;Kweon, Tae Dong;Park, Kyung Bae;Lee, Youn-Woo
    • The Korean Journal of Pain
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    • 제20권2호
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    • pp.116-122
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    • 2007
  • Background: Lumbar zygapophysial joints are a common source of chronic lower back pain and radiofrequency thermocoagulation (RF) of the medial branches (MB) has been shown to be effective at providing substantial pain relief for chronic low back pain. Therefore, we carried out this study to determine the short term outcomes and prognostic factors of RF on the MB of patients with lumbar facet syndrome. Methods: We performed RF in fourteen patients who showed greater than 80% pain relief up to three times after a diagnostic MB block was conducted using 0.3 ml of 0.5% bupivacaine. Using 10 cm curved electrodes with 10-mm active tip, a 60 second, $80^{\circ}C$ lesion was made after electrical stimulation at 50 Hz for sensory and 2 Hz for motor nerve testing. The degree of pain relief was then assessed after 2 weeks, and again after 3 months using a visual analog scale (VAS) and a four point Likert scale. The outcome was regarded as 'success' if at least a 50% reduction in the VAS was observed. Possible prognostic factors between the two groups were also evaluated Results: The success rate was 71.4% (10/14) after three months of follow-up. However, there were transient complications, such as neuritis like syndrome, in 4 patients. In addition, short symptom duration and low minimal voltage (< 0.4 V) for sensory stimulation were shown to be the relevant prognostic factors for a successful outcome. Conclusions: RF may be an alternative to repeated MB block or intraarticular injection for palliation of lumbar facet syndrome. For better outcomes, early diagnosis and strict patient selection should be coupled with efforts to avoid anatomically incorrect RF.