• 대한한의학회지
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• 제26권3호
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• pp.98-109
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• 2005

Objectives : This study was designed to identify the effects of Salvia Miltiorrhiza or antibacterial activity against Streptococcus pneumoniae ATCC 33400. Methods : The ethanol- and water-extracts of more than 80 oriental herbal medicines were investigated by the Kirby-Bauer method to determine their inhibitory effect on growth of S. pneumoniae ATCC 33400 in vitro. Of thorn, Salvia Miltiorrhiza was selected. Results : The ethanol-soluble extract of Salvia Miltiorrhiza showed relatively high antibacterial activity against S. pneumoniae ATCC 33400. However, the water-soluble extract of Salvia Miltionrhiza showed no antibacterial activity. The ethanolic extract was forker fractionated with organic solvents such as hexane, chloroform, and ethyl acetate in that order. Among the fraction tested, the chloroform fraction showed the highest antibacterial activity, when the ethanol-soluble extract of Salvia Miltiorrhiza minimal inhibitory concentration (MIC) was 25${\mu}g/ml$. Conclusions : Further study should be carried out to identity of the cell growth inhibition effects of S. pneumoniae ATCC 33400.

• International Journal of Oral Biology
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• 제42권3호
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• pp.123-128
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• 2017

Recurrent aphthous stomatitis (RAS) is a common oral mucosal disorder for which no curative treatment is available. We previously reported that decreased Streptococcus salivarius and increased Acinetobacter johnsonii on the oral mucosa are associated with RAS risk. The purpose of this study was to identify antibiotics that selectively inhibit A. johnsonii but minimally inhibit oral mucosal commensals. S. salivarius KCTC 5512, S. salivarius KCTC 3960, A. johnsonii KCTC 12405, Rothia mucilaginosa KCTC 19862, and Veillonella dispar KCOM 1864 were subjected to antibiotic susceptibility test using amoxicillin, cefotaxime, gentamicin, clindamycin, and metronidazole in liquid culture. The minimal inhibitory concentration (MIC) was defined as the concentration that inhibits 90% of growth. Only gentamicin presented a higher MIC for A. johnsonii than MICs for S. salivarius and several oral mucosal commensals. Interestingly, the growth of S. salivarius increased 10~200% in the presence of sub-MIC concentrations of gentamicin, which was independent of development of resistance to gentamicin. In conclusion, gentamicin may be useful to restore RAS-associated imbalance in oral microbiota by selectively inhibiting the growth of A. johnsonii but enhancing the growth of S. salivarius.

• 한국응용과학기술학회지
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• 제26권3호
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• pp.350-356
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• 2009

The objection of this research is to conform of practicable possibility and recycling of producing junk after citrus fruits is processed. In the middle of practicable possibility, with verification of antibiosis that tactiling sensibility in a microorganism. With extracting d-limonene oil that have 70${\sim}$90% a component of oil out of junk citrus peel, making certain the about 12000ppm(1.2%) concentration of it. By means of antibiosis property over the a pathogenic bacterium as well as a residence bacterium, considerating the limit of application against daily living supplies needs to antibiosis. Antibiosis effect of a stationing bacterium in the body permanently and by means of antibiosis verification of special bacteria Propionibacterium Acnes that causative skin trouble is selected, in based the antibiotic sencitivity test check up result of minimal inhibitory concentration(MIC).

• 한국식품조리과학회지
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• 제26권2호
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• pp.206-213
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• 2010

In this study, the antibacterial activities of essential oil from Zanthoxylum schinifolium against four Gram-positive bacteria and six Gram-negative bacteria were investigated. The antibacterial activity of the oils was determined using the agar-well diffusion assay, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration). In particular, essential oil from Z. schinifolium showed higher antibacterial activity against Gram-positive bacteria than against Gram-negative bacteria. Essential oil from Z. schinifolium displayed large inhibition zones especially against Bacillus cereus (31 mm). At concentrations between 0 and $20\;{\mu}g/mL$ the oils showed an antibacterial effect against both Gram-negative and Gram-positive bacteria. The minimum inhibitory concentration (MIC) values against nine bacteria ranged from 1.25 to $5\;{\mu}g/mL$. The minimum bactericidal concentration (MBC) values against eight bacterial ranged from 2.5 to $20\;{\mu}g/mL$, except Shigella sonnei. Furthermore, our finding on the antibacterial activities of essential oils from Zanthoxylum schinifolium validated the use of this plant for medical purposes.

• 한국식품과학회지
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• 제33권2호
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• pp.178-183
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• 2001

신갈나무 잎 추출물의 항균력은 추출 온도별로는 $60^{\circ}C$에서 추출시 $30^{\circ}C$와 $90^{\circ}C$에 추출하는 것보다 약간 더 높은 활성을 나타내었으며 추출 용매별로는 에탄올로 추출한 경우가 물 또는 메탄올 추출물보다 대체로 높은 항균활성을 나타내었다. 신갈나무 잎 에탄올 추출물은 그람양성균인 B. cereus, S. aureus 그리고 L. monocytogenes균에 대하여 $62.5{\sim}125\;{\mu}g/mL$ 범위에서 최소저해농도를 나타내었으나 그람음성균인 S. typhimurium과 P. aeruginosa 균은 $250\;{\mu}g/mL$, E. coli O157:H7 균에 대하여는 $500\;{\mu}g/mL$ 이상에서 최소저해농도를 나타내었다. B. subtilis ATCC 6633균과 L. monocytogenes ATCC 19111균에 대한 젖산, 구연산, 초산 및 신갈나무 잎 에탄올 추출물의 최소저해농도는 두균모두 각각 2,500, 5,000, 1,250 및 $125\;{\mu}g/mL$이었으며 이들의 병용처리시 B. subtilis ATCC 6633균에는 효과가 없었으나 L. monocytogenes ATCC 19111균에 대하여는 상승항균효과를 나타내었다. 한편, 각각의 sub-lethal 농도에서 E. coli O157:H7 0019균에 대한 신갈나무 잎 에탄올 추출물과 유기산들과의 병용처리시 두 물질간에는 상승효과는 없는 것으로 나타났다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.65.1-65.1
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• 2008

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.66.1-66.1
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• 2008

• Archives of Pharmacal Research
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• 제22권1호
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• pp.25-29
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• 1999

Psammaplin A, a natural bromotyrosine derivative from an associated form of two sponges (Poecillastra sp. and jaspis sp.) was found to possess the antimicrobial effect on the Gram-positive bacteria, especially on methicillin-resistant Staphylococcus aureus (MRSA). The minimal inhibitory concentration of psammaplin A against twenty one MRSAs ranged from 0.781 to 6.25 ${\mu}g/ml$, which that of ciprofloxacin was 0.391~3.125${\mu}g/ml$. Psammaplin A could not bind to penicillin binding protein, but inhibited the DNA synthesis and the DNA gyrase activity with the respective 50% (DNA synthesis) and 100% (DNA gyrase) inhibitory concentration 2.83 and 100 ${\mu}g/ml$. These results indicate that psammaplin A has a considerable antibacterial activity, although restricted to a somewhat narrow range of bacteria, probably by inhibiting DNA gyrase.

• 약학회지
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• 제42권6호
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• pp.639-641
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• 1998

Minimal inhibitory concentrations (MICs) of Bifidobacterium spp. strains (Bifidobacterium breve K-110, B. breve K-111 and B. infantis K-525) isolated from healthy Korean against antituberculosis agents and fluoroquinolones were determined. From the MICs it was found that Bifidobacterium breve K-110, B. breve K-111 and B. infantis K-525 were susceptible to rifampicin and fluoroquinolenes and resistant to other antituberculosis agents.

• 한국식품영양과학회지
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• 제23권6호
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• pp.1001-1007
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• 1994

In order to develop a natural food preservative, dried salviae miltiorrhizae radix (Salvia miltiorrhiza) was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial sustance from the sample, minimal inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of the initial ethanol extract from the sample was the strongest compared to those of other solvent extracts such as n-hexane, acetone, butanol, methanol and water. the optimum extractingcondition for antimicrobial substance from the sample was shaking extraction for 2 hours at room temperature incase that 10 volumes of absolute ethanol was added to crushed Saliva Miltiorrhiza. The ethanol extract had strong growth inhibition activity against Gram-positive Bacteria (MIC, 3.13-50$\mu\textrm{g}$/ml) such as B. cereus, B, subtilis, L. minocytogenes, S. aureus, Sc. Mutans. Among Grampositive bacteria tested, Bacillus species was the most susceptibile to the extracted substance. The antimicrobial activity of the ethanol extract from the sample was weak to Gram -negative bacteria yeasts, for example MIC for Gram-negative bacteria and yeasts was 0.8mg/ml and 0.4-0.8mg/ml , respectively.