• 대한한의학회지
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• 제26권3호
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• pp.98-109
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• 2005

Objectives : This study was designed to identify the effects of Salvia Miltiorrhiza or antibacterial activity against Streptococcus pneumoniae ATCC 33400. Methods : The ethanol- and water-extracts of more than 80 oriental herbal medicines were investigated by the Kirby-Bauer method to determine their inhibitory effect on growth of S. pneumoniae ATCC 33400 in vitro. Of thorn, Salvia Miltiorrhiza was selected. Results : The ethanol-soluble extract of Salvia Miltiorrhiza showed relatively high antibacterial activity against S. pneumoniae ATCC 33400. However, the water-soluble extract of Salvia Miltionrhiza showed no antibacterial activity. The ethanolic extract was forker fractionated with organic solvents such as hexane, chloroform, and ethyl acetate in that order. Among the fraction tested, the chloroform fraction showed the highest antibacterial activity, when the ethanol-soluble extract of Salvia Miltiorrhiza minimal inhibitory concentration (MIC) was 25${\mu}g/ml$. Conclusions : Further study should be carried out to identity of the cell growth inhibition effects of S. pneumoniae ATCC 33400.

• 약학회지
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• 제43권1호
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• pp.131-133
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• 1999

The bactericidal activities of DW-116, a new fluoroquinolone was estimated by comparing the minimal bactericidal concentrations (MBCs) of it against some Gram-positive and -negative bacterial strains with the minimal inhibitory concentrations (MICs). The MBCs against the test organisms were equal to or two times higher than MICs. The results support that the antibacterial activity of DW-116 is bactericidal.

• Journal of Periodontal and Implant Science
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• 제33권3호
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• pp.341-348
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• 2003

The purpose of this study was to determine the minimal inhibitory concentration (MIC) of cefuroxime axetil,　semisynthetic cefalosporin, for some putative periodotopathogens; F. nucleatum, A. actinomycetemcomitans P. intermedia and P. gingivalis. To investigate the efficacy of cefuroxime axetil, several antibiotics, amoxicillin, metronidazole, and ciprofoxacine, were used as control. The MIC was measured by Murray' s method. The MIC of cefuroxime axetil against some putative microbes, as a single use regimen, was relatively high in comparison with that of the other antibiotics used in this study. The MIC of cefuroxime axetil/metronidazole against some putative microbes, as a simultaneous regimen, was similar to that of the other antibiotics used in this study. The manimal level of cefuroxime concentration in gingival fluid was 9${\mu}$/ml at 36hr after the first dose. In conclusion, within the limited experiment, metronidazole/ cefuroxime axetil therapy of periodontitis may provide a therapeutic benefits in reducing the periodontopathogens.

• 대한의생명과학회지
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• 제3권1호
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• pp.49-54
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• 1997

Pityrosporum spp.은 1952년 Vanbreuseghem가 전풍환자에서 분리한 Pityrosporum spp.를 round form, elongated form 및 oval form으로 구분하고 이중 round form를 Pityrosporum (P.) orbiculare라 하고 oval form를 P. ovale에 해당된다고 하였다. 현재 Pityrosporum 균속에는 P. orbiculare, P. ovale, P. pachydermatis가 있고, 이 중 P. orbiculare와 P. ovale는 호지방성으로 배양시 일반적인 진균배지에 올리브 기름을 첨가하여 37$^{\circ}C$에 배양하면 효모양의 집락을 형성하며 cholesterol, cholesterol ester가 첨가된 배지와 전풍환자에서는 균사를 볼 수 있다. Pityrosporum spp.도 Candida spp. 처럼 효모기 (yeast phase)는 비병원성이고 균사기 (mycelial phase)에는 병원성이 있으며 균의 분리는 전풍환자에서 뿐만 아니라 건강한 성인에서도 90%이상 배양되는 인체에 정상적으로 존재하는 상재균이기도 하다. 이러한 배양 조건 때문에 Pityrosporum균속의 항진균제에 대한 연구는 드물다. 이에 현재 사용되고 있는 ketoconazole, itraconazole, selenium sulfide, sodium thiosulfate의 MIC 검사를 한 결과 ketoconazole은 0.05~0.8$\mu\textrm{g}$ ml$^{-1}$로 itraconazole, solenium sulfite 및 sodium thiosulfate보다 ketoconazole이 MIC가 가장 낮게 나타난다. P. orbiculare의 대한 각 약제의 MIC를 비교해 보면 4종의 약제 전부에서 ketoconazole이 낮았으며, 건강인에서 분리한 균주가 전풍환자에서 분리한 균주보다 MIC$_{50}$, MIC$_{90}$에서 모두 낮았다. P. ovale에 대한 경구용 및 국소용 항진균제의 MIC$_{50}$, MIC$_{90}$에서도 P. orbiculare보다 현저히 낮은 MIC를 보였다.

• 한국식품과학회지
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• 제33권2호
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• pp.178-183
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• 2001

신갈나무 잎 추출물의 항균력은 추출 온도별로는 $60^{\circ}C$에서 추출시 $30^{\circ}C$와 $90^{\circ}C$에 추출하는 것보다 약간 더 높은 활성을 나타내었으며 추출 용매별로는 에탄올로 추출한 경우가 물 또는 메탄올 추출물보다 대체로 높은 항균활성을 나타내었다. 신갈나무 잎 에탄올 추출물은 그람양성균인 B. cereus, S. aureus 그리고 L. monocytogenes균에 대하여 $62.5{\sim}125\;{\mu}g/mL$ 범위에서 최소저해농도를 나타내었으나 그람음성균인 S. typhimurium과 P. aeruginosa 균은 $250\;{\mu}g/mL$, E. coli O157:H7 균에 대하여는 $500\;{\mu}g/mL$ 이상에서 최소저해농도를 나타내었다. B. subtilis ATCC 6633균과 L. monocytogenes ATCC 19111균에 대한 젖산, 구연산, 초산 및 신갈나무 잎 에탄올 추출물의 최소저해농도는 두균모두 각각 2,500, 5,000, 1,250 및 $125\;{\mu}g/mL$이었으며 이들의 병용처리시 B. subtilis ATCC 6633균에는 효과가 없었으나 L. monocytogenes ATCC 19111균에 대하여는 상승항균효과를 나타내었다. 한편, 각각의 sub-lethal 농도에서 E. coli O157:H7 0019균에 대한 신갈나무 잎 에탄올 추출물과 유기산들과의 병용처리시 두 물질간에는 상승효과는 없는 것으로 나타났다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.65.1-65.1
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• 2008

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.66.1-66.1
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• 2008

• 문화기술의 융합
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• 제8권6호
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• pp.615-624
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• 2022

본 연구는 자소자 열수추출물과 압력은 일정하게 유지한 상태로 온도를 조절하여 추출한 3종의 자소자 초임계추출물에 대해 항산화력, 항균활성, biofilm 형성 억제능과 염증매개물질(nitric oxide), 염증성 사이토카인(interleukin-6, interleukin-8)의 생성 억제능을 평가하였다. 자소자 열수추출물과 비교하였을 때 자소자 초임계추출물이 항산화 효과는 떨어졌으나, Staphylococcus aureus(S. aureus)에 대한 minimal inhibitory concentration(MIC)이 월등히 낮은 것으로 확인되었고, biofilm 형성 억제능 또한 더 우수하였다. 염증매개물질과 염증성 사이토카인의 생성도 자소자 초임계추출물이 자소자 열수추출물에 비해 현저히 감소시킬 수 있었다. 따라서 우리는 초임계추출물중 가장 우수한 항균작용과 biofilm 형성 억제, 염증인자 생성 억제 효능을 보인 45℃로 추출한 자소자 초임계추출물이 아토피 피부염 환자의 가려움증과 경표피수분손실을 방지하기 위한 천연 기능성 소재로 적합함을 시사한다.

• 대한한의학회지
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• 제23권3호
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• pp.223-232
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• 2002

Objectives : Methicillin-resistant Staphylococcus aureus (MRSA) CCARM 3251 and S. aureusKCTC 1928 have been known to be resistant to many kinds of antibiotics. The extract of Glycyrrhizae Radix showed antibacterial activity against MRSA and antibiotics-resistant S. aureus. Methods : We examined the effects of the water-soluble extract and the methanol-soluble extract of Glycyrrhizae Radix on MRSA and antibiotic-resistant S. aureus. The methanolic extract was further fractionated with organic solvents such as hexane, chloroform, and ethyl acetate in that order. Results and Conclusions : The methanol-soluble extract of Glycyrrhizae Radix showed relatively high antibacterial activity against MRSA and antibiotic-resistant S. aureus. However, the water-soluble extract of Glycyrrhizae Radix showed no antibacterial activity against MRSA and antibiotic-resistant S. aureus. Among the fractions tested, the chloroform fraction showed the highest antibacterial activity against MRSA and antibiotic-resistant S. aureus. The methanol-soluble extract of Glycyrrhizae Radix minimal inhibitory concentrations (MICs) against MRSA and antibiotics-resistant S. aureus were $5{\;}mg/m{\ell}$ in both. The methanol-soluble extract of Glycyrrhizae Radix was separated using thin-layer chromatography and detected with UV -detector. Further study should be carried out to identify which effects cell growth inhibition of MRSA and antibiotics-resistant S. aureus.

• 한국식품영양과학회지
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• 제23권6호
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• pp.1001-1007
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• 1994

In order to develop a natural food preservative, dried salviae miltiorrhizae radix (Salvia miltiorrhiza) was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial sustance from the sample, minimal inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of the initial ethanol extract from the sample was the strongest compared to those of other solvent extracts such as n-hexane, acetone, butanol, methanol and water. the optimum extractingcondition for antimicrobial substance from the sample was shaking extraction for 2 hours at room temperature incase that 10 volumes of absolute ethanol was added to crushed Saliva Miltiorrhiza. The ethanol extract had strong growth inhibition activity against Gram-positive Bacteria (MIC, 3.13-50$\mu\textrm{g}$/ml) such as B. cereus, B, subtilis, L. minocytogenes, S. aureus, Sc. Mutans. Among Grampositive bacteria tested, Bacillus species was the most susceptibile to the extracted substance. The antimicrobial activity of the ethanol extract from the sample was weak to Gram -negative bacteria yeasts, for example MIC for Gram-negative bacteria and yeasts was 0.8mg/ml and 0.4-0.8mg/ml , respectively.