• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.812-818
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• 2009

This study evaluated the effects of live yeast and yeast cell-wall mannan-oligosaccharide supplementation onperformance and nutrient digestibility during early lactation in cows fed a diet based on a mixture of corn silage and alfalfa hay as forage sources. Eight multiparous Holstein dairy cows (average days in milk, 27${\pm}$6) were used in a replicated 4${\times}$4 Latin square design. Diets contained 45% forage and 55% concentrate on a dry matter (DM) basis and treatments were: i) basal diet without additive (Control), ii) basal diet with 32 g/d of mannan-oligosaccharides (MOS), iii) basal diet with $1.2{\times}10^{10}$ colony forming units per day (cfu/d) of live yeast (Saccharomyces cerevisiae CNCM 1-1077; SC), and iv) basal diet with a mixture of MOS (32 g/d) and SC ($1.2{\times}10^{10}$ cfu/d; MOS+SC). Treatments had no effect (p>0.05) on DM intake and yields of milk, 3.5% fat-(FCM) and energy-corrected milk (ECM), and on milk fat percentage, body condition score and blood metabolites. Compared with the Control, only supplementation of SC resulted in numerically higher yields of FCM (41.9 vs. 40.1 kg/d) and ECM (41.8 vs. 40.3 kg/d), and milk fat percentage (3.64 vs. 3.43%). While the MOS diet had no effects on performance compared to the Control, the combination treatment MOS+SC increased milk protein percentage (p<0.05). Also, the MOS supplementation, both alone or in combination with SC, numerically increased milk fat percentage. The SC supplementation increased apparent digestibility of DM and crude protein while the MOS supplementation did not affect digestibility. Concentrations of total volatile fatty acids (VFA) and ruminal pH were similar across treatments. Overall results indicated that supplementation of MOS produced variable and inconsistent effects on rumen metabolism and performance, whereas SC supplementation improved nutrient digestibility and numerically increased FCM and ECM yields, which could not be enhanced by the combined supplementation of MOS+SC. According to our experimental condition, there was no effect of MOS alone or in combination with SC on dairy cow performance.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.107-111
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• 1995

An experiment was conducted at Pabna milkshed area under Bathan condition during November, 1992 to February 1993 with 16 milking cows fed legume forage with straw supplementation (treated group) and 8 milking cows fed legume forage only (control group). From the study, the results revealed that supplementation of straw with leguminous diet increases the total dry matter intake (DMI) of 11.83 kg/d/cow for the treated group and 11.53 kg/d/cow for the control group. The average daily legume forage intake was $37.39{\pm}8.67kg/d/cow$ and $49.62{\pm}10.57kg/d/cow$ for the treated and control group respectively and the difference was significant (p < 0.05). The results also exhibited that due to the supplementation of straw, the legume forage intake reduced by 12 kg/d/cow. The forage dry matter intake (DMI) kg/d/cow for the treated and control groups were $6.18{\pm}1.44kg$ and $8.38{\pm}1.95kg$ respectively. The milk production was $8.64{\pm}1.15litre/day$ for the treated group which was significantly (p < 0.05) higher than the control group ($7.74{\pm}1.24litre/day$). The average initial body weight of the supplemented and control groups were $338.06{\pm}39.32kg$ and $329.87{\pm}48.03kg$ respectively. Whereas, the final body weight of supplemented group was $344.33{\pm}35.90kg$ and control group was $330.35{\pm}37.28kg$. It may be concluded that straw supplementation with legumes diet could save legume forage for further use as well as increase milk production.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1133-1139
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• 2015

This study aimed to determine the effects of parenteral selenium (Se) and vitamin E supplementation on economic impact, milk yield, and some reproductive parameters in high-yield dairy cows in the dry period and in those at the beginning of lactation. At the beginning of the dry period, cows (n = 323) were randomly divided into three groups as follows: Treatment 1 (T1), Treatment 2 (T2), and Control (C). Cows in group T1 received this preparation 21 days before calving and on calving day, and cows in group T2 received it only on calving day. The cows in the control group did not receive this preparation. Supplementation with Se increased Se serum levels of cows treated at calving day (p<0.05). Differences in milk yield at all weeks and the electrical conductivity values at the 8th and 12th weeks were significant (p<0.05). Supplementation with Se and Vitamin E decreased the incidence of metritis, the number of services per conception and the service period, but had no effects on the incidence of retained fetal membrane. A partial budgeting analysis indicated that Se supplementation was economically profitable; cows in group T1 averaged 240.6$ per cow, those in group T2 averaged 224.6$ per cow. Supplementation with Se and Vitamin E has been found to increase serum Se levels, milk yield, and has positive effects on udder health by decreasing milk conductivity values and incidence of sub-clinical mastitis.

• Korean Journal of Community Nutrition
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• v.3 no.5
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• pp.715-721
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• 1998

This study was performed to investigate how milk supplementation can benefit the elderly by increasing bone density but possibly harming the iron status concomitantly. Forty one elderly subjects over 65 years of age(male : 9, female : 32) participated. All subjects were apparently healthy, home staying and attending meal service for lunch at the welfare center. They were from low income area of Puchon city. One cup of milk per day was served for 10 months. The mean intake of calcium was significantly increased for females after milk supplementation. Males showed significantly increased means of triceps skinfold thickness, suprailiac skinfold thickness and waist circumference. Females showed significantly increased measurements fo three kinds of skinfold thickness, waist circumference, and hip circumference. There were no significant change in the mean bone density of lumbar spine$(L_2~L_4)$, femoral neck, ward's triangle and torchanter, but the proportion of osteopenia estimated by the T score of lumbar spine bone density was lowered from 50.0% to 34.6% for females. The mean Hb level was significantly for males. The proportion of anemia estimated by Hb(<12g/dl), Hct(<36%) and serum ferritin(<15mg/ml) were increased from 17.2% to 51.7%, from 20.7% to 44.8% and from 10.3% to 17.2%, respectively for females. It looks like milk supplementation can effect the intakes of several nutrients considered to be commonly deficient in the Korean diet fo elderly people, increase some anthropometric measurements, and decrease the proportion of osteopenia. However it can have adverse effects on iron status of females.

• Journal of Veterinary Clinics
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• v.29 no.3
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• pp.213-219
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• 2012

The recombinant bovine somatotropin (rbST) has been used for increasing milk production of dairy cows without adverse health effects. This study was conducted to compare effects of supplementation with $Boostin^{(R)}$-250 containing 250 mg of rbST on milk production with those of $Posilac^{(R)}$ and $Boostin^{(R)}$-S. And safety of rbST supplementation on target animals was also observed. Each twenty-five lactating dairy cows were assigned randomly to one of four groups. $Boostin^{(R)}$-250 and vehicle (control) were administered weekly. $Boostin^{(R)}$-S and $Posilac^{(R)}$ were administered two week intervals. Milk yield, milk components, milk somatic cell count, health status, and body condition score of cows were examined. Supplementation with $Posilac^{(R)}$, $Boostin^{(R)}$-S, and $Boostin^{(R)}$-250 induced more milk yield than control group by 2.9 kg/day (12.3%), 4.2 kg/day (17.9%), and 4.1 kg/day (17.4%), respectively. There was a significant difference in milk yield among three rbST treatment groups and control group (${\alpha}$ = 0.05). The rbST supplementation did not increase the incidence of clinical mastitis and milk somatic cell counts. Supplementation with rbST did not significantly affect milk components (milk fat, protein, and solid not fat). The rbST supplementation of the dairy cows after peak milk yield did not cause negative effect on BCS. However, some cows less than 100 days in milking had decreased BCSs after rbST supplementation. In conclusion, milk production in 250 mg of rbST administered cows every week was similar to that of 500 mg of rbST administered cows every 2 weeks. And supplementation of 250 mg of rbST every week could reduce metabolic stress in cows.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.957-967
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• 2016

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.4
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• pp.478-482
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• 2000

Whole cassava (Manihot esculenta, Crantz) crop was harvested about 10-15 cm above ground at 3 months after planting and sun dried for 1-3 days or until the leaves were crispy-dried and the branches and stems were mostly wilted to produce cassava hay. Cassava hay (CH) contained 86.3% DM, 8.9% ash, 23.6% CP, 44.3% NDF, 30.0% ADF, 5.8% ADL, 0.257% condensed tannin and 0.35 mg % HCN, respectively. In addition, CH contained relatively higher amino acid than alfalfa hay especially methionine, isoleucine, leucine and lysine. Ruminal fermentation of CH resulted in high concentrations of $C_2$, $C_3$, and $C_4$ at 72, 17 and 7 mol/100 mole, respectively. A feeding trial was conducted to study on effect of feeding of cassava hay in late lactating dairy cows fed on urea-treated rice straw during the dry season on their intake, ruminal pH, $NH_3$-N, milk yield and compositions. Thirty, Holstein-Friesian crossbred cows in their first lactation were randomly assigned in a randomized complete block design to receive five different dietary treatments: T1=supplementation of concentrate to milk yield at 1:2, T2=supplementation of concentrate to milk yield at 1:2+0.56 kg DM, T3=supplementation of concentrate to milk yield at 1:3+1.3 kg DM CH, T4=supplementation of concentrate to milk yield at 1:4+1.70 kg DM CH, T5=CH fed on ad libitum+small concentrate supplement. All cows received urea-treated rice straw as a roughage source throughout a 80 d feeding trial. The experiment revealed that cassava hay contained high level of protein and minimal level of tannin at 3 months of harvest. Tannin intake ranged from 1.44 to 13.36 g/hd/d and did not affect on urea-treated rice straw intake. Milk yield across treatments were similar (5.4-6.3 kg/hd/d) (p>0.05) but 3.5% FCM was highest in cows received CH at 1.70 kg/hd/d. Feeding of cassava hay resulted in increasing milk fat (4.0 to 4.6%) (p<0.05) and milk protein (3.8 to 5.3%) (p<0.05). Moreover, the use of CH could reduce concentrate supplementation to milk yield from 1:2 to 1:4, respectively, thus resulted in more milk income return.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.12
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• pp.1625-1632
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• 2009

Four lactating Holstein Friesian crossbred cows, with an average initial weight of 450 kg, 48${\pm}$12 days in milk and initial milk yield of 18 kg/h/d, were randomly arranged according to a 2${\times}$2 factorial arrangement in a 4${\times}$4 in Latin square design with 21-d period to investigate the effects of type of total mixed ration (TMR) and type of whole cottonseed (WCS) on intake, digestibility and milk production. The dietary treatments were i) TMR and WCS supplementation at 0.5 kg/h/d, ii) TMR and cracked WCS (cWCS) supplementation at 0.5 kg/h/d, iii) fermented TMR (FTMR) and WCS supplementation at 0.5 kg/h/d, and iv) FTMR and cWCS supplementation at 0.5 kg/h/d. Voluntary feed intake was 15.9, 15.2, 15.4 and 15.6 kg DM/d in dietary treatment 1, 2, 3 and 4, respectively. Digestibility of DM, OM, CP, EE, NDF and ADF were not significantly different among dietary treatments. Ruminal pH, $NH_{3}-N$ and volatile fatty acids in the rumen were also not significantly different among type of TMR or type of WCS. Blood urea-N concentration was not significantly different among dietary treatments. Ruminal bacteria population tended to increase but ruminal protozoa population tended to decrease with supplementation of cWCS, but they were not affected by FTMR. Milk yield and 3.5% FCM were not statistically different among treatments (16.6, 16.2, 17.0, 16.3 kg/d and 18.0, 18.6, 19.9 and 19.0 kg/d, respectively). Milk composition was not significantly different among dietary treatments. However, unsaturated fatty acids in milk fat in cows fed FTMR were lower (p<0.05) than in cows fed TMR. In conclusion, fermentation is a conceivable method to improve the quality of TMR for long-time storage and the cracking method is suitable to release the fat from cottonseed for enhancing fatty acid deposition in milk. Thus, the combination of FTMR and cWCS supplementation would be an alternative strategy to improve performance of lactating cows.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.9
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• pp.1329-1336
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• 2000

Two experiments were carried out to investigate the effect of Murrah buffalo cows pulling sledges under field conditions on milk production and milk composition. In Exp. 1, 24 buffaloes in the fourth month of lactation were used. They were allotted to four treatments according to a $2{\times}2$ factorial arrangement: work or no work, and with or without urea-molasses cake supplementation (700 g/animal/day). Feeds consisted of 20 kg fresh elephant grass (18% DM), 2 kg rice bran per day and rice straw ad lib. The animals worked in pairs three hours per day (work done: $3464{\pm}786kJ/d$) five days a week for three months. Three teams worked in the morning and the others worked in the afternoon in the same day. The following day the working times were switched. In Exp. 2, 16 lactating Murrah buffalo cows in the sixth month of lactation were allotted to two groups (work and no work). They were fed with fresh ruzi grass (Brachiaria ruziziensis) ad lib. supplemented with 2 kg rice bran and 700 g urea-molasses cake. The working regime was similar to that of the first experiment (work done: $3753{\pm}879kJ/d$) and they worked for two months. In the first experiment, there was a small but significant drop (p<0.05) in milk yield from 3.5 to 3.0 kg/day due to work, but there was no supplementation effect. The working buffaloes lost 5.2 kg whereas the non-working animals gained 9.7 kg during the three months (p<0.05). Supplementation increased live weight by 9.9 kg as compared to -5.4 kg for those not supplemented (p<0.05). Milk composition was not affected by the treatments. In the second experiment, daily milk production was similar for both treatments and approximately 3 kg. No significant differences were found in milk composition or in live weight changes for working and non-working groups, respectively. It was concluded that work may cause a reduction in milk yield and a loss of live weight on a poor rice straw diet but that an appropriate supplementation can alleviate this situation.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1377-1385
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• 2011

This study was conducted to determine the nutritive value of live yeast culture (RumiSacc, Saccharomyces cerevisiae) and to investigate its effects on milk yield, milk composition and some blood parameters in lactating cows. Six multiparous Holstein cows were allocated to two groups of three cows and assigned randomly to one of two diets in a cross-over experiment. Daily 50 g RumiSacc was top dressed at the p.m. feeding for the treatment group. RumiSacc supplied a high protein and energy with high organic matter digestibility values (83.35%) determined by in vitro enzymatic analysis. Yeast culture supplementation significantly increased milk yield, tended to increase fat yield, protein yield and lactose yield of milk. Methylated fatty acid level of 18:3 (n-3) in milk fat was increased by yeast culture supplementation. The concentrations of methionine, phenyalanine, tyrosine, tryptophan and taurine were significantly increased with dietary inclusion of yeast culture. Live yeast culture supplementation did not affect other performance characteristics, milk quality characteristics and blood parameters. As a conclusion live yeast culture (RumiSacc, Saccharomyces cerevisiae) had high nutritive value and positive effects on milk production and some milk quality characteristics in lactating cows under field conditions.