• Journal of Plant Biotechnology
• /
• v.44 no.2
• /
• pp.107-114
• /
• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• Journal of Electrical Engineering and Technology
• /
• v.12 no.1
• /
• pp.363-372
• /
• 2017

This paper presents a novel face recognition algorithm based on the deep convolution neural network and key point detection jointed local binary pattern methodology to enhance the accuracy of face recognition. We firstly propose the modified face key feature point location detection method to enhance the traditional localization algorithm to better pre-process the original face images. We put forward the grey information and the color information with combination of a composite model of local information. Then, we optimize the multi-layer network structure deep learning algorithm using the Fisher criterion as reference to adjust the network structure more accurately. Furthermore, we modify the local binary pattern texture description operator and combine it with the neural network to overcome drawbacks that deep neural network could not learn to face image and the local characteristics. Simulation results demonstrate that the proposed algorithm obtains stronger robustness and feasibility compared with the other state-of-the-art algorithms. The proposed algorithm also provides the novel paradigm for the application of deep learning in the field of face recognition which sets the milestone for further research.

• Journal of Engineering Education Research
• /
• v.14 no.3
• /
• pp.45-54
• /
• 2011

The purpose of this study is to analyze performance problems in establishing and improving program educational objectives (PEO) and to set up action strategies for the civil engineering program at the A university. To fulfill the purpose, according to the typical needs analysis model, research problems were defined, current conditions and desired conditions were identified, discrepancies and their reasons were examined, and action strategies were derived. Current conditions and desired conditions were identified by analyzing the A civil engineering program's self study report, conducting surveys and interviews with constituents. After the discrepancies and the reasons were examined, performance problems and field force analysis were conducted to draw short term and long term action strategies to improve PEO. Short term action strategies were to announce PEO to current students, to hold faculty seminars to establish and to improve PEO, to renew the list of constituents regularly, to composite an annual milestone, to define roles of the committees, and to enforce educational opportunity toward industrial advisory board members. For the long term strategies, improvement and documentation of PEO assessment system, collection and analysis of constituents' suggestions, establishment of effective accreditation support system, and arrangement of compensation system for the faculties who are in charge of engineering education accreditation responsibility.

• International Journal of Costume and Fashion
• /
• v.14 no.1
• /
• pp.75-93
• /
• 2014

Facing a mature domestic market and the challenges of the global consumer market, retailers in Taiwan are in pursuit of international development strategies for increasing its market opportunities as one of its key growth strategies. In the global market, the Chinese market becomes Taiwan retailers' main international development milestone due to its similarity of language, culture and historical background with China. Therefore, this research uses case study method based on Eclectic paradigm (Dunning 1981) to explore the various advantages of a Taiwanese children's clothing retailer Les Enphants, which include ownership advantage, internalization advantage, and location advantage. These advantages in turn demonstrate rationales behind Les Enphants' internationalization necessity (Why to Go), selecting China as an expansion target (Where to Go), and management and operation strategies implemented in China (How to Go). Our study highlights a successful entry and expansion model of the Taiwanese children clothing retailer, and may have solid contribution to the practical application of internationalization strategy theory.

• Structural Engineering and Mechanics
• /
• v.72 no.2
• /
• pp.275-291
• /
• 2019

In a super-large underground with "large span and high side wall", it is buried in mountains with uneven lithology, complicated geostress field and developed geological structure. These surrounding rocks are more susceptible to stability issues during the construction period. This paper takes the left bank of Baihetan hydropower station (span is 34m) as a case study example, wherein the deformation mechanism of surrounding rock appears prominent. Through analysis of geological, geophysical, construction and monitoring data, the deformation characteristics and factors are concluded. The failure mechanism, spatial distribution characteristics, and evolution mechanism are also discussed, where rock mechanics theory, $FLAC^{3D}$ numerical simulation, rock creep theory, and the theory of center point are combined. In general, huge underground cavern stability issues has arisen with respect to huge-scale and adverse geological conditions since settling these issues will have milestone significance based on the evolutionary pattern of the surrounding rock and the correlation analyses, the rational structure of the factors, and the method of nonlinear regression modeling with regard to the construction and development of hydropower engineering projects among the worldwide.

• Korean Journal of Food Science and Technology
• /
• v.15 no.3
• /
• pp.302-306
• /
• 1983

The changes in the mechanical model parameters during cooking and subsequent storage, were related to the sensory quality of the noodles. The sensory hardness and chewiness were tested by Milestone method and the overall preference was evaluated by hedonic scale test. Hardness was affected primarily by increasing cooking time and in lesser degree by storage time after cooking. Chewiness was diminished by increasing cooking time and subsequent storage. The preference of wheat flour noodle was not significantly affected by cooking time, while that of wheat·sweet potato starch noodle decreased significantly by excess cooking time. Instantaneous elasticity represented the softness of noodle. The elastic components and viscosity components had significant relationship with the sensory quality of wheat noodle. On the other hand the retardation time was important for the sensory quality of wheat-sweet potato starch noodle.

• Journal of Arbitration Studies
• /
• v.23 no.4
• /
• pp.97-122
• /
• 2013

Vietnam has become an attractive destination for foreign investors, but confidence in the country's legal system to resolve commercial disputes remains low. Reasons include the lack of an independent judiciary, the lack of published court decisions, and a tendency to criminalize civil disputes, among others. As such, arbitration has become a preferred alternative to litigation. On June 17, 2010, the National Assembly of Vietnam passed a new act on commercial arbitration replacing the July 1, 2003 ordinance on commercial arbitration. The new act will take effect on January 1, 2011, and it is widely expected by the Vietnamese legal profession and lawmakers will create a favorable legal framework for the expansion of the arbitration service market in Vietnam. The new act is inspired by the UNCITRAL Model Arbitration Law of 1985 as are most new arbitration laws throughout the world. As opposed to the 2003 ordinance, the 2010 Act allows parties to request interim relief from the arbitrators. Also the new act eliminates the mandate that arbitrators be Vietnamese. The law has addressed the ordinance's shortcomings and reflects international standards. Commercial arbitration law is an important milestone in the improvement process of the laws on commercial arbitration in Vietnam. However, it is still too soon to affirm anything definitely because there remain many obstacles to the activation of arbitration. Rule of law and business cultural factors are important. The leading arbitral institution, VIAC, which is attached to the Vietnam Chamber of Commerce and Industry, is expected to play an important role for boosting the competitiveness of Vietnamese arbitration as an avenue to dispute settlement.

• Journal of Navigation and Port Research
• /
• v.38 no.5
• /
• pp.437-442
• /
• 2014

Ocean Literacy is an understanding of the ocean's influence on you and your influence on the ocean. This research developed the 7 ocean education model courses using ocean literacy based on the analysis of ocean education programs which executed 23 agencies in Busan. These model courses are combined in the type of indoor theory, indoor experience, field study and field experience. Also, this makes the guide map for ocean education in a 76cm*56cm size to distinguish and choose the course easily. This map is the format combined in geological location and tourist attraction spots in Busan, includes education centers, contents, lead time and so on, and it is possible for educatees to handle their preference and seasonality elastically. This map including ocean education model course is a milestone to activate ocean education, and is helpful to reach the goal of ocean education and to lead ocean professionals. In addition, this research presents the development of teaching materials, training aids to complement the weakness of indoor education, the development of cyber education through making video contents as the activation measures of ocean education.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.02a
• /
• pp.159-159
• /
• 2013

Atomically thin graphene is the ideal model system for studying nanoscale friction due to its intrinsic two-dimensional anisotropy. Furthermore, modulating its tribological properties could be an important milestone for graphene-based micro and nano-mechanical devices. Here, we report that the tribological properties can be easily altered via simple chemical modifications of the graphene surface. Friction force microscopy measurements show that hydrogenated, fluorinated, and oxidized graphenes exhibit, 2-, 6-, and 7-fold enhanced nanoscale friction on their surfaces, respectively, compared to pristine graphene. The measured nanoscale friction should be associated with the adhesive and elastic properties of the chemically modified graphenes. Density functional theory calculations suggest that, while the adhesive properties of chemically modified graphenes are marginally reduced down to ~30%, the out-of-plane elastic properties are drastically increased up to 800%. Based on these findings, we propose that nanoscale friction on graphene surfaces is characteristically different from that on conventional solid surfaces; stiffer graphene exhibits higher friction, whereas a stiffer three-dimensional solid generally exhibits lower friction. The unusual friction mechanics of graphene is attributed to the intrinsic mechanical anisotropy of graphene, which is inherently stiff in plane, but remarkably flexible out of plane. The out-of-plane flexibility can be modulated up to an order of magnitude by chemical treatmentof the graphene surface. The correlation between the measured nanoscale friction and the calculated out-of-plane flexibility suggests that the frictional energy in graphene is mainly dissipated through the out-of-plane vibrations, or the flexural phonons of graphene.

• Journal of Biosystems Engineering
• /
• v.39 no.3
• /
• pp.244-252
• /
• 2014

Purpose: The development of an efficient in vitro cell culture device to process various cells would represent a major milestone in biological science and engineering. However, the current conventional macro-scale in vitro cell culture platforms are limited in their capacity for detailed analysis and determination of cellular behavior in complex environments. This paper describes a microfluidic-based culture device that allows accurate control of parameters of physical cues such as pressure. Methods: A microfluidic device, as a model microbioreactor, was designed and fabricated to culture Saccharomyces cerevisiae and Chlamydomonas reinhardtii under various conditions of physical pressure stimulus. This device was compatible with live-cell imaging and allowed quantitative analysis of physical cue-induced behavior in yeast and microalgae. Results: A simple microfluidic-based in vitro cell culture device containing a cell culture channel and an air channel was developed to investigate physical pressure stress-induced behavior in yeasts and microalgae. The shapes of Saccharomyces cerevisiae and Chlamydomonas reinhardtii could be controlled under compressive stress. The lipid production by Chlamydomonas reinhardtii was significantly enhanced by compressive stress in the microfluidic device when compared to cells cultured without compressive stress. Conclusions: This microfluidic-based in vitro cell culture device can be used as a tool for quantitative analysis of cellular behavior under complex physical and chemical conditions.