• Journal of Microbiology and Biotechnology
• /
• v.32 no.7
• /
• pp.938-948
• /
• 2022

Gastric cancers (GC) are generally malignant tumors, occurring with high incidence and threatening public health around the world. Circular RNAs (circRNAs) play crucial roles in modulating various cancers, including GC. However, the functions of circRNAs and their regulatory mechanism in colorectal cancer (CRC) remain largely unknown. This study focuses on both the role of circCOL1A2 in CRC progression as well as its downstream molecular mechanism. Quantitative polymerase chain reaction (qPCR) and western blot were adopted for gene expression analysis. Functional experiments were performed to study the biological functions. Fluorescence in situ hybridization (FISH) and subcellular fraction assays were employed to detect the subcellular distribution. Luciferase reporter, RNA-binding protein immunoprecipitation (RIP), co-immunoprecipitation (Co-IP), RNA pull-down, and immunofluorescence (IF) and immunoprecipitation (IP) assays were used to explore the underlying mechanisms. Our results found circCOL1A2 to be not only upregulated in GC cells, but that it also propels the migration and invasion of GC cells. CircCOL1A2 functions as a competing endogenous RNA (ceRNA) by sequestering microRNA-1286 (miR-1286) to modulate ubiquitin-specific peptidase 10 (USP10), which in turn spurs the migration and invasion of GC cells by regulating RFC2. In sum, CircCOL1A2 sponges miR-1286 to promote cell invasion and migration of GC by elevating the expression of USP10 to downregulate the level of RFC2 ubiquitination. Our study offers a potential novel target for the early diagnosis and treatment of GC.

• Korean Journal of Ecology and Environment
• /
• v.46 no.2
• /
• pp.192-204
• /
• 2013

Fish community structure and inhabit status of endangered fish species were investigated during April to November 2011 in Baebong Stream, Gangwon do, Korea. The number of fish species inhabiting the upper section (Stations 1~3, 5) was 14 and the stream bottoms were mostly composed of cobbles and boulders (Aa-type). Meanwhile, the number of fish species inhabiting the lower section (Stations 4, 6, 7) was 26 and the stream bottoms were mostly composed of pebbles, cobles and sand (Bb-type). A total of 26 species belonging to ten families were found in the stream during the survey period. The dominant and subdominant species were Rhynchocypris steindachneri (31.1%) and Squalidus multimaculatus (13.3%), respectively, and others such as Pungitius kaibarae (11.0%), Zacco koreanus (7.8%), Misgurnus anguillicaudatus (7.0%), Iksookimia pacifica (5.5%) and Z. platypus (5.3%) were followed in the order. Four species of Squalidus multimaculatus, Z. koreanus, I. pacifica and Liobagrus andersoni were endemic to Korea. And, Lethenteron japonica, L. reissneri, Pungitius sinensis and Cottus hangiongensis were endangered fish species in this stream. Oncorhynchus keta, L. japonica and Tribolodon hakonensis were anadromous species, and Plecoglossus altivelis was an amphidromous species. The similarity index based on species composition and abundance clearly divided the fish community structure of the Baebong Stream into two sections fragmented by a weir installed into the stream. It is necessary to install a fish ways on the weirs for a fish migration and movements.

• The Journal of the Korea Contents Association
• /
• v.10 no.8
• /
• pp.426-432
• /
• 2010

A level-set finite-element method is employed to investigate the free surface flow of two conceptual fish passage designs. While one design has a right-angled entrance from the reservoir to the fish passage chute, the other has a curved-shaped entrance. The numerical results are validated with hydraulic experiments through comparison of the free surface location and the pressure distribution in the spillway. It is observed that the right-angled design yields a curved free surface and pressure distribution in the vicinity of the entrance due to large strains, whereas the curved-shaped design yields a smooth flow transition with small strains. The subatmospheirc pressure distributions near the spillway ogee crest for both designs exhibit similar flow patterns. It is concluded that the curved-shaped design is more favorable for fish passage because of the feature of a smooth flow transition with small strains.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.53 no.4
• /
• pp.386-395
• /
• 2017

For scientific research, a number of acoustic surveys using commercial echosounders equipped in fishing vessels were conducted throughout the world; however, few studies were performed in South Korea. Hence, this research is an preliminary study for presenting the application of a sounder from a fishing vessel. The fishing operations using a pair trawler (7 Cheonghae) was conducted in the Northwest-Western sea of Jeju Island from 20 to 23 April, 2016. Substantial impulse noises and attenuated signals were eliminated by the latest algorithms. Acoustic signals were grouped into the fish aggregations and long layer-like signals. The fish aggregations appeared between 30 and 60 m, and long layer-like signals showed the diurnal vertical migration. Energetic, morphological and positional properties of the fish aggregations and layer-like signals were described. The fish aggregations appeared mainly between sunrise and sunset; however layer-like signals tended to be presented regardless of time in consideration of the time series analysis. On the basis of the consignment sales, Scomberomorus niphonius, the target species of F/V 7 Cheonghae, was the highest catch with 4,280 kg (74.6%) and might have appeared in fish aggregations and layer forms.

• Korean Journal of Ecology and Environment
• /
• v.44 no.3
• /
• pp.264-271
• /
• 2011

At the Jangheung multipurpose dam, which is on the Tamjin River, a trapping and trucking operation was established to maintain continuous upstream migration of fish,. To facilitate fish gathering, installation of an effective fishing trap was required. In this study, we evaluated the fish trap, established at the Jangheung dam, using PIT (Passive Integrated Transponder) telemetry. A total of 254 individuals from 15 species were monitored. Among these tagged species, 36 individuals from 6 species (Carassius auratus, C. cuvieri, Zacco temminckii, Z. platypus, Pungtungia herzi, and Pseudobagrus koreanus) were detected; a 14.2% detection rate. C. auratus recorded the highest detection rate of 44.2% while P. herzi was 14.3%. Z. temminckii and Z. platypus showed relatively low detection, 5% and 7.7% respectively. Some of individuals from C. auratus and Z. platypus did not pass through the antenna at the first attempt but were continuously detected on multiple days. There were no statistical differences in body size (total length, standard length and body weight) of individuals that did or did not swim into the trap (Mann-Whitney U test, p>0.05). Fish mainly swam into the trap during outflow of water from the dam (Mann-Whitney U test, p<0.001) and showed a higher detection frequency in daytime than nighttime (Mann-Whitney U test, p<0.001). Thus, for fish movement into the trap, external factors such as outflow from dam and time of day have important roles. Based on detection rate, not all fishes showed upstream migration but represented selective migration. Consequently, the establishment of flexible outflow strategies that take into consideration ecological characteristics of fishes should required for improving the efficiency of fishway.

• Korean Journal of Ecology and Environment
• /
• v.42 no.1
• /
• pp.58-66
• /
• 2009

The telemetry of eight adult manchurian trouts (Brachymystax lenok tsinlingensis) in the upper part of the Nakdong River, which is the southern limit of distribution of manchurian trout on the Korean peninsula, was used to examine migration patterns and evaluate characteristics of over-wintering and the spawning season between December, 2007 and May, 2008. Based on the tracking data, the tagged fish showed a limited migration between adjacent pools, moving only up to $8.6m\;day^{-1}$ during the winter season (December to February). Hydraulic conditions of over-wintering pool areas were, ca. 1m depth, slow moving surface water with areas of sand and gravel. The migration of tagged individuals was successful, moving up to $96.2m\;day^{-1}$ during the spawning season. Two tagged individuals (BL4, BL6) exhibited upstream migration, whereas others showed downstream movements. The timing of upstream migration of the two individuals was consistent with an increasing phases of water level and discharge. The fishes migrating toward the down stream moved to the wide pool areas downstream, where they spent the summer season for the growth.

• Journal of Fisheries and Marine Sciences Education
• /
• v.26 no.2
• /
• pp.421-429
• /
• 2014

We investigated the feeding ecology of juvenile chum salmon (Oncorhynchus keta) during the critical early life stage prey selectivity of juvenile chum salmon during early marine migration in Korean waters at spring 2011. Salmon juveniles and zooplanktons were collected to draw with $20m{\times}5m$ gill net and $300{\mu}m$ mesh zooplankton net at each station on 11th-13th April n 2011. Collected zooplanktons were classified to 5 Phylum, 6 Class, 9 Order 17 Species in this study. Almost 76.4-100% species were classified to Phylum Arthropoda, dominant species was a species out of Hyperia galba of Order Amphipoda, Acartia spp and Paracalanus parvus of Order Calanoida. Collected salmon juveniles were grew up to average 4.7-5.4 cm fork length and average 1.0-1.5 g wet weight in whole station. Fish stomach content (mg/salmon) was heaver to 97.4, 82.4 and 63.2 mg wet weight/salmon in ST 2, 3, 4 than 20.4, 18.9 mg/salmon of ST 1, 5, because there are fish (sand eel, Hypoptychus dybowskii) and Krill (Euphausia) as prey in salmon stomach in ST 2, 3, 4. And ST 2, 3, 4 and 5 were dominated by Amphipoda as Hyperia galba, Themisto japonica and Gammarus sp., but ST 1 was dominated by copepod, because of absence of Amphipoda in the station. Therefore small Amphipoda as Hyperia galba was good prey for just released salmon juvenile in nature.

• Korean Journal of Ichthyology
• /
• v.22 no.4
• /
• pp.238-248
• /
• 2010

We performed field investigations at six stations in Buk Stream of Goseong-gun, Gangwon-do, Korea, from May to September 2009 to understand the stream's fish fauna and community structure. In the survey, 31 species belonging to 20 families were collected. Dominant species by number was Zacoo koreanus (30.0%), and subdominant species were Pungitius kaibarae (9.4%) and Rhynchocypris steindachneri (9.3%). In biomass, the dominant species was Tribolodon hakonensis (35.5%), and subdominant species were Z. koreanus (22.5%) and Cobitis pacifica (7.5%). Eight Korean endemic fish species and two endangered species (P. kaibarae and Pungitius sinensis) were collected. In addition, four anadromous fish species (T. hakonensis, Oncorhynchus masou masou, O. keta, Gasterosteus aculeatus) and two amphidromus fish species (Plecoglossus altivelis altivelis and Gymnogobius urotaenia) were observed. Interestingly, we verified the existence of several fish species in Buk Stream where they have not lived before. Those species were previously reported to live only in rivers that run into the western and southern sea of Korea. Five of those species (Zacoo koreanus, Z. platypus, Silurus microdorsalis, Liobagrus andersoni and Coreoperca herzi) were reported in Buk Stream in the 1980s. The other species (Pungtungia herzi, Pseudorasbora parva, Squalidus multimaculatus, Misgurnus mizolepis, Koreocobitis rotundicaudata, Silurus asotus and Odontobutis interrupta) have been introduced since the 1990s.

• Korean Journal of Ecology and Environment
• /
• v.47 no.spc
• /
• pp.31-38
• /
• 2014

The fish community and upstream of glass eels (Anguilla japonica) in the Imjin River estuary Gyeonggi-do, Korea was investigated from 2008 to 2012. During the surveyed period 43 species belonging to 18 families were collected. Korean endemic species was Microphysogobio jeoni which showed a ration of 0.04% in collected species. Exotic fishes were Carassius cuvieri and Ctenophayngodon idellus, and comprised 0.06% of the total individual number. Among the fish species observed, 17 species of the freshwater fish (40.5%), 6 species of migration fish (14.2%), 8 species of the brackish water fish (19.1%) and 11 species of sea water fish (26.1%). In terms of composition ration, Anguilla japonica (glass eels, 49.8%), Coilia ectens (23.3%), Saurogobio dabryi (8.1%), Repomucenus olidus (3.3%), Lateolabrax maculata (2.9%) and Chelon haematocheilus (2.8%) were found to display high individual number. On the other hand, C. ectens (33.6%), C. haematocheilus (14.2%), Cyprinus carpio (10.5%), Mugil cephalus (9.1%) and S. dabryi (7.6%) were dominated of biomass. The dominant species of individual was A. japonica (glass eels), and subdomint species included C. ectens. The fish biomass catched of fisherman in the Imjin River (Paju-si) were 83.6~240.3 t by each year. The year 2011 and 2012 were decreased rapidly. Among these, M. cephalus (48.0~80.0%), C. carpio (6.7%), Carassius auratus (4.9%), Silurus asotus (3.9%), Takifugu obscurus (2.5%), Anguilla japonica (adult, 2.2%) and Pseudobagrus fulvidraco (2.1%) dominated of biomass. Body weight of glass eels at each year were 0.03~ 1.13 t, average 212.0 kg and 1,325,000 individual by 0.16 g each one individual.

• Korean Journal of Ecology and Environment
• /
• v.39 no.1 s.115
• /
• pp.32-40
• /
• 2006

The Ichthyofauna and fish community were studied in the lower course of Imjin River from April 2003 to October 2004. During the survey period 80 species belonging to 23 families were collected. Twenty-five species (31.1%) of them were Korean endemic species. Exotic fishes were Carassius cuvieri, Lepomis macrochirus and Micropterus salmoides, and they complied 1.3% of total catch. The species of Hemibarbus mylodon was identified as natural monument, and acheilognathus signifer, Pseudopungtungia tenuicorpa, Gobiobotia macrocephala and Gobiobotia brevibarba were endangered species. Migration fish were Coilia ectens, Anguilla japonica, Plecoglossus altivelis and Takifugu obscurus. Dominant species were Zacco platypus (St. 1), Microphysogobio yaluensis (St. 2), Hemibarbus labeo (St. 3, 4) and coilia ectens (St. 5). The lower course of Imjin River was more abundance of species, high diversity and richness, and lower dominance index than those of the lower course of Han River.