• 한국전자현미경학회:학술대회논문집
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• 1995.12a
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• pp.11-12
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• 1995

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1531-1531
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• 2001

A novel technique has been developed to observe the three-dimensional (3-D) distribution of chemical components in biological materials using both automatic sectioning microtome and infrared microscope. The 3-D image was reconstructed based on the relationship between the content and the absorbancy of specified wavelength for chemical components. By using the automatic sectioning microtome, the kernel of rice sample fixed in paraffin was sequentially sliced with the thickness of $5\;\mutextrm{m}$ after pasting the sliced sectional specimens on an adhesive tape. The chemical components of the specimens, which are placed on the X-Y controlling stage with positioning accuracy of ${\pm}10\;\mutextrm{m}$, were analyzed by the infrared microscope. The 3-D images demonstrated that the zonal protein about $200\mutextrm{m}$ in width was observed mainly at the outer parts of a rice particle, and carbohydrates entirely. These images can be observed by choosing arbitrary observation angle. The result indicated that the developed technique could be applied 3-D information to investigate intrinsic chemical components but also residual pesticides as well as bacteria contamination for agricultural produce.

• Korean Journal of Agricultural Science
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• v.50 no.4
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• pp.645-652
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• 2023

The aleurone layer in maize is crucial as it contains essential nutrients such as minerals, vitamins, and high-quality proteins. While most of the maize varieties are known to possess a single aleurone layer, several multi-aleurone layer mutants and landraces have been suggested for hierarchical genetic control of aleurone development. Conventional microscopy analysis often involves using immature seeds or sampling only a portion of the kernel sample, and whole kernel section analysis using a microtome is technically difficult and time-consuming. Additionally, the larger size of maize kernels posed challenges for comprehensive cross-sectional analysis compared to other cereal crops. Consequently, this study aimed to develop an efficient method to comprehensively understand the aleurone layer characteristics of the entire cross-section in maize. Through observations of diverse maize genetic resources, we confirmed irregular aleurone layer patterns in those with multiple aleurone layers, and we discovered a landrace having multiple aleurone layers. By selectively identifying genetic resources with multiple aleurone layers, this method may contribute to efficient breeding processes in maize.

• Korean Journal Plant Pathology
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• v.12 no.4
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• pp.471-475
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• 1996

콩나물의 생육부진과 부패는 콩나물 재배에 중요한 제한요인이다. 콩나물 부패 병원 진균으로 Fusarium moniliforme, F. oxysporum과 F. solani를 분리 동정하였다. 이들은 종자 발아 후 3∼5일이 지난후 콩나물의 중간 이하에 연한 갈색의 외부 병징을 만들며 발아 후 6∼7일 경에 병징이 더욱 진전하여 진한 갈색의 외부 병징을 나타냄으로써 콩나물에 대한 병원성이 인정되었다. Hand-section과 microtome-section에 의한 병태 조직학적 관찰에서 F. oxysporum 접종 후 4일이 경과하여 초기 병징을 보인 조직에서 균사가 피층, 세포간극, 목부, 사부, 수에 분포하고 있음이 관찰되었다.

• Proceedings of the KACD Conference
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• 2001.11a
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• pp.574.2-574
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• 2001

The objective of this study was to evaluate the effect of EDTA irrigant depending on application times and temperatures. 31 human mature extracted teeth with a single canal and a closed apex were sectioned with microtome in 3mm thickness. They were distributed randomly into 6 groups of 10 specimens each and control group of 2 specimens. Each specimen was prepared with $GT^{TM}$ rotary file (Dentsply, Maillefer Co., Swiss) and irrigated with 3ml sodium hypochlorite every minute. Then smear layer wasn removed with EDTA solution ($PULPDENT^{\circledR}$, PULPDENT Co., USA.) except two control specimens. Specimens of each group were irrigated with 17％ EDTA.(omitted)

• Applied Microscopy
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• v.1 no.1
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• pp.27-34
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• 1969

The compound eyes of the adult Drosophila melanogaster were. fixed in 1.25 per cent glutaraldehyde and 1 per cent osmium tetroxide buffered with sodium cacodylate at pH 7.2. Double fixed specimens were dehydrated using the alcohol series and embedded in Epon 812. They were sectioned with porter blum and JUM 5B ultra-microtome and then stained with lead hydrooxide and uranyl acetate. All thin sections were examined with Hitachi HS-7 or HU-11 electron microscope. The rhabdomere of the compound eye is composed of numerous microvilli packed, arranged, and projected from inner edge of each retinal cell. Each microvillus consisted of a centrum, about $82{\AA}$ in diameter, surrounded by the substances, about $105{\AA}$ in width, which were bounded with double membrane about $44{\AA}$ in thickness. In each inner edge of the microvilli, there was a cylinder, about $175{\AA}$ in diameter, in parallel with retinal cells, which contained a cylindrical axis about $583{\AA}$ in diameter. The surface of the outer edges .of .the microvilli was bounded with reticullar substances about $500{\AA}$ in thickness.

• The Korean Journal of Zoology
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• v.35 no.4
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• pp.456-465
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• 1992

The 94 KDa glucose-resulsted Protein (SH 94), one of stress Proteins, is a Ca2+-binding protein in the endoplasmic reticulum (ER). In this study, the possible effect of Ca2+ on the native conformation of grp 94 was examined. When the purified grp 94 was analyzed by Sel filtration in the presence of either EGTA or CaCl2, it was eluted with apparent molecular weight (MW) of 100 KDa in both cases. When similarly analyzed with microtome or cell Ivsate, however, srp 94 was eluted with apparent IW of 200 KDa in the presence of E6TA, while with apparent MW of 100 KDa in the presence of CaCl2, indicating possible association of grp 94 with one or more other proteins in the absence of CaCl2. Consequently, immunoprecipitation with anti-grp 94 was carried out to determine which proteins specifically interact with grp 94. It is sho%un that srp 94 may interact, in a Ca2+_dependent manner. with other proteins including BiP (grp 78) which is also a stress protein in the ER.

• Korean Journal of Plant Resources
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• v.28 no.3
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• pp.350-356
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• 2015

Abeliophyllum distichum is a monotypic taxon of Oleaceae and endemic to Korea. A comprehensive study on embryogeny and fruit and seed coat ontogeny in Abeliophyllum was carried out via microtome and light microscopy. The fertilization occurs during mid– to late April and embryo matures by early July. The embryo development follows the general fashion from globular embryo – transition embryo – heart shaped embryo – torpedo embryo – walking-stick embryo to mature embryo. The pericarp clearly differentiates into three histological zones: exocarp, mesocarp, and endocarp. The young seed comprises 10-12 cells thick seed coat and the mature seed coat comprises an exotesta, 6-8 mesotesta and an endotesta. Any crystals, phenolic-like compounds, idioblasts, and the sclereids are not found in pericarp as well as seed coat. An overall development confirms Solanade type of embryogenesis in Abeliophyllum. The endocarp becomes more prominent in mature fruit and all the layers of endocarp are highly lignified. On the basis of mechanical layer the seed coat is of exotestal type.

• Transactions of the Korean hydrogen and new energy society
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• v.23 no.3
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• pp.256-263
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• 2012

We studied the degradation phenomenon of Pt catalyst in PEMFC. We used the electron microscope analysis technique including the ultra-microtome pretreatment method, FEG-SEM and TEM analysis methods for analysis of Pt nanoparticles. The Pt catalyst degradation is observed not only in electrode site but also in membrane site. We investigated these various degradation phenomena. The cathode electrode layer thickness is reduced. The size of the catalyst is increased much larger than initial size in membrane site. The catalyst moved from electrode layer to the electrolyte membrane. The rounded shape of catalyst was changed to the polygon. As a result, we found that the catalyst degradation processes of migration and coarsening occurred by the followings mechanisms; (1) dissolution of Pt ; (2) diffusion of Pt ion ; (3) Pt ion chemical reduction in membrane; (4) Coarsening of Pt particles (Ostwald ripening) ; (5) polygon shape change of Pt by {111} plane growth.

• The Korean Journal of Quaternary Research
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• v.17 no.1
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• pp.1-6
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• 2003

One hundred fifty six pieces of woods were excavated at the muddy sand layer (post-glacial age: about 8,800 bp) above the upper peat layer from Sarori, Chungwongun, Korea in the central Korea. Due to the deteriorated structure of peat woods, all samples were embedded in PEG(polyethylene glycol) 2000 and then sectioned using a rotary microtome. Only two species were found; Alnus spp.(95%) and Ulmus spp.(5%). No conifers were found. Species composition indicates that the climate condition of central Korean peninsula around 8,800 bp was little wetter condition. The sampled region at the Sarori appeared to be a swamp or riverside.