• The Korea Journal of Herbology
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• v.21 no.4
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• pp.183-187
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• 2006

Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing powder forms of these materials. Methods : To make the specimen, the samples were grinded, and the powder was mounted by a solution of Glycerin, Acetic acid and Water at the rate of 1 : 1 : 1. The specimen was mounted by optic microscope, photographs were taken and the characteristics were sketched in lines. Result : 1. Korean Osterici Radix was yellow-brown, but Chinese Notopterigii Rhizoma et Radix was dark-brown. 2. Oil ducts were mostly broken, and most of secretory cells were narrow and long, including yellow or light yellow secretion and starch grain. 3. Parenchyma cells were rectangular, and southern type of Osterici Radix and Chinese Notopterigii Rhizoma et Radix include lots of starch grain in parenchyma cells, while northern type of Osterici Radix and wild Osterici Radix include a little. 4. Vessels were usually reticulate vessel, and the diameter of the vessels of Korean Osterici Radix was $25{\sim}88{\mu}m$, while that of the vessels of Chinese Notopterigii Rhizoma et Radix was $13{\sim}52{\mu}m$. 5. The surface of accessory cells were light brown with the shape of irregular polygon. Conclusion : Southern type of Osterici Radix, nothern type of Osterici Radix, wild Osterici Radix, and Chinese Notopterigii Rhizoma et Radix were all alike, except the amount of starch grain in parenchyma cells.

• The Korean Journal of Cytopathology
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• v.1 no.2
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• pp.164-169
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• 1990

Carcinsarcoma is an uncommon pulmonary malignancy characterized by carcinmatous parenchyma and sarcomatous stroma. The cytologic, immunohistochemical and ultrastructural features of a case of pulmonary carcinosarcoma suspected by fine needle aspiration cytology is presented. Only bizarre spindle cells arranged in loose groups, in microtissue fragments and in a dissociate fashion were present on the aspiration smears. They were markedly positive for vimentin. The epithelial component was not found, which was probably due to marked paucity of carcinomatous component that was proved by histologic examination of the resected tumor. The diagnosis of pulmonary carcinosarcoma should be conidered whenever poorly differentiated epithelial ceil groups with a malignant mesenchymal component set in a myxoid background are seen in a pulmonary cytology specimen.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1993.05a
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• pp.89-89
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• 1993

The laryngeal epithelial cell kinetics of 26 laryngeal lesions(invasive squamous cell carcinoma 14, epithelial hyperplasia 5, laryngeal nodule 7) were studied by immunehistochemical analysis with the monoclonal antibody Ki-67, which reacts with nuclear antigen in proliferating cells using paraffin embedded tissue. For DNA analysis, touch implint with fresh biopsy specimens were stained with feulgen and analyzed by image analyzer in 22 cases. 1) The proportion of Ki-67-positive cells were 32.65$\pm$ 11.59% in invasive squamous cell ca, 20.14$\pm$3.38% in epithelial hyperplasia lesion and 11.66$\pm$3.02% in laryngeal nodule. 2) DNA aneuploidy was found in 7 cases of 10(70%) invasive squamous cell carcinomas, 2 cases of 5(40%) epithelial hyperplasia lesions and all cases of laryngeal 3) Proliferation index(S phase+G2/M phase) show 23.42$\pm$11.33% in squamous cell carcinoma, 13.09$\pm$ 10.90% in epithelial hyperplasia lesion and 4.50$\pm$1.19% in laryngeal nodule. As the results, measuring the DNA content from touch imprint method together positivity of Ki-67 antibody from the microtissue during the laryngeal microscopic surgery, cell kinetics can be assessed as an effort of deciding the prognosis and provide a key to the management of precancerous lesions.