• Title/Summary/Keyword: microscopic examination

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Characteristics of Grinding Force and Surface Roughness by CBN and WA Wheel (CBN과 WA숫돌에 의한 연삭에서 연삭력과 표면거칠기 특성)

  • 하만경;곽재섭;양재용;정영득;심성보;류인일
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.11 no.1
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    • pp.26-31
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    • 2002
  • In this study an experimental investigation was conducted to find the grinding characteristics of ceramics, STD11 and STS304 materials. The grinding force and the microscopic observation of the workpieces were obtained in surface grinding. Grinding characteristics of ceramics were inspected through the microscopic examination the cutting force, and the surface roughness. It has been found that the finding force of ceramics is relatively low as compared to that of steels and that CBN wheel has an excellent performance. The surface roughness was measured according to the feedrate and the depth of out.

Black Hair Follicular Dysplasia in a Shih-tzu Dog (시츄견에서 발생한 검은색털 모낭 이형성증)

  • Kim, Ha-Jung;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.32 no.6
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    • pp.527-529
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    • 2015
  • A 7 year-old intact female black spotted and white hair Shih-tzu dog visited for screening of vital condition and was suspected multifocal alopecia on physical examination. On dermatologic examination, alopecia with patches on black spot area, generalized seborrhea sicca, and bacterial infection were detected. Additionally, melanin clumping in the hair shafts on the alopecic area was detected by microscopic examination. Based on the signalment and dermatologic examination, black hair follicular dysplasia was diagnosed. To our best knowledge, this the first case report of black hair follicular dysplasia in Shih-tzu.

AN EXPERIMENTAL STUDY OF THE IRRADIATION EFFECTS ON THE MICROVASCULATURE OF THE RAT SUBMANDIBULAR GLAND (방사선 조사가 백서 악하선 미세혈관계에 미치는 영향에 관한 실험적 연구)

  • Choi Karp Shik;Choi Soon Chul;Park Tae won
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.22 no.1
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    • pp.43-53
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    • 1992
  • The purpose of this study was to investigate the effects of irradiation on the microvascular structure of the submandibular gland in rats. For this study, 110 male rats were singly irradiated with the dose of 10Gy or 20Gy to their neck region by 6MV X-irradiation and sacrificed on the 1st, 3rd, 7th, 14th and 28th day after irradiation. The author observed distribution and structural changes of the microvasculature in rat submandibular glands using a scanning electron microscope by forming vascular resin casting. The author observed ultrastructural changes of the endothelial cells using a transmission electron microscope, and also histologic changes using a light microscope at Hematoxylin and Eosin staining and PAS staining process. The results of the irradiation effects on the microvasculature in rat submandibular gland were as follows: By light microscopic examination, the dilation of small vessels were observed until the 7th day after irradiation. After then, the vascular constriction and decrease in number of small vessels were noticed. Changes were greater on 20Gy irradiated group than on lOGy irradiated group. The reaction to PAS staining at acinar cells was decreased just after irradiation, but gradually recovered with days. There was no specific difference between two irradiated groups. By scanning electron microscopic examination, general findings on the two irradiated groups were similar. The dilation of conduits and meandering were observed on the 3rd day after irradiation. Decrease of capillary density and blunt ended small vessels were appeared on the 7th day after irradiation. After that, findings of the tortuous and twisted vascular running and coarseness of capillary lumen were increased. Changes were greater on 20Gy irradiated group than on l0Gy irradiated group. By transmission electron microscopic examination, increase of the formation of cytoplasmic process was observed on the 3rd day after irradiation. After that, swelling of endothelial cell and bridge formation of cytoplasmic processes were also observed, but destruction of endothelial cell and loss of basement membrane were observed only on 20Gy irradiated group on the 28th day after irradiation.

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EARLY IRRADIATION EFFECTS ON THE ULTRASTRUCTURE OF THE CAPILLARY ENDOTHELIAL CELL IN THE RAT SUBMANDIBULAR GLANDS (방사선조사에 따른 백서 악하선내 혈관 내피세포 미세구조의 조기변화)

  • Ryu Jung-Soo;Sohn Jaong-Ick;Baa Yong-Chul;Choi Karp-Shik
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.25 no.1
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    • pp.71-87
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    • 1995
  • The purpose of this study was to investigate the early irradiation changes on the ultrastructure of the capillary endothelial cell in the rat submandibular glands. For the study, 110 Sprague-Dawley strain male rats were singly irradiated to their neck regions with the doses of 2Gy, 5Gy, and 10Gy by 6MV X -irradiation, and sacrificed on the 3 hours, 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after irradiation. The authors observed the histologic and ultrastructural changes of the capillary endothelial cell using the light and electron microscopes. The results were as follows: I. In the light microscopic examination, the capillary dilation was observed on the 6 hours group and the capillary density was slightly increased on the 12 hours group after 2Gy and 5Gy irradiation. And luminal size and capillary density were decreased on the 3 days and the 7 days groups after irradiation, after then, they were recovered. But capillary density was still decreased on the 14 days group after 10Gy irradiation. 2. In the transmission electron microscopic examination, the mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed on the 3 hours group after irradiation. After then, endothelial swelling, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous pinocytotic vesicles were observed after the 1 day group after irradiation. Thickened basal lamina and numerous pinocytotic vesicles were still observed until the 7 days group after irradiation. These changes were recovered to normal on the 14 days group after 2Gy and 5Gy irradiation, but not after 10Gy irradiation. 3. In the scanning electron microscopic examination, the dilation of conduits and constriction, and meandering were observed on the 1 day group after 10Gy irradiation. These changes were observed with increased coarseness of the surface of the vascular resin casting on the 3 days group after irradiation. 4. From the above results, endothelial swelling, proliferation of cytoplasmic process, and thickening of the basal lamina appeared before the 6 hours group after irradiation. And these changes may also induce the increase of the capillary number and luminal size, after then, capillary permeability was increased via the increase of the number of pinocytotic vesicles. The changes were observed earlier and more apparent with the increase of the irradiation doses under the dose of 10Gy irradiation.

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Chemical Preservation Methods of Urine Sediment for Quality Control (정도관리를 위한 요침사의 화학적 보존방법)

  • Cho, Soung Suck;Kim, Myong Soo;Shin, Kyung-A
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.4
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    • pp.359-365
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    • 2017
  • There is greater standardization of quality control for microscopic examination of urine than for physicochemical test. In this study, we investigated whether it is possible to control the sediment accuracy by microscopic examination through the real thing by preserving the essential sediment with glutaraldehyde, which is required for the rationality of sediment quality control. A urine specimen was prepared using 2.5% glutaraldehyde as a preservation solution. Samples treated with urine preservatives confirmed the morphological deformation of the cells for four weeks at intervals of one week and confirmed whether they should be preserved for 4 weeks thereafter. After preparing the required sediment slide, two more slides were produced; one was stored in a refrigerator for, and the other was stored at room temperature. The morphological deformation of the specimen was confirmed. Glutaraldehyde has the effect of preserving the refrigerated essential sediments and storing them for up to 8 weeks, refrigerated storage after slide production, stabilized by 3 days. Moreover, after treatment with preservatives, the production of the slide and comparison between the measured values between the laboratories and examiners showed a low consistency. In conclusion, we showed that the urine sediment components can be preserved, and it can be used for quality control and education through real objects.

AN EXPERIMENTAL STUDY ON THE HEALING PROCESS OF TOOTHASH, PLASTER OF PARIS AND AUTOGENOUS BONE COMPOSITE GRAFTING IN DOGS (치아회분과 석고혼합제재 매식과 자가골 동시 이식후 치유과정에 관한 실험적 연구)

  • Yeo, Hwan-Ho;Jung, Jae-Hun;Lee, Sang-Ho;Kim, Heung-Jung;Kim, Young-Kyun;Lim, Seung-Cheul;Sul, In-Tak
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.22 no.1
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    • pp.1-14
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    • 2000
  • The purpose of this study was to evaluate the efficacy of adding autogenous bone to the toothash-plaster mixture in the healing process of bone. Full-thickness round osseous defects with the diameter of 20mm were made at the calvarial bone of adult dogs (n=19) bilaterally, which were thought to be critical size defect. The right defects were repaired with the toothash-plaster mixture plus autogenous bone (compressed volume 0.3cc) and the left defects with only toothash-plaster mixture. At 2-, 4-, 8-, 12- and 20- week after implantation, dogs were sacrificed and evaluated the osseous healing of bony defects clinically, radiographically, and microscopically. The results were as follows; 1. At the clinical observation, the wound healed very well without any problem except severe swelling in the early period after operation. Slight depression was recognized at the both sides when the portions of cranial defect were palpated. 2. There were statistically significant differences between toothash-plaster mixture groups and autogenous bone added groups at the same period, and among the groups in the bone density of the digital radiograms (P<0.001). There was a tendency that bone density was increasing with time. 3. In light microscopic examination, new bone formation was more active in the autogenous bone added groups than toothash-plaster mixture groups at the early period after implantation but there is little difference at 20-week after implantation. 4. In fluorescent microscopic examination, the fluorescent band could be observed at the area of active bone formation and the band was more distinct in the autogenous bone added groups then toothash-plaster mixture groups. 5. In transmitted electron microscopic examination, organelles such as rER, Golgi complex and secretory granule and osteoblast were observed. In summary higher volume ratio of autogenous bone is needed to improve the bone healing in that there is little difference between toothash-plaster mixture group and autogenous bone added group at the 20-week after implantation in spite of new bone formation was more active in the autogenous bone added groups than toothash-plaster mixture groups at the early period after operation.

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A Case of Pulmonary Fibrosis with Microscopic Polyangiitis (현미경적 다발혈관염을 동반한 폐섬유증 1예)

  • Jeong, Jae-Ho;Kang, Sung-Hee;Park, Se-Jung;Kim, Dal-Yong;Kim, Woo-Sung;Kim, Dong-Soon;Song, Jin-Woo
    • Tuberculosis and Respiratory Diseases
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    • v.70 no.3
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    • pp.257-260
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    • 2011
  • A 65-year-old woman was admitted due to poor oral intake and a dry cough over the previous 3 months. The physical examination was remarkable for bibasilar crackles, and plain chest radiography showed reticulation in both lower lung fields. A pulmonary function test demonstrated a restrictive pattern with a reduced diffusing capacity of the lung for carbon monoxide. High resolution computed tomography showed reticulation and honey-combing in both peripheral lung zones, which was consistent with usual interstitial pneumonia pattern. Her skin showed livedo reticularis. The erythrocyte sedimentation rate and C-reactive protein level were elevated, and hematuria was noted on urinary analysis. A serologic test for auto-antibodies showed seropositivity for Myeloperoxidase-Anti-neutrophil cytoplasmic antibody (MPO-ANCA). A kidney biopsy was performed and showed focal segmental glomerulosclerosis. She was diagnosed as having pulmonary fibrosis with microscopic polyangiitis (MPA) and treated with high dose steroids. Here we report a case of pulmonary fibrosis coexistent with microscopic polyangiitis.

Feasibility of Endoscopic Endonasal Approach for Recurrent Pituitary Adenomas after Microscopic Trans-Sphenoidal Approach

  • Hwang, Joo Min;Kim, Yong Hwy;Kim, Jin Wook;Kim, Dong Gyu;Jung, Hee-Won;Chung, Young Seob
    • Journal of Korean Neurosurgical Society
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    • v.54 no.4
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    • pp.317-322
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    • 2013
  • Objective : The surgical approach for recurrent pituitary adenoma after trans-sphenoidal approach (TSA) is challenging. We report the outcomes of the endoscopic TSA for recurrent pituitary adenoma after microscopic TSA. Methods : From February 2010 to February 2013, endoscopic TSA was performed for removal of 30 recurrent pituitary adenomas after microscopic TSA. Twenty-seven (90%) patients had a clinically non-functioning pituitary adenoma. Twenty-four (80%) patients suffered from a visual disturbance related to tumor growth. The clinical features and surgical outcomes were retrospectively analyzed for the ophthalmological, endocrinological, and oncological aspects. Results : The mean tumor volume was 11.7 $cm^3$, and gross total resection was achieved in 50% of patients. The volumetric analysis based on the postoperative MR showed that the mean extent of resection rates were 90%. Vision was improved in 19 (79%) of 24 patients with visual symptoms, and endocrinological cure was achieved in all of three functioning pituitary adenomas; however, the post-operative follow-up endocrinological examination revealed a new endocrinological deficit in one patient. Two patients required antibiotics management for post-operative meningitis. Conclusion : The endoscopic TSA can be an effective treatment option for recurrent pituitary adenoma after microscopic TSA with acceptable outcome.

Studies on Dermatophytosis of Common Seal and Elephant (물범 및 코끼리의 백선균증(白癬菌症)에 관한 연구(硏究))

  • Choi, Won Pil
    • Korean Journal of Veterinary Research
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    • v.21 no.2
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    • pp.113-116
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    • 1981
  • This experiment was undertaken to determine the causative agent of dermatophytosis occured in the 3 common seals and a elephant which were derived from the Tae-gu and Busan zoological gardens. Direct microscopic examination, culture and pathogenicity test were performed for the samples obtained from the skin lesions of the affected common seals and elephant. The causative agent was identified as Trichophyton mentagrophytes exclusively in these cases, and the present report describes the first cases of the common beat and elephant ringworm.

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Calculation of Differential Reflection Coefficient for Isolated Microscopic Well Structure

  • Lee, Jong-Tai
    • ETRI Journal
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    • v.21 no.3
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    • pp.41-48
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    • 1999
  • We have calculated differential reflection coefficient for isolated well structure of micro-scale, etched on dielectric surface. The differential reflection coefficient is computed using Green's second integral theorem. The purpose of our computation is to find a class of well profiles which give maximal diffusive scattering. To have such a maximal effect, we have concluded that the waist radius of Gaussian beam and its wavelength should be comparable to the well width and that well depth has to be larger than a wavelength. Exact calculation of differential reflection coefficients of dielectric surface with isolated structure on it may be used for the examination of dielectric surfaces and also in making simple but efficient diffuser.

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