• Animal Systematics, Evolution and Diversity
• /
• v.38 no.1
• /
• pp.14-19
• /
• 2022

Leptochiton Gray, 1847 is one of the most ancient chiton groups which includes more than 130 species that occur in cold and deep waters worldwide. Due to their small-sized body, they are often confused as juveniles of other chiton species. Moreover, lack of morphological information makes species identification of this group very challenging. To date, only two Leptochiton species(L. fuliginatus and L. rugatus) have been reported from Korean waters. In this study, we found L. hakodatensis(Thiele, 1909) for the first time in Korea and described microscopic morphological characters of valves (tegmentum sculpture), girdle scale, and radula using a scanning electron microscopy (SEM). Leptochiton hakodatensis is morphologically similar to L. fuliginatus and L. rugatus, but differently characterized by having dorso-ventrally rounded (not carinated) intermediate valves, girdle (perinotum) scales sculptured with 4-7 longitudinal ribs, and bicuspid major lateral teeth of radula. In addition to morphological examination, we determined the partial mitochondrial cytochrome c oxidase subunit I(cox1) as a DNA barcode sequence information. This is the first report that describes microscopic characters (tegmentum of valves, girdle structure, and radula) of L. hakodatensis using a SEM. This study provides a morphological basis for describing Leptochiton species and discovery of a "hidden" species of this genus.

• Korean Journal of Plant Resources
• /
• v.8 no.3
• /
• pp.303-318
• /
• 1995

Microscopic structures of epidermis and palynology and isozyme analysis were examined to find out the intergeneric and interspecific relationships and consequently to confirm the position of Hanabusaya aiatica as an endemic genus among 4 genera and 9 species belonging to the Hanabusaya asiatica, Korean endemic, and its allied groups. In the examination of microscopic structures of epidermis, cell patterns of perianth and ultrastructure of seed coat were found to be useful characters for the identification of the 4 genera and 9 species. Palynological characters such as microscopic structure and overall morphology of pollen grains were, however, not enough to distinguish them because of the great variabilities in these traits. Isozyme analysis showed that H. asiatica was very closly related to Campanula punctata and C. takesimana, though there were variations among populations and collected areas in some classified groups, depending on classified groups. Based on these results, the position of H. asiatica as an endemic genus was well confirmed.

• Mycobiology
• /
• v.29 no.4
• /
• pp.194-197
• /
• 2001

Schizopora paradoxa is a white rot fungus showing great variation in hymenophoral configuration. It has been often treated as close to Irpex or Hyphodontia and frequently confused with S. flavipora which was identified as an unrecorded species in Korea. Distinct features including sizes of basidia and basidiospores as well as hymenophoral shape make S. paradoxa differ from S. flavipora. Remarkable characters of hymenophores and microscopic structures are described and their significance is discussed for the taxonomy of Korean Schizopora.

• The Korean Journal of Mycology
• /
• v.26 no.3 s.86
• /
• pp.407-410
• /
• 1998

The aphid-attacking fungus Pandora neoaphidis was collected during May and June, 1998 at various localities: two localities at Kyunggi, three at Kangwon, one at Kyungbuk and one at Chungnam Provinces. Occurrence of this fungus has never been recorded in Korea. The potential host aphids are Acyrthosiphon kondoi, Myzus persicae, Lipaphis erysimi, Sitobion avenae, Aphis rumicis, Dactynotus species and unknown species. Microscopic characters of the fungus are described and illustrated. Pandora neoaphidis studied in this study has an extremely broad range in conidial size and shape even within a single specimen. Such variable size and shape are typical characters of the fungus. During microscopic examination of specimens, the resting-spore phase was not observed.

• Mycobiology
• /
• v.30 no.3
• /
• pp.133-138
• /
• 2002

Through seven field surveys to the Kyeryoungsan National Park from July to December of the year 2001, 116 specimens of the Aphyllophorales were collected and taxonomically examined. All the specimens were identified to the species by observation of morphological and microscopic characters, along with old specimens from the Kyeryoungsan National Park of SFC herbarium. They amounted to 10 families, 37 genera and 52 species, and two species among them, Antrodia xantha and Ceriporia viridans, were confirmed as new to Korea and are described here as unrecorded species.

• The Korean Journal of Mycology
• /
• v.26 no.2 s.85
• /
• pp.200-210
• /
• 1998

To study the species diversity of the genera Strobilomyces and Heimiella in Korea, 52 specimens were collected from 1982 to 1996 at 25 locations including Odaesan. Collected specimens were observed and three species among them were identified and described by observing macroscopic and microscopic characters. The three species are as follows; Strobilomyces floccopus (Vahl ex Fries) Karsten, S. confusus Singer, Heimiella japonica Hongo.

• The Plant Pathology Journal
• /
• v.24 no.1
• /
• pp.84-86
• /
• 2008

Field surveys and specimen collections of the rust fungal pathogen Gymnosporangium were carried out for 15 years from 1985 through 1999 in various locations of Korea. Macroscopic and microscopic examinations of morphological characteristics of aecia from the collected specimens revealed that Gymnosporangium globosum is the causal agent of American hawthorn rust disease on Crataegus pinnatifida and C. pinnatifida varmajor. The host plants are new for this rust fungus. G. globosum was found only in Gyeonggi and Chungbuk provinces, indicating that its distribution in Korea is limited. This is a first full description on morphological characters of aecia of G. globosum in Korea.

• Herbal Formula Science
• /
• v.12 no.1
• /
• pp.177-193
• /
• 2004

Objectives: This study has been done to compare the Haematite's composition before and after using processing method and to clarify its quality and naming through the historical herbal books. Methods: I studied the Haematite and processed Haematite using physical and chemical methods which contain microscopic analysis, X-ray diffraction analysis, thermal analysis, XRF, ICP, etc, to compare the compositions and its characters. Results: Haematite is a metallic oxide(Hematite, $Fe_2O_3$). Haematite contains a main ingredients of crude Haematite Fe 40.336% - 62.174%, processed Haematite $50.499%{\sim}62.791%$(a theory value is 69.944% the korean pharmacopoeia wants >45%) and small ingredients, also. Conclusion: A radio-element contents of U, Th contain less than average contents of the lithosphere's U 1.8ppm, Th 7.2ppm.

• Journal of the Korean Wood Science and Technology
• /
• v.33 no.2 s.130
• /
• pp.1-7
• /
• 2005

Tree species of wooden structural members of the Beomeo Temple were identified based on light and scanning electron microscopic characters in the present study. Of 10 structural members, 9 softwoods and 1 hardwood were identified. Among softwood members, 7 belonged to hard pine of the Sylvestris section, and the remaining 2 to hard pine of other than the Sylvestris section and hemlock of the genus Tsuga, respectively. A single hardwood member was identified as white oak of the Prinus section under the subgenus Lepidobalanus.

• Applied Microscopy
• /
• v.39 no.1
• /
• pp.65-72
• /
• 2009

Cryo-SEM which enables specimens to be observed in frozen form has been used to study liquid specimens in their native states. Cryo-methods, sample preparation for cryo-SEM, are quite complex and involve several discrete but vitally interconnected steps which are rapid cooling, fracturing, sectioning, etching and coating. It is important to select practical techniques and to optimize conditions of each steps considering analytic purpose and specimen characters, viz., sample dimension, water contents. In this study, etching methods and sample preparation before freezing had been studied for observation of cyanobacteria, Synechocystis sp. PCC 6803 using cryo-SEM and their cryo-SEM images were compared to Conventional SEM (CSEM) images treated by chemical fixation. We could observe the improved morphological images of the pili of the surface and membranes of Synechocystis sp. PCC 6803 and the three-dimensional architectures of their biofilm, which were difficult to observe using chemical fixation and CSEM. These results suggest that cryo-methods/cryo-SEM are useful techniques for morphological study of biological specimen.