Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
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Description of Microscopic Morphology of Leptochiton hakodatensis (Mollusca: Polyplacophora)

  • Received : 2021.11.15
  • Accepted : 2021.11.17
  • Published : 2022.01.31
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Abstract

Leptochiton Gray, 1847 is one of the most ancient chiton groups which includes more than 130 species that occur in cold and deep waters worldwide. Due to their small-sized body, they are often confused as juveniles of other chiton species. Moreover, lack of morphological information makes species identification of this group very challenging. To date, only two Leptochiton species(L. fuliginatus and L. rugatus) have been reported from Korean waters. In this study, we found L. hakodatensis(Thiele, 1909) for the first time in Korea and described microscopic morphological characters of valves (tegmentum sculpture), girdle scale, and radula using a scanning electron microscopy (SEM). Leptochiton hakodatensis is morphologically similar to L. fuliginatus and L. rugatus, but differently characterized by having dorso-ventrally rounded (not carinated) intermediate valves, girdle (perinotum) scales sculptured with 4-7 longitudinal ribs, and bicuspid major lateral teeth of radula. In addition to morphological examination, we determined the partial mitochondrial cytochrome c oxidase subunit I(cox1) as a DNA barcode sequence information. This is the first report that describes microscopic characters (tegmentum of valves, girdle structure, and radula) of L. hakodatensis using a SEM. This study provides a morphological basis for describing Leptochiton species and discovery of a "hidden" species of this genus.

Keywords

Acknowledgement

This work was supported by National Marine Biodiversity Institute of Korea (2021M01100) and a grant from the National Institute of Biological Resources(NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR 202130202).

References

  1. Carpenter PP, 1864. Supplementary report on the present state of our knowledge with regard to the Mollusca of the west coast of North America. Report of the British Association for the Advancement of Science, 1863:517-686.
  2. Dall WH, 1879. Report on the limpets and chitons of the Alaskan and Arctic regions, with descriptions of genera and species believed to be new. Proceedings of the United States National Museum, 1:281-344. https://doi.org/10.5479/si.00963801.1-48.281
  3. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3:294-299.
  4. Gould AA, 1859. Description of shells collected by the North Pacific Exploring Expedition. Proceedings of the Boston Society of Natural History, 7:161-165. https://doi.org/10.5962/bhl.part.4821
  5. Gould AA, 1862. Otia Conchologica: Descriptions of shells and mollusks, from 1839 to 1862. Gould and Lincoln, Boston, pp. 1-256.
  6. Jakovleva AM, 1952. Shell-bearing mollusks (Loricata) of the seas of the USSR. Fauna USSR, 45:1-107.
  7. Kaas P, Van Belle RA, 1985. Monograph of living chitons(Mollusca: Polyplacophora). Vol. 1. Order Neoloricata: Lepidopleurina. Brill EJ/Baclhuys W, Leiden, pp. 1-244.
  8. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A, 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28:1647-1649. https://doi.org/10.1093/bioinformatics/bts199
  9. Kiel S, Little CTS, 2006. Cold-seep mollusks are older than the general marine mollusk fauna. Science, 313:1429-1431. https://doi.org/10.1126/science.1126286
  10. Okutani T, 2000. Marine mollusks in Japan. Tokai University Press, Tokyo, pp. 1-1173.
  11. Pilsbry HA, 1892. Monograph of the Polyplacophora. In: Manual of conchology (Ed., Tryon GW). Vol. 14. Academy of Natural Sciences, Philadelphia, pp. 1-128.
  12. Sigwart JD, 2008. Gross anatomy and positional homology of gills, gonopores, and nephridiopores in "basal" living chitons (Polyplacophora: Lepidopleurina). American Malacological Bulletin, 25:43-49. https://doi.org/10.4003/0740-2783-25.1.43
  13. Sirenko BI, 1976. Chitons from the Vostok Bay (Sea of Japan). In: Biological investigations of Vostok Bay (Ed., Kasjanov VL). Institute of Marine Biology, Far Eastern Scientific Center, Vladivostok, pp. 87-91.
  14. Sirenko BI, 2013. Four new species and one new genus of Jurassic chitons(Mollusca: Polyplacophora: Lepidopleurida) from the Middle Russian Sea. Proceedings of the Zooloical Insitute RAS, 317:30-44. https://doi.org/10.31610/trudyzin/2013.317.1.30
  15. Sirenko BI, 2015a. Shallow and deep-sea chitons of the genus Leptochiton Gray, 1847 (Mollusca: Polyplacophora: Lepidopleurida) from Peruvian and Chilean waters. Zootaxa, 4033:151-202. https://doi.org/10.11646/zootaxa.4033.2.1
  16. Sirenko BI, 2015b. Leptochiton antarcticus (Mollusca, Polyplacophora): a new species from the Southern Ocean. Ruthenica, 25:139-146.
  17. Sirenko BI, Sigwart JD, 2021. Leptochiton subrugatus sp. nov. (Mollusca: Polyplacophora) from low boreal waters of northern Pacific. Marine Biodiversity, 51:1-10. https://doi.org/10.1007/s12526-021-01190-z
  18. Taki I, 1938. Report of the biological survey of Mutsu Bay 31. Studies on chitons of Mutsu Bay with general discussion on chitons of Japan. The Science Reports of the Tohoku Imperial University, 4:323-423.
  19. Taki I, 1962. A list of the Polyplacophora from Japanese Islands and vicinity. Venus, 22:29-53.
  20. Taki I, 1964. Classification of the class Polyplacophora, with a list of Japanese chitons. Venus, 22:401-414.
  21. Taki I, Taki I, 1929. Studies on Japanese chitons (2). Venus, 1:141-153.
  22. Thiele J, 1909. Revision des Systems der Chitonen. Teil I. Zoologica Stuttg, 22:1-70.
  23. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876