• Title/Summary/Keyword: microscope observation

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Characteristics of attach of biomass on PE substratum under anaerobic condition (폴리에틸렌 담체에 부착된 혐기성 생물막 부착 특성)

  • 이승란;김도한;나영수;이창한;박영식;윤태경;송승구
    • Journal of Environmental Science International
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    • v.11 no.4
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    • pp.327-332
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    • 2002
  • Optical microscope, SEM (Scanning Electron Microscopy) and fluorescent microscope were used for qualitative and morphological studies of the attached biomass on PE (polyethylene) substratum under anaerobic condition. It was shown by the observation of optical microscope that the initial attachment of biomass began in crevices of the surface of PE. The shape and structure of the attached biofilm could be observed by SEM photographs, but species of bacteria were and methanogens were not classified. A large number of methanogenic bacteria were identified on the surface of PE substratum by fluorescence under 480nm of radiation. It was estimated that methanogenic bacteria was also related to initial attachment of biomass under anaerobic condition.

Nano-Scale Surface Observation of Cyclically Deformed Copper and Cu-Al Single Crystals (반복변형된 Cu 및 Cu-Al 단결정 표면형상의 나노-스케일 관찰)

  • 최성종;이권용
    • Tribology and Lubricants
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    • v.16 no.5
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    • pp.389-394
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    • 2000
  • Scanning Probe Microscope (SPM) such as Scanning Tunneling Microscope (STM) and Atomic Force Microscope (AEM) was shown to be the powerful tool for nano-scale characterization of material surfaces. Using this technique, surface morphology of the cyclically deformed Cu or Cu-Al single crystal was observed. The surface became proportionately rough as the number of cycles increased, but after some number of cycles no further change was observed. Slip steps with the heights of 100 to 200 nm and the widths of 1000 to 2000 nm were prevailing at the stage. The slipped distance of one slip system at the surface was not uniform, and formation of the extrusions or intrusions was assumed to occur such place. By comparing the morphological change caused by crystallographic orientation, strain amplitude, number of cycles or stacking fault energy, some interesting results which help to clarify the basic mechanism of fatigue damage were obtained. Furthermore, applicability of the scanning tunneling microscopy to fatigue damage is discussed.

Isolation of Ectroparasitic Bdellovibrio sp. from Several Soils (토괴중에서의 bdellovibrio sp.의 분리시도)

  • 고춘명;이봉기;등영건
    • Korean Journal of Microbiology
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    • v.15 no.1
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    • pp.42-45
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    • 1977
  • Bdellovibrio sp. is an ectoparasitic bacteria which is predatory and parasitic upon other bacteria. This study was carried out the isolation of Bdellovibrio sp. from several soil smaples and observation of this organisms by means of electron microscope. The results are as follows ; The primary isolated Bdellovibrio sp. from soil is an obligate intracellular rod form parasite and possess a monoflagella.

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The Observation of Fatigue Striations for Aluminum Alloy by Atomic Force Microscope(AFM) (원자력 현미경(AFM)에 의한 알루미늄 합금의 피로 스트라이에이션 관찰)

  • Choe, Seong-Jong;Gwon, Jae-Do
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.24 no.4 s.175
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    • pp.955-962
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    • 2000
  • Scanning Probe Microscope (SPM) such as Scanning Tunneling Microscope (STM) and Atomic Force Microscope (AFM) was shown to be the powerful tool for nano-scale characterization of a fracture surface . AFM was used to study cross sectional profiles and dimensions of fatigue striations in 2017-T351 aluminum alloy. Their widths (SW) and heights (SH) were measured from the cross sectional profiles of three-dimension AFM images. The following results that will be helpful to understand the fatigue crack growth mechanism were obtained. (1) Coincidence of the crack growth rate with the striation width was found down to the growth rate of 10-5 mm/cycle. (2) The relation of SH=0.085(SW)1.2 was obtained. (3) The ratio of the striation height to its width SH/SW did not depend on the stress intensity factor range K and the stress ratio R. (4) Not only the SW but also the SH changed linearly with the crack tip opening displacement (CTOD) when plotted in log-log scale. From these results, the applicability of the AFM to nano-fractography is discussed.

Morphological Study of the Digestive Tract of the Mud Crab (Hemigrapsus Penicillatus De Haan) and the Symbiotic Crab (Pinnotheres cyclinus Shen)

  • Moon, Young-Wha;Kim, Han-Hwa
    • Animal cells and systems
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    • v.3 no.4
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    • pp.407-412
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    • 1999
  • The influence of eating habits and food type on the ultrastructural characteristics of the digestive tracts was studied under the scanning and transmission electron microscopes in two crustacean decapods (Hemigrapsus penicillatus De Haan; mud crab, Pinnotheres cyclinus Shen; symbiotic crab). The relative ratio of the length of midgut versus hindgut was 1:1 in the mud crab, but 4:1 in the symbiotic crab. Observation through the scanning electron microscope revealed that the midguts of both species have densely-arranged longitudinal mucosal folds with a smooth surface. In the hindgut of the mud crab, mucosal folds were longitudinally oriented, clusters of two to five spines were observed on the cuticular surface, and the length of the spine in the distal hindgut was longer than that in the proximal portion. In the symbiotic crab, the mucosal folds were irregulary arranged, and numerous rudimentary spinal structures were noted on the cuticular surface. Through observation of a transmission electron microscope, the epithelial cells of the midgut in both species had numerous microvilli, but the length of the microvilli was slightly longer in the mud crab than in the symbiotic crab. The central layer of the basement membrane and the muscular layer of the midgut were more developed in the mud crab than in the symbiotic crab. The thickness of the cuticular layer over the hindgut surface in the mud crab was about 4 times than that of the symbiotic crab.

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State-selective Dissociation of Water Molecules on MgO Films Using LT-STM

  • Shin, Hyung-Joon;Jung, J.;Motobayashi, K.;Kim, Y.;Kawai, M.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.112-112
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    • 2011
  • The interaction of water molecules with solid surfaces has been a subject of considerable interests, due to its importance in the fields from atmospheric and environmental phenomena to biology, catalysis and electrochemistry [1,2]. Among various kinds of surfaces, a lot of theoretical and experimental studies have been performed regarding water on MgO(100), however, to date, there has been no direct observation of water molecules on MgO by scanning tunneling microscope (STM) as compared with those on metal surface. Here, we will present the direct observation and manipulation of single water molecules on ultrathin MgO(100) films using low-temperature scanning tunneling microscope (LT-STM) [3]. Our results rationalize the previous theoretical predictions of isolated water molecules on MgO including the optimum adsorption sites and non-dissociative adsorption of water. Moreover, we were able to dissociate a water molecule by exciting the vibrational mode of water, which is unattainable on metal surfaces. The enhanced residual time of tunneling electrons in molecules on the insulating film is responsible for this unique pathway toward dissociation of water.

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Chemical Methods Used in Petrological Analysis of Koongarra Uranium Ore Samples in ASSAR Natural Analogue Program

  • Park, Yong-Joon;Pyo, Hyung-Ryul;Kim, Ji-Young;Kim, Won-Ho
    • Nuclear Engineering and Technology
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    • v.30 no.6
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    • pp.518-530
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    • 1998
  • A natural analogue study has been performed for the Koongarra uranium ore deposit in Australia as an international agreement of the Analogue Studies in the Alligator Rivers Region (ASARR). Rocks obtained from the Koongarra deposit, Northern Territory of Australia, were examined in order to understand uranium migration processes of primary and secondary ore-body in both weathered and unweathered zones. Total alpha activities of rock samples were measured to compare the relative amount of uranium in the sample. Uranium distributions have been investigated by means of both the alpha-autoradiography and the fission track registration technique after irradiation in a flux of thermal neutrons (~10$\times$$10^{13}$nㆍ$cm^{-2}$ㆍs$^{-1}$) for 2 minutes. The mineral phases corresponding to the registered alpha-tracks and fission tracks were identified by petrological observation with optical microscope as well as X-ray diffraction and electron microprobe analysis (EPMA). Uranium was found mostly inside of the fracture of the quartzite and its mineral phase was identified as sklodowskite. The mineral phase associated with high uranium concentration was found as illeminite by petrological observation with optical microscope as well as EPMA.

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Failure Analysis of Carburized Gears by Microstructural Observation (침탄처리된 기어의 미세 조직학적 손상 원인분석)

  • Chun, Hae Dong;Chang, Sung Ho;Kim, Kyung Wook;Kuk, Youn Ho
    • Journal of the Korean Society for Heat Treatment
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    • v.27 no.4
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    • pp.191-201
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    • 2014
  • The gear was made of SNC815 case-carburized, quench hardened and tempered steel. The gears were failed far earlier than the expected service life used in the industrial site. Causes of the failed gear were analyzed by microstructure observation. By the SEM and micro structure examinations, the damaged surfaces had been weared and failed by fatigue. Through microscope observation on the damaged surface, it was found that the cause of failure was determined by external overloading and the initial stage of the damage was closely related to complex contact fatigue failure. The overload and contact fatigue contributed to the early failure cause.

Protection Effect of Natual Matter and Radiation Damage on Kidney Tissue (신장 조직의 방사선 손상과 천연물질의 방어기전 연구)

  • Ji, Tae-Jeong
    • The Journal of the Korea Contents Association
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    • v.9 no.12
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    • pp.376-384
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    • 2009
  • This research has microstructure observation to find tissue damage mechanism and radio-protection effect on mouse kidney tissue. The result observation of a Light Microscope(LM); The kidney tissue after 5Gy irradiation observed a glomerulus atrophy, also crack distance to base membrane of a convoluted tubules. The kidney tissue after 10Gy irradiation observed out flow cytoplasm to membrane break of a convoluted tubules. The result observation of a Transmission Electron Microscope(TEM); The kidney tissue of after 5Gy irradiation has to breaking a inside cristae and membrane of mitochondria, also show definite damage of nucleus membrane. 10Gy irradiation has all the more damage a base membrane and thickness of lysosome. However, Propolis eating groups observed normal to nucleus membrane and small body of intracellular. therefore We considered "Propolis" as make radio protection function to kidney tissue of the greater part.