• 한국미생물·생명공학회지
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• 제30권2호
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• pp.184-188
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• 2002

Lactobacillus sake는 냉장온도(2-4 ℃)이하에서도 성장이 가능한 통성혐기성균으로서, sausage와 진공포장육의 제조에 관여하며, 동양에서는 청주의 발효와 김치의 숙성에도 큰 비중을 차지하는 것으로 알려지고 있다. 그러나 L. sake는 이들 식품의 제조시 과도한 발효의 진행으로 식품을 변질시켜 가치를 저하시키기도 한다. 본 연구에서는 L. sake의 생육을 효과적으로 저지하는 박테리오신을 생산하는 미생물을 김치로부터 분리하여 Lactobacillus sp. Oh-B3로 명명하였다. 최대의 박테리오신을 생산하기 위한 분리균의 배양조건은 초기 pH를 8.0으로 조절한 MRS배지에 접종하여 25℃에서 24시간동안 배양했을 때 최대의 박테리오신을 생산하였다. 분리균의 배양액을 원심분리하여 균체를 제거한 상등액에 ammonium sulfate를 첨가한 뒤, 얻어진 침전물을 투석하고 여과한 뒤, -20℃에 보관하며 조박테리오신 용액으로 사용하였다. 분리균이 생산하는 박테리오신은 pH 2.0-9.0의 넓은 범위에서 비교적 안정한 활성을 나타내었으며, 그람음성균에 대해서는 항균활성을 보이지 않았고 일부 그람양성균에 대해서만 활성을 나타내었다. 조박테리오신 용액에 각각의 단백질 분해효소를 처리한 후 박테리오신의 잔존활성을 측정한 결과 분리균이 생산하는 박테리오신은 대부분의 단백질분해효소에 의해 활성이 제거되었다. 그리고 조박테리오신 용액을 열처리를 한 뒤 잔존활성을 측정한 결과 분리균이 생산하는 박테리오신은 100℃에서도 매우 안정하였으며, autoclave를 하여도 50％ 이상의 활성이 유지되었다. 일반적인 박테리오신과 마찬가지로 분리균이 생산하는 박테리오신은 bacteriocidal action을 하는 것으로 관찰되었다.

• 한국환경과학회지
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• 제25권3호
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• pp.439-446
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• 2016

Domestic sewerage treatment plant is operated by activated sludge method and its modified method by using microorganism. In most cases, a method of using microorganism is directly controlled by the operator based on individual judgment through factors of DO, pH and ORP. In addition, under aerobic condition in bioreactor, energy consumption including excessive air injection is learned to be somewhat plenty. In order to solve this problem, in most of the process, improvement of internal recycling and activated environmental factor of microorganism were researched extensively. However, as factors are changed depending on various conditions, it is not sufficient as an indicator of judgment. As such, a research on operation of bioreactor that measures metabolic change in short time by directly measuring activated condition of microorganism using NADH fluorometer is required in reality.It is considered that the method like this could supplement problem of energy consumption being occurred in the existing treatment method and operational optimization of bioreactor would be enabled by controlling optimal air volume. Therefore, in this study, in order to obtain optimal operational indicator of bioreactor, proper air volume control test was performed and through batch test and site evaluation, possibility of NADH sensor being utilized as operational control indicator of bioreactor is intended to be analyzed. In order to compare with measured value, DO, ORP that are operational control indicator of existing bioreactor were used. As MLSS concentration was increased through batch test, NADH value was increased and site evaluation also showed similar tendency to batch test. Resultantly, it could be confirmed that changing level of NADH fluorometer was a sensor that could measure bioreactor condition effectively and optimized scale of bioreactor is considered to be utilized.

• 생명과학회지
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• 제12권5호
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• pp.523-527
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• 2002

본 연구에서는 발효에 따른 돌산 갓김치 즙액내의 미생물량의 항산화 효과와의 관계, 발효 중 sinigrin 함량 변화와 일반성분의 변화를 조사하였다. 돌산 갓김치는 돌산 갓, 파, 마늘, 생강 그리고 고추가루를 첨가하여 제조하였으며, 2$0^{\circ}C$, 50일간 발효하였다. 갓김치 즙액내의 미생물의 수는 적숙기인 10일째까지 상승하다가 감소하는 경향이었는데 항산화 효과도 비슷한 앙상이어서 갓김치 발효 중 미생물에 의한 대사산물이 항산화 효과 증가와 같은 생리기능성에 큰 영향을 주었으리라 사료되며, sinigrin 함량도 발효가 진행되면서 적숙기에 최대로 나타나서 기능성 물질중 하나로 사료된다. 또한 일반성분 변화 중 조단백질의 양이 후숙기로 갈수록 증가하는 경향이었는데, 이는 각종 미생물의 증가로 인해 증가한 것으로 사료된다.

• 한국물환경학회지
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• 제21권2호
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• pp.118-124
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• 2005

The oxygen solubilized device(O.S.D) and standardized microorganism culture system is more efficient than physical and chemical purification techniques in closed water. This study was to determine how the O.S.D and standardized culture system is efficient in purification capacity in closed water based on the lab scale and pilot plant. In the batch test, inducing the quantitative results from pilot plant operation condition, removal efficiency of COD and TN were about 48.3% and 35% respectively, while SS and chlorophyll-a were 94.9% and 68.7%. The pilot plant results showed that suspended solid(SS) and chlorophyll-a removal efficiency were 60% and 59% respectively, due to coagulation characteristics by standardized culture. Total nitrogen(TN) and total phosphorus(TP)showed good effect for the purification of target pond water quality from field data. Additionally, released velocity was determined in control condition of $5.31mgPO{_4}^{3-}{\cdot}m^{-2}{\cdot}day^{-1}$ and $2486.8mgCOD{\cdot}m^{-2}{\cdot}day^{-1}$. Otherwise, phosphate and COD reflux in the aeration and microorganism condition was showed $-9.95mgPO{_4}^{3-}{\cdot}m^{-2}{\cdot}day^{-1}$ and $-397.88mgCOD{\cdot}m^{-2}{\cdot}day^{-1}$. This technology is the most effective not only removal of SS and chlorophyll-a but also control of phosphate and COD release which is very important phenomena in evaluating water quality in closed water like a reservoir and pond.

• 한국염색가공학회지
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• 제16권3호
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• pp.22-30
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• 2004

The polyester fabrics were treated with the chitosan with various solubility in optimized treatment condition. The treatment method was discussed to be a high functional finishing for the polyester fabric to obtain the high moisture absorption and anti-microorganism property by evaluating the effect of the chitosan purification method on the yield and anti-microorganism property of the chitosan. On the other hand, soluble polyurethane was added to the chitosan treatment solution and/or plasma pretreatment was done. The addition of soluble polyurethane give a high add-on ratio as well as a linen like effect of treated polyester fabric. The results were as follows: 1. In the treatment of polyester fabric by the chitosan solution, a soluble PU resin and low temperature plasma treatment were done to obtain high binding force between the fabrics and the chitosan. The add-on rate and the moisture absorption ratio of the fabrics treated with the chitosan-PU after treated with the plasma slightly increased more than those of the fabrics treated with the chitosan only. 2. Anti-static property of the fabrics treated with the chitosan decreased rapidly with increasing of the chitosan concentration. The washing fastness of the fabrics treated with the chitosan-PU after treated with the plasma was better than those of the fabrics treated with chitosan only. The wrinkle resistance of the treated fabrics decreased constantly with the concentration of the chitosan. The bending rigidity of the treated fabrics increased greatly. On the treatment of polyester fabric under optimum condition, the microorganism reduction rate kept above 90％ after 10times launderings. 3. As the polyester fabrics which has flat yam was used as a weft yams were treated with the chitosan-PU as give a functional finishing effects such as durability, moisture absorption, anti-static and anti- microorganism property. Treated polyester fabric showed a good functional finishing effect and a linen like property.

• 유기물자원화
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• 제9권3호
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• pp.88-92
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• 2001

돈분발효퇴비(P)와 혼합유박비료(B, F), 부산물퇴비(D) 4종의 유기질비료와 토양 미생물제제(M)의 시용 효과를 규명하기 위하여 배추와 상치를 공시작물로 시설하우스에서 재배하면서 생육 및 수량을 조사하였다. 유기질비료의 추천 시비량 처리구에서 수량이 가장 좋았으며, 과량 처리구에서는 비해가 발생하였으나, 미생물제제의 혼용에 의해 현저한 비해 감소 현상을 볼 수 있었다. 또한 같은 비종 및 동일한 시용량 처리구에서도 미생물제제를 혼용하였을 때 생육이 안정되었으며, 수량도 높은 경향을 보였다. 결론적으로 유기질비료의 과량처리는 비해가 발생할 수 있으며, 미생물제제의 시용으로 생육 및 수량이 향상되었다.

• 한국농공학회논문집
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• 제57권1호
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• pp.111-118
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• 2015

This research aims to utilize lapilli from the Mt. Baekdusan as environmently-friendly construction material. First of all, the neutralizing method for fabricating lapilli-cement-mixed bioblock was examined. And then, by use of the neuralized bioblock with microorganism for water purification, the growth inhibition effect against the pathogenic coliform bacillus was evaluated. The result regarding growth inhibition effect on pathogenic coliform bacillus indicates that the pretreatment condition, which is a concurrent procession with aqueous solution of 10% di-ammonium Phosphate after water curing, led to pH degraded below 10 which was the target value. Therefore it was concluded that the method was effective on bio-block neutralization. The microorganisms purifying water and di-ammonium phosphate were detected through the examination for microorganism existence on the bioblock, therefore it was concluded that the bioblock composed of lapilli and cement is able to be utilized in various structures as an environment friendly construction material.

• 한국환경보건학회지
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• 제28권1호
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• pp.85-92
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• 2002

A measurement of indoor microorganism using Reuter Centrifugal Air Sampler(RCS) was undertaken during October 1991 - February 1999 and 6-Stage Cascade Air Sampler was undertaken during May 2001 - June 2001 in Seoul. Sites including book store, department store, theater, subway station, underground shopping center, hospital, office building, sports facility, and eduationa institutio were chosen to measure indoor microorganism. The results were as follows: 1. The average of total microorganism collected on the agar strip GK-A media were, in the order, subway station, hospital, underground shopping center, department store, book store, theater, sports facility, educational institution, office building in sites. The highest concentration of 711cfu/m$^3$ was found in the subway station and the lowest concentration of 44cfu/m$^3$ was found in office building. 2. The average of staphylococci collected on the agar strip GK-S media, in the order, were subway station, underground shopping center, hospital and department store, department store, theater, office building, sports facility and educational institution in sites. The highest concentration of 502cfu/m$^3$ was found in the subway station and the lowest concentration of 14cfu/m$^3$ was found in sports facility and educational institution. 3. The average of fungus collected on the agar strip GK-HS media, in the order, were underground hospital, shopping center, theater, subway station, department store, book store, sports facility, educational institution, and office building in sites. The highest concentration of 252cfu/m$^3$ was found in the hospital and the lowest concentration of 32cfu/m$^3$ was found in office building. 4. Ratio of Indoor/Outdoor, determined by site was 1.12-2.38 in total count, 1.00-2.35 in staphylococci, and 0.99-1.34 in fungus. 5. The positive results of test were 12-24% in indoor and 9-43% in outdoor. 6. By gram staining gram positive cocci were 59.9%, gram positive bacill 24.4%, gram negative bacilli 10.4%, and gram negative cocci 0.5%.

• 한국식품영양과학회지
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• 제35권10호
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• pp.1456-1460
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• 2006

변질된 팥앙금에서 분리한 균주는 광학 및 전자현미경을 통하여 관찰한 결과 그람양성 간균으로 포자를 형성하고 형성된 집락은 원형으로 점질성의 물질을 생산하는 mucoid type이었으며, 표면은 smooth 하였고 광택을 나타내었다. 미생물 동정 시스템($MicroLog^{TM}$ System, Release 4)인 VITEK 2 COMPACT을 이용하여 분리된 균주의 46개의 생화학적 분석을 비교한 결과 팥앙금의 부패 원인균은 Bacillus subtilus로 동정 (97.0%)되었으며, 보다 더 정확한 동정을 위하여 부패균의 세포벽 지방산 조성을 분석하여 Midi sherlock시스템의 databank와 비교 분석시 그 유사성이 0.724로 분석되었다. Bacillus subtilus의 포자의 D-value는 $115^{\circ}C$에서의 4.85분이 었고, $121^{\circ}C$에서는 0.69분, $125^{\circ}C$에서는 0.48분이었으며, Z-value는 $9.71^{\circ}C$이었다. $45^{\circ}C$에서 수분활성도에 따른 분리균의 생육 상태를 관찰한 결과 수분활성도 0.92이하에서는 균이 증식되지 않았고, 0.94에서는 증식되는 것으로 관찰되었다.

• 한국농공학회논문집
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• 제53권2호
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• pp.75-82
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• 2011

This study was performed to evaluate the water purification properties of bio-composites planting blocks using oyster shell and effective microorganism that have high absorption ability of heavy metals and organics to develop environmentally friendly river embankment technique contained various factors such as oyster shells, effective microorganism, porous concrete and planting embankment block. To maximize greening effect, the seeds were arbitrarily sown. In addition, in order to analyze the effect of water quality purification after the planting, the samples were collected from each designated zone 1, 7 and 30 days after steeping in water. Then, the samples were analyzed in terms of seven test items such as SS, BOD, COD, T-N, T-P, pH, etc. on the basis of the test method for water pollution. The following conclusions were reached from the test result. As a result of analysis for water quality purification for the concrete block containing the effective microorganism, it was found that the values for SS, BOD, T-N and T-P for the sample taken after 30 days were lower than the initial values, which indicated that the water purification effect had been created. The result of the water quality purification analysis for the concrete block containing oyster shell showed that the values for SS, BOD, COD and T-P for the sample taken after 30 days were lower than the initial values which also indicated that it had been effective in water quality purification.