• 제목/요약/키워드: microorganism growth

검색결과 579건 처리시간 0.022초

카네이션의 시설재배에서 길항성 세균을 이용한 Fusarium Wilt 의 생물학적 방제 (Biological Control of Fusarium Wilt of Carnation Plants by Antagonistic Microorganism in Greenhouse)

  • 조정일;조자용
    • 한국유기농업학회지
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    • 제12권2호
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    • pp.183-196
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    • 2004
  • This study was carried out to screen and select the effects of antifungal bacterial strains which inhibit the growth of plant pathogen, Fusarium oxysporum(fusarium wilt) occurred in carnation plants in greenhouse. We isolated an effective bacterial strains and investigated into the antifungal activity of the antagonistic microorganism and it's identification. Twenty bacterial strains which strongly inhibited Fusarium oxysporum were isolated from roots of carnation plants and the soil in greenhouse, and the best antifungal bacteria designated as C121, was finally selected. Antagonistic bacterial strain, C121 was identified to be the genus Bacillus sp. based on the morphological, biochemical and cultural characterizations. The Bacillus sp. C121 showed 58.1% of antifungal activity against the growth of Fusarium oxysporum. By the bacterialization of the cultural broth and the heat bacterialization culture filtrate of it, Bacillus sp. C121 was shown 92.1% and 21.0% of antifungal activity, respectively.

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온실재배 토마토에서 발생하는 위조병의 미생물학적 제어 (Biological Control of Fusarium Wilt of Tomato Plants by Antagonistic Microorganism in Greenhouse)

  • 조정일;조자용
    • 한국유기농업학회지
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    • 제11권4호
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    • pp.61-74
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    • 2003
  • This study was conducted to screen the antagonistic bacteria which inhibit the growth of plant pathogen, fusarium wilt(Fusarium oxysporum) occurred in tomato plants in greenhouse. We isolated an effective bacterial strains and investigated into the antifungal activity of the antagonistic microorganism and it’s identification. Ten bacterial strains which strongly inhibited Fusarium oxysporum were isolated from the nature, and the best antagonistic bacterial strain designated as KC175, was selected. The antagonistic strain KC175 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. The Bacillus sp. KC175 showed 58.2% of antifungal activity against the growth of Fusarium oxysporum. By the bacterialization of the culture broth and the heat bacterialization culture filtrate of it, Bacillus sp. KC175 showed 91% and 18% of antifungal activity, respectively.

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유효 미생물과 바이오 비료로 재배한 상추의 특성 (Characteristic of Lettuce (Lactuca sativa L.) Grown with Effective Microorganism and Bio-fertilizer)

  • Kim, H.S.;Choi, S.M.;Supeno, D.;A., Okka;Chung, S.W.;Kim, H.S.;Kim, J.S.;Park, J.M.;Kwon, S.H.;Kwon, S.K.;Choi, Won Sik
    • 한국산업융합학회 논문집
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    • 제17권3호
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    • pp.145-150
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    • 2014
  • The purpose of this study was to evaluate the growth of lettuce last for 36 days. Lettuce planted on the same medium with different treatment. The first treatment watering using bio-fertilizer (farmer) and second watering using effective microorganism. Lettuce grown in greenhouse with temperature $18-32^{\circ}C$.Characteristic observed that growth in height and weight of the plants. From the observations that can be generated at high observation, treatment 1 had a higher than treatment 2. On the last day treatment plant with treatment 1 had a 99.8 mm high while treatment 2 has height of 90.7 mm. Observation on severe treatment 1 has a heavier weight than treatment 2. Plants with treatment 1 have a weight on the last day of 0.15665 grams while in plants with treatment 2 weights are 0.11365 grams.

아로마 용액을 이용한 구강간호가 뇌졸중 노인의 구강상태와 구강 세균 집락에 미치는 효과 (Effects of Special Mouth Care with an Aroma Solution on Oral Status and Oral Cavity Microorganism Growth in Elderly Stroke Patients)

  • 이은혜;박효정
    • 대한간호학회지
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    • 제45권1호
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    • pp.46-53
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    • 2015
  • Purpose: This study was conducted to examine the effect of oral care with an aroma solution on oral status and oral cavity microorganism growth in elderly patients with stroke. Methods: A non-equivalent control group, with a pretest-posttest design was used in this study. The participants were assigned to the experimental group (n=30) that received oral care with an aroma solution or the control group (n=31) that received 0.9% saline solution. To identify the effect of the experimental treatments, objective/subjective assessments of oral status and oral cavity microorganism growth were performed using the oral assessment guide, oral perception guide, and oral swab culture. Data were analyzed using Chi-square test, Fisher's exact test, and t-test with the SPSS version 21.0 program. Results: The objective oral status was significantly lower in the experimental group than in the control group (t= -3.64, p<.001). There was no significant difference between the subjective oral status of the experimental group and control groups (t= -1.24, p=.109). Oral microorganism growth was significantly lower in the experimental group than in the control group (t= -7.39, p<.001). Conclusion: These findings indicate that special mouth care using an aroma solution could be an effective oral health nursing intervention for elderly patients with stroke.

숙주나물과 콩나물에 대한 부패성 미생물의 증식 양상 비교 (Comparative Study on Growth of Spoilage Microorganisms in Mungbean and Soybean Sprout)

  • 유미지;김용석;신동화
    • 한국식품위생안전성학회지
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    • 제19권1호
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    • pp.25-30
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    • 2004
  • 숙주나물과 콩나물은 blanching후 변질 속도가 크게 차이가 나는 바 이들이 Bacillus subtilis, Bacillus cereus, Pseudomonas fluorescens, Listeria monocytogenes등 4종의 부패성 및 식중독 미생물의 증식에 미치는 영향을 조사하였다. 숙주나물과 콩나물의 조리과정 중 blanching조건이 달라 각각의 조건에서 두 시료 모두 blanching하여 생균수를 측정한 결과 blanching 한 후 미생물 수는 저장시간에 따라 증가하나 그 차이가 없었다. 최소배지에 숙주나물과 콩나물 농축물 및 부패성 미생물을 접종한 후 생균수를 측정한 결과 숙주나물에 접종한 모든 균들의 초기 균수가 높았으나 24시간 이후 비슷한 증식양상을 나타내었다. 그러나 숙주나물과 콩나물의 여액을 배지로 사용하여 실험한 결과 미생물의 증식 양상은 균주 마다 다른 것으로 나타났다. 저장기간 동안 색도를 측정한 결과 숙주나물의 경우 24시간에는 색차가 거의 없다가 48시간 이후 현저한 차이를 보였고, 콩나물의 경우 24시간에 다른 계통의 색으로 변하다가 48시간 이후 현저하게 많은 차이를 보여 다른 양상을 나타내었다.

고생산성 젖산생성균 분리 및 배양 최적화 (Isolation of Microorganism with HIgh Productivity and Cultivation Optimization for Lactic Acid Production)

  • 조규홍;조윤경;홍승서;이현수
    • 한국미생물·생명공학회지
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    • 제23권1호
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    • pp.6-11
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    • 1995
  • In order to screen microorganism producing lactic acid with high productivity from nature, we used a medium containing 100 g/l glucose and selected several microorganisms producing more than 80 g/l L-lactic acid. We investigated their physiological characteristics and compared them. The best microorganism was identified as Lactobacillus casei subsp. rhamnosus. The optimum pH for growth and production of lactic acid was 6.0 and this strain showed the highest growth rate at around 30$\circ$C , but the optimum temperature for lactic acid production was 45$\circ$C . The growth was inhibited proportionally from 50 g/l to 300 g/l of glucose and the maximal cell mass increased according to increasing the concentration of corn steep liquor (CSL) protein up to 30 g/l. In batch fermentation for lactic acid production, we produced 128 g/l L-lactic acid with 20 g/l CSL protein and 150 g/l glucose in 35 hours. In pH-stat fed-batch fermentation, we were able to produce 183 g/l L-lactic acid.

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유기질비료와 토양 미생물제제의 시용이 배추(Brassiea campestris L.)와 상추(Lactuca sativa L.)의 수량에 미치는 영향 (Effects of Brassiea campestris L. and Lactuca sativa L. Yield by Application of Organic Fertilizers and Microorganisms)

  • 조성현;박태헌;장기운
    • 유기물자원화
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    • 제9권3호
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    • pp.88-92
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    • 2001
  • 돈분발효퇴비(P)와 혼합유박비료(B, F), 부산물퇴비(D) 4종의 유기질비료와 토양 미생물제제(M)의 시용 효과를 규명하기 위하여 배추와 상치를 공시작물로 시설하우스에서 재배하면서 생육 및 수량을 조사하였다. 유기질비료의 추천 시비량 처리구에서 수량이 가장 좋았으며, 과량 처리구에서는 비해가 발생하였으나, 미생물제제의 혼용에 의해 현저한 비해 감소 현상을 볼 수 있었다. 또한 같은 비종 및 동일한 시용량 처리구에서도 미생물제제를 혼용하였을 때 생육이 안정되었으며, 수량도 높은 경향을 보였다. 결론적으로 유기질비료의 과량처리는 비해가 발생할 수 있으며, 미생물제제의 시용으로 생육 및 수량이 향상되었다.

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화산력-시멘트 혼합 바이오 블록의 미생물 생장 특성 (Growth Characteristics of Microorganism on Lapilli-Cement mixed Bioblocks)

  • 박성용;박덕환;김현선;김정면;임현택;배수빈;김용성
    • 한국농공학회논문집
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    • 제57권1호
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    • pp.111-118
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    • 2015
  • This research aims to utilize lapilli from the Mt. Baekdusan as environmently-friendly construction material. First of all, the neutralizing method for fabricating lapilli-cement-mixed bioblock was examined. And then, by use of the neuralized bioblock with microorganism for water purification, the growth inhibition effect against the pathogenic coliform bacillus was evaluated. The result regarding growth inhibition effect on pathogenic coliform bacillus indicates that the pretreatment condition, which is a concurrent procession with aqueous solution of 10% di-ammonium Phosphate after water curing, led to pH degraded below 10 which was the target value. Therefore it was concluded that the method was effective on bio-block neutralization. The microorganisms purifying water and di-ammonium phosphate were detected through the examination for microorganism existence on the bioblock, therefore it was concluded that the bioblock composed of lapilli and cement is able to be utilized in various structures as an environment friendly construction material.

미생물의 비성장속도와 지연기의 측정을 통한 유해오염물질의 독성검사 (Toxicity Evaluation of Hazardous Contaminants by Measuring Lag Periods and Specific Growth Rates of a Test Microorganism)

  • 양진우;장덕진
    • KSBB Journal
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    • 제13권5호
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    • pp.469-476
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    • 1998
  • Among 31 water-born microbial strains isolated from various sites in Korea, strain DJ-4 was selected as a test organism for toxicity measurements in that its growth was completely inhibited by the presence of 668.4 mg/L of chloroform and 297.5 mg/L of toluene in the liquid LB medium whereas others did not. It was observed that lag periods and specific growth rates of DJ-4 batch vial cultures were prolonged and decreased, respectively, by phenol, benzene, toluene, ethylbenzene, p-xylene, perchloroethylene, trichloroethylene, and chloroform at the concentrations between 3.6 and 417.8 mg/L. There changes were found to be linear with respect to the concentrations of the toxic compounds. From the first-order regression equations, 50% effective concentrations (EC50${\mu}$ for concentrations of toxic compounds causing 50% decrease of specific growth rates and EC50lag for 50% increase of length of lag periods) were calculated for each compounds. By comparing DJ-4 EC50${\mu}$ values with Daphnia LC50's from a literature for benzene, ethylbenzene, toluene, and trichloroethlyene, it was concluded that microbial specific growth could be a new, fast, and reliable parameter for toxicity tests.

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친환경 유기질 비료 시용이 참당귀의 생육과 수량에 미치는 영향 (Effects of Eco-Friendly Organic Fertilizer on Growth and Yield of Angelica gigas Nakai)

  • 김영국;안태진;여준환;허목;박영심;차선우;송범헌;이경아
    • 한국약용작물학회지
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    • 제22권2호
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    • pp.127-133
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    • 2014
  • This experiment was carried out to investigate the effect of several organic compost on the growth and root yield of Angelica gigas Nakai with organic cultivation. After fertilizing the soil with organic fertilizer, the dry weight of liming fertilizer showed a slow change, while microorganism fertilizer decreased about 29% until 20 day after fertilizing. At 110 days after fertilizing, microorganism fertilizer decreased more than liming fertilizer. Liming fertilizer decomposed slowly, while microorganism fertilizer decomposed early on rapidly but gently after 20 days in decomposed rate of organic fertilizer. Dried root yields per 10a of A. gigas were not significance between 277.6kg in conventional fertilizer and 277.7kg, 280.5kg in N 1.5, N 2.0 times of microorganism fertilizer. Decursin and decursinol angelate contents in A. gigas were 9.08 ~ 9.07% from N 1.0 and N 1.5 times in liming fertilizer, and 7.94 ~ 8.12% from N 1.5 times and N 2.0 times in microorganism fertilizer, compared to 7.31% of conventional treatment.