• Journal of Environmental Science International
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• v.26 no.3
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• pp.393-399
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• 2017

In order to determine the optimal water intake point, the distribution of blue-Green algae and water quality factors in relation to the depth of the Mulgum and Maeri stations located downstream of the Nakdong River were investigated from Jun. 2015 to Sep. 2016. When the current surface water intake system was converted to the deep water intake system, Chl-a concentration and blue-Green algae were reduced by 64.1% and 80.5%, respectively. Microcystin-LR was reduced by 50% to 100%, while geosmin and 2-MIB of the odorant substances were reduced by 42.9% and 11.8%, respectively. The water quality factors such as pH, water temperature, TOC and COD were gradually decreased by 30% in deep water. Therefore, if we used the deep water intake system selectively in the summer season when blue-Green algae masses occur, the concentration of the influx of blue-green algae and its by-products can be expected to decrease, leading to reduced operation costs in tap water production and improved of raw water quality.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.1-1
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• 2017

녹조현상은 부영양화된 호수나 유속이 느린 하천에서 부유성의 조류(식물 플랑크톤)가 대량 증식하여 수면에 집적하게 되고 물의 색을 현저하게 녹색으로 변화시킴으로써 발생된다. 최근에는 이러한 녹조 현상이 광역화, 독성화, 장기화의 특성을 띠며 빈번히 발생되고 있다. 녹조현상은 독소를 발생시키는 남조류에 의해 수생식물에 악영향을 주는 것으로 알려져 있다. 예를 들면 독소에 의한 가축에의 영향, 생태계 파괴로 인한 생태학적인 문제, 산소결핍으로 인한 물고기 및 각종 수중생물 폐사 등의 심각한 문제를 야기한다. 또한 조류는 식수에서 맛과 냄새를 유발할 뿐 아니라 Microcystin-LR과 같은 유해한 독소를 배출하여 공중 보건을 위협한다. 이에 식수원으로 사용되는 하천의 조류 번식에 따른 대응방안 마련이 절실히 요구된다. 유입되는 조류로 부터의 정수처리 설비의 처리 부하를 줄이기 위해서는 취수시스템과 연계한 고속 전처리 조류 제거 시스템을 개발이 필요하다. 기존의 전기응집부상공정(Electro-Coagulation and Flotation, ECF)은 화학 약품(응집제) 투여량이 적은 이점이 있지만 비교적 긴 전기 분해 시간이 필요하여 기존 정수처리 시스템과 연계성에 있어 한계가 있다. 이에 본 연구는 전기 분해 시간을 줄여 유입된 조류를 수 초 내에 응집하여 1분 이내에 조류를 분리하는 초고속 조류 전처리 기술을 개발하였다. 개발된 기술의 현장적용 및 실험 결과, 응집과정이 없이도 Chlo-a는 약 45 %의 제거 효율을 나타났다. 또한 응집제의 투입 및 전극에 의한 부상시스템에 의해 Chlo-a가 약 80 %로 제거되는 것으로 나타나 빈번하게 발생되는 조류로부터 안정적인 물 공급을 위한 전처리 공정으로 활용이 가능할 것으로 판단된다.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.7-13
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• 2015

Hydrotrope-combined copper (HCC) is a copper ($Cu^{2+}$)-based algicide, which is combined with a hydrotrope that keeps copper ion in solution to improve performance. This study assessed the growth inhibition effect of HCC against Microcystis aeruginosa which is one of the most common toxic cyanobacterium in eutrophic freshwater environment. Various HCC doses, ranging from 5.5 to $550{\mu}g/L$ as $Cu^{2+}$, were applied to either BG-11 or 1/4 diluted medium with low- or high-inoculum density of M. aeruginosa. Growth inhibition was monitored based on a decrease in chlorophyll-a content in culture medium during the incubation. Results showed that HCC significantly inhibited the growth of M. aeruginosa in a dose-dependent manner. In case of 1/4 diluted BG-11 medium, HCC dose as low as $5.5{\mu}g$ $Cu^{2+}/L$ completely inhibited the production of chlorophyll-a by M. aeruginosa. It was found that HCC did not induce any significant release of microcystin-LR from M. aeruginosa. Acute toxicity of HCC was tested using Daphnia magna, and the 24-h $EC_{50}$ value was 0.30 mg/L as $Cu^{2+}$ which was much higher than the actual inhibition dose. Ames test was performed using Salmonella enterica serovar Typhimurium TA100, and HCC showed no increase in the number of revertant colonies. The result suggested that HCC does not have any mutagenic potential in the aquatic environment. In addition, no genotoxic effect of HCC was also confirmed based on the SOS ChromoTest using Escherichia coli PQ37. Therefore, HCC could be used as a relatively safe and effective pre- and post-treatment agent to control hazardous algal blooming in aquatic environments.

• KSBB Journal
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• v.22 no.5
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• pp.356-361
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• 2007

Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Although some cyanobacteria produce lethal toxins such as microcystins and anatoxins, some may be useful either for development into commercial drugs or as biochemical tools. Detection of unknown secondary metabolites was carried in the present study by a screening of 98 cyanobacterial strains from Cyanobiotech GmbH in order to establish a screening process, isolate pure substances and determine their bioactivities. A degenerated polymerase chain reaction technique as molecular approaches has been used for general screening of NRPS gene and PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%), respectively. A screening of interesting strains was performed by comparing PCR screening results with HPLC analyses of extracts. HPLC analysis for a detection of natural products was performed in extracts from biomass. 5 strains were screened for further scale-up processing. 7 pure substances were isolated from the scale-up cultures and tested for bioactivities under consideration to purity, amount and molecular weight of substances. One substance isolated from CBT 635 showed cytotoxic activity. This substance may be regarded as Microcystin LR.