• Title/Summary/Keyword: microbiological safety and quality

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Investigation of Microbiological and Physiochemical Quality for Irrigation Water used in Napa Cabbage Cultivation (배추 재배에 이용되는 농업용수의 미생물 오염도 조사 및 이화학성분 분석)

  • Yun, Bohyun;Kim, Min Kyung;Ryu, Jin Hee;Kim, Won-Il;Park, Byeong-Yong;Kim, Hyun-Ju;Lee, Seung-Don;Kim, Se-Ri
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.396-403
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    • 2017
  • The purpose of this study was to investigate water quality for irrigation water used in Napa cabbage cultivation. The water samples were analyzed for physiochemical and microbiological quality for a total of 111 samples including surface water (n = 75) and groundwater (n = 36) collected from five different regions where Napa cabbage is massively grown. As a conclusion, the levels of fecal indicators for surface water were higher than those for groundwater. The numbers of coliform from surface water and groundwater were 1.96-4.96 and 0-3.98 log MPN/100 mL, respectively. Enterococci were detected in 95% (72/75) of surface water samples and 22% (8/36) of groundwater samples. Besides, 97% (73/75) of surface water samples were observed being contaminated with Escherichia coli, and 22% (8/36) of groundwater sample was positive for E. coli. In the case of surface water, E. coli and coliform correlate to T-P, and enterococci showed relevance to the suspended solid (SS) and biochemical oxygen demand (BOD). In groundwater, fecal indicator bacteria showed relevance to the SS and chemical oxygen demand (COD). These results could be provided as fundamental date for establishing microbial standard of water used in leafy vegetables cultivation.

Quality Characteristics of Beverage Adding Onion Peel Extract (양파껍질 추출물 함유 음료의 품질특성)

  • Jeong, Eun-Jeong;Cha, Yong-Jun
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.1
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    • pp.11-19
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    • 2022
  • This study provided basic data for the commercialization of healthy functional beverages by examining the physicochemical characteristics, nutrient content, and microbiological safety of onion peel beverage. The total acid of onion peel beverage was 0.12 mg/g and the pH was 6.07. According to the storage period, the total acid decreased and the pH increased in all temperature ranges(25℃, 35℃, and 45℃). After 30 days of storage, it showed 0.06 mg/g in all temperature sections and maintained the pH 7 range. The total phenol content, which is a nutritional component of onion peel beverage, was 0.93 mg/g, flavonoid content was 0.25 mg/g, and quercetin content was 0.17 mg/g. The flavonoid content decreased according to the storage period, and in the case of storage temperature of 25℃, it contained 50% content up to 120 days of storage, but in the case of 35℃ and 45℃, it had a flavonoid content up to 90 days and 30 days, respectively. In the case of quercetin, a residual rate of about 50% was shown for 150 days of storage at 25℃ and 35℃. In the case of 45℃, it decreased to 35% at 30 days of storage. On the other hand, onion peel beverage maintained a viable cell count of less than 5 CFU/mL for 150 days of storage, and no coliform group was detected. As a result of analyzing the quality characteristics of onion peel beverage according to storage period, quality stability was confirmed in physicochemical characteristics and microbiological safety. Research on changes in biological activity according to low-temperature distribution or storage period is necessary.

Effects of Various Temperatures and Packaging Methods on the Storage Properties of Hanwoo Tenderloin (다양한 온도와 포장방법이 한우 안심의 저장성에 미치는 영향)

  • Jong-Hui Kim;Eun-Seon Lee;Mi-Hwa Oh
    • Journal of the Korean Society of Food Culture
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    • v.39 no.2
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    • pp.119-126
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    • 2024
  • This study was conducted to examine the microbiological quality indicators (total bacterial count and coliform count) and physicochemical quality indicators (pH, redness, volatile basic nitrogen [VBN] content) of meat according to various storage temperatures (-20~15℃) and packaging methods (wrap, vacuum). Based on these results, we proposed a safe consumption period. Redness, pH, and VBN content were not considered appropriate for setting the expiration date, as the redness and pH of the meat after spoilage were better than the standard values for both vacuum and wrap packaging (p<0.05). Additionally, the VBN content at 2 and 4℃ increased slightly (fresh level) until the initial time of spoilage (1.0×106 colony-forming unit [CFU]/cm2) and then increased rapidly thereafter. Therefore, the results were not consistent with microbial spoilage. When the decay point was evaluated based on the presence of microorganisms, vacuum packaging extended the storage period approximately 2.5-fold when compared with wrap packaging, and the meat could be stored at 2 or 4℃ for 40 or 23 days, respectively. Therefore, to evaluate meat quality, microbial indicators should be considered first. The microbiological standards proposed in this study can be used for safety management during the distribution of meat. However, to ensure meat safety, additional investigations of appropriate indicators of freshness must be conducted.

Microbiological quality of fresh cut fruits in Korea (국내 신선편이 과일의 미생물 품질 평가)

  • Kim, Myung-Ji;Cheigh, Chan-Ick
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.809-814
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    • 2021
  • This study was performed to evaluate the microbiological quality of fresh cut fruits in Korea. Forty freshly cut fruit samples were assessed for aerobic mesophilic count (AM), aerobic psychrophilic count (AP), total coliform, generic Escherichia coli, yeast and mold (YM), Bacillus cereus, Staphylococcus aureus, Salmonella spp., and E. coli O157:H7. The average value for AM, AP, and YM was 4.51, 5.35, and 4.31 log CFU/g, respectively. The average of the total coliform was 2.42 log CFU/g, and E. coli was not detected in all samples. For foodborne pathogen bacteria, B. cereus and S. aureus were detected in 2.5 and 7.5% samples, respectively, and Salmonella spp. and E. coli O157:H7 were not detected in all samples. Among the samples, pear generally had the highest contamination level. Therefore, to assure the safety of fresh cut fruits, low temperature and thorough hygiene management should be implemented.

Analysis of Microbiological Contamination in Kimchi and Its Ingredients

  • Lee, Ji-Hyun;Ha, Ji-Hyoung;Lee, Hae-Won;Lee, Jae Yong;Hwang, Ye-Seul;Lee, Hee Min;Kim, Sung Hyun;Kim, Su-Ji
    • Journal of Food Hygiene and Safety
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    • v.33 no.2
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    • pp.94-101
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    • 2018
  • Although Kimchi has health benefits, food poisoning associated with consumption of Kimchi has been frequently reported. Accordingly, microbiological properties of Kimchi (100 samples) and washing effects on microbial reduction against its ingredients (200 samples) were examined. Total aerobic bacteria, coliforms, Escherichia coli, Bacillus cereus, and Clostridium perfringens were quantified. In addition, B. cereus, Salmonella spp., Enterohemorrhagic E. coli, C. perfringens, Campylobacter jejuni/coli, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, and Yersinia enterocolitica were analysed qualitatively. Total aerobic bacteria count was approximately 1.4-9.0 log CFU/g, which were highest in ginger (8.8 log CFU/g), and Chonggak Kimchi (9.0 log CFU/g). The range of coliform counts detected in all raw materials was 0.5-7.3 log CFU/g and ginger showed the greatest number 7.3 log CFU/g among others. Contamination was decreased to 0.2-3.2, 0.3-2.7, and 1.0-3.9 log CFU/g for total aerobic bacteria, coliforms, and B. cereus, respectively, after washing. Minimising microbial contamination in Kimchi ingredients is necessary to ensure the safety of Kimchi. These results indicate that washing is a useful method to reduce bacterial contamination in Kimchi.

Gaseous Chlorine Dioxide Treatment to Produce High Quality Paprika for Export (고품질 수출용 파프리카 생산을 위한 이산화염소 가스 훈증 처리)

  • Kang, Ji Hoon;Park, Shin Min;Kim, Hyun Gyu;Son, Hyun Jung;Song, Kyoung Ju;Cho, Miae;Kim, Jong Rak;Lee, Jeong Yong;Song, Kyung Bin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.1072-1078
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    • 2015
  • To maintain high quality and microbiological safety of paprika during storage for export, paprika samples after harvest were treated with 75 ppmv chlorine dioxide ($ClO_2$) gas and stored at $8^{\circ}C$, which is the optimal storage temperature, for 30 days. $ClO_2$ gas treatment reduced initial populations of total aerobic bacteria in samples by 1.62 log CFU/g as well as yeast and molds by 1.45 log CFU/g compared to those of the control. During storage, weight loss of all samples increased, and samples treated with $ClO_2$ gas showed lower weight loss than the control. In addition, total soluble solid and total phenolic contents were not significantly different between the samples during storage, whereas vitamin C content and hardness of all samples decreased. Hunter L, a, and b values of paprika samples were not significantly different between the treatments. These results suggest that $ClO_2$ gas treatment can be effective for improving microbiological safety and maintaining high quality of paprika during storage.

Changes in Microbiological and Chemical Properties of Natural Water with the Storage Time and Temperature (시판 먹는 샘물의 저장 기간 및 온도에 따른 세균학적 및 화학적 품질 변화)

  • 박신인;이왕규;조윤정
    • Journal of Food Hygiene and Safety
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    • v.12 no.1
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    • pp.55-62
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    • 1997
  • This study was carried out to investigate the microbiological and chemical properties of natural water during storage. The water samples were taken at the time of purchase and the opened bottles and unopened bottles stored at the temperature of 4$^{\circ}C$, 18$^{\circ}C$, and 3$0^{\circ}C$. The bacterial content normally rose to 2.06$\times$102 CFU/$m\ell$ for the unopened bottles and 2.91$\times$102 CFU/$m\ell$ for the opened bottles after 2 weeks of storage, and 1.21$\times$102 CFU/$m\ell$ and 2.64$\times$102, respectively, after 24 weeks of storage. The number of viable cells of bacteria peaked more rapidly at the storage temperature of 3$0^{\circ}C$ than 18$^{\circ}C$. But the total samples were found to be negative for coliforms test during the study period. The average range of pH value was from 7.39 to 7.76. The results showed that the nitrates and chlorides satisfied the Korea Drinking Water Quality Standards during the storage period of 24 weeks. However, the undesirable changes of the taste and odor were detected within 2 weeks and 3 weeks, respectively.

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A Study on Microbiological Quality & Safety Control of Dongtae-Jeon(Pan-fried dish) and Rolled Egg in Packaged Meals(Dosirak) with Various Cooking Processes (시판 도시락 중 동태전과 달걀말이의 생산단계에 따른 품질관리에 관한 연구)

  • 김혜영;고성희
    • Korean journal of food and cookery science
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    • v.20 no.3
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    • pp.292-298
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    • 2004
  • This study researched the microbial change of quality according to various phases of product flow of Dongtae-Jeon (a pan-fried dish) and rolled egg in packaged meals. In order to carry out the study, the time required, temperature, water activity and microbial quality were measured at various phases of production flow of Dongtae-Jeon and rolled egg in packaged meals, and the effects of these factors on microbial multiplication was analyzed. According to the phases in product flow of Dongtae-Jeon, it was shown that the time required is 12.5hrs and water activity is distributed 0.932-0.980. These conditions were suitable for microbial multiplication. According to the phases in product flow of rolled egg, it was shown that the time required is 3.3hrs. In addition, qualitative analysis of pathogenic microorganisms (Salmonella spp., Vibrio parahaemolyticus, Staphylococcus aureus) detected no such microorganisms in any of the samples.

Microbiological Quality of Fresh-Cut Produce and Organic Vegetables (신선편의 샐러드와 유기농 채소류의 미생물학적 품질 및 식중독 미생물 오염도)

  • Jo, Mi-Jin;Jeong, A-Ram;Kim, Hyun-Jung;Lee, Na-Ri;Oh, Se-Wook;Kim, Yun-Ji;Chun, Hyang-Sook;Koo, Min-Seon
    • Korean Journal of Food Science and Technology
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    • v.43 no.1
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    • pp.91-97
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    • 2011
  • This study was performed to assess the microbiological quality and potential health risk of fresh-cut produce and organic vegetables sampled from supermarkets and department stores in Korea. A total of 96 samples comprised three types of fresh-cut produce (sprouts, mixed-vegetables, fruit) and three types of organic vegetables (lettuce, perilla leaf, green pepper). The samples were analyzed for total viable cell counts, coliforms, Enterobacteriaceae, Escherichia coli, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus, and Staphylococcus aureus. The microbiological counts of fruit were very low. Sprouts were highly contaminated by total viable cell counts ($8.3{\pm}0.57$ log CFU/g), Enterobacteriaceae ($7.1{\pm}0.76$ log CFU/g), and coliforms ($4.9{\pm}0.40$ log MPN/g), and showed a high incidence level of B. cereus ($2.9{\pm}0.48$ log CFU/g). Of the fresh-cut produce analyzed, six (13.6%) mixed-vegetable salads were E. coli positive. S. aureus was detected in only one sprout sample and one mixed-vegetable salad, and its contamination levels were under 2 log CFU/g, which is appropriate for Korean standards (<3 log CFU/g) of fresh-cut produce. Of the organic vegetables, lettuces were highly contaminated by total viable cell counts ($6.4{\pm}0.74$ log CFU/g), Enterobacteriaceae ($5.7{\pm}0.98$ log CFU/g), and coliforms ($3.7{\pm}1.72$ log MPN/g). Two (13.6%) organic lettuce and one (7.1%) perillar leaf sample were E. coli positive, and S. aureus was detected in one lettuce and two perilla leaf samples. Salmonella spp., Vibrio parahaemolyticus, and Listeria monocytogenes were not detected in any of the fresh-cut produce or organic vegetables analyzed.

The Microbiological Assessment of a University Foodservice Establishment, and Hazard Analysis for Quality Control of Fried Fish Cake Soup Preparation (대학 급식시설의 위생 실태조사 및 품질관리를 위한 연구 - 제 1 보 : 오뎅국을 중심으로 -)

  • Rew, Kyung;Kim, Jeong-Mi;Kwak, Tong-Kyung
    • Journal of Nutrition and Health
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    • v.18 no.4
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    • pp.283-292
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    • 1985
  • A sanitary quality of a university foodservice establishment was assessed in terms of time and temperature, pH and Aw, and microbiological evaluation. Critical control points during various phases in product flow of fried fish cake soup were identified using hazard analysis concept. The results are summarized as follows : 1) Time and temperature data indicated that phases of holding ingredients at room temperature after cooking, and hot - holding of soup before assembly were critical. 2) pH and Aw values were in favorable for microbial growth. 3) Microbiological data indicated that microbial quality of ingredients was in poor condition. A holding practice of cooked ingredients at room temperature might provide the chance for microbial contamination and multiplication. Hot - holding time and temperature of soup should be strictly controlled to assure the food safety, since the microbial quality of serving food can be directly influenced by the hot - holding temperature of soup. 4) Microbiological test results for food containers, equipments, working surfaces, and supplies indicated that sanitary conditions of earthenware, gloves, wiping cloths and cutting board should be improved promptly. 5) Critical control points identified were : ingredients, pre - preparation, post - preparation, and holding before assembly and service. 6) Guidelines for the effective quality control program for this operation were suggested.

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