• 한국수산과학회지
• /
• 제33권1호
• /
• pp.70-74
• /
• 2000

우뭇가사리로부터 조한천을 조제하고, 이를 실제 산업에 적용할 목적으로 $0.4\;{\mu}m$ 기공을 가지는 여과 막을 microfiltration한 후, $50{\circ}C$의 온수로써 가용성 물질을 제거하고, 0.25 N NaOH로 $70{\circ}C$에서 2시간 처리하는 알칼리 처리를 거쳐 미생물 배지용 한천을 조제하였다. 이화학적 특성을 측정한 결과에 의하면, 겔강도는 $460g/cm^2$에서$738g/cm^2$으로 상승하였고, 점도는 12 cp에서 5.2 cp로, 황산기 함량은 $1.8{\%}$에서 $0.4{\%}$로, 그리고 이수율은 $10.3{\%}$에서 $5.4{\%}$로 저하하여 제품의 품질이 월등히 향상되었다. 상용되는 미생물 배지용 한천은 겔강도가 $358{\~}368g/cm^2$, 점도는 8 cp, 황산기 함량은 $2.18{\~}2.39{\%}$ 및 시너레시스율은 $11.3{\~}12.4{\%}$로 분석되어 본 실험법으로 정제한 한천이 물성 및 성분에 있어서 휠씬 우수 하였다.

• 미생물과산업
• /
• 제26권2호
• /
• pp.2-12
• /
• 2000

• 미생물학회지
• /
• 제27권3호
• /
• pp.310-315
• /
• 1989

식물의 잎에 세균성 정무늬병을 야기시키는 식울 병원성균인 Pseudomonas syringae pv. tuhaci는 tabtoxin 이라는 phytotoxin 을 생성하는데 이 toxin을 미생물학적으로 간편하게 검색하는 방법플 여러가지 지시균주를 사용하여 각족 배지에서 검토하였다. Minimal A agar medium에서는 tabtoxin 검색에 Agrobactcrium tumefaces가 가장 유용한 균주였으며 minimal glucose agar m$\varepsilon$dium 에서도 역시 마찬가시의 견과를 얻였다 Complex agar medium 에서는 사용된 모든 지시균주에 대해 증식저지환이 형성되지 않아, tabtoxin이 생성되지 않음알수 있었다. 배양온도에 따른 tabtox의 생성능은 $20^{\circ}C$ 빛 $30^{\circ}C$ 에서 최적이였으며 배양시간이 경과함에 따라 tabtoxin 생성량이 증가 하였다. Glutamine을 minimal glucose agar medium에 첨가하여 tabtoxin 에 대한 지시 균주의 반응은 첨 가한 glutamine 의 양이 증가할수록 tabtoxin에 의한 생육억제가 감소함을 알 수 있었다.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2002년도 추계학술대회
• /
• pp.222.2-222.2
• /
• 2002

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 1998년도 제38회 춘계학술발표대회
• /
• pp.45.2-45.2
• /
• 1998

• 대한의생명과학회지
• /
• 제29권4호
• /
• pp.249-255
• /
• 2023

Transporting clinical samples for microbiological testing requires a proper transport medium that guarantees the survival of microorganisms. Therefore, the aim of the study was to determine the ability of Amies Transport Medium (ATM) to maintain the viability of microorganisms in clinical specimens and its suitability as a transport medium for microbiological testing. This study evaluated the performance of swab provided by KS Co., Ltd. for three groups of bacteria comprising aerobic and facultative anaerobic bacteria, anaerobic bacteria, and fastidious bacteria, according to the Clinical and Laboratory Standard Institute (CLSI) 8.11.2. The ATM stability test was conducted by dividing the medium into two groups based on the product expiration date of use. All tested media, A and B (the date of manufacture and expiration date are different) showed ≥5 CFU, and there was no significant difference in the result values of Category A and Category B with different serial numbers for each test. The results of this experiment when cross-checked with the guidelines suggest that ATM is a suitable transport medium for microbiological testing, as it maintains the viability of microorganisms and is suitable for overgrowth trials. In addition, compared to the number of CFUs at the origin, the number of CFUs did not increase by more than 1 log after storage. These results have important implications for the development of transport media that can guarantee the survival of microorganisms in clinical specimens.

• 한국균학회지
• /
• 제44권2호
• /
• pp.87-93
• /
• 2016

From 182 non-pathogenic wild yeast isolates from flowers, Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1 were selected for potent ${\gamma}$-aminobutyric acid production and microbiological characteristics were investigated. Pichia silvicola UL6-1 formed ascospores and pseudomycelia. The strain was also halotolerant, growing well in 5% NaCl-containing yeast extract-peptone-dextrose (YPD) medium. Sporobolomyces carnicolor 402-JB-1 did not form ascospores or pseudomycelia and grew well on 10% glucose-yeast extract-peptone medium.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
• /
• pp.196.1-196
• /
• 2005

• 미생물과산업
• /
• 제19권3호
• /
• pp.41-45
• /
• 1993

The cultural conditions of the osmophilic red color yeasts (Strain L$_{1}$, L$_{2}$, L$_{3}$ and L$_{4}$) isolated and identified in the previous report were examined and the results obtained were as follows ; 1. The optimum medium for growth of these osmophilic red color yeasts was soy sauce medium. 2. These strains were grown exceedingly well on the medium containing 3 percent of NaCl but somewhat restrained on the medium containing 6% or more. 3. The optimum temperautre for growth of these strains was 25.deg.C and their lethal temperature was 68.deg.C(treatment for 5 minutes). 4. The optimum pH for growth of these strains was 6.0.

• 미생물학회지
• /
• 제29권5호
• /
• pp.267-269
• /
• 1991

The hyphal tip laccase of Coprinus congregatus which is a membrane-associated enzyme and shows diffdrdnt banding patterns of PAGE analysis when compared with the enzyme of liquid culture (Choi et al. 1987) has been successfully secreted to culture medium in liquid shake culture by lowering the pH of medium to 4.0. When the fungus is cultivated in YpSs(pH 4.0) liquid, only the hyphal tip laccase is found in the medium after 6 hr incubation and there is no liquid-type enzyme when examined by PAGE analysis.