• 한국식품영양과학회지
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• 제36권7호
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• pp.938-943
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• 2007

향후 소비가 더욱 증가할 것으로 예상되는 즉석섭취식품 중 샌드위치를 대상으로 제조공정의 특성상 생물학적 위해 중 높은 빈도로 검출될 것으로 예상되는 황색포도상구균에 대해서 정성적${\cdot}$정량적 검출실험을 실시하였다. 분석시료는 편의점, 대형할인마트, 샌드위치 전문점, 개인운영제과점, 패스트푸드점, 급식소에서 판매되는 샌드위치를 대상으로 하였다. 분석결과 전체 174개의 시료 중 18개, 전체 10.3%에서 황색포도상구균이 검출되었으며 검출량은 평균 1.74 log CFU/g으로 0.30 log CFU/g에서 최대 4.08 log CFU/g까지 검출되었다. 황색포도상구균의 계절에 따른 검출량은 여름철(3.24 log CFU/g)이 겨울철(1.10 log CFU/g)에 비해서 3배 정도 많아 통계적으로 유의적인 차이가 있었다(p<0.001). 영국 PHLS의 즉석섭취식품의 황색포도상구균에 대한 미생물 가이드라인을 기준으로 평가했을 때 본 실험의 전체 분석 대상 시료 중 95.4%가 허용할 수 있는 수준이었다. 또한 햄 치즈샌드위치에 대한 독소형성 확인 실험 결과 4.95 log CFU/g 이상으로 황색포도상구균이 증식되지 않으면 독소가 생성되지 않는다는 것을 알 수 있었다. 샌드위치의 황색포도상구균에 대한 정량적 오염도 분석에 대한 본 실험 결과는 샌드위치를 포함한 즉석섭취식품의 정량적 위해평가를 위한 기초자료로써 활용될 수 있을 것으로 사료된다.

• 대한영양사협회학술지
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• 제13권4호
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• pp.389-406
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• 2007

This study was conducted to estimate the safety level of non-cooking and cooking processed foods to propose the sanitary management of foods donated to foodbanks. The time and temperature were measured and the microbial levels of aerobic plate counts (APC), coliforms, E. coli, Salmonella spp., S. aureus, B. cereus, and E. coli O157:H7 were analyzed on ten food items donated to seven foodbanks. The amount of cooked foods donated to each foodbank was about 10 to 40 servings. All foodbanks hired a supervisor and had at least one refrigerator/freezer and one temperature-controlled vehicle, but only four foodbanks had the separate offices to manage the foodbank operation. The flow of donated foods was gone through the steps; production, meal service and holding at donator, collection by foodbank, transport (or holding after transport) and distribution to recipients. After production, the levels of APC of both non-cooking and cooking processed foods were complied with the standards by Ministry of Education & Human Resources Development, and were not increased till distribution. Only the level of coliforms in dried squid & cucumber salad (1.5×$10^3$ CFU/g) was not met the standards. E. coli and other pathogens were not detected in all tested samples. The microbial levels of delivery vessels and work tables were satisfactory, but the APC levels of two of four tested serving tables (6.9×$10^3$ and 5.3×$10^3$ CFU/100$cm^2$) and the coliforms level of one (1.1×$10^3$ CFU/100$cm^2$) were over the standards. The air-borne microflora level in serving room was estimated as satisfactory. It took about 3.0 to 6.5 hours from after-production to distribution and the temperatures of donated foods were exposed mostly to temperature danger zone, which had a high potential of microbial growth. These results imply that a checklist to monitor time and temperature in each step should be provided and the employees involving foodbank operation should be properly educated to ensure the safety of donated foods.

• 한국수산과학회지
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• 제50권4호
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• pp.352-358
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• 2017

The object of this study is to assess physicochemical and microbiological hazards involved during the processing of shellfish products such as oysters, clam, and mussels. Samples including raw materials, intermediates, and final products in the processing process were collected from seven simple-processed shellfish facilities. In the samples obtained from the facilities, viable cell count and coliform group were detected less than that of the Korean Government guidelines. In addition, the high-risk bacterial pathogens such as Escherichia coli O157:H7, Salmonella sp., and Listeria monocytogenes were not identified in raw materials and final products. However, the low-risk pathogens, Staphylococcus aureus and Vibrio parahaemolyticus, were detected in some final products. The level of heavy metal content in the final products tested in this study meets the recommendations by the Korean Government standard guidelines. No foreign materials in the final products were also observed. Considering these results, it was concluded that no significant food hazards exists in the processing process of simple-processed shellfish products. However, it is essential to improve the food safety control in the shellfish processing facilities since S. aureus, a personal sanitary indicative bacterium, was continuously detected over seven simple-processed shellfish facilities.

• 한국식품위생안전성학회지
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• 제1권1호
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• pp.77-95
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• 1986

In 1962 the governing bodies of FAO and WHO approved the establishment of a joint FAO/WHO Food Standards Programme, the creation of a jointly sponsored body to be known as the Codex Alimentarius commission to implement the Programme. It can reasonably be claimed that the Commission has assumad the leading role in establishing internation food standards throughout the world. The Codex Committee of Food Hygiene has received much advice and assistance from other international organization which have been working in this field for a number of years. In particular, it has received valuable background documentation from the International Commission on Microbiological Specifications for Foods(ICMSF) which was set up by the International Association of Microbiological Societies(IAMS), and also from the International Organization for Standardization (ISO). Nevertheless, in spite of the information supplied by governments and research bodies in this field, microbiological standards have proved to be a highly controversial subject from the point of view of Codex standards. When it is decided to establish a microbiological standard for a food or class of foods, the following technical and administrative aspects must be considered: 1) The standard should be based on factual studies and serve one or more of the following objectives: (1) to determine the conditions of hygiene under which the food should be manufactured; (2) to minimize the hazards to public health; (3) to measure the keeping quality and storage potential of the food 2) The standard should be attainable under practicable operating and commercial conditions and should not entail the use of excessive heat treatment or the additions of extra preservatives. 3) The standard should be determined after investigation of the processing operation. 4) The standard should be as simple and inexpensive to administer as possible, the number of tests being kept to a minimum. 5) Details of methods to be used for sampling, examining and reporting should accompany all published microbiological standards. 6) In establishing tolerance levels for the permissible number of defective samples, allowance should be made for sampling and other variations due to differences in the laboratory methods. The following additional points should be kept in mind: 1) It is not satisfactory to establish one set of microbiological standards for a miscellaneous group of foods, such as“frozen foods”or“precooked foods”. 2) Microbiological standards should be applied first to the more hazardous types of food on the basis of experience of expected microbiological levels, taking into account variations in composition, processing procedures, and storage. 3) When a standard is established, there should be a definite relationship between the standard and the hazard against which it is meant to protect the public. 4) The sensitivity, reliability, and reproducibility of the sampling and analytical methods should be compared in different laboratories and the methods to be used should be specified in detail as part of the standard. 5) Tolerances should be included in the standard to account for inaccuracies of sampling and analysis. 6) Standards should be applied on a voluntary basis before compliance is made mandatory.

• 한국식품위생안전성학회지
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• 제23권4호
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• pp.285-290
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• 2008

본 연구에서는 대표적인 즉석섭취식품 5종(김밥, 초밥, 회, 샐러드, 샌드위치)에 대한 미생물 모니터링 검사를 실시하여, 이들 제품에 대한 위생 안전성 확보를 위한 기초자료를 제공하기 위해 진행되었다. 이를 위해 전국의 대형 백화점, 할인점, 슈퍼 등지에서 판매되는 즉석섭취식품 5종, 총 440품목을 수거하여 위생지표세균 검사 및 식중독균 검사를 실시하였다. 제품별로는 초밥이 20.4%로 가장 높은 미생물 검출율을 보였으며, 그 다음으로 13.3%의 김밥, 12.5%의 회, 6.9%의 샐러드, 2.8%의 샌드위치의 순서로 나타났다. 검출된 미생물을 살펴보면 황색포도상구균이 6.6%로 가장 많이 검출되었으며, 다음은 2.7%의 대장균군, 2.3%의 리스테리아균, 0.7%의 바실러스 세레우스균, 0.5%의 대장균 순서로 검출되었으며, 장염비브리오균과 살모넬라균은 검출되지 않았다. 검출된 황색포도상구균의 정량분석 결과는 전체적으로 $200{\sim}5,500\;cfu/g$의 분포를 보였으며, 평균적으로는 600 cfu/g을 나타내었다.

• 한국식품위생안전성학회지
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• 제27권4호
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• pp.420-426
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• 2012

본 연구는 당면의 원 부재료 및 제조공정별 위해요소분석을 실시하여 현장에 적용할 수 있는 HACCP 관리를 위한 과학적 근거자료를 마련하고자 하였다. 원 부재료, 공정별, 최종 제품, 기기 및 기구류, 환경으로부터 샘플을 수집하고, 미생물 분석은 일반세균, 대장균군, 대장균, 장내세균과 병원성 세균 5종(B. cereus, E. coli O157:H7, L. monocytogenes, Salmonella spp., S. aureus)에 대해 실시하였다. 원재료인 고구마 전분은 일반세균 3.02 log CFU/g로 나타났고, 대장균군, 장내세균은 불검출되었다. 키토산과 냉각수에서 각각 일반세균 3.47 log CFU/g, 2.72 log CFU/g로 검출되었다. 코팅수는 일반세균, 대장균군, 장내세균이 각각 6.45 log CFU/g, 4.13 log CFU/g, 4.73 log CFU/g로 검출되었다. 반죽은 일반세균, 대장균군, 장내세균 각각 4.31 log CFU/g, 2.71 log CFU/g, 2.88 log CFU/g으로 나타났으며, 호화 후 시료에서 일반세균 1.63 log CFU/g으로 감소하였다. 그러나 호화 이후의 공정부터 꾸준히 증가하여 최종 제품까지 일반세균 수가 약 5 log CFU/g로 일정한 수준을 유지하였다. 같은 업체에서 생산된 제품이라도 물리적인 조작이 추가된 제품에서 미생물 오염도가 높게 나타났다. 모든 시료에서 대장균과 병원성 세균은 검출되지 않았다. 기기 및 기구류 중 면대와 면을 걸어두는 기구에서 각각 일반세균 1.83-5.74 log CFU/$100cm^2$, 3.34-4.48 CFU/$100cm^2$로 검출되어 불만족 수준으로 확인되었다. 따라서 교차오염을 방지하기 위해 기기 및 기구류의 적절한 세척 소독 관리가 필요한 것으로 사료된다. 구역별로 공중 낙하균의 오염도를 분석한 결과, 반죽실, 절단구역, 숙성실, 포장실은 청정구역으로 만족범위, 타개실과 건조실은 허용범위로 나타났다. 외부에 노출되어 있는 해동실의 경우 검출균수가 높게 나타났으며 문이나 개폐장치로 추가적인 관리가 필요한 것으로 보인다. 앞선 실험결과를 바탕으로 호화 공정을 미생물학적 관리 지점으로 관리해야 하며 교차오염 방지와 선행요건 프로그램으로 관리되어야 할 것이다.

• 한국식품조리과학회지
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• 제27권2호
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• pp.61-71
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• 2011

This study was conducted to estimate the shelf-life of Kimbab manufactured using a Hazard Analysis and Critical Control Point (HACCP). We performed a microbiological verification after applying the HACCP plan to Kimbab. Additionally, the shelf-life of Kimbab at each holding temperature was calculated as a regression equation between the aerobic plate counts and holding time during the storage period. The critical control points of the HACCP plan, that were applied to Kimbab, included: cold-holding of refrigerated foods, checking the endpoint cooking temperature of heated food, and cold-holding of cooked foods. As a result of the microbiological verification of Kimbab, the aerobic plate counts averaged 3.46 log CFU/g. In contrast, the coliforms, E. coli, Staphylococcus aureus, and Salmonella spp. were not detected in any of the samples. The estimated shelf-life of Kimbab was calculated to be 45 hours at $10^{\circ}C$, 29 hours at $15^{\circ}C$, 6 hours at $25^{\circ}C$ and 3 hours at $35^{\circ}C$. In conclusion, manufacturers should apply a prerequisite program and a HACCP system for a safe consumption of ready-to-eat foods and label products with a proper shelf-life. Distributors should control the proper holding time-temperature until sale and consumers should eat immediately after purchasing ready-to-eat foods.

• Safety and Health at Work
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• 제10권4호
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• pp.428-436
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• 2019

Background: Metalworking fluids (MWFs) are mixtures with inhalation exposures as mists, dusts, and vapors, and dermal exposure in the dispersed and bulk liquid phase. A quantitative risk assessment was performed for exposure to MWF and respiratory disease. Methods: Risks associated with MWF were derived from published studies and NIOSH Health Hazard Evaluations, and lifetime risks were calculated. The outcomes analyzed included adult onset asthma, hypersensitivity pneumonitis, pulmonary function impairment, and reported symptoms. Incidence rates were compiled or estimated, and annual proportional loss of respiratory capacity was derived from cross-sectional assessments. Results: A strong healthy worker survivor effect was present. New-onset asthma and hypersensitivity pneumonitis, at 0.1 mg/㎥ MWF under continuous outbreak conditions, had a lifetime risk of 45%; if the associated microbiological conditions occur with only 5% prevalence, then the lifetime risk would be about 3%. At 0.1 mg/㎥, the estimate of excess lifetime risk of attributable pulmonary impairment was 0.25%, which may have been underestimated by a factor of 5 or more by a strong healthy worker survivor effect. The symptom prevalence associated with respiratory impairment at 0.1 mg/㎥ MWF was estimated to be 5% (published studies) and 21% (Health Hazard Evaluations). Conclusion: Significant risks of impairment and chronic disease occurred at 0.1 mg/㎥ for MWFs in use mostly before 2000. Evolving MWFs contain new ingredients with uncharacterized long-term hazards.

• 한국식품저장유통학회지
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• 제13권2호
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• pp.234-240
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• 2006

대구지역 대형식품접객업소 한식, 양식, 일식, 중식 각 3곳씩 12개 업소를 대상으로 조리기기 및 용기(칼, 도마, 행주, 집게, 바구니 등),조리종사자 손 및 조리 작업환경에 대한 미생물학적 위해를 분석하였다. 일부 한식당 조리수와 음용수의 일반세균 및 대장군균수가 $10^5\;CFU/mL,\;10^2{\sim}10^3\;CFU/mL$범위로 검출되어 기준치에 부적합하였다. 특히 끓여서 식힌 보리차를 제공할 경우 음용수에 대한 미생물적 수치가 높은 것으로 나타났으며, 일부 양식당과 일식당의 음용수도 기준치에 부적합하였다. 모든 유형의 업소에서 대부분의 조리기구의 일반세균 및 대장균군수가 기준치인 $2.5{\times}10^3\;CFU/100cm^2,\;1.0{\times}10^1\;CFU/100cm^2$를 초과하여 위생상태가 매우 불량한 것으로 나타났다. 특히 일부 중식당의 칼, 도마에서 E. coli가 $10^1{\sim}10^2\;CFU/100cm^2$ 검출되어 이에 대한 시정조치가 필요하였다. 대부분 업소에서 맨손으로 작업하였으며 조리종사자의 손의 일반세균 수 범위는 $10^2{\sim}10^7/hand$, 대장균군의 범위는 $0{\sim}10^6/hand$로 그 수치가 높아 손에 의한 음식물의 오염 위험도가 높은 것으로 나타났다. 또한 일부 한식당 조리종사자 손에서 황색포도상구균이 $1.3{\times}10^3/hand$로 검출되어 조리종사자의 손의 위생상태가 불량한 것으로 조사되었다. 작업대와 주방에서의 일반세균 및 진균의 공중낙하균이 최고 52 CFU/plate/10 min, 19 CFU/plate/10 min로 검출되어 작업환경 오염도가 높았으며 일부 업소에서는 냉장고 내의 공중부유물 오염도 또한 높은 것으로 나타나 식품접객업소의 작업환경에 대한 전반적인 개선이 요구되었다.

• 한국식품조리과학회지
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• 제13권5호
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• pp.592-601
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• 1997

The purposes of this study were to develop Hazard Analysis Critical Control Point-based standardized recipe applicable to cook/chilled soy sauceglazed mackerel and to evaluate the qualities related to the product flow of this item. After conducting experimental cooking, preliminary test, and analysis of recipes, critical control points were identified, control methods were determined, and HACCP-based recipe was standardized. At each critical control point, time-temperature profile was recorded and microbiological analysis (total aerobic plate counts, psychrotrophic plate count, coliform, and fecal coliform count), chemical analysis (pH, acid value and volatile based nitrogen (VBN)) and sensory evaluation of the item were done. Time-temperature data showed that the time the menu item had passed through temperature danger zone (5∼60$^{\circ}C$) during all phases was 60 min. At rapid cooling, but after cooling at room temperature, the temperature of this menu item did not drop below the ambient temperature. The results of microbiological test were negative throughout all phases following cooking and the results of chemical analysis did not change significantly in terms of storage periods except for VBN which increased on 7th day significantly(p<0.05). After steam/convection oven reheating and microwave oven reheating, the sensory score of the only appearance decreased significantly related to the storage time of overall quality profiles. But significant differences were not detected according to two reheating methods. In conclusion, this HACCP-based recipe was considered as an effective tool for assuring microbial as well as sensory quality of this cook/chilled item.