• Title/Summary/Keyword: microbial test

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Study on the Activation of Microbial Products by Using the Leachate (침출수를 이용한 미생물 제제의 활성에 관한 연구)

  • 이장훈;정준오;남명흔
    • Journal of Environmental Health Sciences
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    • v.24 no.3
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    • pp.70-76
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    • 1998
  • Activation bacteria, identified from commercial microbial products, were applied to leachate treatment. Total seven strains of bacteria Enterobacteriaceae spp. (5), Bacillus sp. (1), Aeromonas sp. (1) were seeded in the leachate and cultured in the shaking incubator at 25$^{\circ}$C and 250 rpm. While cultured, they were sampled in given time intervals and the removal rates of SS, COD, BOD, T-N.and T-P were measured an indicators of leachate treatment. Through the screening test, four of 7 strains of bacteria were considered to be effective and they were named as "effective group". The capability of leachate treatment was observed on three different groups of bacteria single, effctive, and total mixed. The result showed that the removal rates of COD and SS for the total mixed group were 64 and 71% respectively. BOD removal rate was reached nearly 99% by seeding of effective griup and removal rates of T-P and T-N were 83 and 82% respectively. However seeding of single strain was less effective than that of any mixed group in leachate treatment.

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A Study on the Diffusion and Removal of Airborne Microorganism Pollution in Multistoried Apartment by the Multi-Zone Simulation (멀티존 시뮬레이션에 의한 공동주택의 미생물 오염원 확산과 제거에 관한 연구)

  • Hong, Jin-Kwan;Choi, Sang-Gon
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.4
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    • pp.291-298
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    • 2007
  • The purpose of this study is to evaluate the efficacy of multizone simulation that enables to grasp of details about microbial contaminant problem in an multistoried apartment. We used actual indoor test data to figure up microbial contaminant level as initial value for the multizone simulation and estimated the various effects of indoor occupant infected with germs such as bacteria and fungus and the performance of air sterilization by using multizone simulation in substitute for infeasible experimental approach. The results show that natural ventilation make ourselves generally useful for removing indoor microbial contaminants. The results also show that the performance of air sterilization reach the maximum in the case of using mechanical ventilation and UVGI air sterilizer. The conclusion is that this multizone simulation is useful tool for actual design method for immune building systems.

Effects of High Relative Humidity on Weight Loss, Color Change, and Microbial Activity of Tomatoes during Refrigerated Storage

  • Pai, Tongkun
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.250-253
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    • 2000
  • The effects of high relative humidity (RH) on the physicochemical properties and microbial activity of mature green tomatoes ('Dombito') during refrigerated storage were determined at three temperatures (5, 10, and $15^{\circ}C$) and four different RH levels (91, 94, 97, and 99%). At each temperature, the weight loss rates of tomatoes at different levels of RH were significantly (p<0.05) different from each other. For the samples stored at $10^{\circ}C$, the weight losses were generally higher than those for the samples at $15^{\circ}C$ within the same RH level (i.e., greater vapor pressure deficit). The color change rates ('a' value) showed positive slopes, indicating that the tomato color was changing from green to red. Neither bacteria nor fungi caused visible damages to the samples, and the microbial counts were below 650 colony forming units/$cm^2$ during the test period.

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Effectiveness of external agents in polluted sedimentary area

  • Alam, Md. Mahabub;Haque, Md. Niamul;Cho, Daechul;Kwon, Sung-Hyun
    • Environmental Engineering Research
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    • v.21 no.1
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    • pp.52-57
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    • 2016
  • Sediment is a useful natural source but deteriorated continually by anthropogenic and industrial sources. Therefore, it is imperative to search a suitable method for improving or restoring sediment quality. Sediment has been tested to identify the effects of some external agents on a polluted area for 28 days. Chemical analysis and total viable counts (TVC) test have been conducted for 4 days interval to assess their performance. The analyses of chemical oxygen demand (COD), acid volatile sulfide (AVS), total phosphorous (T-P), total nitrogen (T-N) indicate that the chemical agents was more efficient to improve sediment quality whereas the microbial agent was more efficient for nutrient releasing from sediment. Oxygen releasing property of the chemical agent was thought to be providing with more congenial environment for the higher growth of the bacterial community than the direct application of microbial agents.

Genomic Fingerprinting of genera Bifidobacterium using Microbial Uniprimer Kit

  • Hwang, Young-Chol;Park, Jong-Sun;Kang, Byoung-Yong;Choi, Sung-Sook;Kim, Kyung-Jae;Ha, Nam-Joo
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.163.2-163.2
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    • 2003
  • The genera Bifidobacterium is a member of the normal intestinal flora in humans, and important in food industry. In order to test the genetic identity of this bacterial genera, four primers originated from rice genome (SRILS Microbial $UniPrimers^{TM}$ kit) were used in molecular typing of 7 Bifidobacterial species and 20 isolates from various source. SRILS Microbial $UniPrimers^{TM}$ kit were effectively applied to genomic fingerprinting of various organism such as plant, animal and microorganism. (omitted)

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The Examination of Mortar Durability by Microbial Biomineralization (미생물의 생체광물형성작용에 따른 모르타르 내구성 검토)

  • Kim, Sung-Tae;Chun, Woo-Young;Kim, Wha-Jung
    • Proceedings of the Korea Concrete Institute Conference
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    • 2009.05a
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    • pp.525-526
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    • 2009
  • On this paper we induce calcite($CaCO_3$) precipitation using microbial biomineralization of the Sporosarcina pasteurii and evaluate required performance evaluation by adjusting it to mortar. As a result carbonation normal mortar test piece(C3S-W) and mortar test piece(C3S-S.p) mixed with Sporosarcina pasteurii, reaction of C3S-S.p was late than C3S-W. Also, in the case of carbonation experiment of C3S-S.p curing in the Urea-CaCl2 aqueous solution(Medium) during 28days and durability of the C3S-W, durability of the mortar test piece(C3S-S.p) mixed with Sporosarcina pasteurii become higher than normal mortar test piece(C3S-W).

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Effect of quaternary ammonium compounds on microbial contamination levels in dental clinics (치과 진료실의 미생물 오염도에 대한 4차 암모늄 화합물의 효과)

  • Baik, Sae Yun;Park, YounJung;Choi, Seung Ho;Kim, Hee Jae;Kim, Seong Taek
    • Journal of Dental Rehabilitation and Applied Science
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    • v.36 no.1
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    • pp.55-60
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    • 2020
  • The aim of this study was to investigate the change of microbial contamination levels in the different areas and at the different time points after application of a quaternary ammonium compound (QAC) that has mechanical antimicrobial effect. The microbial contamination levels were measured in three different areas; unit chair handle, spit sink area and hand piece holder at different time points using adenosine triphosphate (ATP) monitoring system and ATP surface test kit. Hand piece holder showed the highest level of microbial contamination. In most of the clinics, QAC significantly reduced the levels of microbial contamination, and maintained antimicrobial activity for 4 to 6 months. QAC may be used effectively in dental clinics due to the duration of antimicrobial effect and the minimal exposure of chemicals and further studies are needed with large sample size.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

  • Lee, Jung-Sook;Jung, Min-Chul;Kim, Woo-Sik;Lee, Keun-Chul;Kim, Hong-Joong;Park, Chan-Sun;Lee, Hun-Joo;Joo, Yun-Jung;Lee, Kun-Jong;Ahn, Jong-Seog;Park, Wan;Park, Yong-Ha;Mheen, Tae-Ick
    • Microbiology and Biotechnology Letters
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    • v.24 no.2
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    • pp.234-241
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    • 1996
  • Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

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Solidification of Sandy Soils using Cementation Mechanism of Microbial Activity (미생물활성에 의한 시멘테이션 작용을 이용한 모래지반의 안정화)

  • Kim, Ki-Wook;Yun, Sung-Wook;Chung, Eu-Jin;Chung, Young-Ryun;Yu, Chan
    • Journal of The Korean Society of Agricultural Engineers
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    • v.56 no.6
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    • pp.169-176
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    • 2014
  • To evaluate bio-cementation of microbial on sands, laboratory test was conducted using acrylic cubic molding boxes ($5cm{\times}5cm{\times}5cm$). It was incubated the microbial, called Bacillus Pasteurii, according to Park et al (2011, 2012). and applied 50ml each specimen. Two type of sand samples used were Jumoonjin sand and common sand (well graded). These sands were molded in acrylic boxes with the relative density of 30 % and 60 % respectively. Microbial were poured onto the samples molded in acrylic boxes and cured at the room temperature and humidity. After 7, 14 and 21days, it was measured the compressive strength, pH, EC, and density and it were observed SEM and XRD to verify the effect of bio-cementation. It was found that bio-cementation was increased a strength of sands and it was appeared that strengths were related to the type of sand and relative density. Therefore it was confirmed the solidification of sands using the bio-cementation by microbial activation and the usefullness of acrylic molding boxes when tests were conducted on the soil of sands.

Symbiobacterium toebii Sp. nov., Commensal Thermophile Isolated from Korean Compost

  • Sung, Moon-Hee;Bae, Jin-Woo;Kim, Joong-Jae;Kim, Kwang;Song, Jae-Jun;Rhee, Sung-Keun;Jeon, Che-Ok;Choi, Yoon-Ho;Hong, Seung-Pyo;Lee, Seung-Goo;Ha, Jae-Suk;Kang, Gwan-Tae
    • Journal of Microbiology and Biotechnology
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    • v.13 no.6
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    • pp.1013-1017
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    • 2003
  • A thermophilic nonspore-forming rod isolated from hay compost in Korea was subjected to a taxonomic study. The microorganism, designated as $SC-1^T$, was identified as a nitrate-reducing and nonmotile bacterium. Although the strain was negatively Gram-stained, a KOH test showed that the strain $SC-1^T$ belonged to a Gram-positive species. Growth was observed between 45 and $70^{\circ}C$. The optimal growth temperature and pH were $60^{\circ}C$ and pH 7.5, respectively. The G+C content of the genomic DNA was 65 mol% and the major quinone types were MK-6 and MK-7. A phylogenetic analysis based on 16S rDNA sequences revealed that the strain $SC-1^T$ was most closely related to Symbiobacterium thermophilum. However, the level of DNA-DNA relatedness between strain $SC-1^T$ and the type strain for Symbiobacterium thermophilum was approximately 30%. Accordingly, on the basis of the phenotypic traits and molecular systematic data, the strain $SC-1^T$ would appear to represent a new species within the genus Symbiobacterium. The type strain for the new species is named $SC-1^T$ ($=KCTC\;0307BP^T;\;DSM15906^T$).