• 한국식품위생안전성학회지
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• 제12권2호
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• pp.111-116
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• 1997

For the purpose of improving hygienic quality of dried-Angelica Keiskei Koidz powder, the effect of ozone treatment and gamma irradiation on the microbial decontamination and physicochemical properties were investigated. Gamma irradiation at 5 to 7.5 kGy resulted in sterilizing total aerobic bacteria, molds and coliforms below detective levels, while ozone treatment for 8 hours up to 18 ppm did not sufficiently eliminate the total aerobic bacteria of the sample. The physicochemical properties of the sample were not changed by gamma irradiation up to 7.5 kGy, whereas, ozone treatment caused remarkable changes in pH, TBA value, chlorophyll, carotenoid and fatty acid compositions. Therefore, this investigation demonstrated conclusively that gamma irradiation was more effective than ozone treatment for decontaminating and sterilizing the dried-Angelica Keiskei Koidz powder, with minimal effect on the physicochemical properties analyzed.

• 한국식품과학회지
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• 제36권1호
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• pp.174-182
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• 2004

Hygienic standards for pizza specialty restaurant located in Seoul during summer, 2000 were established based on HACCP concept by measuring temperature, time, pH, $A_{w}$ and microbiological assessments of pizza, and evaluation of hygienic conditions of kitchens and workers. Kitchen and worker conditions were average 1.2 and 1.0 (3 point Sly's scale), respectively, Microbial contaminations occurred at $5-60^{\circ}C$, pH above 5.0, and $A_{w}$ (0.93-0.98). Microbial assessments for pizza processing revealed $1.5{\times}10^{2}-3.9{\times}10^{8}\;CFU/g$ of TPC and $0.5{\times}10^{1}-1.6{\times}10^{7}\;CFU/g$ of coliforms, exceeding standards ($TPC\;10^{6}\;CFU/g\;and\;coliform\;10^{3}\;CFU/g$) established by Solberg et al., although significantly decreased after baking. S. aureus was not discovered, but Salmonella was found in onions. Tools and containers such as pizza cutting knife, topping container, serving bowl, pizza plate, working board, and dough kneading board contained $6.2{\times}10^{2}-1.1{\times}10^{9}\;CFU/g$ of TPC, $2.0{\times}10^{1}-6.2{\times}10^{3}\;CFU/g$ of coliforms. Workers' hands contained $3.1{\times}10^{4}\;CFU/g$ of TPC and S. aureus as compared to safety standards of Harrigan and McCance (500 and 10 CFU/g of TPC and coliforms per $100cm^{2}$). CCPs (critical control points) were determined as receiving, topping, and baking according to CCP decision tree analysis. Results suggest purchase of quality materials, careful monitoring of time and temperature, hygienic use of tools and utensils, and sanitary practicer by workers are recommended as control points for safe pizza production.

• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.70-78
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• 2021

Identifying the extracellular metabolites of microorganisms in fresh vegetables is industrially useful for assessing the quality of processed foods. Pectobacterium carotovorum subsp. carotovorum (PCC) is a plant pathogenic bacterium that causes soft rot disease in cabbages. This microbial species in plant tissues can emit specific volatile molecules with odors that are characteristic of the host cell tissues and PCC species. In this study, we used headspace solid-phase microextraction followed by gas chromatography coupled with mass spectrometry (HS-SPME-GC-MS) to identify volatile compounds (VCs) in PCC-inoculated cabbage at different storage temperatures. HS-SPME-GC-MS allowed for recognition of extracellular metabolites in PCC-infected cabbages by identifying specific volatile metabolic markers. We identified 4-ethyl-5-methylthiazole and 3-butenyl isothiocyanate as markers of fresh cabbages, whereas 2,3-butanediol and ethyl acetate were identified as markers of soft rot in PCC-infected cabbages. These analytical results demonstrate a suitable approach for establishing non-destructive plant pathogen-diagnosis techniques as alternatives to standard methods, within the framework of developing rapid and efficient analytical techniques for monitoring plant-borne bacterial pathogens. Moreover, our techniques could have promising applications in managing the freshness and quality control of cabbages.

• 한국식품위생안전성학회지
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• 제33권2호
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• pp.94-101
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• 2018

본 연구에서는 김치 제조시 철저한 재료 세척의 필요성을 확인하고, 더불어 비가열 식품인 김치의 위생 안전성 실태를 파악하여 효율적 미생물 저감화 방안을 마련하고자 국내에서 유통되는 김치 원 부재료 200건의 세척 전과 후의 비교분석과 시판 김치 100건에 대하여 미생물 분석을 실시하였다. 김치 원 부재료 및 완제품에 대하여 일반세균수, 대장균 및 대장균군을 모니터링 하였으며, 병원성 미생물 9종(Bacillus cereus, Clostridium perfringens 정성 정량, Staphylococcus aureus, Enterohemorrhagic Escherichia coli, Campylobacter jejuni/coli, Yersinia enterocolitica, Salmonella spp., Vibrio parahaemolyticus, Listeria monocytogenes 정성)을 분석 하였다. 모니터링 결과, 김치 원 부재료 및 완제품에서 일반세균은 1.4~9.0 log CFU/g 수준으로 확인되었으며, 원 부재료 중 생강에서 8.8 log CFU/g, 완제품 중 총각김치에서 9.0 log CFU/g으로 일반세균이 가장 높게 나타났다. 대장균군은 0.5~7.3 log CFU/g으로 확인되었으며, 생강에서 7.3 log CFU/g으로 가장 높게 나타났고, 병원성 미생물 9종의 분석결과, Bacillus cereus를 제외한 나머지 8종의 식중독균은 모든 시료에서 검출되지 않았다. 김치 제조 시 사용되는 김치 원 부재료들의 세척공정 후 미생물 수는 일반세균수 0.2~3.2 log CFU/g, 대장균군 0.3~2.7 log CFU/g, Bacillus cereus 1.0~3.9 log CFU/g 감소하였다. 따라서, 김치 원 부재료의 세척 공정으로 미생물 오염도를 감소 시켰으며, 이 결과를 통하여 김치 완제품의 위생 및 저장성 증진에 기여 할 수 있을 것으로 확인되었다.

• 한국식품조리과학회지
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• 제18권3호
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• pp.309-318
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• 2002

The purposes of this study were to estimate the microbial and physicochemical quality of vegetable dishes without heat treatment such as sengchae, a traditional Korean vegetable dish, and to suggest a safer method of preparation. Platycodon sengchae and vegetable salad were monitored from the ingredient to final product before serving while storing at different temperature (4, 10$^{\circ}C$) and period (1, 2, 4, 7 days) at foodservice establishments. The results showed that the storage temperature pH, Aw, moisture content, and microbial loads are the important factors affecting the quality of vegetable dishes without heat treatment, and a thorough hygienic management from the purchase to the preparation of the dishes is needed to secure the quality of prepared foods in the foodservice establishments.

• 한국식품조리과학회지
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• 제23권5호
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• pp.749-760
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• 2007

The purpose of this study was to evaluate the microbiological quality of non-heat-processed foods and the effects of HACCP implementation. Here, cabbage salad and cucumber&onion salad were selected and we investigated HA(Hazard Analysis) by checking microbiological quality, time and temperature, pH, and water activity at each processing stage. Thus, the receiving of spices and dressings, washing and sterilizing, cutting, cooking, and serving stages were all considered CCPs. Before implementing HACCP, microbial analysis showed that standard plate counts and coliform counts were higher than standard levels in most of the raw ingredients of each menu, as well as during the production process. The microbiological quality of the utensils and employee's hands used during cooking indicated levels requiring direct management. Evaluations of falling bacteria-in the foodservice establishment work areas ranged from $2{\sim}12CFU/plate$. However, after HACCP implementation, microbiological levels improved to standard levels fly sanitation education. Also, the number of falling bacteria were lower than before implementing HACCP. Therefore, it is essential the foodservice operations make efforts to implement HACCP, so that microbiological hazard levels are lowered and hygienic status improved.

• 한국식품저장유통학회지
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• 제10권3호
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• pp.278-283
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• 2003

김치 제조시 위생적 품질 개선을 위하여 ethanol과 천연항균물질인 polylysine의 복합 첨가를 검토하였다. Ethanol 농도를 0.3, 0.6 및 0.9%로 첨가하여 김치를 제조한 후 1$0^{\circ}C$에 저장하면서 3일 간격으로 총균수, 대장균군, 젖산균, 곰팡이 및 효모 등을 비교 측정한 결과, ethanol의 첨가는 대장균군과 젖산균의 생육 억제에 있어 보다 효과적인 것으로 나타났으며, ethanol 농도 0.6% 첨가시부터 미생물 생육 억제 효과가 뚜렷하게 나타났다. 또한 ethanol 0.6%를 첨가하여 담근 김치는 저장중 pH 저하 및 산도 상승에 대한 지연효과를 보여주었고, 저장중 관능특성의 변화에서도 신냄새, 이취, 신맛, 이미, 조직감 및 종합적 기호도의 모든 항목에서 가장 우수한 것으로 평가되었다. Polylysine은 단일 사용시보다는 0.6% ethanol과 복합 첨가했을 경우 미생물 생육 억제에 상승효과를 보였으나 관능평가에서 이미 및 이취를 느끼는 것으로 평가되어 0.6% ethanol 단일 첨가가 미생물 증식 억제 및 발효 지연에 가장 효과적인 것으로 나타났다.

• 한국식품위생안전성학회지
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• 제16권2호
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• pp.96-102
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• 2001

위생적인 신선육 처리 방안을 모색하기 위하여 도체처리 과정과 식육처리 과정 중 고기의 표면 및 작업도구의 위생실태를 계절별로 검토하였다. 하절기에 총균수, 저온성 균수, 대장균군수 모두 높게 나타났으며, 특히 도체 세척전보다 세척 후에 미생물 오염도가 높았고, 계절에 관계없이 수송 후의 오염도가 가장 높았다. 칼, 도마와 장갑에서 하절기에 $10^{5}$ $ extrm{cm}^2$을 나타내어 높은 미생물 오염도를 나타내었다. 도체 처리 과정 중 도체 표면에서 Escherichia. coli O157, Pseudomonas aeruginosa, Klebsiella, ornithinolytica, Staphylococcus aureus, E. Tatumella, ptyseos, Serratia odorifera, Aeromonas, Aeromonas sobria, Enterobacter colacae, Flavimonas oryzihabitans oryzihabitans를 분리하였으며, 도마에서는 S. aureus, Listeria grayi, L. monocytogenes, 장갑에서 L. grayi, Erwinia spp., Salmonella spp. S aureus, 골발육에서는 Citrobacter freundii, L. momocytogenes, S. aureus가 분리.동정하였다.

• 한국식품과학회지
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• 제32권5호
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• pp.1102-1106
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• 2000

본 연구는 과메기의 위생적 품질 및 저장기간을 연장하기 위한 감마선 조사의 효과를 조사하기 위해 실시하였다. 과메기를 0, 3, 5, 7, 10 kGy의 선량으로 감마선 조사하여 저장하면서 미생물 생육을 검사하였다. 미생물학적 관점에서 비조사구는 저장동안 빠르게 품질이 저하되었고, 선택배지를 이용한 시험에서 병원균으로 추정되는 균들의 생육이 관찰되었다. 그러나, 감마선 조사된 시료에서 총균수 및 병원균으로 추정되는 균들의 생육이 조사선량이 증가함에 따라 감소되었고, 7에서 10 kGy의 감마선 조사가 과메기의 저장에 적절하고 효과적인 선량으로 나타났다.

• 대한가정학회지
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• 제41권10호
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• pp.101-116
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• 2003

The study was conducted to establish hygienic standards of salad items for pizza restaurant located in Seoul by applying HACCP system during the summer of 2000. The study measured temperature, time, pH, Aw and microbial assessments. The hygienic conditions of the kitchen and workers were on the average(1.21, 1.0 out of 3 pts.), but some improvement should be made: separate use of trash can and leftover disposal, separate use of knives and cutting boards, habits for hand washing and wearing hygienic gloves. For salad production, all procedures were peformed under food safety danger zone ($5{\~}60^{\circ}C$). The ingredients were mostly above pH 5.0 and high in Aw($0.94{\~}0.99$). Microbial assessments for salad production revealed that TPC($1.8{\times}10^3{\~}1.0{\times}10^{10}CFU/g$) and coliforms($1.5{\times}10{\~}5.2{\times}10^5 CFU/g$) exceeded the standards by Solberg et al.(TPC: $10^6CFU/g$, coliforms: $10^3CFU/g$). S. aureus was not detected but Salmonella was found in three food items(egg, macaroni and macaroni salad). Moreover, the workers' hands contained 3.1 104 CFU/g of TPC and 4.2 102 CFU/g of S. aureus requiring further remedy since it exceeded the safety standards suggested by Harrigan and McCance (500 CFU/g of TPC per $100cm^2$ and 10 CFU/g of coliforms per $100cm^2$). According to the critical control point(CCP) decision tree analysis, vegetable receiving, vegetable holding, mixing, display on coleslaw, macaroni draining, display on macaroni salad, egg peeling & cutting, apple cutting, and display on salad bar were determined as CCPs. From the findings it would be suggested that purchase of Quality materials, short holding and display time, storing food at right temperature, using sanitary cooking utensils, and improvement of workers' food handing practices are needed to ensure the safe salad production in this specific pizza restaurant.