• Korean Journal of Soil Science and Fertilizer
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• v.37 no.2
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• pp.116-123
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• 2004

This study was conducted to investigate the changes in physicochemical and microbiological properties during fermenting process of organic fertilizer which was made from the mixture of organic materials such as sesame oil cake, fish meal, blood meal, rice bran, ground bone meal, and natural minerals such as illite, crusted oyster shell and loess. They were mixed and fermented for 70 days. The sesame oil cake and rice bran, major ingredients for organic fertilizers, consisted of 7.6 and 2.6% total nitrogen, 3.6 and 4.6% $P_2O_5$, 1.4 and 2.2% $K_2O$, respectively. The ground bone meal included 29.2% $P_2O_5$ and illite included 3.8% $K_2O$. Temperature of organic fertilizer during the fermentation rapidly increased over $50^{\circ}C$ within 2 days after mixing and stabilized similar to outdoor temperature after 40 days. Moisture content decreased from 36.3 to 16.0% after 1 month. C/N ratio of organic fertilizer slightly increased until 30 days and thereafter, it slowly decreased, It resulted from the faster decrease of total nitrogen concentration compared with organic matter. Concentration of $NH_4-N$ in organic fertilizer rapidly increased from 1,504 to $5,530mg\;kg^{-1}$, the highest concentration after 10 days. Meantime, $NO_3-N$ concentration was low and constant about $150mg\;kg^{-1}$ over the whole fermenting period. This result seemed to be due to the high pH. The organic ferfilizer fermented for 70 days was composed of 2.7% N, 2.8% $P_2O_5$, 1.8% $K_2O$, and 35.9% organic matter. Total populations of aerobic bacteria, Bacillus sp. and actinomycetes, after fermenting process, were $12.5{\times}10^{10}$, $45.5{\times}10^{5}$ and $13.6{\times}10^{5}cfu\;g^{-1}$ respectively. Pseudomonas sp. was $71.9{\times}10^{7}cfu\;g^{-1}$ at first, but it rapidly decreased according to the rise of temperature. Yeasts played an important role in the early stage of fermentation and molds did in the late stage.

• Korean Journal of Environmental Agriculture
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• v.27 no.4
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• pp.343-348
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• 2008

To investigate the effects of incorporation of green manures (GM) into a sandy loam soil on growth, yield, and nutrient uptake of tomato (Lycoperiscon esculentum Mill.) and nutrient balances (input minus offtake of nutrients), five tomato production systems were compared under the condition of plastic film house: 1) a no input system (no additional amendment or inputs, 0-To-0-To); 2) a conventional system (application of N-P-K chemical fertilizers, Cf-To-Cf-To); 3) a leguminous GM-containing system (hairy vetch-tomato-soybean-tomato, Hv-To-Sb-To); 4) a graminaceous GM-containing system (rye-tomato-sudan grass-tomato, Ry-To-Sd-To); and 5) system mixed with leguminous and graminaceous GMs (rye-tomatosoybean- tomato, Ry-To-Sb-To). Here, hairy vetch and rye were cultivated as winter cover crops during late $Dec{\sim}late$ Feb and soybean and sudan grass were cultivated as summer cover crops during late $Jun{\sim}mid$ Aug. All of them cut before tomato planting and then incorporated into soil. Biomass of GMs was greater in summer season than that of winter season. Nitrogen amount fixed by a leguminous plants was about $126\;kg\;ha^{-1}$ per a cropping season, corresponding to 60% N level needed for tomato production, which was comparable to 50 and $96\;kg\;ha^{-1}$ fixed by rye and sudan grass. As a result, tomato yield of Hv-To-Sb-To system (legume GM treatment) was similar to Cf-To-Cf-To (conventional), but that in Ry-To-Sd-To system (graminaceous GM treatment) was not attained to a half level of conventional treatment. Nutrient budgets for N, P and K on the conventional farm were balanced or somewhat positive exception for minus-balanced K. Ry-To-Sd-To system showed a positive N, P and K budgets due to the depressed growth of tomato which is caused by high C/N ratio and low N-fixing capacity of the GMs. Inversely, those of Hv-To-Sb-To system were negative in all of N, P and K budgets because of increased growth and yield of tomato with high nitrogen-supplying capacity as well as low C/N ratio of leguminous GM. In conclusion, although conventional cultivation has an advantage in relation to N, P and K nutrient budgets rather than GM-incorporated systems, a leguminous GMs could be recommended as nitrogen reservoir and soil amendment because the yield of tomato between use of leguminous GM and conventional cultivation was not only significantly difference, but also GMs commonly reduce nutrient loss and improve microbial communities.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.447-453
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• 2007

With a broad objective for the development of microbial based fertilizers, a total of 373 strains were isolated from rhizoplane and rhizosphere of pepper, tomato, lettuce, pasture, and grass. The efficacy of the isolates to augument overall plant growth was evaluated. After screening for their plant growth promotion and antagonistic properties in vitro efficient strains were further selected. The most efficient strains was characterized by 16S rRNA gene sequences and biochemical techniques and was designated as Bacillus subtilis S37-2. The strains facilitated plant growth and inhibited the plant phathogenic fungi such as Fusarium oxysporum (KACC 40037, Rhizoctonia solani (KACC 40140), and Sclerotinia sclerotiorum (KACC 40457). Pot based bioassay using lettuce as test plant was conducted by inoculating suspension ($10^5$ to $10^8cells\;mL^{-1}$) of B. subtilis S37-2 to the rhizosphere of lettuce cultivated in soil pots. Compared with non-inoculated pots, marked increase in leaf (42.3%) and root mass (48.7%) was observed in the inoculation group where the 50ml of cell mixture ($8.7{\times}10^8cells\;ml^{-1}$) was applied to the rhizosphere of letuce either once or twice. Antagonistic effects of B. subtilis S37-2 strain on S. sclerotiorum (KACC 40457) were tested. All the tested lettuce plants perished after 9 days in treatment containing only S. sclerotiorum, but only 17% of lettuce was perished in the inoculation plot. B. subtilis grew well in the TSB culture medium. The isolates grew better in yeast extracts than peptone and tryptone as nitrogen source. The growth rate was 2~4 times greater at $37^{\circ}C$ as compared with $30^{\circ}C$ incubation temperature. B. subitlis S37-2 produced $0.1{\mu}g\;ml^{-1}$ of IAA (indole 3-acetic acid) in the TSB medium containing L-tryptophan($20mg\;L^{-1}$) in 24 hours.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.733-743
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• 2020

We estimated the carbon storage of coal mines reclaimed using Betula platyphylla (BP), Pinus koraiensis (PK), and Pinus spp. (PS, Pinus densiflora, Pinus rigida, and Pinus thunbergii). The carbon storage of tree biomass (TB), forest floor(FF), mineral soil (MS), and the total forest were quantified. Reclaimed sites were located in Gangwon-do, Gyeongsangbuk-do, and Jeollanam-do; reclamation was conducted at various times in each region. The carbon storage (ton C ha^-1) in FF (BP: 3.31±0.59, PK: 3.60±0.93, PS: 4.65±0.92), MS (BP: 28.62±2.86, PK: 22.26±5.72, PS: 19.95±3.90), and the total forest(BP: 54.81±7.22, PK: 47.29±8.97, PS: 45.50±6.31) were lower than that of natural forests (NF). The carbon storage in TB was lower in BP (22.57±6.18) compared to NF, while those in PK(21.17±8.76) and PS (20.80±6.40) were higher than in NF. While there were no significant differences in the carbon storage of TB, FF, and the total forest among tree species, results from MS showed a significant difference among species. TB and the total forest carbon storages in all sites increased after reclamation. Soil pH and cation exchange capacity values in BP and PS were lower than in NF. Amounts of labile carbon, available phosphate, and microbial biomass carbon in reclaimed sites were less than half of NF. There are a number of methods that could increase the reclamation efficiency. Applications of lime or organic fertilizers, as well as tillage operations, may improve soil properties in reclaimed coal mines. Additionally, pruning and thinning would increase tree growth thereby increasing carbon storage.

• Korean Journal of Agricultural Science
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• v.17 no.1
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• pp.1-8
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• 1990

The paper board sludge(PBS) itself and compost sludge manure(CSM) mixed with sawdust, fowl droppings and urea to the PBS were treated to soybean plants to find the effects of growing characters, yield components, and nutritional compounds in the plant tissues. 1. Percentages of missing plants were 5-9% and 3.6-4.1% in the treatments of PBS and CSM, respectively. After that, the plants were restored to normal conditions. Anyway it is not desirable to use the paper board sludge and immature compost sludge manure in seeding time or to young seedlings. 2. Growth of the plant height was retarded in early growing phase, but it was normal in later stages. And the width and length of the largest leaf, numbers of main stem nodes and pods were not significant among the treatments. 3. The yield intends to increase through the treatments of 1,200, 1,600, and 2,000Kg PBS per 10a. In the CSM treatments with high rate of sawdust, fowl droppings and urea, the numbers of pods and grains were higher than the treatment of high content of PBS. 100-grain and one liter weights were opposite intention. It was suggested that the excess nitrogen amounts from the compost sludge manure than conventional fertilization affected to the yield components. 4. The contents of the main chemical compounds such as N, $P_2O_5$, $K_2O$, Ca, Mg were determined. The concentrations of nitrogen were higher in the treatment of PBS and CSM than none and control. 5. In conclusion, the nutritional effects of PBS were in evidence. To use the sludges, it should be fermented with adequate additives to improve the aeration, C/N ratio, activity of microbial, and other conditions. The sludges could be used to crops as a fertilizers effectively.