• Title/Summary/Keyword: micro-clustering

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The Potential Probiotic and Functional Health Effects of Lactic Acid Bacteria Isolated from Traditional Korean Fermented Foods (한국 전통발효식품에서 분리한 유산균의 프로바이오틱스 특성 및 건강기능성 연구)

  • Ohn, Jeong-Eun;Seol, Min-Kyeong;Bae, Eun-Yeong;Cho, Young-Je;Jung, Hee-Young;Kim, Byung-Oh
    • Journal of Life Science
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    • v.30 no.7
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    • pp.581-591
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    • 2020
  • This study investigated the probiotic properties and physiological activities of Korean fermented foods such as sikhae, young radish kimchi, and bean-curd dregs. Among the isolated lactic acid bacteria, Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, and Leuconostoc mesenteroides YMT6 demonstrated a greater than 60% survival rate at pH 2.5, along with an excellent survival rate even at 0.3% bile acid. These five bacteria showed strong flocculation ability in autoaggregation and coaggregation tests, indirectly clustering useful micro-organisms and inhibiting the attachment of pathogenic bacteria. In a cell surface hydrophobicity test, these bacteria showed adhesion to three solvents (ethyl acetate, chloroform, and xylene) and high hydrophobicity, thereby indicating excellent indirect cell adhesion to intestinal cells. The cell-free supernatants and intracellular extracts of the five lactic acid bacteria showed antioxidative activity in the form of 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability and lipid peroxidation inhibition. Antimicrobial activities were also observed in four pathogenic bacteria, namely E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, and S. aureus KCTC 1916. These results demonstrate that these five lactic acid bacteria could be used as probiotics with antioxidant and antimicrobial properties.

Delineation of Provenance Regions of Forests Based on Climate Factors in Korea (기상인자(氣象因子)에 의한 우리 나라 산림(山林)의 산지구분(産地區分))

  • Choi, Wan Yong;Tak, Woo Sik;Yim, Kyong Bin;Jang, Suk Seong
    • Journal of Korean Society of Forest Science
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    • v.88 no.3
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    • pp.379-388
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    • 1999
  • As a first step for delineating the provenance regions of the forest trees in Korea, horizontal zones have been deduced primarily from the various climatic factors such as annual mean temperature, extremely low temperature, relative humidity, annual gum of possible growing days, duration of sunshine and dry index. The basic concept to the delineation of the provenance regions was based on the ecological regions, which was likely to be more practical than that on the basis of the typical provenance regions at the species level. Primary classification of the regions has been based on the forest zones(sub-tropical, warm-temperate, mid-temperate and cool-temperate) as a broad geographic region. Further classification has been carried out using cluster analyses among the basic regions within forest zone. On the basis of clustering, a total of 19 regions including 3 from sub-tropical, 6 from warm-temperate, 8 from mid-temperate and 2 from cool-temperate was horizontally delineated. Of the mean values of 6 climate factors at the broad geographic region level, three factors such as annual mean temperature, extremely low temperature, annual growing days showed directional tendencies from subtropical to cool-temperate, while the others didn't. The values of relative humidity, duration of sunshine and dry index varied among the provenance regions within forest zone. These three factors might he more sensitive by the micro-environment condition than by the macro-environment condition. Present study aimed to delineate the primary provenance regions for tentative application to forest practices. These will be stepwise revised through the supplement using accumulated information regard to genecological data.

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