• Proceedings of the PSK Conference
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• 2003.04a
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• pp.284.2-284.2
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• 2003

Using a column-switching technique. highly sensitive and selective semi-micro high-performance liquid chromatographic (HPLC) method has been developed for the determination of baclofen in human plasma. Following precipitation of plasma sample containing baclofen with zinc sulfate-acetonitrile, samples were directly injected on to the system. (omitted)

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.2
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• pp.25-33
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• 1984

The effect of particle size on adsorption of trihalomethane have been studied. Using mean particle sizes ranging from 0.73 to 2.03 mm, physical properties of carbon and trihalomethane adsorption characteristics have been investigated experimentally. With increasing particle size specific surface area and pore volume decreased, and the mean pore radius increased significantly. Large pores were dominant in large particles, while small particles were composed of small pores. Isotherm studies were conducted using static bottle point technique and the results were well described by the Freundlich isotherm equations. The adsorption capacity increased significantly with decreased particle size. Additionally micro-column tests were carried out, and the results were compared with the model simulation. From the micro-column studies it was found out that the film transfer coefficient were almost constant, and the differences in the trihalomethane removal efficiency was mainly due to the differences in the adsorption capacity of the particles of different size.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.585-586
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• 2006

In this paper, we present a design, analysis, fabrication and performance test of the novel DoD metal-jet system for application to the high-density and high-temperature-melting materials. Based on the theoretical analysis, we design the metal-jet print head system and fabricate the metal-jet system, which can eject the droplet of lead-free metal solder in the high-temperature. In the experimental test, we set up the test apparatus for visualization of the droplet ejection and measure the Ejected droplet volume and velocity. As a result, the diameter, volume and the velocity of the ejected droplet are about $65-70{\mu}m$, 145-180 pl and 4m/sec. We also fabricate vertical and inclined 3D micro column structures using the present molten metal inkjet system. The measured geometries of the micro column structures are about height of $2,100{\mu}m$, diameter of $200{\mu}m$ and aspect ratio of 10.5 for vertical micro column and $1,400{\mu}m$ of height and $150{\mu}m$ of diameter for $65^{\circ}$-inclined micro column, respectively.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.05a
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• pp.452-457
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• 2003

As a fundamental study on developing elements with micro shape, micro mold parts machining and experiment of injection molding using it were performed. The ultra precision micro machining system with high functionality was fabricated, and utilized in the machining of micro parts. By using this machining system and micro end-mill tool, a micro circle column structure of high aspect ratio, diameter 60 $\mu\textrm{m}$, height 500 $\mu\textrm{m}$, was fabricated. And a micro lens molds were fabricated by using ball end-mill tool of 300 $\mu\textrm{m}$ diameter and diamond fly-cut tool of 150 $\mu\textrm{m}$ radius. A micro injection molding machine, which is clamping force 1.75 ton, injection capacity 2.8cc, was fabricated for injection molding experiment using micro molds. The injection molding experiment was performed by using the injection molding machine, micro cylinder structures and lens molds. This paper introduces these micro machining system and injection molding machine and demonstrates examples of injection molding using fabricated molds.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.369-375
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• 2002

A sperm factor(s) for oocyte activation during fertilization has not been clearly identified. In this study to elucidate an oocyte activation factor(s), mouse sperm were sonicated and ultra-filtered with a 30 kilo-daltons (KD) cutoff membrane and the ultra-filtrate was then sequentially fractionated over Suporose 12 column and Superdex column, The recovered fractions were micro-injected into Mⅱmouse oocytes and second polar body formation (PBF) was examined. Suporose fraction RV2.10 prepared from sperm extract significantly increased PBF. Of Superdex fractions re-separated from Suporose fraction RV2.10, fraction RV2.12 also had the strongest PBF activity. By analyzing with micro-reverse phase column (URPC), the Superdex fraction RV2.12 appeared to be glutamic acid. In microinjection test, glutamic acid significantly increased PBF. This study suggests that glutamic acid should be a type of sperm factor for second polar body formation related to oocyte activation.

• Journal of the Korean Society of Visualization
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• v.16 no.1
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• pp.48-53
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• 2018

In this study, the effect of injection pressure on the column diameter and droplet velocity of liquid jet with the weakly turbulent Rayleigh-like breakup mode is experimentally studied using digital microscopic holography (DMH). The injection nozzle has the diameter of $50{\mu}m$ and injection pressure is varied from 0.1 to 0.4 MPa. When the micro liquid jet is injected into still air, the double-pulsed holograms was recorded on a CCD sensor and numerically reconstructed in order to obtain well focused images. In this study, the liquid column diameter from $50{\mu}m$ orifice nozzle is shown to be changed slightly but the droplet velocity is increased proportionally as the injection pressure is increased.

• International Journal of Precision Engineering and Manufacturing
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• v.6 no.4
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• pp.49-54
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• 2005

In this paper, micro structuring technique using localized electrochemical deposition (LECD) with ultra short pulses was investigated. Electric field in electrochemical cell was localized near the tool tip end region by applying pulses of a few hundreds of nano second duration, Pt-Ir tip was used as a counter electrode and copper was deposited on the copper substrate in mixed electrolyte of 0.5 M $CuSO_4$ and 0.5 M $H_2SO_4$, The effectiveness of this technique was verified by comparison with ECD using DC voltage. The deposition characteristics such as size, shape, surface, and structural density according to applied voltage and pulse duration were investigated. The proper condition was selected based on the results of the various experiments. Micro columns less than $10{\mu}m$ in diameter were fabricated using this technique. The real 3D micro structures such as micro spring and micro pattern were made by the presented method.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.10a
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• pp.383-384
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• 2009

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.6
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• pp.1208-1211
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• 1999

Semi HPLC using a column switching technique was used to determine the trace content of vitamin B12 in various foods. Total analytical time required less than 20 mins per sample and the recovery ratio was 99.9, 99.6, 100.1 and 99.8% for 1.0, 10.0, 100.0 and 1,000 g/kg, respectively. The content of vitamin B12 in various foods obtained using column switching method showed higher levels compared to labels in dried milk(0.5 g/100g) and in grain products(0.51~34.36 g/100g). Thus, this column switching method was more sensitive, effective and precise than the microbiological analysis currently used to determine the trace compounds like a vitamin B12.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.717-720
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• 2003

A chromogenic biosensor employing microfluidics on a chip has been developed for the determination of high-density lipoprotein (HDL) cholesterol (HDL-C) in human serum. We have investigated a plain and effective method to immobilize enzymes within the microchip without chemically modifying micro-channel or technically micro-fabricating column reactor and fluid channel network. In assessing risk factors of coronary heart disease, a micro-chip system would minimize requirements of instrument and reagent handling.