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A Sperm Factor Inducing Second Polar Body Formation in Mouse Secondayy Oocyte  

Park, Y.S (Lab. Of Reproductive Physiology, Department of Animal Biotechnology, Kyungpook National University)
Min, S.H. (Hana Women's Hospital)
Publication Information
Korean Journal of Animal Reproduction / v.26, no.4, 2002 , pp. 369-375 More about this Journal
Abstract
A sperm factor(s) for oocyte activation during fertilization has not been clearly identified. In this study to elucidate an oocyte activation factor(s), mouse sperm were sonicated and ultra-filtered with a 30 kilo-daltons (KD) cutoff membrane and the ultra-filtrate was then sequentially fractionated over Suporose 12 column and Superdex column, The recovered fractions were micro-injected into Mⅱmouse oocytes and second polar body formation (PBF) was examined. Suporose fraction RV2.10 prepared from sperm extract significantly increased PBF. Of Superdex fractions re-separated from Suporose fraction RV2.10, fraction RV2.12 also had the strongest PBF activity. By analyzing with micro-reverse phase column (URPC), the Superdex fraction RV2.12 appeared to be glutamic acid. In microinjection test, glutamic acid significantly increased PBF. This study suggests that glutamic acid should be a type of sperm factor for second polar body formation related to oocyte activation.
Keywords
Oocyte activation; Second polar body; Sperm factor; Glutamic acid;
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